LOCUS       RC215691                9027 bp    DNA     circular  SYN 
DEFINITION  INSR (Myc-DDK-tagged)-Human insulin receptor (INSR), transcript variant 1
ACCESSION   NM_000208
VERSION     NM_000208.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5184..5275 
                     /label="Myc-DDK"  
     misc_feature    5305..5890 
                     /label="PolyA signal"  
     misc_feature    5362..5381 
                     /label="Restriction Enzyme"  
     misc_feature    6166..6838 
                     /label="ColE1"  
     misc_feature    7268..8062 
                     /label="Kan/Neo"  
     misc_feature    8097..8399 
                     /label="SV40 ori"  
     misc_feature    8485..8962 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5166..5172 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5166 
                     /label="INSR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCGGG GGCCGGCGGG GGGCGGCGGC CGCGCCGCTG CTGGTGGCGG 
     1081 TGGCCGCGCT GCTACTGGGC GCCGCGGGCC ACCTGTACCC CGGAGAGGTG TGTCCCGGCA 
     1141 TGGATATCCG GAACAACCTC ACTAGGTTGC ATGAGCTGGA GAATTGCTCT GTCATCGAAG 
     1201 GACACTTGCA GATACTCTTG ATGTTCAAAA CGAGGCCCGA AGATTTCCGA GACCTCAGTT 
     1261 TCCCCAAACT CATCATGATC ACTGATTACT TGCTGCTCTT CCGGGTCTAT GGGCTCGAGA 
     1321 GCCTGAAGGA CCTGTTCCCC AACCTCACGG TCATCCGGGG ATCACGACTG TTCTTTAACT 
     1381 ACGCGCTGGT CATCTTCGAG ATGGTTCACC TCAAGGAACT CGGCCTCTAC AACCTGATGA 
     1441 ACATCACCCG GGGTTCTGTC CGCATCGAGA AGAACAATGA GCTCTGTTAC TTGGCCACTA 
     1501 TCGACTGGTC CCGTATCCTG GATTCCGTGG AGGATAATTA CATCGTGTTG AACAAAGATG 
     1561 ACAACGAGGA GTGTGGAGAC ATCTGTCCGG GTACCGCGAA GGGCAAGACC AACTGCCCCG 
     1621 CCACCGTCAT CAACGGGCAG TTTGTCGAAC GATGTTGGAC TCATAGTCAC TGCCAGAAAG 
     1681 TTTGCCCGAC CATCTGTAAG TCACACGGCT GCACCGCCGA AGGCCTCTGT TGCCACAGCG 
     1741 AGTGCCTGGG CAACTGTTCT CAGCCCGACG ACCCCACCAA GTGCGTGGCC TGCCGCAACT 
     1801 TCTACCTGGA CGGCAGGTGT GTGGAGACCT GCCCGCCCCC GTACTACCAC TTCCAGGACT 
     1861 GGCGCTGTGT GAACTTCAGC TTCTGCCAGG ACCTGCACCA CAAATGCAAG AACTCGCGGA 
     1921 GGCAGGGCTG CCACCAGTAC GTCATTCACA ACAACAAGTG CATCCCTGAG TGTCCCTCCG 
     1981 GGTACACGAT GAATTCCAGC AACTTGCTGT GCACCCCATG CCTGGGTCCC TGTCCCAAGG 
     2041 TGTGCCACCT CCTAGAAGGC GAGAAGACCA TCGACTCGGT GACGTCTGCC CAGGAGCTCC 
     2101 GAGGATGCAC CGTCATCAAC GGGAGTCTGA TCATCAACAT TCGAGGAGGC AACAATCTGG 
     2161 CAGCTGAGCT AGAAGCCAAC CTCGGCCTCA TTGAAGAAAT TTCAGGGTAT CTAAAAATCC 
     2221 GCCGATCCTA CGCTCTGGTG TCACTTTCCT TCTTCCGGAA GTTACGTCTG ATTCGAGGAG 
     2281 AGACCTTGGA AATTGGGAAC TACTCCTTCT ATGCCTTGGA CAACCAGAAC CTAAGGCAGC 
     2341 TCTGGGACTG GAGCAAACAC AACCTCACCA TCACTCAGGG GAAACTCTTC TTCCACTATA 
     2401 ACCCCAAACT CTGCTTGTCA GAAATCCACA AGATGGAAGA AGTTTCAGGA ACCAAGGGGC 
     2461 GCCAGGAGAG AAACGACATT GCCCTGAAGA CCAATGGGGA CCAGGCATCC TGTGAAAATG 
     2521 AGTTACTTAA ATTTTCTTAC ATTCGGACAT CTTTTGACAA GATCTTGCTG AGATGGGAGC 
     2581 CGTACTGGCC CCCCGACTTC CGAGACCTCT TGGGGTTCAT GCTGTTCTAC AAAGAGGCCC 
     2641 CTTATCAGAA TGTGACGGAG TTCGACGGGC AGGATGCGTG TGGTTCCAAC AGTTGGACGG 
     2701 TGGTAGACAT TGACCCACCC CTGAGGTCCA ACGACCCCAA ATCACAGAAC CACCCAGGGT 
     2761 GGCTGATGCG GGGTCTCAAG CCCTGGACCC AGTATGCCAT CTTTGTGAAG ACCCTGGTCA 
     2821 CCTTTTCGGA TGAACGCCGG ACCTATGGGG CCAAGAGTGA CATCATTTAT GTCCAGACAG 
     2881 ATGCCACCAA CCCCTCTGTG CCCCTGGATC CAATCTCAGT GTCTAACTCA TCATCCCAGA 
