LOCUS       RC215510                8397 bp    DNA     circular  SYN 
DEFINITION  LAMB3 (Myc-DDK-tagged)-Human laminin, beta 3 (LAMB3), transcript variant 1
ACCESSION   NM_000228
VERSION     NM_000228.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4554..4645 
                     /label="Myc-DDK"  
     misc_feature    4675..5260 
                     /label="PolyA signal"  
     misc_feature    4732..4751 
                     /label="Restriction Enzyme"  
     misc_feature    5536..6208 
                     /label="ColE1"  
     misc_feature    6638..7432 
                     /label="Kan/Neo"  
     misc_feature    7467..7769 
                     /label="SV40 ori"  
     misc_feature    7855..8332 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4536..4542 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4536 
                     /label="LAMB3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACCATTC TTCCTCTTGT GTTTTGCCCT GCCTGGCCTC CTGCATGCCC 
     1081 AACAAGCCTG CTCCCGTGGG GCCTGCTATC CACCTGTTGG GGACCTGCTT GTTGGGAGGA 
     1141 CCCGGTTTCT CCGAGCTTCA TCTACCTGTG GACTGACCAA GCCTGAGACC TACTGCACCC 
     1201 AGTATGGCGA GTGGCAGATG AAATGCTGCA AGTGTGACTC CAGGCAGCCT CACAACTACT 
     1261 ACAGTCACCG AGTAGAGAAT GTGGCTTCAT CCTCCGGCCC CATGCGCTGG TGGCAGTCAC 
     1321 AGAATGATGT GAACCCTGTC TCTCTGCAGC TGGACCTGGA CAGGAGATTC CAGCTTCAAG 
     1381 AAGTCATGAT GGAGTTCCAG GGGCCCATGC CCGCCGGCAT GCTGATTGAG CGCTCCTCAG 
     1441 ACTTCGGTAA GACCTGGCGA GTGTACCAGT ACCTGGCTGC CGACTGCACC TCCACCTTCC 
     1501 CTCGGGTCCG CCAGGGTCGG CCTCAGAGCT GGCAGGATGT TCGGTGCCAG TCCCTGCCTC 
     1561 AGAGGCCTAA TGCACGCCTA AATGGGGGGA AGGTCCAACT TAACCTTATG GATTTAGTGT 
     1621 CTGGGATTCC AGCAACTCAA AGTCAAAAAA TTCAAGAGGT GGGGGAGATC ACAAACTTGA 
     1681 GAGTCAATTT CACCAGGCTG GCCCCTGTGC CCCAAAGGGG CTACCACCCT CCCAGCGCCT 
     1741 ACTATGCTGT GTCCCAGCTC CGTCTGCAGG GGAGCTGCTT CTGTCACGGC CATGCTGATC 
     1801 GCTGCGCACC CAAGCCTGGG GCCTCTGCAG GCCCCTCCAC CGCTGTGCAG GTCCACGATG 
     1861 TCTGTGTCTG CCAGCACAAC ACTGCCGGCC CAAATTGTGA GCGCTGTGCA CCCTTCTACA 
     1921 ACAACCGGCC CTGGAGACCG GCGGAGGGCC AGGACGCCCA TGAATGCCAA AGGTGCGACT 
     1981 GCAATGGGCA CTCAGAGACA TGTCACTTTG ACCCCGCTGT GTTTGCCGCC AGCCAGGGGG 
     2041 CATATGGAGG TGTGTGTGAC AATTGCCGGG ACCACACCGA AGGCAAGAAC TGTGAGCGGT 
     2101 GTCAGCTGCA CTATTTCCGG AACCGGCGCC CGGGAGCTTC CATTCAGGAG ACCTGCATCT 
     2161 CCTGCGAGTG TGATCCGGAT GGGGCAGTGC CAGGGGCTCC CTGTGACCCA GTGACCGGGC 
     2221 AGTGTGTGTG CAAGGAGCAT GTGCAGGGAG AGCGCTGTGA CCTATGCAAG CCGGGCTTCA 
     2281 CTGGACTCAC CTACGCCAAC CCGCAGGGCT GCCACCGCTG TGACTGCAAC ATCCTGGGGT 
     2341 CCCGGAGGGA CATGCCGTGT GACGAGGAGA GTGGGCGCTG CCTTTGTCTG CCCAACGTGG 
     2401 TGGGTCCCAA ATGTGACCAG TGTGCTCCCT ACCACTGGAA GCTGGCCAGT GGCCAGGGCT 
     2461 GTGAACCGTG TGCCTGCGAC CCGCACAACT CCCTCAGCCC ACAGTGCAAC CAGTTCACAG 
     2521 GGCAGTGCCC CTGTCGGGAA GGCTTTGGTG GCCTGATGTG CAGCGCTGCA GCCATCCGCC 
     2581 AGTGTCCAGA CCGGACCTAT GGAGACGTGG CCACAGGATG CCGAGCCTGT GACTGTGATT 
     2641 TCCGGGGAAC AGAGGGCCCG GGCTGCGACA AGGCATCAGG CCGCTGCCTC TGCCGCCCTG 
     2701 GCTTGACCGG GCCCCGCTGT GACCAGTGCC AGCGAGGCTA CTGTAATCGC TACCCGGTGT 
     2761 GCGTGGCCTG CCACCCTTGC TTCCAGACCT ATGATGCGGA CCTCCGGGAG CAGGCCCTGC 
     2821 GCTTTGGTAG ACTCCGCAAT GCCACCGCCA GCCTGTGGTC AGGGCCTGGG CTGGAGGACC 
     2881 GTGGCCTGGC CTCCCGGATC CTAGATGCAA AGAGTAAGAT TGAGCAGATC CGAGCAGTTC 
     2941 TCAGCAGCCC CGCAGTCACA GAGCAGGAGG TGGCTCAGGT GGCCAGTGCC ATCCTCTCCC 
     3001 TCAGGCGAAC TCTCCAGGGC CTGCAGCTGG ATCTGCCCCT GGAGGAGGAG ACGTTGTCCC 
     3061 TTCCGAGAGA CCTGGAGAGT CTTGACAGAA GCTTCAATGG TCTCCTTACT ATGTATCAGA 
     3121 GGAAGAGGGA GCAGTTTGAA AAAATAAGCA GTGCTGATCC TTCAGGAGCC TTCCGGATGC 
     3181 TGAGCACAGC CTACGAGCAG TCAGCCCAGG CTGCTCAGCA GGTCTCCGAC AGCTCGCGCC 
     3241 TTTTGGACCA GCTCAGGGAC AGCCGGAGAG AGGCAGAGAG GCTGGTGCGG CAGGCGGGAG 
     3301 GAGGAGGAGG CACCGGCAGC CCCAAGCTTG TGGCCCTGAG GCTGGAGATG TCTTCGTTGC 
     3361 CTGACCTGAC ACCCACCTTC AACAAGCTCT GTGGCAACTC CAGGCAGATG GCTTGCACCC 
     3421 CAATATCATG CCCTGGTGAG CTATGTCCCC AAGACAATGG CACAGCCTGT GGCTCCCGCT 
     3481 GCAGGGGTGT CCTTCCCAGG GCCGGTGGGG CCTTCTTGAT GGCGGGGCAG GTGGCTGAGC 
     3541 AGCTGCGGGG CTTCAATGCC CAGCTCCAGC GGACCAGGCA GATGATTAGG GCAGCCGAGG 
