LOCUS       RC215533                7293 bp    DNA     circular  SYN 
DEFINITION  FGFR3 (Myc-DDK-tagged)-Human fibroblast growth factor receptor 3 (FGFR3), transcript variant 1
ACCESSION   NM_000142
VERSION     NM_000142.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3448..3539 
                     /label="Myc-DDK"  
     misc_feature    3569..4154 
                     /label="PolyA signal"  
     misc_feature    3626..3645 
                     /label="Restriction Enzyme"  
     misc_feature    4430..5102 
                     /label="ColE1"  
     misc_feature    5532..6326 
                     /label="Kan/Neo"  
     misc_feature    6361..6663 
                     /label="SV40 ori"  
     misc_feature    6749..7226 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3438..3446 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3438 
                     /label="FGFR3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGCCCCT GCCTGCGCCC TCGCGCTCTG CGTGGCCGTG GCCATCGTGG 
     1081 CCGGCGCCTC CTCGGAGTCC TTGGGGACGG AGCAGCGCGT CGTGGGGCGA GCGGCAGAAG 
     1141 TCCCGGGCCC AGAGCCCGGC CAGCAGGAGC AGTTGGTCTT CGGCAGCGGG GATGCTGTGG 
     1201 AGCTGAGCTG TCCCCCGCCC GGGGGTGGTC CCATGGGGCC CACTGTCTGG GTCAAGGATG 
     1261 GCACAGGGCT GGTGCCCTCG GAGCGTGTCC TGGTGGGGCC CCAGCGGCTG CAGGTGCTGA 
     1321 ATGCCTCCCA CGAGGACTCC GGGGCCTACA GCTGCCGGCA GCGGCTCACG CAGCGCGTAC 
     1381 TGTGCCACTT CAGTGTGCGG GTGACAGACG CTCCATCCTC GGGAGATGAC GAAGACGGGG 
     1441 AGGACGAGGC TGAGGACACA GGTGTGGACA CAGGGGCCCC TTACTGGACA CGGCCCGAGC 
     1501 GGATGGACAA GAAGCTGCTG GCCGTGCCGG CCGCCAACAC CGTCCGCTTC CGCTGCCCAG 
     1561 CCGCTGGCAA CCCCACTCCC TCCATCTCCT GGCTGAAGAA CGGCAGGGAG TTCCGCGGCG 
     1621 AGCACCGCAT TGGAGGCATC AAGCTGCGGC ATCAGCAGTG GAGCCTGGTC ATGGAAAGCG 
     1681 TGGTGCCCTC GGACCGCGGC AACTACACCT GCGTCGTGGA GAACAAGTTT GGCAGCATCC 
     1741 GGCAGACGTA CACGCTGGAC GTGCTGGAGC GCTCCCCGCA CCGGCCCATC CTGCAGGCGG 
     1801 GGCTGCCGGC CAACCAGACG GCGGTGCTGG GCAGCGACGT GGAGTTCCAC TGCAAGGTGT 
     1861 ACAGTGACGC ACAGCCCCAC ATCCAGTGGC TCAAGCACGT GGAGGTGAAT GGCAGCAAGG 
     1921 TGGGCCCGGA CGGCACACCC TACGTTACCG TGCTCAAGAC GGCGGGCGCT AACACCACCG 
     1981 ACAAGGAGCT AGAGGTTCTC TCCTTGCACA ACGTCACCTT TGAGGACGCC GGGGAGTACA 
     2041 CCTGCCTGGC GGGCAATTCT ATTGGGTTTT CTCATCACTC TGCGTGGCTG GTGGTGCTGC 
     2101 CAGCCGAGGA GGAGCTGGTG GAGGCTGACG AGGCGGGCAG TGTGTATGCA GGCATCCTCA 
     2161 GCTACGGGGT GGGCTTCTTC CTGTTCATCC TGGTGGTGGC GGCTGTGACG CTCTGCCGCC 
     2221 TGCGCAGCCC CCCCAAGAAA GGCCTGGGCT CCCCCACCGT GCACAAGATC TCCCGCTTCC 
     2281 CGCTCAAGCG ACAGGTGTCC CTGGAGTCCA ACGCGTCCAT GAGCTCCAAC ACACCACTGG 
     2341 TGCGCATCGC AAGGCTGTCC TCAGGGGAGG GCCCCACGCT GGCCAATGTC TCCGAGCTCG 
     2401 AGCTGCCTGC CGACCCCAAA TGGGAGCTGT CTCGGGCCCG GCTGACCCTG GGCAAGCCCC 
     2461 TTGGGGAGGG CTGCTTCGGC CAGGTGGTCA TGGCGGAGGC CATCGGCATT GACAAGGACC 
     2521 GGGCCGCCAA GCCTGTCACC GTAGCCGTGA AGATGCTGAA AGACGATGCC ACTGACAAGG 
     2581 ACCTGTCGGA CCTGGTGTCT GAGATGGAGA TGATGAAGAT GATCGGCAAA CACAAAAACA 
     2641 TCATCAACCT GCTGGGCGCC TGCACGCAGG GCGGGCCCCT GTACGTGCTG GTGGAGTACG 
     2701 CGGCCAAGGG TAACCTGCGG GAGTTTCTGC GGGCGCGGCG GCCCCCGGGC CTGGACTACT 
     2761 CCTTCGACAC CTGCAAGCCG CCCGAGGAGC AGCTCACCTT CAAGGACCTG GTGTCCTGTG 
     2821 CCTACCAGGT GGCCCGGGGC ATGGAGTACT TGGCCTCCCA GAAGTGCATC CACAGGGACC 
     2881 TGGCTGCCCG CAATGTGCTG GTGACCGAGG ACAACGTGAT GAAGATCGCA GACTTCGGGC 
     2941 TGGCCCGGGA CGTGCACAAC CTCGACTACT ACAAGAAGAC AACCAACGGC CGGCTGCCCG 
     3001 TGAAGTGGAT GGCGCCTGAG GCCTTGTTTG ACCGAGTCTA CACTCACCAG AGTGACGTCT 
     3061 GGTCCTTTGG GGTCCTGCTC TGGGAGATCT TCACGCTGGG GGGCTCCCCG TACCCCGGCA 
     3121 TCCCTGTGGA GGAGCTCTTC AAGCTGCTGA AGGAGGGCCA CCGCATGGAC AAGCCCGCCA 
     3181 ACTGCACACA CGACCTGTAC ATGATCATGC GGGAGTGCTG GCATGCCGCG CCCTCCCAGA 
     3241 GGCCCACCTT CAAGCAGCTG GTGGAGGACC TGGACCGTGT CCTTACCGTG ACGTCCACCG 
     3301 ACGAGTACCT GGACCTGTCG GCGCCTTTCG AGCAGTACTC CCCGGGTGGC CAGGACACCC 
     3361 CCAGCTCCAG CTCCTCAGGG GACGACTCCG TGTTTGCCCA CGACCTGCTG CCCCCGGCCC 
     3421 CACCCAGCAG TGGGGGCTCG CGGACGACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3481 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3541 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3601 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3661 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3721 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3781 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3841 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3901 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3961 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4021 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4081 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4141 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4201 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4261 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4321 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4381 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4441 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4501 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4561 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4621 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4681 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4741 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4801 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4861 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4921 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4981 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5041 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5101 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5161 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5221 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5281 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5341 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5401 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5461 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5521 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5581 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5641 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5701 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5761 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5821 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5881 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5941 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6001 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6061 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6121 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6181 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6241 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6301 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6361 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6421 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6481 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6541 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6601 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6661 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6721 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6781 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6841 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6901 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6961 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7021 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7081 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7141 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7201 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7261 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//