LOCUS       RC215749                9099 bp    DNA     circular  SYN 
DEFINITION  USP6 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 6 (Tre-2 oncogene) (USP6)
ACCESSION   NM_004505
VERSION     NM_004505.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5256..5347 
                     /label="Myc-DDK"  
     misc_feature    5377..5962 
                     /label="PolyA signal"  
     misc_feature    5434..5453 
                     /label="Restriction Enzyme"  
     misc_feature    6238..6910 
                     /label="ColE1"  
     misc_feature    7340..8134 
                     /label="Kan/Neo"  
     misc_feature    8169..8471 
                     /label="SV40 ori"  
     misc_feature    8557..9034 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5238..5244 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5238 
                     /label="USP6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACATGGTA GAGAATGCAG ATAGTTTGCA GGCACAGGAG CGGAAGGACA 
     1081 TACTTATGAA GTATGACAAG GGACACCGAG CTGGGCTGCC AGAGGACAAG GGGCCTGAGC 
     1141 CCGTTGGAAT CAACAGCAGC ATTGATCGTT TTGGCATTTT GCATGAGACG GAGCTGCCTC 
     1201 CTGTGACTGC ACGGGAGGCG AAGAAAATTC GGCGGGAGAT GACACGAACG AGCAAGTGGA 
     1261 TGGAAATGCT GGGAGAATGG GAGACATATA AGCACAGTAG CAAACTCATA GATCGAGTGT 
     1321 ACAAGGGAAT TCCCATGAAC ATCCGGGGCC CGGTGTGGTC AGTCCTCCTG AACATTCAGG 
     1381 AAATCAAGTT GAAAAACCCC GGAAGATACC AGATCATGAA GGAGAGGGGC AAGAGGTCAT 
     1441 CTGAACACAT CCACCACATC GACCTGGACG TGAGGACGAC TCTCCGGAAC CATGTCTTCT 
     1501 TTAGGGATCG ATATGGAGCC AAGCAGAGGG AACTATTCTA CATCCTCCTG GCCTATTCGG 
     1561 AGTATAACCC GGAGGTGGGC TACTGCAGGG ACCTGAGCCA CATCACCGCC TTGTTCCTCC 
     1621 TTTATCTGCC TGAGGAGGAC GCATTCTGGG CACTGGTGCA GCTGCTGGCC AGTGAGAGGC 
     1681 ACTCCCTGCC AGGATTCCAC AGCCCAAATG GTGGGACAGT CCAGGGGCTC CAAGACCAAC 
     1741 AGGAGCATGT GGTACCCAAG TCACAACCCA AGACCATGTG GCATCAGGAC AAGGAAGGTC 
     1801 TATGCGGGCA GTGTGCCTCG TTAGGCTGCC TTCTCCGGAA CCTGATTGAC GGGATCTCTC 
     1861 TCGGGCTCAC CCTGCGCCTG TGGGACGTGT ATTTGGTGGA AGGAGAACAG GTGTTGATGC 
     1921 CAATAACCAG CATTGCTCTT AAGGTTCAGC AGAAGCGCCT CATGAAGACA TCCAGGTGTG 
     1981 GCCTGTGGGC ACGTCTGCGG AACCAATTCT TCGATACCTG GGCCATGAAC GATGACACCG 
     2041 TGCTCAAGCA TCTTAGGGCC TCTACGAAGA AACTAACAAG GAAGCAAGGG GACCTGCCAC 
     2101 CCCCAGCCAA ACGCGAGCAA GGGTCCTTGG CACCCAGGCC TGTGCCGGCT TCACGTGGTG 
     2161 GGAAGACCCT CTGCAAGGGG TATAGGCAGG CCCCTCCAGG CCCACCAGCC CAGTTCCAGC 
     2221 GGCCCATTTG CTCAGCTTCC CCGCCATGGG CATCTCGTTT TTCCACGCCC TGTCCTGGTG 
     2281 GGGCTGTCCG GGAAGACACG TACCCTGTGG GCACTCAGGG TGTGCCCAGC CTGGCCCTGG 
     2341 CTCAGGGAGG ACCTCAGGGT TCCTGGAGAT TCCTGGAGTG GAAGTCAATG CCCCGGCTCC 
     2401 CAACGGACCT GGATATAGGG GGCCCTTGGT TCCCCCATTA TGATTTTGAA TGGAGCTGCT 
     2461 GGGTCCGTGC CATATCCCAG GAGGACCAGC TGGCCACCTG CTGGCAGGCT GAACACTGCG 
     2521 GAGAGGTTCA CAACAAAGAT ATGAGTTGGC CTGAGGAGAT GTCTTTTACA GCAAATAGTA 
     2581 GTAAAATAGA TAGACAAAAG GTTCCCACAG AAAAGGGAGC CACAGGTCTA AGCAACCTGG 
     2641 GAAACACATG CTTCATGAAC TCAAGCATCC AGTGCGTTAG TAACACACAG CCACTGACAC 
     2701 AGTATTTTAT CTCAGGGAGA CATCTTTATG AACTCAACAG GACAAATCCC ATTGGTATGA 
     2761 AGGGGCATAT GGCTAAATGC TATGGTGATT TAGTGCAGGA ACTCTGGAGT GGAACTCAGA 
     2821 AGAGTGTTGC CCCATTAAAG CTTCGGCGGA CCATAGCAAA ATATGCTCCC AAGTTTGATG 
     2881 GGTTTCAGCA ACAAGACTCC CAAGAACTTC TGGCTTTTCT CTTGGATGGT CTTCATGAAG 
