LOCUS       RC215638                8190 bp    DNA     circular  SYN 
DEFINITION  PALM2 (Myc-DDK-tagged)-Human PALM2-AKAP2 readthrough (PALM2-AKAP2), transcript variant 1
ACCESSION   NM_007203
VERSION     NM_007203.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4347..4438 
                     /label="Myc-DDK"  
     misc_feature    4468..5053 
                     /label="PolyA signal"  
     misc_feature    4525..4544 
                     /label="Restriction Enzyme"  
     misc_feature    5329..6001 
                     /label="ColE1"  
     misc_feature    6431..7225 
                     /label="Kan/Neo"  
     misc_feature    7260..7562 
                     /label="SV40 ori"  
     misc_feature    7648..8125 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4329..4335 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4329 
                     /label="PALM2-AKAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGGCG GAATTGCACA AGGAAAGGCT GCAAGCCATA GCAGAAAAAA 
     1081 GAAAGAGGCA GACTGAAATA GAAGGCAAGC GACAACAGCT TGACGAGCAG ATACTTCTGC 
     1141 TGCAGCATTC CAAGTCCAAA GTGCTTCGGG AGAAATGGCT GCTGCAGGGC ATACCCGCTG 
     1201 GAACTGCCGA AGAGGAGGAA GCCAGGAGGC GGCAGTCTGA AGAGGATGAG TTCAGAGTCA 
     1261 AGCAACTTGA AGATAACATT CAGAGGCTGG AGCAAGAAAT ACAAACGCTA GAAAGTGAAG 
     1321 AGTCCCAGAT ATCTGCCAAA GAGCAAATCA TCCTAGAGAA ACTGAAGGAA ACAGAAAAAT 
     1381 CCTTCAAGGA CTTTCAGAAG GGTTTCTCCA GTACGGATGG AGATGCAGTA AATTACATTT 
     1441 CCTCCCAGCT TCCCGACCTG CCAATCCTCT GTTCACGAAC AGCAGAACCA TCACCTGGGC 
     1501 AGGACGGGAC CAGCAGAGCG GCTGGAGTCG GGTGGGAGAA TGTGCTGCTA AAGGAAGGTG 
     1561 AGTCAGCCTC GAACGCCACA GAAACATCCG GCCCAGACAT GACTATCAAG AAGCCTCCCC 
     1621 AGCTTTCTGA GGATGATATC TGGCTAAAAA GCGAGGGAGA CAACTATAGT GCCACCCTCC 
     1681 TGGAGCCTGC TGCCAGCTCT CTTTCCCCAG ATCACAAAAA CATGGAAATT GAGGTGTCTG 
     1741 TTGCAGAATG TAAAAGTGTT CCTGGAATCA CCTCTACCCC ACATCCCATG GACCATCCCT 
     1801 CCGCTTTCTA TTCACCCCCG CATAATGGCC TCCTTACTGA TCACCACGAA TCCCTGGATA 
     1861 ATGATGTTGC CAGAGAGATC CGCTATCTAG ATGAGGTGCT AGAGGCCAAC TGCTGTGATT 
     1921 CTGCTGTGGA TGGAACGTAC AATGGAACAT CCTCCCCAGA GCCTGGTGCA GTGGTTCTGG 
     1981 TGGGCGGCCT AAGCCCCCCT GTCCACGAGG CGACCCAGCC AGAACCCACT GAAAGAACAG 
     2041 CTAGCCGGCA GGCACCTCCT CACATCGAGC TCAGTAATAG CAGCCCTGAC CCCATGGCAG 
     2101 AGGCAGAAAG AACAAATGGC CATTCCCCCA GCCAGCCTAG AGATGCGCTG GGGGACAGCC 
     2161 TGCAGGTGCC TGTCAGCCCC AGCTCCACAA CCAGCTCACG GTGTTCTTCC CGAGATGGAG 
     2221 AGTTCACTCT CACCACACTG AAAAAGGAGG CCAAGTTTGA GCTGCGTGCC TTCCATGAGG 
     2281 ACAAGAAGCC CTCCAAGCTC TTTGAGGATG ACGAGCATGA GAAAGAACAA TACTGCATTA 
     2341 GAAAAGTGAG GCCTTCAGAG GAGATGCTGG AGCTGGAAAA GGAGAGGAGA GAGCTCATCC 
     2401 GCAGCCAGGC CGTCAAGAAG AATCCTGGCA TTGCAGCAAA ATGGTGGAAT CCCCCGCAGG 
     2461 AAAAAACCAT CGAGGAGCAG CTGGACGAGG AACATCTGGA GTCGCACAAA AAGTACAAGG 
     2521 AGCGCAAAGA GAGAAGGGCA CAGCAGGAAC AGTTGCTGCT GCAGAAGCAG TTACAGCAGC 
     2581 AGCAGCAGCA GCCCCCATCG CAGCTCTGCA CAGCCCCTGC CTCTTCTCAT GAACGCGCAA 
     2641 GCATGATTGA CAAAGCAAAG GAGGACATTG TCACAGAGCA GATAGATTTC TCTGCTGCTC 
     2701 GCAAACAATT TCAGCTGATG GAGAATTCCA GGCAAGCGGT GGCCAAGGGC CAGAGTACAC 
     2761 CCAGGCTGTT CTCCATCAAG CCTTTCTACA GGCCTCTGGG GTCAGTCAAC TCAGACAAGC 
     2821 CACTGACTAA TCCGAGACCA CCTTCTGTCG GGGGACCTCC AGAAGACAGT GGTGCCTCAG 
     2881 CCGCCAAGGG ACAGAAATCC CCCGGTGCCC TGGAGACCCC ATCGGCAGCA GGAAGCCAGG 
     2941 GCAACACAGC CTCTCAGGGG AAGGAAGGGC CCTACAGCGA GCCTTCTAAA CGTGGGCCCT 
     3001 TATCTAAACT GTGGGCTGAG GATGGAGAAT TTACGAGCGC CCGGGCTGTC CTCACTGTGG 
     3061 TCAAGGATGA TGACCATGGG ATTTTGGATC AGTTCTCAAG ATCTGTCAAT GTCTCCTTGA 
     3121 CCCAAGAGGA GCTTGACTCT GGTCTGGACG AATTGTCGGT GAGGTCTCAG GATACCACAG 
     3181 TCCTGGAGAC CCTATCCAAT GATTTCAGCA TGGACAACAT CAGTGACAGC GGGGCATCCA 
     3241 ATGAGACAAC CAATGCCCTC CAGGAAAATT CACTGGCTGA TTTTTCTCTG CCCCAGACAC 
     3301 CACAAACTGA CAACCCCTCA GAGGGCCGAG GAGAAGGCGT CTCCAAGTCA TTTAGTGATC 
     3361 ATGGTTTCTA TTCCCCTTCC TCCACGCTGG GGGACTCTCC GTTGGTTGAT GACCCCTTGG 
     3421 AGTATCAGGC TGGCCTCCTG GTGCAGAATG CCATTCAACA AGCCATAGCC GAGCAGGTGG 
     3481 ATAAAGCTGT GTCCAAAACC AGCAGGGATG GAGCAGAGCA ACAGGGACCT GAAGCGACTG 
     3541 TAGAGGAAGC TGAAGCTGCG GCTTTCGGCT CAGAAAAGCC TCAGAGCATG TTTGAGCCAC 
     3601 CTCAGGTGTC TTCTCCTGTT CAAGAGAAAA GGGATGTATT ACCAAAGATT CTGCCTGCTG 
     3661 AAGACAGGGC GCTCAGGGAA AGGGGGCCCC CCCAGCCACT GCCAGCTGTG CAGCCCAGTG 
     3721 GCCCGATTAA CATGGAGGAG ACCAGGCCCG AAGGAAGCTA TTTCAGCAAG TACTCGGAGG 
