LOCUS       RC215484                8244 bp    DNA     circular  SYN 
DEFINITION  SLC4A5 (Myc-DDK-tagged)-Human solute carrier family 4, sodium bicarbonate cotransporter, member 5 (SLC4A5), transcript variant c
ACCESSION   NM_133478
VERSION     NM_133478.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4401..4492 
                     /label="Myc-DDK"  
     misc_feature    4522..5107 
                     /label="PolyA signal"  
     misc_feature    4579..4598 
                     /label="Restriction Enzyme"  
     misc_feature    5383..6055 
                     /label="ColE1"  
     misc_feature    6485..7279 
                     /label="Kan/Neo"  
     misc_feature    7314..7616 
                     /label="SV40 ori"  
     misc_feature    7702..8179 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4383..4389 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4383 
                     /label="SLC4A5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTGAAG GAGGAGAAGG CTGGGGTAGG AAAGCTGGAC CACACTAACC 
     1081 ACAGGAGGAG ATTTCCGGAT CAGAAAGAAT GCCCTCCTAT CCACATTGGG CTTCCAGTAC 
     1141 CCACTTACCC TCAAAGAAAA ACTGACCAGA AGGGACATCT TTCAGGCCTG CAAAAAGTCC 
     1201 ACTGGGGCCT GCGGCCAGAC CAGCCACAGC AGGAACTGAC TGGCCCAGGG AGTGGGGCAA 
     1261 GCAGCCAGGA CAGCAGCATG GATCTTATCA GCAGGACTCG GTCCCCAGCT GCTGAGCAGC 
     1321 TCCAGGACAT CCTGGGGGAG GAAGATGAGG CTCCCAACCC CACCCTCTTT ACAGAGATGG 
     1381 ATACTCTGCA GCATGACGGA GACCAGATGG AGTGGAAGGA GTCAGCCAGG TGGATAAAGT 
     1441 TTGAAGAAAA GGTAGAGGAA GGCGGCGAAC GCTGGAGCAA GCCCCACGTG TCCACACTAT 
     1501 CCCTGCACAG CCTCTTCGAG CTCCGTACCT GCCTGCAGAC GGGGACGGTG CTGCTGGATT 
     1561 TGGACAGTGG CTCCTTACCA CAGATCATAG ATGATGTCAT TGAGAAGCAG ATTGAGGATG 
     1621 GTCTCCTGCG GCCAGAGCTC CGGGAGAGGG TCAGTTACGT CCTCCTGAGG AGGCACCGCC 
     1681 ACCAAACCAA GAAGCCCATC CACCGCTCCT TAGCTGACAT TGGGAAGTCA GTCTCCACCA 
     1741 CAAATCGCAG TCCTGCCCGG AGCCCTGGTG CTGGCCCGAG TCTACACCAC TCCACGGAAG 
     1801 ACCTGCGGAT GCGGCAGAGT GCAAATTACG GACGTCTGTG TCATGCCCAG AGCAGAAGCA 
     1861 TGAATGACAT TTCTCTCACC CCAAACACAG ACCAGCGGAA AAACAAATTC ATGAAGAAGA 
     1921 TCCCCAAGGA CTCAGAAGCG TCCAACGTGC TCGTGGGCGA GGTGGACTTC CTAGACCAGC 
     1981 CATTCATCGC GTTCGTGCGC CTCATCCAGT CGGCCATGCT GGGAGGAGTG ACCGAGGTGC 
     2041 CTGTCCCCAC CAGATTTCTG TTTATACTAC TGGGACCTTC TGGGAGAGCA AAATCCTACA 
     2101 ATGAAATTGG CCGTGCCATT GCAACCCTCA TGGTAGATGA TCTCTTCAGT GACGTGGCCT 
     2161 ACAAAGCCCG CAATCGGGAA GATCTGATCG CAGGAATTGA TGAATTTCTG GATGAGGTCA 
     2221 TCGTCCTTCC TCCTGGAGAA TGGGACCCAA ATATCCGGAT TGAGCCCCCC AAGAAGGTGC 
     2281 CCTCTGCTGA CAAGAGGAAA TCTGTGTTCT CCCTAGCAGA GCTGGGCCAG ATGAATGGCT 
     2341 CTGTGGGAGG AGGCGGCGGA GCTCCTGGAG GAGGCAATGG AGGTGGTGGT GGTGGTGGCA 
     2401 GTGGCGGCGG GGCTGGCAGT GGCGGGGCCG GCGGAACAAG CAGCGGGGAT GATGGAGAGA 
     2461 TGCCAGCCAT GCATGAAATC GGGGAGGAAC TTATCTGGAC AGGAAGGTTC TTCGGTGGCC 
     2521 TGTGTCTGGA TATCAAGAGG AAGTTGCCCT GGTTCCCAAG TGACTTCTAT GATGGCTTCC 
     2581 ACATTCAGTC CATCTCTGCC ATCCTATTCA TCTACCTCGG CTGTATCACC AACGCGATCA 
     2641 CCTTTGGTGG GCTTCTGGGG GATGCCACCG ACAATTATCA GGGAGTGATG GAGAGCTTCC 
     2701 TGGGCACTGC CATGGCTGGC TCCTTGTTCT GCCTCTTCTC GGGACAGCCT CTCATCATTC 
     2761 TCAGCAGCAC GGGGCCCATC CTCATCTTTG AGAAGCTCCT CTTCGACTTC AGCAAAGGCA 
     2821 ATGGCCTGGA CTACATGGAG TTCCGCCTCT GGATTGGCCT ACACTCAGCT GTCCAGTGCC 
     2881 TTATCCTAGT GGCCACAGAT GCCAGCTTTA TCATCAAATA TATCACCCGC TTCACCGAGG 
     2941 AGGGCTTCTC CACCCTTATC AGCTTCATCT TCATCTACGA TGCCATCAAG AAGATGATCG 
     3001 GTGCCTTCAA GTACTACCCT ATCAATATGG ACTTCAAGCC AAACTTCATC ACTACCTACA 
     3061 AGTGCGAGTG TGTCGCCCCT GACACAGTGA ATACAACCGT GTTCAATGCT TCAGCCCCAT 
     3121 TGGCACCAGA CACCAACGCT TCTCTGTACA ACCTCCTTAA CCTCACAGCG TTGGACTGGT 
     3181 CCCTGCTGAG CAAGAAGGAG TGTCTGAGCT ACGGCGGGCG CCTGCTTGGG AATTCCTGCA 
     3241 AGTTTATCCC AGACCTGGCG CTCATGTCCT TCATCCTTTT CTTTGGGACA TACTCCATGA 
