LOCUS       RC215720                8064 bp    DNA     circular  SYN 
DEFINITION  ZNF451 (Myc-DDK-tagged)-Human zinc finger protein 451 (ZNF451), transcript variant 1
ACCESSION   NM_001031623
VERSION     NM_001031623.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4221..4312 
                     /label="Myc-DDK"  
     misc_feature    4342..4927 
                     /label="PolyA signal"  
     misc_feature    4399..4418 
                     /label="Restriction Enzyme"  
     misc_feature    5203..5875 
                     /label="ColE1"  
     misc_feature    6305..7099 
                     /label="Kan/Neo"  
     misc_feature    7134..7436 
                     /label="SV40 ori"  
     misc_feature    7522..7999 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4203..4209 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4203 
                     /label="ZNF451"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGACCCG GGGTCGGAAA TAATAGAATC TGTCCCTCCA GCTGGCCCTG 
     1081 AGGCATCTGA GTCAACAACG GATGAAAATG AAGACGACAT TCAGTTTGTC AGTGAAGGAC 
     1141 CATTACGACC TGTTCTTGAA TACATTGATC TGGTCAGCAG TGATGATGAA GAGCCTAGCA 
     1201 CCTCTTATAC TGATGAGAAT ATTAAACGTA AAGACCATAT TGATTATCAG AAGGATAAAG 
     1261 TTGCTTTAAC TCTGGCTCGT CTAGCCCGCC ATGTTGAAGT GGAGAAACAG CAGAAAGAAG 
     1321 AGAAGAATAG AGCATTCAGA GAAAAAATTG ATTTTCAGCA TGCTCATGGG TTACAAGAAT 
     1381 TGGAATTTAT TCGAGGACAT TCTGATACAG AAGCAGCAAG ACTGTGTGTG GACCAGTGGC 
     1441 TAAAAATGCC AGGACTCAAA ACAGGCACAA TTAATTGTGG AACAAAAAGT TCATTCCGAA 
     1501 GAGGAGGCCA CACGTGGGTG TCTGGGAAAC CAATTTTATG TCCTATAATG CACTGTAACA 
     1561 AGGAGTTTGA CAATGGGCAC CTTCTCTTAG GACATTTGAA AAGGTTCGAT CACTCTCCAT 
     1621 GTGATCCAAC AATTACACTA CATGGACCTT TCTTCAGCTC CTTTGCTTGT GTAGTATGTT 
     1681 ATAAAAAATT TGTTACTCAA CAACAATATA GAGATCACCT TTTTGATAAG GAAGCCACAG 
     1741 ATGATGGACA TAACAACAAC CTTCTTCCTC AGATTATTCA GTGTTTTGCA TGTCCAAATT 
     1801 GCTTCCTTCT TTTTAGCAGA AAGGAGGAGT GTTCAAAGCA TATGTCTGGA AAGAATCATT 
     1861 TCCATCAGAG TTTCAAACTG GGTGATAACA AAGGAATTGC ACATCCAATA TCTTTCCCAT 
     1921 CTTTTGCAAA GAAACTTTTG ATCTCTCTGT GCAAAGATGT TCCCTTTCAA GTTAAGTGTG 
     1981 TGGCCTGCCA CAAGACACTG CGTTCCCACA TGGAGCTCAC TGCCCATTTC AGAGTTCATT 
     2041 GTCGAAATGC TGGACCTGTA GCTGTAGCTG AGAAGAGCAT TACCCAGGTT GCAGAGAAAT 
     2101 TCATATTAAG AGGTTATTGT CCAGATTGCA ATCAAGTCTT TGTGGATGAA ACCAGCACCC 
     2161 AAAATCATAA GCAGAATTCA GGACACAAAG TCCGAGTCAT TAACTCAGTG GAAGAATCAG 
     2221 TCTTACTCTA TTGCCACAGC AGCGAAGGGA ACAAGGATCC TTCTTCTGAC TTGCATTTAT 
     2281 TGTTGGATCA ATCAAAATTT TCATCACTTA AAAGAACCAT GTCTATTAAA GAATCTAGCT 
     2341 CACTGGAGTG CATTGCCATT CCAAAAAAGA AGATGAATTT AAAAGATAAA AGCCATGAAG 
     2401 GTGTTGCTTG TGTCCAGAAA GAAAAATCAG TAGTTAAAAC CTGGTTCTGT GAATGCAATC 
     2461 AGCGATTCCC AAGTGAAGAT GCAGTAGAAA AGCATGTTTT CTCAGCAAAC ACAATGGGTT 
     2521 ATAAATGTGT GGTCTGTGGA AAGGTATGTG ATGATTCAGG GGTCATTCGT TTACACATGA 
     2581 GCCGGATTCA CGGAGGGGCA CATTTAAATA ACTTTCTTTT CTGGTGTCGG ACATGCAAAA 
     2641 AGGAGTTAAC AAGGAAAGAT ACTATCATGG CACATGTGAC TGAATTTCAT AATGGACACA 
     2701 GATATTTTTA TGAGATGGAT GAGGTAGAAG GTGAAACTTT GCCATCATCC TCTACAACAT 
     2761 TGGATAATTT GACTGCTAAC AAGCCTTCAT CAGCTATTAC TGTTATTGAT CATTCCCCGG 
     2821 CAAATAGTTC TCCGAGGGGT AAATGGCAAT GCCGGATTTG TGAAGATATG TTTGATTCCC 
     2881 AGGAATATGT AAAACAGCAC TGCATGTCTT TGGCAAGCCA CAAGTTTCAT AGATACAGCT 
     2941 GTGCTCACTG CAGAAAGCCT TTTCATAAGA TAGAAACATT GTACCGACAT TGCCAAGATG 
     3001 AGCATGACAA TGAGATAAAG ATTAAATACT TCTGTGGGCT TTGTGATCTT ATCTTTAATG 
     3061 TGGAAGAAGC ATTTCTGAGT CATTATGAGG AGCACCACAG CATAGATTAT GTATTTGTGT 
     3121 CAGAAAAAAC TGAAACTTCA ATTAAAACCG AAGATGATTT TCCAGTAATA GAGACCAGTA 
     3181 ACCAGTTAAC TTGTGGTTGC CGTGAGAGTT ACATCTGTAA AGTCAACAGA AAAGAAGATT 
     3241 ATAGCAGATG TCTCCAAATC ATGCTGGATA AAGGAAAACT GTGGTTTCGC TGCAGTTTAT 
     3301 GTTCGGCAAC AGCACAGAAT TTAACCGACA TGAACACTCA TATCCATCAA GTGCACAAAG 
     3361 AAAAGAGTGA TGAGGAGGAG CAGCAGTATG TAATCAAGTG TGGCACCTGC ACCAAAGCAT 
     3421 TTCATGATCC TGAGAGTGCA CAGCAGCATT TCCATAGAAA ACATTGCTTC TTACAGAAAC 
     3481 CCAGTGTGGC TCATTTTGGA TCTGAAAAAT CAAACCTGTA CAAGTTTACT GCTAGTGCCT 
     3541 CACATACAGA GAGAAAACTG AAACAGGCAA TAAACTATTC AAAAAGTTTA GACATGGAGA 
     3601 AAGGAGTTGA GAATGACCTA AGCTATCAGA ATATAGAGGA AGAAATTGTT GAGCTTCCAG 
     3661 ATTTGGATTA CCTGCGAACC ATGACTCATA TAGTCTTTGT AGATTTTGAT AACTGGTCAA 
