LOCUS       RC215703                8151 bp    DNA     circular  SYN 
DEFINITION  PALM2 (Myc-DDK-tagged)-Human PALM2-AKAP2 readthrough (PALM2-AKAP2), transcript variant 2
ACCESSION   NM_147150
VERSION     NM_147150.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4308..4399 
                     /label="Myc-DDK"  
     misc_feature    4429..5014 
                     /label="PolyA signal"  
     misc_feature    4486..4505 
                     /label="Restriction Enzyme"  
     misc_feature    5290..5962 
                     /label="ColE1"  
     misc_feature    6392..7186 
                     /label="Kan/Neo"  
     misc_feature    7221..7523 
                     /label="SV40 ori"  
     misc_feature    7609..8086 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4290..4296 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4290 
                     /label="PALM2-AKAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGGCG GAATTGCACA AGGAAAGGCT GCAAGCCATA GCAGAAAAAA 
     1081 GAAAGAGGCA GACTGAAATA GAAGGCAAGC GACAACAGCT TGACGAGCAG ATACTTCTGC 
     1141 TGCAGCATTC CAAGTCCAAA GTGCTTCGGG AGAAATGGCT GCTGCAGGGC ATACCCGCTG 
     1201 GAACTGCCGA AGAGGAGGAA GCCAGGAGGC GGCAGTCTGA AGAGGATGAG TTCAGAGTCA 
     1261 AGCAACTTGA AGATAACATT CAGAGGCTGG AGCAAGAAAT ACAAACGCTA GAAAGTGAAG 
     1321 AGTCCCAGAT ATCTGCCAAA GAGCAAATCA TCCTAGAGAA ACTGAAGGAA ACAGAAAAAT 
     1381 CCTTCAAGGA CTTTCAGAAG GGTTTCTCCA GTACGGATGG AGATGCAGTA AATTACATTT 
     1441 CCTCCCAGCT TCCCGACCTG CCAATCCTCT GTTCACGAAC AGCAGAACCA TCACCTGGGC 
     1501 AGGACGGGAC CAGCAGAGCG GCTGGAGTCG GGTGGGAGAA TGTGCTGCTA AAGGAAGGTG 
     1561 AGTCAGCCTC GAACGCCACA GAAACATCCG GCCCAGACAT GACTATCAAG AAGCCTCCCC 
     1621 AGCTTTCTGA GGATGATATC TGGCTAAAAA GCGAGGGAGA CAACTATAGT GCCACCCTCC 
     1681 TGGAGCCTGC TGCCAGCTCT CTTTCCCCAG ATCACAAAAA CATGGAAATT GAGGTGTCTG 
     1741 TTGCAGAATG TAAAAGTGTT CCTGGAATCA CCTCTACCCC ACATCCCATG GACCATCCCT 
     1801 CCGCTTTCTA TTCACCCCCG CATAATGGCC TCCTTACTGA TCACCACGAA TCCCTGGATA 
     1861 ATGATGTTGC CAGAGAGATC CGCTATCTAG ATGAGGTGCT AGAGGCCAAC TGCTGTGATT 
     1921 CTGCTGTGGA TGGAACGTAC AATGGAACAT CCTCCCCAGA GCCTGGTGCA GTGGTTCTGG 
     1981 TGGGCGGCCT AAGCCCCCCT GTCCACGAGG CGACCCAGCC AGAACCCACT GAAAGAACAG 
     2041 CTAGCCGGCA GGCACCTCCT CACATCGAGC TCAGTAATAG CAGCCCTGAC CCCATGGCAG 
     2101 AGGCAGAAAG AACAAATGGC CATTCCCCCA GCCAGCCTAG AGATGCGCTG GGGGACAGCC 
     2161 TGCAGGTGCC TGTCAGCCCC AGCTCCACAA CCAGCTCACG GTGTTCTTCC CGAGATGGAG 
     2221 AGTTCACTCT CACCACACTG AAAAAGGAGG CCAAGTTTGA GCTGCGTGCC TTCCATGAGG 
     2281 ACAAGAAGCC CTCCAAGCTC TTTGAGGATG ACGAGCATGA GAAAGAACAA TACTGCATTA 
     2341 GAAAAGTGAG GCCTTCAGAG GAGATGCTGG AGCTGGAAAA GGAGAGGAGA GAGCTCATCC 
     2401 GCAGCCAGGC CGTCAAGAAG AATCCTGGCA TTGCAGCAAA ATGGTGGAAT CCCCCGCAGG 
     2461 AAAAAACCAT CGAGGAGCAG CTGGACGAGG AACATCTGGA GTCGCACAAA AAGTACAAGG 
     2521 AGCGCAAAGA GAGAAGGGCA CAGCAGGAAC AGTTGCTGCT GCAGAAGCAG TTACAGCAGC 
     2581 AGCAGCAGCA GCCCCCATCG CAGCTCTGCA CAGCCCCTGC CTCTTCTCAT GAACGCGCAA 
     2641 GCATGATTGA CAAAGCAAAG GAGGACATTG TCACAGAGCA GATAGATTTC TCTGCTGCTC 
     2701 GCAAACAATT TCAGCTGATG GAGAATTCCA GGCAAGCGGT GGCCAAGGGC CAGAGTACAC 
     2761 CCAGGCTGTT CTCCATCAAG CCTTTCTACA GGCCTCTGGG GTCAGTCAAC TCAGACAAGC 
     2821 CACTGACTAA TCCGAGACCA CCTTCTGTCG GGGGACCTCC AGAAGACAGT GGTGCCTCAG 
     2881 CCGCCAAGGG ACAGAAATCC CCCGGTGCCC TGGAGACCCC ATCGGCAGCA GGAAGCCAGG 
     2941 GCAACACAGC CTCTCAGGGG AAGGAAGGGC CCTACAGCGA GCCTTCTAAA CGTGGGCCCT 
     3001 TATCTAAACT GTGGGCTGAG GATGGAGAAT TTACGAGCGC CCGGGCTGTC CTCACTGTGG 
     3061 TCAAGGATGA TGACCATGGG ATTTTGGATC AGTTCTCAAG ATCTGTCAAT GTCTCCTTGA 
     3121 CCCAAGAGGA GCTTGACTCT GGTCTGGACG AATTGTCGGT GAGGTCTCAG GATACCACAG 
     3181 TCCTGGAGAC CCTATCCAAT GATTTCAGCA TGGACAACAT CAGTGACAGC GGGGCATCCA 
     3241 ATGAGACAAC CAATGCCCTC CAGGAAAATT CACTGGCTGA TTTTTCTCTG CCCCAGACAC 
     3301 CACAAACTGA CAACCCCTCA GAGGGCCGAG GAGAAGGCGT CTCCAAGTCA TTTAGTGATC 
     3361 ATGGTTTCTA TTCCCCTTCC TCCACGCTGG GGGACTCTCC GTTGGTTGAT GACCCCTTGG 
     3421 AGTATCAGGC TGGCCTCCTG GTGCAGAATG CCATTCAACA AGCCATAGCC GAGCAGGTGG 
     3481 ATAAAGCTGT GTCCAAAACC AGCAGGGATG GAGCAGAGCA ACAGGGACCT GAAGCGACTG 
     3541 TAGAGGAAGC TGAAGCTGCG GCTTTCGGCT CAGAAAAGCC TCAGAGCATG TTTGAGCCAC 
     3601 CTCAGGTGTC TTCTCCTGTT CAAGAGAAAA GGGATGTATT ACCAAAGATT CTGCCTGCTG 