     2941 TTATTCTGAA GTGGAAACCA CCCTCCGACC CCAATGGCAA CATCACCCAC TACCTGGTTT 
     3001 TCTGGGAGAG GCAGGCGGAA GACAGTGAGC TGTTCGAGCT GGATTATTGC CTCAAAGGGC 
     3061 TGAAGCTGCC CTCGAGGACC TGGTCTCCAC CATTCGAGTC TGAAGATTCT CAGAAGCACA 
     3121 ACCAGAGTGA GTATGAGGAT TCGGCCGGCG AATGCTGCTC CTGTCCAAAG ACAGACTCTC 
     3181 AGATCCTGAA GGAGCTGGAG GAGTCCTCGT TTAGGAAGAC GTTTGAGGAT TACCTGCACA 
     3241 ACGTGGTTTT CGTCCCCAGA AAAACCTCTT CAGGCACTGG TGCCGAGGAC CCTAGGCCAT 
     3301 CTCGGAAACG CAGGTCCCTT GGCGATGTTG GGAATGTGAC GGTGGCCGTG CCCACGGTGG 
     3361 CAGCTTTCCC CAACACTTCC TCGACCAGCG TGCCCACGAG TCCGGAGGAG CACAGGCCTT 
     3421 TTGAGAAGGT GGTGAACAAG GAGTCGCTGG TCATCTCCGG CTTGCGACAC TTCACGGGCT 
     3481 ATCGCATCGA GCTGCAGGCT TGCAACCAGG ACACCCCTGA GGAACGGTGC AGTGTGGCAG 
     3541 CCTACGTCAG TGCGAGGACC ATGCCTGAAG CCAAGGCTGA TGACATTGTT GGCCCTGTGA 
     3601 CGCATGAAAT CTTTGAGAAC AACGTCGTCC ACTTGATGTG GCAGGAGCCG AAGGAGCCCA 
     3661 ATGGTCTGAT CGTGCTGTAT GAAGTGAGTT ATCGGCGATA TGGTGATGAG GAGCTGCATC 
     3721 TCTGCGTCTC CCGCAAGCAC TTCGCTCTGG AACGGGGCTG CAGGCTGCGT GGGCTGTCAC 
     3781 CGGGGAACTA CAGCGTGCGA ATCCGGGCCA CCTCCCTTGC GGGCAACGGC TCTTGGACGG 
     3841 AACCCACCTA TTTCTACGTG ACAGACTATT TAGACGTCCC GTCAAATATT GCAAAAATTA 
     3901 TCATCGGCCC CCTCATCTTT GTCTTTCTCT TCAGTGTTGT GATTGGAAGT ATTTATCTAT 
     3961 TCCTGAGAAA GAGGCAGCCA GATGGGCCGC TGGGACCGCT TTACGCTTCT TCAAACCCTG 
     4021 AGTATCTCAG TGCCAGTGAT GTGTTTCCAT GCTCTGTGTA CGTGCCGGAC GAGTGGGAGG 
     4081 TGTCTCGAGA GAAGATCACC CTCCTTCGAG AGCTGGGGCA GGGCTCCTTC GGCATGGTGT 
     4141 ATGAGGGCAA TGCCAGGGAC ATCATCAAGG GTGAGGCAGA GACCCGCGTG GCGGTGAAGA 
     4201 CGGTCAACGA GTCAGCCAGT CTCCGAGAGC GGATTGAGTT CCTCAATGAG GCCTCGGTCA 
     4261 TGAAGGGCTT CACCTGCCAT CATGTGGTGC GCCTCCTGGG AGTGGTGTCC AAGGGCCAGC 
     4321 CCACGCTGGT GGTGATGGAG CTGATGGCTC ACGGAGACCT GAAGAGCTAC CTCCGTTCTC 
     4381 TGCGGCCAGA GGCTGAGAAT AATCCTGGCC GCCCTCCCCC TACCCTTCAA GAGATGATTC 
     4441 AGATGGCGGC AGAGATTGCT GACGGGATGG CCTACCTGAA CGCCAAGAAG TTTGTGCATC 
     4501 GGGACCTGGC AGCGAGAAAC TGCATGGTCG CCCATGATTT TACTGTCAAA ATTGGAGACT 
     4561 TTGGAATGAC CAGAGACATC TATGAAACGG ATTACTACCG GAAAGGGGGC AAGGGTCTGC 
     4621 TCCCTGTACG GTGGATGGCA CCGGAGTCCC TGAAGGATGG GGTCTTCACC ACTTCTTCTG 
     4681 ACATGTGGTC CTTTGGCGTG GTCCTTTGGG AAATCACCAG CTTGGCAGAA CAGCCTTACC 
     4741 AAGGCCTGTC TAATGAACAG GTGTTGAAAT TTGTCATGGA TGGAGGGTAT CTGGATCAAC 
     4801 CCGACAACTG TCCAGAGAGA GTCACTGACC TCATGCGCAT GTGCTGGCAA TTCAACCCCA 
     4861 AGATGAGGCC AACCTTCCTG GAGATTGTCA ACCTGCTCAA GGACGACCTG CACCCCAGCT 
     4921 TTCCAGAGGT GTCGTTCTTC CACAGCGAGG AGAACAAGGC TCCCGAGAGT GAGGAGCTGG 
     4981 AGATGGAGTT TGAGGACATG GAGAATGTGC CCCTGGACCG TTCCTCGCAC TGTCAGAGGG 
     5041 AGGAGGCGGG GGGCCGGGAT GGAGGGTCCT CGCTGGGTTT CAAGCGGAGC TACGAGGAAC 
     5101 ACATCCCTTA CACACACATG AACGGAGGCA AGAAAAACGG GCGGATTCTG ACCTTGCCTC 
     5161 GGTCCAATCC TTCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5221 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5281 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5341 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5401 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5461 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5521 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5581 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5641 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5701 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5761 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5821 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5881 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5941 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6001 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6061 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6121 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6181 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6241 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6301 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6361 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6421 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6481 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6541 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6601 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6661 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6721 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6781 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6841 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6901 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6961 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7021 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7081 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7141 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7201 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7261 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7321 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7381 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7441 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7501 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7561 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7621 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7681 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7741 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7801 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7861 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7921 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7981 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8041 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8101 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8161 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8221 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8281 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8341 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8401 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8461 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8521 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8581 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8641 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8701 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8761 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8821 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8881 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8941 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9001 ATTTAACAAA AATTTAACGC GAATTTT
//