     3721 ACTTTTTTGG TCATCTACCA GGGCATCTAA ACCAAGGAAC ATTTATTTGG GGCTTTCAAG 
     3781 GAGGAAACAC CAATTGGAAG CCTCCGCTCA ACTGTAAGAT TTATAACTAC CTGAACAGGA 
     3841 TTGGATGCTT CTTCCTTCAT CCTCGCTGTA GTAAAAGAAA AGATGCTGCT GATTTTGCCA 
     3901 TATGTATGCA TGCTGGCCGT CTAGATGAAC AACTACCCAA GCAAATTCCT TTCACCATCC 
     3961 TCTCAGGAGA TCAAGGTTTT CTGGAGCTAG AGAATCAATT TAAGAAGACT CAGAGGCCAG 
     4021 CTCATATACT AAACCCTCAC CACTTAGAGG GAGATATGAT GTGTGCCTTG TTAAATAGCA 
     4081 TATCTGATAC CACCAAAGAA TGTGACAGTG ATGATAACAT GGGTGCCAAA AATACTTCAA 
     4141 TAGGAGAAGA ATTTATATCC ACAGAAGATG TGGAATTAGA AGAAGCTATT AGAAGAAGTC 
     4201 TTGAGGAAAT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4261 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4321 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4381 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4441 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4501 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4561 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4621 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4681 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4741 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4801 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4861 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4921 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4981 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5041 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5101 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5161 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5221 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5281 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5341 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5401 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5461 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5521 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5581 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5641 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5701 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5761 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5821 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5881 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5941 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6001 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6061 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6121 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6181 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6241 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6301 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6361 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6421 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6481 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6541 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6601 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6661 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6721 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6781 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6841 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6901 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6961 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7021 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7081 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7141 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7201 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7261 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7321 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7381 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7501 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7561 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7621 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7681 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7741 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7801 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7861 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7921 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7981 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8041 TAACAAAAAT TTAACGCGAA TTTT
//