LOCUS       RC215399                8505 bp    DNA     circular  SYN 
DEFINITION  RECQL4 (Myc-DDK-tagged)-Human RecQ protein-like 4 (RECQL4)
ACCESSION   NM_004260
VERSION     NM_004260.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4662..4753 
                     /label="Myc-DDK"  
     misc_feature    4783..5368 
                     /label="PolyA signal"  
     misc_feature    4840..4859 
                     /label="Restriction Enzyme"  
     misc_feature    5644..6316 
                     /label="ColE1"  
     misc_feature    6746..7540 
                     /label="Kan/Neo"  
     misc_feature    7575..7877 
                     /label="SV40 ori"  
     misc_feature    7963..8440 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4644..4650 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4644 
                     /label="RECQL4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGGCTG CGGGACGTGC GGGAGCGGCT GCAGGCGTGG GAGCGCGCGT 
     1081 TCCGACGGCA GCGCGGGCGG CGACCGAGCC AGGACGACGT GGAGGCGGCG CCGGAGGAGA 
     1141 CCCGCGCGCT CTACCGGGAA TACCGCACTC TGAAGCGTAC CACGGGCCAG GCCGGCGGCG 
     1201 GGCTCCGCAG CTCCGAGTCG CTCCCCGCGG CGGCCGAAGA GGCGCCAGAG CCCCGCTGCT 
     1261 GGGGGCCCCA TCTGAATCGG GCTGCGACCA AGAGTCCACA GTCTACGCCA GGGCGGAGCC 
     1321 GCCAGGGCTC GGTGCCGGAC TACGGGCAGC GGCTCAAGGC CAATCTGAAA GGCACCCTGC 
     1381 AGGCCGGACC AGCCCTGGGC CGCAGACCGT GGCCTCTAGG AAGAGCCTCA TCTAAGGCAT 
     1441 CCACCCCAAA GCCCCCAGGT ACAGGGCCTG TCCCCTCCTT TGCAGAAAAA GTCAGTGATG 
     1501 AGCCTCCACA GCTCCCTGAG CCCCAGCCAA GGCCAGGCCG GCTCCAGCAT CTGCAGGCAT 
     1561 CCCTGAGCCA GCGGCTGGGC TCCCTAGATC CTGGCTGGTT ACAGCGATGT CACAGTGAGG 
     1621 TCCCAGATTT TCTGGGGGCC CCCAAAGCCT GCAGGCCTGA TCTAGGCTCA GAGGAATCAC 
     1681 AACTTCTGAT CCCTGGTGAG TCGGCTGTCC TTGGTCCTGG TGCTGGCTCC CAGGGCCCAG 
     1741 AGGCTTCAGC CTTCCAAGAA GTCAGCATCC GTGTGGGGAG CCCCCAGCCC AGCAGCAGTG 
     1801 GAGGCGAGAA GCGGAGATGG AACGAGGAGC CCTGGGAGAG CCCCGCACAG GTCCAGCAGG 
     1861 AGAGCAGCCA AGCTGGACCC CCATCGGAGG GGGCTGGGGC TGTAGCAGTT GAGGAAGACC 
     1921 CTCCAGGGGA ACCTGTACAG GCACAGCCAC CTCAGCCCTG CAGCAGCCCA TCGAACCCCA 
     1981 GGTACCACGG ACTCAGCCCC TCCAGTCAAG CTAGGGCTGG GAAGGCTGAG GGCACAGCCC 
     2041 CCCTGCACAT CTTCCCTCGG CTGGCCCGCC ATGACAGGGG CAATTACGTA CGGCTCAACA 
     2101 TGAAGCAGAA ACACTACGTG CGGGGCCGGG CACTCCGTAG CAGGCTCCTC CGCAAGCAGG 
     2161 CATGGAAGCA GAAGTGGCGG AAGAAAGGGG AGTGTTTTGG GGGTGGTGGT GCCACAGTCA 
     2221 CAACCAAGGA GTCTTGTTTC CTGAACGAGC AGTTCGATCA CTGGGCAGCC CAGTGTCCCC 
     2281 GGCCAGCAAG TGAGGAAGAC ACAGATGCTG TTGGGCCTGA GCCACTGGTT CCTTCACCAC 
     2341 AACCTGTACC TGAGGTGCCC AGCCTGGACC CCACCGTGCT GCCACTCTAC TCCCTGGGGC 