     3781 CAGCTGAGCT GAGAAGCACA GCCTCCCTCC TGGCCACTCA AGAATCTGAC GTGATGGTTG 
     3841 GGCCTTTCAA GCTGAGGTCC AGGAAACAGC GGACTTTGTC CATGATTGAG GAAGAGATCC 
     3901 GAGCAGCTCA GGAAAGGGAA GAGGAGCTGA AGAGGCAGAG ACAAGTCTTG CAGAGTACGC 
     3961 AGAGCCCCAG GACAAAGAAT GCCCCATCAC TGCCCTCCAG AACATGCTAC AAAACTGCTC 
     4021 CAGGGAAAAT AGAGAAAGTC AAACCTCCTC CATCCCCCAC CACTGAAGGC CCCAGCTTGC 
     4081 AGCCTGACTT AGCCCCTGAA GAGGCTGCCG GAACCCAGCG GCCCAAGAAT CTGATGCAGA 
     4141 CCCTCATGGA AGACTATGAG ACACACAAAT CTAAAAGGCG CGAGAGAATG GATGATAGTA 
     4201 GTTACACTTC TAAGTTACTG TCTTGCAAGG TGACTTCCGA GGTCCTCGAG GCCACACGGG 
     4261 TTAATCGAAG AAAGAGCGCA CTGGCTTTGC GCTGGGAAGC AGGGATCTAT GCCAACCAGG 
     4321 AGGAAGAAGA CAACGAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4381 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4441 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4501 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4561 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4621 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4681 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4741 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4801 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4861 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4921 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4981 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5041 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5101 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5161 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5221 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5281 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5341 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5401 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5461 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5521 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5581 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5641 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5701 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5761 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5821 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5881 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5941 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6001 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6061 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6121 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6181 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6241 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6301 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6361 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6421 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6481 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6541 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6601 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6661 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6721 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6781 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6841 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6901 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6961 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7021 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7081 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7141 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7201 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7261 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7321 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7381 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7441 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7501 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7561 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7621 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7681 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7741 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7801 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7861 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7921 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7981 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8041 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8101 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8161 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//