LOCUS       RC215441                8235 bp    DNA     circular  SYN 
DEFINITION  MAP3K9 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 9 (MAP3K9)
ACCESSION   NM_033141
VERSION     NM_033141.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4392..4483 
                     /label="Myc-DDK"  
     misc_feature    4513..5098 
                     /label="PolyA signal"  
     misc_feature    4570..4589 
                     /label="Restriction Enzyme"  
     misc_feature    5374..6046 
                     /label="ColE1"  
     misc_feature    6476..7270 
                     /label="Kan/Neo"  
     misc_feature    7305..7607 
                     /label="SV40 ori"  
     misc_feature    7693..8170 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4374..4380 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4374 
                     /label="MAP3K9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCTCC AGAGCGCTTC TCGGCTGCCT AGCGAGCGCC GCCGCTGCCG 
     1081 CCCCGCCGGG GGAGGATGGA GCAGGGGCCG GGGCCGAGGA GGAGGAGGAG GAGGAGGAGG 
     1141 AGGCGGCGGC GGCGGTGGGC CCCGGGGAGC TGGGCTGCGA CGCGCCGCTG CCCTACTGGA 
     1201 CGGCCGTGTT CGAGTACGAG GCGGCGGGCG AGGACGAGCT GACCCTGCGG CTGGGCGACG 
     1261 TGGTGGAGGT GCTGTCCAAG GACTCGCAGG TGTCCGGCGA CGAGGGCTGG TGGACCGGGC 
     1321 AGCTGAACCA GCGGGTGGGC ATCTTCCCCA GCAACTACGT GACCCCGCGC AGCGCCTTCT 
     1381 CCAGCCGCTG CCAGCCCGGC GGCGAGGACC CCAGTTGCTA CCCGCCCATT CAGTTGTTAG 
     1441 AAATTGATTT TGCGGAGCTC ACCTTGGAAG AGATTATTGG CATCGGGGGC TTTGGGAAGG 
     1501 TCTATCGTGC TTTCTGGATA GGGGATGAGG TTGCTGTGAA AGCAGCTCGC CACGACCCTG 
     1561 ATGAGGACAT CAGCCAGACC ATAGAGAATG TTCGCCAAGA GGCCAAGCTC TTCGCCATGC 
     1621 TGAAGCACCC CAACATCATT GCCCTAAGAG GGGTATGTCT GAAGGAGCCC AACCTCTGCT 
     1681 TGGTCATGGA GTTTGCTCGT GGAGGACCTT TGAATAGAGT GTTATCTGGG AAAAGGATTC 
     1741 CCCCAGACAT CCTGGTGAAT TGGGCTGTGC AGATTGCCAG AGGGATGAAC TACTTACATG 
     1801 ATGAGGCAAT TGTTCCCATC ATCCACCGCG ACCTTAAGTC CAGCAACATA TTGATCCTCC 
     1861 AGAAGGTGGA GAATGGAGAC CTGAGCAACA AGATTCTGAA GATCACTGAT TTTGGCCTGG 
     1921 CTCGGGAATG GCACCGAACC ACCAAGATGA GTGCGGCAGG GACGTATGCT TGGATGGCAC 
     1981 CCGAAGTCAT CCGGGCCTCC ATGTTTTCCA AAGGCAGTGA TGTGTGGAGC TATGGGGTGC 
     2041 TACTTTGGGA GTTGCTGACT GGTGAGGTGC CCTTTCGAGG CATTGATGGC TTAGCAGTCG 
     2101 CTTATGGAGT GGCCATGAAC AAACTCGCCC TTCCTATTCC TTCTACGTGC CCAGAACCTT 
     2161 TTGCCAAACT CATGGAAGAC TGCTGGAATC CTGATCCCCA CTCACGACCA TCTTTCACGA 
     2221 ATATCCTGGA CCAGCTAACC ACCATAGAGG AGTCTGGTTT CTTTGAAATG CCCAAGGACT 
     2281 CCTTCCACTG CCTGCAGGAC AACTGGAAAC ACGAGATTCA GGAGATGTTT GACCAACTCA 
     2341 GGGCCAAAGA AAAGGAACTT CGCACCTGGG AGGAGGAGCT GACGCGGGCT GCACTGCAGC 
     2401 AGAAGAACCA GGAGGAACTG CTGCGGCGTC GGGAGCAGGA GCTGGCCGAG CGGGAGATTG 
     2461 ACATCCTGGA ACGGGAGCTC AACATCATCA TCCACCAGCT GTGCCAGGAG AAGCCCCGGG 
     2521 TGAAGAAACG CAAGGGCAAG TTCAGGAAGA GCCGGCTGAA GCTCAAGGAT GGCAACCGCA 
     2581 TCAGCCTCCC TTCTGATTTC CAGCACAAGT TCACGGTGCA GGCCTCCCCT ACCATGGATA 
     2641 AAAGGAAGAG TCTTATCAAC AGCCGCTCCA GTCCTCCTGC AAGCCCCACC ATCATTCCTC 
     2701 GCCTTCGAGC CATCCAGTTG ACACCAGGTG AAAGCAGCAA AACCTGGGGC AGGAGCTCAG 
     2761 TCGTCCCAAA GGAGGAAGGG GAGGAGGAGG AGAAGAGGGC CCCAAAGAAG AAGGGACGGA 
     2821 CGTGGGGGCC AGGGACGCTT GGTCAGAAGG AGCTTGCCTC GGGAGATGAA GGATCCCCTC 
     2881 AGAGACGTGA GAAAGCTAAT GGTTTAAGTA CCCCATCAGA ATCTCCACAT TTCCACTTGG 
     2941 GCCTCAAGTC CCTGGTAGAT GGATATAAGC AGTGGTCGTC CAGTGCCCCC AACCTGGTGA 
     3001 AGGGCCCAAG GAGTAGCCCG GCCCTGCCAG GGTTCACCAG CCTTATGGAG ATGGCCTTGC 
     3061 TGGCAGCCAG TTGGGTGGTG CCCATCGACA TTGAAGAGGA TGAGGACAGT GAAGGCCCAG 
     3121 GGAGTGGAGA GAGTCGCCTA CAGCATTCAC CCAGCCAGTC CTACCTCTGT ATCCCATTCC 
     3181 CTCGTGGAGA GGATGGCGAT GGCCCCTCCA GTGATGGAAT CCATGAGGAG CCCACCCCAG 
     3241 TCAACTCGGC CACGAGTACC CCTCAGCTGA CGCCAACCAA CAGCCTCAAG CGGGGCGGTG 
     3301 CCCACCACCG CCGCTGCGAG GTGGCTCTGC TCGGCTGTGG GGCTGTTCTG GCAGCCACAG 
     3361 GCCTAGGGTT TGACTTGCTG GAAGCTGGCA AGTGCCAGCT GCTTCCCCTG GAGGAGCCTG 
     3421 AGCCACCAGC CCGGGAGGAG AAGAAAAGAC GGGAGGGTCT TTTTCAGAGG TCCAGCCGTC 
