LOCUS       RC215627                9513 bp    DNA     circular  SYN 
DEFINITION  TRPM3 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 3 (TRPM3), transcript variant 3
ACCESSION   NM_206944
VERSION     NM_206944.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5670..5761 
                     /label="Myc-DDK"  
     misc_feature    5791..6376 
                     /label="PolyA signal"  
     misc_feature    5848..5867 
                     /label="Restriction Enzyme"  
     misc_feature    6652..7324 
                     /label="ColE1"  
     misc_feature    7754..8548 
                     /label="Kan/Neo"  
     misc_feature    8583..8885 
                     /label="SV40 ori"  
     misc_feature    8971..9448 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5652..5658 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5652 
                     /label="TRPM3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGTGCGA GTATCTTTTG ATACAAAACC TGATCTCCTC TTACACCTGA 
     1081 TGACCAAGGA ATGGCAGTTG GAGCTTCCCA AGCTTCTCAT CTCTGTCCAT GGGGGCCTGC 
     1141 AGAACTTTGA ACTCCAGCCA AAACTCAAGC AAGTCTTTGG GAAAGGGCTC ATCAAAGCAG 
     1201 CAATGACAAC TGGAGCGTGG ATATTCACTG GAGGGGTTAA CACAGGTGTT ATTCGTCATG 
     1261 TTGGCGATGC CTTGAAGGAT CATGCCTCTA AGTCTCGAGG AAAGATATGC ACCATAGGTA 
     1321 TTGCCCCCTG GGGAATTGTG GAAAACCAGG AGGACCTCAT TGGAAGAGAT GTTGTCCGGC 
     1381 CATACCAGAC CATGTCCAAT CCCATGAGCA AGCTCACTGT TCTCAACAGC ATGCATTCCC 
     1441 ACTTCATTCT GGCTGACAAC GGGACCACTG GAAAATATGG AGCAGAGGTG AAACTTCGAA 
     1501 GACAACTGGA AAAGCATATT TCACTCCAGA AGATAAACAC AAGAATCGGT CAAGGTGTTC 
     1561 CTGTGGTGGC ACTCATAGTG GAAGGAGGAC CCAATGTGAT CTCGATTGTT TTGGAGTACC 
     1621 TTCGAGACAC CCCTCCCGTG CCAGTGGTTG TCTGTGATGG GAGTGGACGG GCATCGGACA 
     1681 TCCTGGCCTT TGGGCATAAA TACTCAGAAG AAGGCGGACT GATAAATGAA TCTTTGAGGG 
     1741 ACCAGCTGTT GGTGACTATA CAGAAGACTT TCACATACAC TCGAACCCAA GCTCAGCATC 
     1801 TGTTCATCAT CCTCATGGAG TGCATGAAGA AGAAGGAATT GATTACGGTA TTTCGGATGG 
     1861 GATCAGAAGG ACACCAGGAC ATTGATTTGG CTATCCTGAC AGCTTTACTC AAAGGAGCCA 
     1921 ATGCCTCGGC CCCAGACCAA CTGAGCTTAG CTTTAGCCTG GAACAGAGTC GACATCGCTC 
     1981 GCAGCCAGAT CTTTATTTAC GGGCAACAGT GGCCGGTGGG ATCTCTGGAG CAAGCCATGT 
     2041 TGGATGCCTT AGTTCTGGAC AGAGTGGATT TTGTGAAATT ACTCATAGAG AATGGAGTAA 
     2101 GCATGCACCG TTTTCTCACC ATCTCCAGAC TAGAGGAATT GTACAATACG AGACATGGGC 
     2161 CCTCAAATAC ATTGTACCAC TTGGTCAGGG ATGTCAAAAA GGGGAACCTG CCCCCAGACT 
     2221 ACAGAATCAG CCTGATTGAC ATCGGCCTGG TGATCGAGTA CCTGATGGGC GGGGCTTATC 
     2281 GCTGCAACTA CACGCGCAAG CGCTTCCGGA CCCTCTACCA CAACCTCTTC GGCCCCAAGA 
     2341 GGGATGATAT TCCCTTGAGG CGAGGAAGAA AGACAACCAA GAAACGTGAA GAAGAGGTGG 
     2401 ACATTGACTT GGATGATCCT GAGATCAACC ACTTCCCCTT CCCTTTCCAT GAGCTCATGG 
     2461 TGTGGGCTGT TCTCATGAAG CGGCAGAAGA TGGCCCTGTT CTTCTGGCAG CACGGTGAGG 
     2521 AGGCCATGGC CAAGGCCCTG GTGGCCTGCA AGCTCTGCAA AGCCATGGCT CATGAGGCCT 
     2581 CTGAGAACGA CATGGTTGAC GACATTTCCC AGGAGCTGAA TCACAATTCC AGAGACTTTG 
     2641 GCCAGCTGGC TGTGGAGCTC CTGGACCAGT CCTACAAGCA GGACGAACAG CTGGCCATGA 
     2701 AACTGCTGAC GTATGAGCTG AAGAACTGGA GCAACGCCAC GTGCCTGCAG CTTGCCGTGG 
     2761 CTGCCAAACA CCGCGACTTC ATCGCGCACA CGTGCAGCCA GATGCTGCTC ACCGACATGT 
     2821 GGATGGGCCG GCTCCGCATG CGCAAGAACT CAGGCCTCAA GGTAATTCTG GGAATTCTAC 
     2881 TTCCTCCTTC AATTCTCAGC TTGGAGTTCA AGAACAAAGA CGACATGCCC TATATGTCTC 
     2941 AGGCCCAGGA AATCCACCTC CAAGAGAAGG AGGCAGAAGA ACCAGAGAAG CCCACAAAGG 
     3001 AAAAAGAGGA AGAGGACATG GAGCTCACAG CAATGTTGGG ACGAAACAAC GGGGAGTCCT 
