LOCUS       RC215682                8895 bp    DNA     circular  SYN 
DEFINITION  CENPJ (Myc-DDK-tagged)-Human centromere protein J (CENPJ)
ACCESSION   NM_018451
VERSION     NM_018451.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5052..5143 
                     /label="Myc-DDK"  
     misc_feature    5173..5758 
                     /label="PolyA signal"  
     misc_feature    5230..5249 
                     /label="Restriction Enzyme"  
     misc_feature    6034..6706 
                     /label="ColE1"  
     misc_feature    7136..7930 
                     /label="Kan/Neo"  
     misc_feature    7965..8267 
                     /label="SV40 ori"  
     misc_feature    8353..8830 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5034..5040 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5034 
                     /label="CENPJ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCCTGATG CCAACCTCTT CAGAGTTAAA CAGTGGGCAG AACTTCCTAA 
     1081 CCCAGTGGGT GACCAATCCT TCTCGGGCTG GGGTCATATT AAATCGTGGA TTTCCTATTT 
     1141 TGGAAGCAGA CAAAGAGAAG CGAGCAGCTG TGGACATTTC TACCAGCTTT CCTATTAAAG 
     1201 GCACACATTT TTCTGATAGC TTCAGCTTTA TAAATGAAGA AGATTCACTT CTTGAAGAAC 
     1261 AGAAGTTGGA GTCAAACAAC CCTTACAAAC CACAGTCAGA TAAATCTGAA ACCCATACAG 
     1321 CCTTTCCTTG CATTAAAAAG GGACCACAGG TAGCGGCATG TCACAGTGCT CCTGGACACC 
     1381 AGGAAGAAAA CAAAAATGAC TTCATCCCAG ATCTTGCGAG TGAATTCAAA GAAGGGGCTT 
     1441 ATAAAGACCC ACTTTTTAAA AAACTTGAAC AGCTGAAAGA AGTACAACAG AAGAAGCAGG 
     1501 AACAATTGAA GAGGCAACAG TTGGAGCAAC TACAGAGACT CATGGAAGAA CAAGAGAAGC 
     1561 TGCTCACCAT GGTGTCTGGG CAGTGCACAC TTCCAGGTTT GAGTTTACTG CCTGATGATC 
     1621 AGAGCCAGAA GCACAGGTCT CCAGGAAATA CCACCACTGG AGAGAGAGCC ACATGCTGCT 
     1681 TCCCATCATA TGTCTACCCG GACCCAACCC AGGAAGAAAC ATACCCGTCC AACATTTTAT 
     1741 CCCATGAGCA AAGCAACTTC TGTAGAACTG CTCATGGAGA TTTTGTCTTA ACTTCAAAAC 
     1801 GTGCGTCTCC TAATTTATTT TCTGAGGCAC AGTATCAAGA AGCACCTGTG GAAAAAAATA 
     1861 ATTTAAAAGA AGAAAACCGT AACCATCCTA CAGGAGAAAG TATCTTATGT TGGGAGAAAG 
     1921 TGACAGAACA GATTCAGGAA GCAAATGATA AGAACTTACA AAAACATGAT GATTCCTCAG 
     1981 AAGTGGCTAA TATTGAAGAA AGGCCCATTA AAGCTGCTAT TGGAGAAAGG AAACAGACCT 
     2041 TTGAAGATTA CTTAGAAGAA CAAATTCAGT TGGAAGAGCA AGAACTGAAG CAAAAACAGC 
     2101 TGAAGGAAGC AGAAGGACCA TTGCCAATCA AAGCAAAACC AAAACAACCA TTTTTAAAAC 
     2161 GAGGAGAAGG TTTAGCTAGA TTTACTAATG CCAAATCTAA GTTTCAAAAA GGCAAAGAAA 
     2221 GTAAACTAGT GACTAACCAG AGCACTTCCG AGGACCAGCC GCTGTTTAAA ATGGATAGAC 
     2281 AGCAACTCCA GCGGAAAACC GCTCTTAAAA ATAAAGAGCT GTGTGCAGAC AACCCTATCC 
     2341 TTAAAAAGGA CAGTAAAGCT AGAACCAAGA GTGGTTCTGT CACCCTCAGT CAGAAGCCGA 
     2401 AAATGCTGAA GTGTAGTAAC AGGAAAAGTC TTTCTCCGTC AGGATTGAAA ATACAGACGG 
     2461 GGAAGAAATG TGATGGGCAG TTTAGAGACC AGATCAAATT TGAAAACAAA GTCACATCTA 
     2521 ATAATAAAGA AAATGTAACT GAGTGTCCAA AACCTTGCGA TACTGGCTGC ACAGGGTGGA 
     2581 ATAAGACACA AGGTAAAGAC AGACTTCCTC TTTCAACAGG GCCGGCCAGC CGGCTGGCTG 
     2641 CTAAGAGCCC CATAAGGGAG ACCATGAAAG AGTCTGAATC TTCTCTTGAC GTTTCTCTTC 
     2701 AGAAAAAGTT AGAGACTTGG GAACGAGAAA AGGAAAAGGA AAATTTGGAA TTAGATGAAT 
     2761 TTTTGTTTTT AGAACAAGCT GCTGATGAAA TATCATTTTC TAGTAATTCC TCATTTGTAC 