     3481 CTCGTCGGAG CACCAGCCCC CCATCCCGAA AGCTTTTCAA GAAGGAGGAG CCCATGCTGT 
     3541 TGCTAGGAGA CCCCTCTGCC TCCCTGACGC TGCTCTCCCT CTCCTCCATC TCCGAGTGCA 
     3601 ACTCCACACG CTCCCTGCTG CGCTCCGACA GCGATGAAAT TGTCGTGTAT GAGATGCCAG 
     3661 TCAGCCCAGT CGAGGCCCCT CCCCTGAGTC CATGTACCCA CAACCCCCTG GTCAATGTCC 
     3721 GAGTAGAGCG CTTCAAACGA GATCCTAACC AATCTCTGAC TCCCACCCAT GTCACCCTCA 
     3781 CCACCCCCTC GCAGCCCAGC AGTCACCGGC GGACTCCTTC TGATGGGGCC CTTAAGCCAG 
     3841 AGACTCTCCT AGCCAGCAGG AGCCCCTCCA GCAATGGGTT GAGCCCCAGT CCTGGAGCAG 
     3901 GAATGTTGAA AACCCCCAGT CCCAGCCGAG ACCCAGGTGA ATTCCCCCGT CTCCCTGACC 
     3961 CCAATGTGGT CTTCCCCCCA ACCCCAAGGC GCTGGAACAC TCAGCAGGAC TCTACCTTGG 
     4021 AGAGACCCAA GACTCTGGAG TTTCTGCCTC GGCCGCGTCC TTCTGCCAAC CGGCAACGGC 
     4081 TGGACCCTTG GTGGTTTGTG TCCCCCAGCC ATGCCCGCAG CACCTCCCCA GCCAACAGCT 
     4141 CCAGCACAGA GACGCCCAGC AACCTGGACT CCTGCTTTGC TAGCAGTAGC AGCACTGTAG 
     4201 AGGAGCGGCC TGGACTTCCA GCCCTGCTCC CGTTCCAGGC AGGGCCGCTG CCCCCGACTG 
     4261 AGCGGACGCT CCTGGACCTG GATGCAGAGG GGCAGAGTCA GGACAGCACC GTGCCGCTGT 
     4321 GCAGAGCGGA ACTGAACACA CACAGGCCTG CCCCTTATGA GATCCAGCAG GAGTTCTGGT 
     4381 CTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4441 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5161 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5221 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5281 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5341 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5401 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5461 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5521 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5581 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5641 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5701 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5761 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5821 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5881 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5941 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6001 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6061 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6121 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6181 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6241 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6301 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6361 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6421 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6481 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6541 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6601 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6661 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6721 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6781 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6841 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6901 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6961 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7021 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7081 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7141 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7201 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7261 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7321 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7381 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7441 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7681 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7741 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7801 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7861 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7921 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7981 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8041 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8101 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8161 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8221 TTTAACGCGA ATTTT
//