LOCUS       RC215626                7632 bp    DNA     circular  SYN 
DEFINITION  CLCA4 (Myc-DDK-tagged)-Human chloride channel accessory 4 (CLCA4), transcript variant 1
ACCESSION   NM_012128
VERSION     NM_012128.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3789..3880 
                     /label="Myc-DDK"  
     misc_feature    3910..4495 
                     /label="PolyA signal"  
     misc_feature    3967..3986 
                     /label="Restriction Enzyme"  
     misc_feature    4771..5443 
                     /label="ColE1"  
     misc_feature    5873..6667 
                     /label="Kan/Neo"  
     misc_feature    6702..7004 
                     /label="SV40 ori"  
     misc_feature    7090..7567 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3771..3777 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3771 
                     /label="CLCA4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGTTATTC AGAGGTTTTG TTTTCCTCTT AGTTCTGTGC CTGCTGCACC 
     1081 AGTCAAATAC TTCCTTCATT AAGCTGAATA ATAATGGCTT TGAAGATATT GTCATTGTTA 
     1141 TAGATCCTAG TGTGCCAGAA GATGAAAAAA TAATTGAACA AATAGAGGAT ATGGTGACTA 
     1201 CAGCTTCTAC GTACCTGTTT GAAGCCACAG AAAAAAGATT TTTTTTCAAA AATGTATCTA 
     1261 TATTAATTCC TGAGAATTGG AAGGAAAATC CTCAGTACAA AAGGCCAAAA CATGAAAACC 
     1321 ATAAACATGC TGATGTTATA GTTGCACCAC CTACACTCCC AGGTAGAGAT GAACCATACA 
     1381 CCAAGCAGTT CACAGAATGT GGAGAGAAAG GCGAATACAT TCACTTCACC CCTGACCTTC 
     1441 TACTTGGAAA AAAACAAAAT GAATATGGAC CACCAGGCAA ACTGTTTGTC CATGAGTGGG 
     1501 CTCACCTCCG GTGGGGAGTG TTTGATGAGT ACAATGAAGA TCAGCCTTTC TACCGTGCTA 
     1561 AGTCAAAAAA AATCGAAGCA ACAAGGTGTT CCGCAGGTAT CTCTGGTAGA AATAGAGTTT 
     1621 ATAAGTGTCA AGGAGGCAGC TGTCTTAGTA GAGCATGCAG AATTGATTCT ACAACAAAAC 
     1681 TGTATGGAAA AGATTGTCAA TTCTTTCCTG ATAAAGTACA AACAGAAAAA GCATCCATAA 
     1741 TGTTTATGCA AAGTATTGAT TCTGTTGTTG AATTTTGTAA CGAAAAAACC CATAATCAAG 
     1801 AAGCTCCAAG CCTACAAAAC ATAAAGTGCA ATTTTAGAAG TACATGGGAG GTGATTAGCA 
     1861 ATTCTGAGGA TTTTAAAAAC ACCATACCCA TGGTGACACC ACCTCCTCCA CCTGTCTTCT 
     1921 CATTGCTGAA GATCAGTCAA AGAATTGTGT GCTTAGTTCT TGATAAGTCT GGAAGCATGG 
     1981 GGGGTAAGGA CCGCCTAAAT CGAATGAATC AAGCAGCAAA ACATTTCCTG CTGCAGACTG 
     2041 TTGAAAATGG ATCCTGGGTG GGGATGGTTC ACTTTGATAG TACTGCCACT ATTGTAAATA 
     2101 AGCTAATCCA AATAAAAAGC AGTGATGAAA GAAACACACT CATGGCAGGA TTACCTACAT 
     2161 ATCCTCTGGG AGGAACTTCC ATCTGCTCTG GAATTAAATA TGCATTTCAG GTGATTGGAG 
     2221 AGCTACATTC CCAACTCGAT GGATCCGAAG TACTGCTGCT GACTGATGGG GAGGATAACA 
     2281 CTGCAAGTTC TTGTATTGAT GAAGTGAAAC AAAGTGGGGC CATTGTTCAT TTTATTGCTT 
     2341 TGGGAAGAGC TGCTGATGAA GCAGTAATAG AGATGAGCAA GATAACAGGA GGAAGTCATT 
     2401 TTTATGTTTC AGATGAAGCT CAGAACAATG GCCTCATTGA TGCTTTTGGG GCTCTTACAT 
     2461 CAGGAAATAC TGATCTCTCC CAGAAGTCCC TTCAGCTCGA AAGTAAGGGA TTAACACTGA 
     2521 ATAGTAATGC CTGGATGAAC GACACTGTCA TAATTGATAG TACAGTGGGA AAGGACACGT 
     2581 TCTTTCTCAT CACATGGAAC AGTCTGCCTC CCAGTATTTC TCTCTGGGAT CCCAGTGGAA 
     2641 CAATAATGGA AAATTTCACA GTGGATGCAA CTTCCAAAAT GGCCTATCTC AGTATTCCAG 
     2701 GAACTGCAAA GGTGGGCACT TGGGCATACA ATCTTCAAGC CAAAGCGAAC CCAGAAACAT 
     2761 TAACTATTAC AGTAACTTCT CGAGCAGCAA ATTCTTCTGT GCCTCCAATC ACAGTGAATG 
     2821 CTAAAATGAA TAAGGACGTA AACAGTTTCC CCAGCCCAAT GATTGTTTAC GCAGAAATTC 
     2881 TACAAGGATA TGTACCTGTT CTTGGAGCCA ATGTGACTGC TTTCATTGAA TCACAGAATG 
     2941 GACATACAGA AGTTTTGGAA CTTTTGGATA ATGGTGCAGG CGCTGATTCT TTCAAGAATG 
     3001 ATGGAGTCTA CTCCAGGTAT TTTACAGCAT ATACAGAAAA TGGCAGATAT AGCTTAAAAG 
     3061 TTCGGGCTCA TGGAGGAGCA AACACTGCCA GGCTAAAATT ACGGCCTCCA CTGAATAGAG 
     3121 CCGCGTACAT ACCAGGCTGG GTAGTGAACG GGGAAATTGA AGCAAACCCG CCAAGACCTG 
     3181 AAATTGATGA GGATACTCAG ACCACCTTGG AGGATTTCAG CCGAACAGCA TCCGGAGGTG 
     3241 CATTTGTGGT ATCACAAGTC CCAAGCCTTC CCTTGCCTGA CCAATACCCA CCAAGTCAAA 
     3301 TCACAGACCT TGATGCCACA GTTCATGAGG ATAAGATTAT TCTTACATGG ACAGCACCAG 
     3361 GAGATAATTT TGATGTTGGA AAAGTTCAAC GTTATATCAT AAGAATAAGT GCAAGTATTC 
     3421 TTGATCTAAG AGACAGTTTT GATGATGCTC TTCAACTAAA TACTACTGAT CTGTCACCAA 
     3481 AGGAGGCCAA CTCCAAGGAA AGCTTTGCAT TTAAACCAGA AAATATCTCA GAAGAAAATG 
     3541 CAACCCACAT ATTTATTGCC ATTAAAAGTA TAGATAAAAG CAATTTGACA TCAAAAGTAT 
     3601 CCAACATTGC ACAAGTAACT TTGTTTATCC CTCAAGCAAA TCCTGATGAC ATTGATCCTA 
     3661 CACCTACTCC TACTCCTACT CCTGATAAAA GTCATAATTC TGGAGTTAAT ATTTCTACGC 
     3721 TGGTATTGTC TGTGATTGGG TCTGTTGTAA TTGTTAACTT TATTTTAAGT ACCACCATTA 
     3781 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3841 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3901 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3961 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4021 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4081 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4141 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4201 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4261 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4321 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4381 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4441 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4501 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4561 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4621 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4681 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4741 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4801 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4861 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4921 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4981 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5041 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5101 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5161 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5221 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5281 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5341 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5401 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5461 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5521 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5581 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5641 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5701 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5761 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5821 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5881 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5941 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6001 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6061 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6121 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6181 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6241 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6301 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6361 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6421 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6481 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6541 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6601 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6661 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6721 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6781 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6841 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6901 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6961 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7021 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7081 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7141 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7201 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7261 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7321 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7381 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7441 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7501 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7561 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7621 AACGCGAATT TT
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