     3301 CCCTGACCCT GAAGAAGTTC AAATTCAGCC GCTATTTTCC TACCAAGGTC CGGGCCCTGG 
     3361 TGGCTGACTT TTCCATTGTT TTCTCCATCC TGATGTTCTG TGGAATCGAT GCCTGTTTTG 
     3421 GCCTAGAAAC TCCCAAGCTG CATGTGCCCA GTGTCATCAA GCCAACGCGG CCTGACCGAG 
     3481 GCTGGTTCGT GGCCCCCTTT GGGAAGAACC CGTGGTGGGT ATACCCAGCA AGCATCCTGC 
     3541 CCGCCCTGCT GGTGACCATC CTGATCTTCA TGGACCAGCA GATCACTGCC GTCATTGTCA 
     3601 ACCGGAAGGA GAACAAACTG AAGAAGGCTG CCGGCTACCA TCTGGACCTG TTCTGGGTGG 
     3661 GCATCCTCAT GGCTTTGTGC TCCTTTATGG GGCTCCCCTG GTACGTGGCT GCCACGGTCA 
     3721 TCTCCATCGC CCACATCGAC AGCCTCAAGA TGGAGACAGA GACCAGTGCC CCTGGGGAGC 
     3781 AGCCCCAGTT TCTGGGAGTC AGGGAACAGA GAGTAACCGG CATCATCGTC TTCATCCTGA 
     3841 CGGGAATCTC TGTCTTCCTG GCTCCCATCC TAAAGTGTAT CCCCCTGCCG GTGCTGTACG 
     3901 GAGTCTTCCT CTACATGGGC GTGGCCTCCC TGAATGGCAT CCAGTTCTGG GAACGCTGCA 
     3961 AGCTCTTCCT GATGCCAGCC AAGCACCAGC CGGACCATGC CTTCCTGCGG CACGTGCCGC 
     4021 TGCGCCGGAT CCACCTCTTC ACCCTGGTGC AGATCCTCTG CCTGGCGGTG CTCTGGATCC 
     4081 TCAAATCCAC GGTGGCTGCC ATCATCTTCC CGGTCATGAT CCTGGGCCTC ATCATCGTTC 
     4141 GAAGGCTTCT GGATTTCATC TTTTCCCAGC ACGACCTGGC CTGGATTGAC AACATCCTCC 
     4201 CAGAGAAGGA AAAAAAGGAG ACAGACAAGA AGAGGAAGAG AAAAAAAGGG GCCCACGAGG 
     4261 ACTGTGATGA GGAGCCCCAG TTCCCTCCTC CCTCGGTTAT AAAGATTCCC ATGGAAAGTG 
     4321 TCCAATCAGA TCCCCAAAAC GGTATCCACT GCATTGCCAG AAAAAGATCT TCCAGTTGGA 
     4381 GTTACTCACT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4441 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5221 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5281 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5341 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5401 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5461 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5521 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5581 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5641 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5701 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5761 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5821 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5881 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5941 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6001 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6061 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6121 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6181 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6241 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6301 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6361 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6421 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6481 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6541 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6601 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6661 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6721 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6781 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6841 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6901 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6961 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7021 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7081 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7141 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7201 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7261 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7321 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7381 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7441 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7681 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7741 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7801 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8221 TAACAAAAAT TTAACGCGAA TTTT
//