LOCUS       RC215404                6684 bp    DNA     circular  SYN 
DEFINITION  FBLN1 (Myc-DDK-tagged)-Human fibulin 1 (FBLN1), transcript variant B
ACCESSION   NM_006485
VERSION     NM_006485.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2841..2932 
                     /label="Myc-DDK"  
     misc_feature    2962..3547 
                     /label="PolyA signal"  
     misc_feature    3019..3038 
                     /label="Restriction Enzyme"  
     misc_feature    3823..4495 
                     /label="ColE1"  
     misc_feature    4925..5719 
                     /label="Kan/Neo"  
     misc_feature    5754..6056 
                     /label="SV40 ori"  
     misc_feature    6142..6619 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2823..2829 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2823 
                     /label="FBLN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCGCC GCGCCGTCGC GCCGGGTCCC GCTTCCGCTG CTGCTGCTCG 
     1081 GCGGCCTTGC GCTGCTGGCG GCCGGAGTGG ACGCGGATGT CCTCCTGGAG GCCTGCTGTG 
     1141 CGGACGGACA CCGGATGGCC ACTCATCAGA AGGACTGCTC GCTGCCATAT GCTACGGAAT 
     1201 CCAAAGAATG CAGGATGGTG CAGGAGCAGT GCTGCCACAG CCAGCTGGAG GAGCTGCACT 
     1261 GTGCCACGGG CATCAGCCTG GCCAACGAGC AGGACCGCTG TGCCACGCCC CACGGTGACA 
     1321 ACGCCAGCCT GGAGGCCACA TTTGTGAAGA GGTGCTGCCA TTGCTGTCTG CTGGGGAGGG 
     1381 CGGCCCAGGC CCAGGGCCAG AGCTGCGAGT ACAGCCTCAT GGTTGGCTAC CAGTGTGGAC 
     1441 AGGTCTTCCG GGCATGCTGT GTCAAGAGCC AGGAGACCGG AGATTTGGAT GTCGGGGGCC 
     1501 TCCAAGAAAC GGATAAGATC ATTGAGGTTG AGGAGGAACA AGAGGACCCA TATCTGAATG 
     1561 ACCGCTGCCG AGGAGGCGGG CCCTGCAAGC AGCAGTGCCG AGACACGGGT GACGAGGTGG 
     1621 TCTGCTCCTG CTTCGTGGGC TACCAGCTGC TGTCTGATGG TGTCTCCTGT GAAGATGTCA 
     1681 ATGAATGCAT CACGGGCAGC CACAGCTGCC GGCTTGGAGA ATCCTGCATC AACACAGTGG 
     1741 GCTCTTTCCG CTGCCAGCGG GACAGCAGCT GCGGGACTGG CTATGAGCTC ACAGAGGACA 
     1801 ATAGCTGCAA AGATATTGAC GAGTGTGAGA GTGGTATTCA TAACTGCCTC CCCGATTTTA 
     1861 TCTGTCAGAA TACTCTGGGA TCCTTCCGCT GCCGACCCAA GCTACAGTGC AAGAGTGGCT 
     1921 TTATACAAGA TGCTCTAGGC AACTGTATTG ATATCAATGA GTGTTTGAGT ATCAGTGCCC 
     1981 CGTGCCCTAT CGGGCATACA TGCATCAACA CAGAGGGCTC CTACACGTGC CAGAAGAACG 
     2041 TGCCCAACTG TGGCCGTGGC TACCATCTCA ACGAGGAGGG AACGCGCTGT GTTGATGTGG 
     2101 ACGAGTGCGC GCCACCTGCT GAGCCCTGTG GGAAGGGACA TCGCTGCGTG AACTCTCCCG 
     2161 GCAGTTTCCG CTGCGAATGC AAGACGGGTT ACTATTTTGA CGGCATCAGC AGGATGTGTG 
     2221 TCGATGTCAA CGAGTGCCAG CGCTACCCCG GGCGCCTGTG TGGCCACAAG TGCGAGAACA 
     2281 CGCTGGGCTC CTACCTCTGC AGCTGTTCCG TGGGCTTCCG GCTCTCTGTG GATGGCAGGT 
     2341 CATGTGAAGA CATCAATGAG TGCAGCAGCA GCCCCTGTAG CCAGGAGTGT GCCAACGTCT 
     2401 ACGGCTCCTA CCAGTGTTAC TGCCGGCGAG GCTACCAGCT CAGCGATGTG GATGGAGTCA 
     2461 CCTGTGAAGA CATCGACGAG TGCGCCCTGC CCACCGGGGG CCACATCTGC TCCTACCGCT 
     2521 GCATCAACAT CCCTGGAAGC TTCCAGTGCA GCTGCCCCTC GTCTGGCTAC AGGCTGGCCC 
     2581 CCAATGGCCG CAACTGCCAA GACATTGATG AGTGTGTGAC TGGCATCCAC AACTGCTCCA 
     2641 TCAACGAGAC CTGCTTCAAC ATCCAGGGCG GCTTCCGCTG CCTGGCCTTC GAGTGCCCTG 
     2701 AGAACTACCG CCGCTCCGCA GCCACGCAGA AATCCAAGAA GGGAAGGCAG AACACCCCAG 
     2761 CGGGATCAAG TAAAGAGGAC TGCAGGGTTC TTCCATGGAA GCAGGGGTTG GAGGATACCC 
     2821 ACCTTGATGC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     2881 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     2941 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3001 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3061 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3121 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3181 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3241 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3301 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3361 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3421 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3481 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3541 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3601 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3661 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3721 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     3781 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     3841 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     3901 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     3961 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4021 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4081 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4141 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4201 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4261 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4321 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4381 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4441 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4501 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4561 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4621 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4681 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4741 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     4801 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     4861 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     4921 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     4981 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5041 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5101 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5161 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5221 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5281 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5341 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5401 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5461 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5521 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5581 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5641 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5701 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     5761 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     5821 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     5881 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     5941 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6001 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6061 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6121 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6181 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6241 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6301 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6361 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6421 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6481 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6541 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6601 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6661 TAACAAAAAT TTAACGCGAA TTTT
//