     3601 AATCTGCCTC ACAGATTCAA TCCAGTGCCC AGCGCTTGGA GACCCAGGTG AGCGCCAGCC 
     3661 GCTCCCAGAT GGAGGAAGAT GTCAGACGCA CACGGCTCCT AATCCAGCAG GTCCGGGACT 
     3721 TCCTAACAGA CCCCGACACT GATGCAGCCA CTATCCAGGA GGTCAGCGAG GCCGTGCTGG 
     3781 CCCTGTGGCT GCCCACAGAC TCAGCTACTG TTCTGCAGAA GATGAATGAG ATCCAGGCCA 
     3841 TTGCAGCCAG GCTCCCCAAC GTGGACTTGG TGCTGTCCCA GACCAAGCAG GACATTGCGC 
     3901 GTGCCCGCCG GTTGCAGGCT GAGGCTGAGG AAGCCAGGAG CCGAGCCCAT GCAGTGGAGG 
     3961 GCCAGGTGGA AGATGTGGTT GGGAACCTGC GGCAGGGGAC AGTGGCACTG CAGGAAGCTC 
     4021 AGGACACCAT GCAAGGCACC AGCCGCTCCC TTCGGCTTAT CCAGGACAGG GTTGCTGAGG 
     4081 TTCAGCAGGT ACTGCGGCCA GCAGAAAAGC TGGTGACAAG CATGACCAAG CAGCTGGGTG 
     4141 ACTTCTGGAC ACGGATGGAG GAGCTCCGCC ACCAAGCCCG GCAGCAGGGG GCAGAGGCAG 
     4201 TCCAGGCCCA GCAGCTTGCG GAAGGTGCCA GCGAGCAGGC ATTGAGTGCC CAAGAGGGAT 
     4261 TTGAGAGAAT AAAACAAAAG TATGCTGAGT TGAAGGACCG GTTGGGTCAG AGTTCCATGC 
     4321 TGGGTGAGCA GGGTGCCCGG ATCCAGAGTG TGAAGACAGA GGCAGAGGAG CTGTTTGGGG 
     4381 AGACCATGGA GATGATGGAC AGGATGAAAG ACATGGAGTT GGAGCTGCTG CGGGGCAGCC 
     4441 AGGCCATCAT GCTGCGCTCA GCGGACCTGA CAGGACTGGA GAAGCGTGTG GAGCAGATCC 
     4501 GTGACCACAT CAATGGGCGC GTGCTCTACT ATGCCACCTG CAAGACGCGT ACGCGGCCGC 
     4561 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4621 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5341 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5401 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5461 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5521 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5581 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5641 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5701 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5761 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5821 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5881 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5941 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6001 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6061 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6121 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6181 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6241 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6301 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6361 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6421 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6481 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6541 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6601 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6661 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6721 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6781 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6841 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6901 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6961 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7021 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7081 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7141 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7201 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7261 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7321 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7381 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7441 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7501 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7561 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7621 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7801 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7861 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7921 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7981 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8041 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8101 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8161 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8221 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8281 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8341 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//