     3061 CCAGGAAGAA GGATGAAGAG GAAGTTCAGA GCAAGCACCG GTTAATCCCC CTCGGCAGAA 
     3121 AAATCTATGA ATTCTACAAT GCACCCATCG TGAAGTTCTG GTTCTACACA CTGGCGTATA 
     3181 TCGGATACCT GATGCTCTTC AACTATATCG TGTTAGTGAA GATGGAACGC TGGCCGTCCA 
     3241 CCCAGGAATG GATCGTAATC TCCTATATTT TCACCCTGGG AATAGAAAAG ATGAGAGAGA 
     3301 TTCTGATGTC AGAGCCAGGG AAGTTGCTAC AGAAAGTGAA GGTATGGCTG CAGGAGTACT 
     3361 GGAATGTCAC GGACCTCATC GCCATCCTTC TGTTTTCTGT CGGAATGATC CTTCGTCTCC 
     3421 AAGACCAGCC CTTCAGGAGT GACGGGAGGG TCATCTACTG CGTGAACATC ATTTACTGGT 
     3481 ATATCCGTCT CCTAGACATC TTCGGCGTGA ACAAGTATTT GGGCCCGTAT GTAATGATGA 
     3541 TTGGAAAAAT GATGATAGAC ATGATGTACT TTGTCATCAT TATGCTGGTG GTTCTGATGA 
     3601 GCTTTGGGGT CGCCAGGCAA GCCATCCTTT TTCCCAATGA GGAGCCATCA TGGAAACTGG 
     3661 CCAAGAACAT CTTCTACATG CCCTATTGGA TGATTTATGG GGAAGTGTTT GCGGACCAGA 
     3721 TAGACCCTCC CTGTGGACAG AATGAGACCC GAGAGGATGG TAAAATAATC CAGCTGCCTC 
     3781 CCTGCAAGAC AGGAGCTTGG ATCGTGCCGG CCATCATGGC CTGCTACCTC TTAGTGGCAA 
     3841 ACATCTTGCT GGTCAACCTC CTCATTGCTG TCTTTAACAA TACATTTTTT GAAGTAAAAT 
     3901 CGATATCCAA CCAAGTCTGG AAGTTTCAGA GGTATCAGCT CATCATGACT TTCCATGAAA 
     3961 GGCCAGTTCT GCCCCCACCA CTGATCATCT TCAGCCACAT GACCATGATA TTCCAGCACC 
     4021 TGTGCTGCCG ATGGAGGAAA CACGAGAGCG ACCCGGATGA AAGGGACTAC GGCCTGAAAC 
     4081 TCTTCATAAC CGATGATGAG CTCAAGAAAG TACATGACTT TGAAGAGCAA TGCATAGAAG 
     4141 AATACTTCAG AGAAAAGGAT GATCGGTTCA ACTCATCTAA TGATGAGAGG ATACGGGTGA 
     4201 CTTCAGAAAG GGTGGAGAAC ATGTCTATGC GGCTGGAGGA AGTCAACGAG AGAGAGCACT 
     4261 CCATGAAGGC TTCACTCCAG ACCGTGGACA TCCGGCTGGC GCAGCTGGAA GACCTTATCG 
     4321 GGCGCATGGC CACGGCCCTG GAGCGCCTGA CAGGTCTGGA GCGGGCCGAG TCCAACAAAA 
     4381 TCCGCTCGAG GACCTCGTCA GACTGCACGG ACGCCGCCTA CATTGTCCGT CAGAGCAGCT 
     4441 TCAACAGCCA GGAAGGGAAC ACCTTCAAGC TCCAAGAGAG TATAGACCCT GCAGGTGAGG 
     4501 AGACCATGTC CCCAACTTCT CCAACCTTAA TGCCCCGTAT GCGAAGCCAT TCTTTCTATT 
     4561 CGGTCAATAT GAAAGACAAA GGTGGTATAG AAAAGTTGGA AAGTATTTTT AAAGAAAGGT 
     4621 CCCTGAGCCT ACACCGGGCT ACTAGTTCCC ACTCTGTAGC AAAAGAACCC AAAGCTCCTG 
     4681 CAGCCCCTGC CAACACCTTG GCCATTGTTC CTGATTCCAG AAGACCATCA TCGTGTATAG 
     4741 ACATCTATGT CTCTGCTATG GATGAGCTCC ACTGTGATAT AGACCCTCTG GACAATTCCG 
     4801 TGAACATCCT TGGGCTGGGC GAGCCAAGCT TTTCAACTCC AGTACCTTCC ACAGCCCCTT 
     4861 CAAGTAGTGC CTATGCAACA CTTGCACCCA CAGACAGACC TCCAAGCCGG AGCATTGATT 
     4921 TTGAGGACAT CACCTCCATG GACACTAGAT CTTTTTCTTC AGACTACACC CACCTCCCAG 
     4981 AATGCCAAAA CCCCTGGGAC TCAGAGCCTC CGATGTACCA CACCATTGAG CGTTCCAAAA 
     5041 GTAGCCGCTA CCTAGCCACC ACACCCTTTC TTCTAGAAGA GGCTCCCATT GTGAAATCTC 
     5101 ATAGCTTTAT GTTTTCCCCC TCAAGGAGCT ATTATGCCAA CTTTGGGGTG CCTGTAAAAA 
     5161 CAGCAGAATA CACAAGTATT ACAGACTGTA TTGACACAAG GTGTGTCAAT GCCCCTCAAG 
     5221 CAATTGCGGA CAGAGCTGCC TTCCCTGGAG GTCTTGGAGA CAAAGTGGAG GACTTAACTT 
     5281 GCTGCCATCC AGAGCGAGAA GCAGAACTGA GTCACCCCAG CTCTGACAGT GAGGAGAATG 
     5341 AGGCCAAAGG CCGCAGAGCC ACCATTGCAA TATCCTCCCA GGAGGGTGAT AACTCAGAGA 
     5401 GAACCCTGTC CAACAACATC ACTGTTCCCA AGATAGAGCG CGCCAACAGC TACTCGGCAG 
     5461 AGGAGCCAAG TGCGCCATAT GCACACACCA GGAAGAGCTT CTCCATCAGT GACAAACTCG 
     5521 ACAGGCAGCG GAACACAGCA AGCCTGCGAA ATCCCTTCCA GAGAAGCAAG TCCTCCAAGC 
     5581 CGGAGGGCCG AGGGGACAGC CTGTCCATGA GGAGACTGTC CAGAACATCG GCTTTCCAAA 
     5641 GCTTTGAAAG CAAGCACAAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5701 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5761 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5821 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5881 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5941 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     6001 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     6061 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     6121 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6181 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6241 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6301 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6361 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6421 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6481 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6541 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6601 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6661 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6721 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6781 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6841 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6901 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6961 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     7021 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     7081 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     7141 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7201 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7261 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7321 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7381 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7441 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7501 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7561 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7621 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7681 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7741 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7801 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7861 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7921 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7981 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     8041 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     8101 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     8161 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8221 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8281 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8341 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8401 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8461 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8521 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8581 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8641 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8701 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8761 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8821 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8881 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8941 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9001 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     9061 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     9121 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9181 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9241 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9301 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9361 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9421 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9481 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//