LOCUS       RC215728                8658 bp    DNA     circular  SYN 
DEFINITION  SYNPO2 (Myc-DDK-tagged)-Human synaptopodin 2 (SYNPO2), transcript variant 1
ACCESSION   NM_133477
VERSION     NM_133477.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4813..4904 
                     /label="Myc-DDK"  
     misc_feature    4934..5519 
                     /label="PolyA signal"  
     misc_feature    4991..5010 
                     /label="Restriction Enzyme"  
     misc_feature    5795..6467 
                     /label="ColE1"  
     misc_feature    6897..7691 
                     /label="Kan/Neo"  
     misc_feature    7726..8028 
                     /label="SV40 ori"  
     misc_feature    8114..8591 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4803..4811 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4803 
                     /label="SYNPO2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCACAGGG GATTTTATCT GCATTTCCAT GACTGGAGGG GCGCCCTGGG 
     1081 GGTTCAGATT GCAAGGTGGC AAGGAGCAGA AGCAGCCCTT ACAAGTTGCA AAGATTCGAA 
     1141 ATCAGAGCAA AGCCTCTGGG TCTGGGCTCT GTGAGGGAGA TGAAGTGGTT TCCATCAATG 
     1201 GCAACCCTTG TGCAGATCTC ACCTACCCTG AAGTCATCAA GCTCATGGAA AGCATAACAG 
     1261 ACTCTCTCCA AATGCTCATC AAAAGACCAT CCAGTGGAAT AAGTGAGGCT TTGATATCTG 
     1321 AAAATGAAAA CAAAAACCTC GAGCATCTCA CACATGGGGG TTATGTGGAA AGTACCACCC 
     1381 TGCAGATTCG ACCGGCCACA AAGACCCAGT GCACAGAATT CTTCCTCGCC CCTGTCAAGA 
     1441 CTGAAGTTCC CCTAGCTGAG AACCAAAGAA GTGGTCCCGA CTGTGCAGGC AGCTTGAAAG 
     1501 AAGAAACAGG CCCGAGCTAC CAAAGGGCTC CCCAAATGCC TGACTCCCAA AGAGGACGCG 
     1561 TGGCAGAAGA GCTGATCTTA AGGGAGAAGG TAGAAGCGGT ACAGCCTGGG CCTGTGGTTG 
     1621 AGCTGCAACT GTCCCTTTCA CAGGAGAGAC ATAAGGGCGC TAGTGGCCCT TTAGTGGCTC 
     1681 TCCCGGGAGC TGAAAAATCT AAGTCTCCTG ACCCAGACCC TAACTTGTCA CATGACAGGA 
     1741 TTGTCCACAT AAATTCGATC CCTACTAATG AGAAAGCAGA CCCTTTCCTG AGGTCCAGCA 
     1801 AGATAATCCA GATCTCCAGT GGCAGAGAGT TGAGAGTGAT CCAGGAAAGT GAAGCAGGAG 
     1861 ATGCGGGACT GCCCCGGGTG GAAGTGATCC TCGACTGCTC TGACAGGCAG AAGACAGAAG 
     1921 GGTGCAGGCT TCAGGCAGGA AAGGAGTGTG TGGATTCTCC AGTGGAAGGA GGGCAGTCAG 
     1981 AAGCACCTCC TTCTCTGGTA TCCTTTGCCG TCTCATCAGA AGGCACAGAG CAGGGAGAAG 
     2041 ATCCACGCTC GGAAAAAGAT CACAGCAGAC CTCACAAGCA CCGAGCGCGG CATGCACGGC 
     2101 TCAGGAGGAG TGAAAGCCTG TCAGAAAAAC AAGTGAAGGA AGCAAAATCT AAATGCAAAA 
     2161 GCATTGCCCT TCTTCTAACG GATGCTCCCA GCCCCAACTC CAAGGGGGTG TTGATGTTTA 
     2221 AGAAGCGACG TCGGAGGGCC AGGAAATACA CCCTAGTTAG CTACGGTACT GGCGAGCTTG 
     2281 AGCGAGAGGC GGACGAGGAG GAAGAAGGTG ACAAGGAGGA TACATGTGAA GTAGCATTTC 
     2341 TTGGTGCAAG CGAATCAGAG GTGGATGAAG AGTTATTGTC TGACGTTGAC GACAACACAC 
     2401 AAGTTGTGAA CTTTGACTGG GATTCTGGAC TGGTGGACAT TGAAAAGAAA CTGAACAGAG 
     2461 GGGACAAGAT GGAGATGTTA CCAGACACCA CAGGCAAGGG AGCCCTCATG TTTGCCAAGA 
     2521 GGAGGGAGAG AATGGATCAG ATCACAGCCC AAAAAGAAGA GGACAAGGTA GGTGGAACGC 
     2581 CAAGCAGAGA ACAAGATGCT GCCCAGACCG ATGGCCTGAG AACCACGACT TCTTACCAAA 
     2641 GAAAGGAGGA AGAGTCGGTA AGAACGCAGA GCTCTGTGAG CAAAAGCTAC ATCGAGGTGA 
     2701 GTCATGGTCT TGGCCATGTT CCCCAACAGA ATGGCTTCAG TGGGGCATCT GAGACAGCAA 
     2761 ACATCCAGAG GATGGTCCCC ATGAATAGAA CGGCCAAACC CTTCCCAGGG TCTGTGAATC 
     2821 AGCCAGCTAC CCCCTTCTCG CCAACCCGAA ACATGACGAG TCCCATTGCT GACTTTCCTG 
     2881 CACCTCCACC TTACTCTGCA GTCACTCCTC CCCCTGACGC CTTCTCCAGA GGGGTTTCAA 
     2941 GTCCGATTGC TGGCCCAGCA CAGCCCCCTC CATGGCCCCA GCCTGCCCCG TGGTCCCAGC 
     3001 CAGCCTTTTA CGATTCGTCT GAGCGAATAG CTTCCCGAGA TGAGAGGATC TCAGTGCCAG 
     3061 CAAAAAGAAC AGGAATATTG CAGGAGGCCA AAAGGAGAAG CACGACAAAA CCCATGTTTA 
     3121 CTTTTAAAGA GCCCAAAGTA AGCCCAAATC CTGAACTCTT GTCACTCCTT CAAAATTCAG 
     3181 AAGGCAAACG GGGCACTGGA GCTGGAGGTG ATTCCGGACC GGAAGAAGAC TACCTCAGCT 
     3241 TGGGGGCAGA GGCTTGTAAT TTCATGCAAA GCTCCTCTGC CAAACAAAAG ACCCCTCCTC 
     3301 CTGTTGCTCC AAAACCTGCA GTCAAGTCCT CATCCTCCCA ACCAGTAACT CCAGTTTCCC 
     3361 CAGTCTGGTC TCCAGGAGTG GCTCCCACCC AACCTCCTGC CTTCCCCACA TCCAACCCAT 
     3421 CAAAGGGCAC CGTTGTCTCC TCCATCAAAA TAGCCCAGCC TTCTTACCCT CCTGCCCGGC 
     3481 CTGCAAGTAC TTTGAACGTG GCTGGTCCCT TCAAAGGACC ACAAGCAGCA GTAGCCAGTC 
     3541 AGAATTACAC ACCCAAACCA ACAGTTTCCA CACCAACAGT CAATGCTGTT CAGCCTGGTG 
     3601 CAGTGGGACC ATCCAATGAG CTTCCAGGAA TGAGTGGGAG AGGAGCTCAG CTCTTTGCTA 
     3661 AAAGGCAGTC GAGAATGGAG AAGTATGTGG TCGATTCAGA CACGGTGCAG GCCCACGCTG 
     3721 CTCGAGCTCA GTCTCCCACT CCATCTCTCC CGGCCAGTTG GAAGTACTCC TCCAATGTCC 
     3781 GAGCACCTCC TCCTGTGGCC TATAATCCTA TCCACTCGCC GTCTTACCCA CTGGCTGCTC 
     3841 TCAAGTCTCA GCCATCAGCT GCACAGCCCT CCAAAATGGG CAAGAAAAAG GGAAAGAAAC 
     3901 CCCTCAATGC ATTAGATGTC ATGAAGCACC AACCGTATCA GCTCAATGCA TCCTTGTTTA 