     2941 ATCTCAACCG AGTCCATGAA AAGCCATATG TGGAACTGAA GGACAGTGAT GGCCGACCAG 
     3001 ACTGGGAAGT AGCTGCAGAG GCCTGGGACA ACCATCTAAG AAGAAATAGA TCAATTATTG 
     3061 TGGATTTGTT CCATGGGCAG CTAAGATCTC AAGTCAAATG CAAGACATGT GGGCATATAA 
     3121 GTGTCCGATT TGACCCTTTC AATTTTTTGT CTTTGCCACT ACCAATGGAC AGTTACATGG 
     3181 ACTTAGAAAT AACAGTGATT AAGTTAGATG GTACTACCCC TGTACGGTAT GGACTAAGAC 
     3241 TGAATATGGA TGAAAAGTAC ACAGGTTTAA AAAAACAGCT GAGGGATCTC TGTGGACTTA 
     3301 ATTCAGAACA AATCCTACTA GCAGAAGTAC ATGATTCCAA CATAAAGAAC TTTCCTCAGG 
     3361 ATAACCAAAA AGTACAACTC TCAGTGAGCG GATTTTTGTG TGCATTTGAA ATTCCTGTCC 
     3421 CTTCATCTCC AATTTCAGCT TCTAGTCCAA CACAAATAGA TTTCTCCTCT TCACCATCTA 
     3481 CAAATGGAAT GTTCACCCTA ACTACCAATG GGGACCTACC CAAACCAATA TTCATCCCCA 
     3541 ATGGAATGCC AAACACTGTT GTGCCATGTG GAACTGAGAA GAACTTCACA AATGGAATGG 
     3601 TTAATGGTCA CATGCCATCT CTTCCTGACA GCCCCTTTAC AGGTTACATC ATTGCAGTCC 
     3661 ACCGAAAAAT GATGAGGACA GAACTGTATT TCCTGTCACC TCAGGAGAAT CGCCCCAGCC 
     3721 TCTTTGGAAT GCCATTGATT GTTCCATGCA CTGTGCATAC CCGGAAGAAA GACCTATATG 
     3781 ATGCGGTTTG GATTCAAGTA TCCTGGTTAG CAAGACCACT CCCACCTCAG GAAGCTAGTA 
     3841 TTCATGCCCA GGATCGTGAT AACTGTATGG GCTATCAATA TCCATTCACT CTACGAGTTG 
     3901 TGCAGAAAGA TGGGAACTCC TGTGCTTGGT GCCCACAGTA TAGATTTTGC AGAGGCTGTA 
     3961 AAATTGATTG TGGGGAAGAC AGAGCTTTCA TTGGAAATGC CTATATTGCT GTGGATTGGC 
     4021 ACCCCACAGC CCTTCACCTT CGCTATCAAA CATCCCAGGA AAGGGTTGTA GATAAGCATG 
     4081 AGAGTGTGGA GCAGAGTCGG CGAGCGCAAG CCGAGCCCAT CAACCTGGAC AGCTGTCTCC 
     4141 GTGCTTTCAC CAGTGAGGAA GAGCTAGGGG AAAGTGAGAT GTACTACTGT TCCAAGTGTA 
     4201 AGACCCACTG CTTAGCAACA AAGAAGCTGG ATCTCTGGAG GCTTCCACCC TTCCTGATTA 
     4261 TTCACCTTAA GCGATTTCAA TTTGTAAATG ATCAGTGGAT AAAATCACAG AAAATTGTCA 
     4321 GATTTCTTCG GGAAAGTTTT GATCCGAGTG CTTTTTTGGT ACCACGAGAC CCGGCCCTCT 
     4381 GCCAGCATAA ACCACTCACA CCCCAGGGGG ATGAGCTCTC CAAGCCCAGG ATTCTGGCAA 
     4441 GAGAGGTGAA GAAAGTGGAT GCGCAGAGTT CGGCTGGAAA AGAGGACATG CTCCTAAGCA 
     4501 AAAGCCCATC CTCACTCAGC GCTAACATCA GCAGCAGCCC AAAAGGTTCT CCTTCTTCAT 
     4561 CAAGAAAAAG TGGAACCAGC TGTCCCTCCA GCAAAAACAG CAGCCCTAAT AGCAGCCCAC 
     4621 GGACTTTGGG GAGGAGCAAA GGGAGGCTCC GGCTGCCCCA GATTGGCAGC AAAAATAAGC 
     4681 CGTCAAGTAG TAAGAAGAAC TTGGATGCCA GCAAAGAGAA TGGGGCTGGG CAGATCTGTG 
     4741 AGCTGGCTGA CGCCTTGAGC CGAGGGCATA TGCGGGGGGG CAGCCAACCA GAGCTGGTCA 
     4801 CTCCTCAGGA CCATGAGGTA GCTTTGGCCA ATGGATTCCT TTATGAGCAT GAAGCATGTG 
     4861 GCAATGGCTG TGGCGATGGC TACAGCAATG GTCAGCTTGG AAACCACAGT GAAGAAGACA 
     4921 GCACTGATGA CCAAAGAGAA GACACTCATA TTAAGCCTAT TTATAATCTA TATGCAATTT 
     4981 CATGCCATTC AGGAATTCTG AGTGGGGGCC ATTACATCAC TTATGCCAAA AACCCAAACT 
     5041 GCAAGTGGTA CTGTTATAAT GACAGCAGCT GTGAGGAACT TCACCCTGAT GAAATTGACA 
     5101 CCGACTCTGC CTACATTCTT TTCTATGAGC AGCAGGGGAT AGACTACGCA CAATTTCTGC 
     5161 CAAAGATTGA TGGCAAAAAG ATGGCAGACA CAAGCAGTAC GGATGAAGAC TCTGAGTCTG 
     5221 ATTACGAAAA GTACTCTATG TTACAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5281 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5341 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5401 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5461 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5521 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5581 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5641 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5701 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5761 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5821 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5881 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5941 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6001 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6061 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6121 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6181 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6241 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6301 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6361 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6421 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6481 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6541 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6601 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6661 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6721 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6781 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6841 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6901 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6961 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7021 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7081 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7141 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7201 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7261 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7321 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7381 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7441 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7501 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7561 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7621 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7681 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7741 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7801 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7861 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7921 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7981 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8041 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8101 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8161 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8221 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8281 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8341 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8401 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8461 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8521 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8581 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8641 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8701 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8761 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8821 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8881 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8941 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9001 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9061 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//