     2821 TGAAAATCTT AGAAAGGGAT CAACAGATCT GCAAAGGTCA CCGGATGTCT TCCACCCCTG 
     2881 TCAAAGCTGT GCCACAGAAG ACAAATCCGG CAGATCCCAT TAGTCATTGT AACCGCAGTG 
     2941 AGGATTTGGA CCACACTGCA CGTGAGAAGG AGAGTGAGTG TGAAGTCGCA CCCAAACAAC 
     3001 TTCATTCATT GTCCTCAGCT GATGAATTGA GGGAACAGCC TTGTAAAATC AGGAAAGCCG 
     3061 TCCAAAAGAG CACTTCTGAA AATCAGACTG AATGGAATGC ACGTGACGAT GAAGGTGTTC 
     3121 CAAATAGTGA CAGTAGCACT GACTCTGAGG AACAGCTTGA TGTTACCATA AAACCATCGA 
     3181 CTGAGGATAG AGAGAGGGGC ATCAGCAGCA GAGAGGATAG CCCACAAGTC TGTGATGATA 
     3241 AGGGGCCTTT TAAGGACACC AGGACCCAAG AAGATAAAAG GAGAGATGTT GATCTGGATT 
     3301 TGTCTGATAA AGATTACAGT AGCGATGAGT CTATCATGGA AAGCATAAAA CATAAAGTGT 
     3361 CTGAGCCCTC GAGATCCTCA TCCCTAAGTC TGAGTAAAAT GGACTTTGAT GATGAAAGAA 
     3421 CTTGGACTGA CCTTGAAGAG AATTTGTGTA ACCATGATGT TGTTCTTGGG AATGAATCCA 
     3481 CTTATGGGAC GCCGCAGACA TGCTACCCTA ATAATGAAAT AGGTATCCTG GACAAAACAA 
     3541 TAAAAAGGAA GATTGCACCA GTCAAGAGGG GAGAAGACTT GAGCAAGTCC AGGAGGAGCA 
     3601 GAAGTCCTCC TACATCGGAG CTGATGATGA AATTCTTTCC TTCTTTGAAA CCAAAACCAA 
     3661 AGTCAGATTC ACACTTGGGA AATGAACTCA AGTTAAACAT AAGTCAAGAC CAACCACCTG 
     3721 GTGACAATGC TCGATCCCAG GTTTTGAGAG AGAAAATTAT TGAATTGGAA ACAGAAATAG 
     3781 AAAAGTTTAA AGCTGAGAAC GCATCTTTAG CTAAACTTCG CATTGAACGA GAAAGTGCCT 
     3841 TGGAAAAACT CAGGAAAGAA ATTGCAGACT TCGAACAACA GAAAGCAAAA GAATTAGCTC 
     3901 GAATAGAAGA GTTTAAAAAG GAGGAGATGA GGAAGCTACA AAAGGAACGT AAAGTTTTTG 
     3961 AAAAGTATAC TACAGCTGCA AGAACTTTTC CAGATAAAAA GGAACGTGAA GAAATACAGA 
     4021 CTTTAAAACA GCAAATAGCA GATTTACGGG AAGATTTGAA AAGAAAGGAG ACCAAATGGT 
     4081 CAAGTACACA CAGCCGTCTC AGAAGCCAGA TACAAATGTT AGTCAGAGAG AACACAGACC 
     4141 TCCGGGAAGA AATAAAAGTG ATGGAAAGAT TCCGACTGGA TGCCTGGAAG AGAGCAGAAG 
     4201 CCATAGAGAG CAGCCTCGAG GTGGAGAAGA AGGACAAGCT TGCGAACACA TCTGTTCGAT 
     4261 TTCAAAACAG TCAGATTTCT TCAGGAACCC AGGTAGAAAA ATACAAGAAA AATTATCTTC 
     4321 CAATGCAAGG CAATCCACCT CGAAGATCCA AGTCTGCACC TCCTCGTGAT TTAGGCAATT 
     4381 TGGATAAGGG ACAAGCTGCC TCTCCCAGGG AGCCACTTGA ACCACTGAAC TTCCCAGATC 
     4441 CTGAATATAA AGAGGAGGAG GAAGACCAAG ACATACAGGG AGAAATCAGT CATCCTGATG 
     4501 GAAAGGTGGA AAAGGTTTAT AAGAATGGGT GCCGTGTTAT ACTGTTTCCC AATGGAACTC 
     4561 GAAAGGAAGT GAGTGCAGAT GGGAAGACCA TCACTGTCAC TTTCTTTAAT GGTGACGTGA 
     4621 AGCAGGTCAT GCCAGACCAA AGAGTGATCT ACTACTATGC AGCTGCCCAG ACCACTCACA 
     4681 CGACATACCC GGAGGGACTG GAAGTCTTAC ATTTCTCAAG TGGACAAATA GAAAAACATT 
     4741 ACCCAGATGG AAGAAAAGAA ATCACGTTTC CTGACCAGAC TGTTAAAAAC TTATTTCCTG 
     4801 ATGGACAAGA AGAAAGCATT TTCCCAGATG GTACAATTGT CAGAGTACAA CGTGATGGCA 
     4861 ACAAACTCAT AGAGTTTAAT AATGGCCAAA GAGAACTACA TACTGCCCAG TTCAAGAGAC 
     4921 GGGAATACCC AGATGGCACT GTTAAAACCG TATATGCAAA CGGTCATCAA GAAACGAAGT 
     4981 ACAGATCCGG TCGGATAAGA GTTAAGGACA AGGAGGGTAA TGTGCTAATG GACACGGAGC 
     5041 TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5101 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5161 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5221 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5281 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5341 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5401 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5461 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5521 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5581 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5641 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5701 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5761 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5821 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5881 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5941 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6001 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6061 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6121 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6181 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6241 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6301 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6361 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6421 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6481 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6541 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6601 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6661 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6721 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6781 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6841 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6901 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6961 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7021 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7081 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7141 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7201 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7261 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7321 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7381 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7441 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7501 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7561 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7621 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7681 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7741 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7801 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7861 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7921 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7981 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8041 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8101 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8221 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8281 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8341 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8461 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8521 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8581 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8641 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8701 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8761 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8821 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8881 TTTAACGCGA ATTTT
//