     3961 CTTTCCAACC TCCAGATGCA AAGGATGGCC TCCCCCAGAA GTCATCAGTC AAGGTCAATT 
     4021 CAGCCCTGGC CATGAAGCAA GCTCTTCCTC CCCGGCCAGT GAATGCTGCC TCACCTACGA 
     4081 ATGTGCAGGC TTCGTCAGTG TACTCGGTAC CAGCCTATAC CTCTCCTCCT TCCTTCTTTG 
     4141 CAGAGGCCTC CTCACCAGTC AGTGCATCCC CAGTGCCTGT GGGCATTCCC ACCTCGCCAA 
     4201 AGCAAGAATC AGCCTCATCA TCTTATTTTG TGGCACCAAG GCCAAAGTTC TCAGCCAAGA 
     4261 AAAGTGGTGT CACAATTCAG GAGAGTGGGC GCTCCCTTTC TCTTCCTGGA AGATCAGTCC 
     4321 CACCCCCCAT TTCTACATCT CCTTGGGTAT ACCAGCCTAC TTATAGTTAC TCTAGTAAAC 
     4381 CAACCGATGG ACTAGAGAAA GCAAACAAGA GACCAACTCC TTGGGAAGCA GCAGCAAAGT 
     4441 CTCCTCTCGG TCTAGTGGAT GATGCTTTCC AACCCAGAAA CATCCAGGAA TCCATTGTGA 
     4501 CAAATGTGGT TTCAGCAGCT CGGAGGAAGG TGCTTCCAGG GCCTCCAGAG GATTGGAATG 
     4561 AAAGACTGTC CTATATTCCT CAAACCCAGA AGGCCTATAT GGGCTCATGT GGAAGGCAAG 
     4621 AGTATAATGT CACAGCCAAT AATAATATGT CCACCACCTC CCAATATGGT TCACAGTTGC 
     4681 CATATGCATA TTATAGGCAG GCTTCAAGAA ATGATTCTGC AATCATGTCC ATGGAAACCA 
     4741 GGTCTGATTA CTGTCTTCCA GTAGCTGATT ACAACTACAA CCCACACCCA AGGGGATGGA 
     4801 GACGCCAAAC AACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4861 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4921 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4981 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5041 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5101 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5161 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5221 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5281 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5341 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5401 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5461 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5521 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5581 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5641 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5701 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5761 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5821 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5881 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5941 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6001 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6061 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6121 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6181 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6241 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6301 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6361 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6421 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6481 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6541 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6601 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6661 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6721 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6781 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6841 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6901 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6961 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7021 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7081 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7141 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7201 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7261 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7321 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7381 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7441 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7501 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7561 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7621 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7681 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7741 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7801 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7861 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8101 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8161 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8221 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8281 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8341 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8401 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8461 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8521 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8581 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8641 AAATTTAACG CGAATTTT
//