LOCUS       RC215725                6678 bp    DNA     circular  SYN 
DEFINITION  ARSJ (Myc-DDK-tagged)-Human arylsulfatase family, member J (ARSJ)
ACCESSION   NM_024590
VERSION     NM_024590.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2835..2926 
                     /label="Myc-DDK"  
     misc_feature    2956..3541 
                     /label="PolyA signal"  
     misc_feature    3013..3032 
                     /label="Restriction Enzyme"  
     misc_feature    3817..4489 
                     /label="ColE1"  
     misc_feature    4919..5713 
                     /label="Kan/Neo"  
     misc_feature    5748..6050 
                     /label="SV40 ori"  
     misc_feature    6136..6613 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2817..2823 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2817 
                     /label="ARSJ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCCCAGG GGCTGTGCGG GGCATCCGCC TCCGCCTTCT CCACAGGCCT 
     1081 GTGTCTGTCC TGGAAAGATG CTAGCAATGG GGGCGCTGGC AGGATTCTGG ATCCTCTGCC 
     1141 TCCTCACTTA TGGTTACCTG TCCTGGGGCC AGGCCTTAGA AGAGGAGGAA GAAGGGGCCT 
     1201 TACTAGCTCA AGCTGGAGAG AAACTAGAGC CCAGCACAAC TTCCACCTCC CAGCCCCATC 
     1261 TCATTTTCAT CCTAGCGGAT GATCAGGGAT TTAGAGATGT GGGTTACCAC GGATCTGAGA 
     1321 TTAAAACACC TACTCTTGAC AAGCTCGCTG CCGAAGGAGT TAAACTGGAG AACTACTATG 
     1381 TCCAGCCTAT TTGCACACCA TCCAGGAGTC AGTTTATTAC TGGAAAGTAT CAGATACACA 
     1441 CCGGACTTCA ACATTCTATC ATAAGACCTA CCCAACCCAA CTGTTTACCT CTGGACAATG 
     1501 CCACCCTACC TCAGAAACTG AAGGAGGTTG GATATTCAAC GCATATGGTC GGAAAATGGC 
     1561 ACTTGGGTTT TTACAGAAAA GAATGCATGC CCACCAGAAG AGGATTTGAT ACCTTTTTTG 
     1621 GTTCCCTTTT GGGAAGTGGG GATTACTATA CACACTACAA ATGTGACAGT CCTGGGATGT 
     1681 GTGGCTATGA CTTGTATGAA AACGACAATG CTGCCTGGGA CTATGACAAT GGCATATACT 
     1741 CCACACAGAT GTACACTCAG AGAGTACAGC AAATCTTAGC TTCCCATAAC CCCACAAAGC 
     1801 CTATATTTTT ATATATTGCC TATCAAGCTG TTCATTCACC ACTGCAAGCT CCTGGCAGGT 
     1861 ATTTCGAACA CTACCGATCC ATTATCAACA TAAACAGGAG GAGATATGCT GCCATGCTTT 
     1921 CCTGCTTAGA TGAAGCAATC AACAACGTGA CATTGGCTCT AAAGACTTAT GGTTTCTATA 
     1981 ACAACAGCAT TATCATTTAC TCTTCAGATA ATGGTGGCCA GCCTACGGCA GGAGGGAGTA 
     2041 ACTGGCCTCT CAGAAGTAGC AAAGGAACAT ATTGGGAAGG AGGGATCCGG GCTGTAGGCT 
     2101 TTGTGCATAG CCCACTTCTG AAAAACAAGG GAACAGTGTG TAAGGAACTT GTGCACATCA 
     2161 CTGACTGGTA CCCCACTCTC ATTTCACTGG CTGAAGGACA GATTGATGAG GACATTCAAC 
     2221 TAGATGGCTA TGATATCTGG GAGACCATAA GTGAGGGTCT TCGCTCACCC CGAGTAGATA 
     2281 TTTTGCATAA CATTGACCCC ATATACACCA AGGCAAAAAA TGGCTCCTGG GCAGCAGGCT 
     2341 ATGGGATCTG GAACACTGCA ATCCAGTCAG CCATCAGAGT GCAGCACTGG AAATTGCTTA 
     2401 CAGGAAATCC TGGCTACAGC GACTGGGTCC CCCCTCAGTC TTTCAGCAAC CTGGGACCGA 
     2461 ACCGGTGGCA CAATGAACGG ATCACCTTGT CAACTGGCAA AAGTGTATGG CTTTTCAACA 
     2521 TCACAGCCGA CCCATATGAG AGGGTGGACC TATCTAACAG GTATCCAGGA ATCGTGAAGA 
     2581 AGCTCCTACG GAGGCTCTCA CAGTTCAACA AAACTGCAGT GCCGGTCAGG TATCCCCCCA 
     2641 AAGACCCCAG AAGTAACCCT AGGCTCAATG GAGGGGTCTG GGGACCATGG TATAAAGAGG 
     2701 AAACCAAGAA AAAGAAGCCA AGCAAAAATC AGGCTGAGAA AAAGCAAAAG AAAAGCAAAA 
     2761 AAAAGAAGAA GAAACAGCAG AAAGCAGTCT CAGGTTCAAC TTGCCATTCA GGTGTTACTT 
     2821 GTGGAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     2881 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     2941 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3001 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3061 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3121 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3181 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3241 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3301 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3361 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3421 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3481 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3541 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3601 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3661 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     3721 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     3781 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     3841 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     3901 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     3961 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4021 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4081 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4141 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4201 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4261 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4321 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4381 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4441 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4501 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4561 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4621 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4681 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     4741 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     4801 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     4861 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     4921 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     4981 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5041 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5101 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5161 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5221 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5281 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5341 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5401 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5461 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5521 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5581 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5641 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     5701 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     5761 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     5821 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     5881 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     5941 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6001 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6061 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6121 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6181 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6241 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6301 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6361 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6421 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6481 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6541 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6601 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6661 AAATTTAACG CGAATTTT
//