     3661 AAGACAGGGC GCTCAGGGAA AGGGGGCCCC CCCAGCCACT GCCAGCTGTG CAGCCCAGTG 
     3721 GCCCGATTAA CATGGAGGAG ACCAGGCCCG AAGGAAGCTA TTTCAGCAAG TACTCGGAGG 
     3781 CAGCTGAGCT GAGAAGCACA GCCTCCCTCC TGGCCACTCA AGAATCTGAC GTGATGGTTG 
     3841 GGCCTTTCAA GCTGAGGTCC AGGAAACAGC GGACTTTGTC CATGATTGAG GAAGAGATCC 
     3901 GAGCAGCTCA GGAAAGGGAA GAGGAGCTGA AGAGGCAGAG ACAAGTCTTG CAGAGTACGC 
     3961 AGAGCCCCAG GACAAAGAAT GCCCCATCAC TGCCCTCCAG AACATGCTAC AAAACTGCTC 
     4021 CAGGGAAAAT AGAGAAAGTC AAACCTCCTC CATCCCCCAC CACTGAAGGC CCCAGCTTGC 
     4081 AGCCTGACTT AGCCCCTGAA GAGGCTGCCG GAACCCAGCG GCCCAAGAAT CTGATGCAGA 
     4141 CCCTCATGGA AGACTATGAG ACACACAAAT CTAAAAGGCG CGAGAGAATG GATGATAGTA 
     4201 GTGTCCTCGA GGCCACACGG GTTAATCGAA GAAAGAGCGC ACTGGCTTTG CGCTGGGAAG 
     4261 CAGGGATCTA TGCCAACCAG GAGGAAGAAG ACAACGAAAC GCGTACGCGG CCGCTCGAGC 
     4321 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4381 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4441 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4501 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4561 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4621 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4681 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4741 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4801 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4861 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4921 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4981 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5041 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5161 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5221 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5281 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5341 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5461 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5521 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5581 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5641 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5761 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5821 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5881 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5941 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6001 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6061 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6121 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6181 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6241 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6301 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6361 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6421 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6481 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6541 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6601 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6661 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6721 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6781 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6841 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6901 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6961 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7021 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7081 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7141 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7201 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7261 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7321 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7381 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7561 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7621 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7681 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7741 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7801 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7861 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7921 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7981 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8041 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8101 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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