     2401 CCTCAGGGCA GTTGGCAGAG ACGCCGGCTG AGGTGTTCCA GGCCCTGGAG CAGCTGGGGC 
     2461 ACCAAGCCTT TCGCCCTGGG CAGGAGCGTG CAGTCATGCG GATCCTGTCT GGCATCTCCA 
     2521 CGCTGCTGGT GCTGCCTACA GGTGCCGGCA AGTCCCTGTG CTACCAGCTC CCAGCGCTGC 
     2581 TCTACAGCCG GCGCAGCCCC TGCCTCACGT TGGTCGTCTC TCCCCTGCTG TCACTCATGG 
     2641 ATGACCAGGT GTCTGGCCTG CCACCGTGTC TCAAGGCGGC CTGCATACAC TCGGGCATGA 
     2701 CCAGGAAGCA ACGGGAATCT GTCCTGCAGA AGATTCGGGC AGCCCAGGTA CACGTGCTGA 
     2761 TGCTGACACC TGAGGCACTG GTGGGGGCGG GAGGCCTCCC TCCAGCCGCA CAGCTGCCTC 
     2821 CAGTTGCTTT TGCCTGCATT GATGAGGCCC ACTGCCTCTC CCAGTGGTCC CACAACTTCC 
     2881 GGCCCTGCTA CCTGCGCGTC TGCAAGGTGC TTCGGGAGCG CATGGGCGTG CACTGCTTCC 
     2941 TGGGCCTCAC AGCCACAGCC ACACGCCGCA CTGCCAGTGA CGTGGCACAG CACCTGGCTG 
     3001 TGGCTGAAGA GCCTGACCTC CACGGGCCAG CCCCAGTTCC CACCAACCTG CACCTTTCCG 
     3061 TGTCCATGGA CAGGGACACA GACCAGGCAC TGTTGACGCT GCTGCAAGGC AAACGTTTTC 
     3121 AAAACCTCGA TTCCATTATC ATTTACTGCA ACCGGCGCGA GGACACAGAG CGGATCGCTG 
     3181 CGCTCCTCCG AACCTGCCTG CACGCAGCCT GGGTCCCAGG GTCTGGAGGT CGTGCCCCCA 
     3241 AAACCACAGC CGAGGCCTAC CACGCGGGCA TGTGCAGCCG GGAACGGCGG CGGGTACAGC 
     3301 GAGCCTTCAT GCAGGGCCAG TTGCGGGTGG TGGTGGCCAC GGTGGCCTTT GGGATGGGGC 
     3361 TGGACCGGCC AGATGTGCGG GCTGTGCTGC ATCTGGGGCT GCCCCCAAGC TTCGAGAGCT 
     3421 ACGTGCAGGC CGTGGGCCGG GCCGGGCGTG ACGGGCAGCC TGCCCACTGC CACCTCTTCC 
     3481 TGCAGCCCCA GGGCGAAGAC CTGCGAGAGC TGCGCAGACA TGTGCACGCC GACAGCACGG 
     3541 ACTTCCTGGC TGTGAAGAGG CTGGTACAGC GCGTGTTCCC AGCCTGCACC TGCACCTGCA 
     3601 CCAGGCCGCC CTCGGAGCAG GAAGGGGCCG TGGGTGGGGA GAGGCCTGTG CCCAAGTACC 
     3661 CCCCTCAAGA GGCTGAGCAG CTTAGCCACC AAGCAGCCCC AGGACCCAGA AGGGTCTGCA 
     3721 TGGGCCATGA GCGGGCACTC CCAATACAGC TTACCGTACA GGCTTTGGAC ATGCCGGAGG 
     3781 AGGCCATCGA GACTTTGCTG TGCTACCTGG AGCTGCACCC ACACCACTGG CTGGAGCTGC 
     3841 TGGCGACCAC CTATACCCAT TGCCGTCTGA ACTGCCCTGG GGGCCCTGCC CAGCTCCAGG 
     3901 CCCTGGCCCA CAGGTGTCCC CCTTTGGCTG TGTGCTTGGC CCAGCAGCTG CCTGAGGACC 
     3961 CAGGGCAAGG CAGCAGCTCC GTGGAGTTTG ACATGGTCAA GCTGGTGGAC TCCATGGGCT 
     4021 GGGAGCTGGC CTCTGTGCGG CGGGCTCTCT GCCAGCTGCA GTGGGACCAC GAGCCCAGGA 
     4081 CAGGTGTGCG GCGTGGGACA GGGGTGCTTG TGGAGTTCAG TGAGCTGGCC TTCCACCTTC 
     4141 GCAGCCCGGG GGACTTGACC GCTGAGGAGA AGGACCAGAT ATGTGACTTC CTCTATGGCC 
     4201 GTGTGCAGGC CCGGGAGCGC CAGGCCCTGG CCCGTCTGCG CAGAACCTTC CAGGCCTTTC 
     4261 ACAGCGTAGC CTTCCCCAGC TGCGGGCCCT GCCTGGAGCA GCAGGATGAG GAGCGCAGCA 
     4321 CCAGGCTCAA GGACCTGCTC GGCCGCTACT TTGAGGAAGA GGAAGGGCAG GAGCCGGGAG 
     4381 GCATGGAGGA CGCACAGGGC CCCGAGCCAG GGCAGGCCAG ACTCCAGGAT TGGGAGGACC 
     4441 AGGTCCGCTG CGACATCCGC CAGTTCCTGT CCCTGAGGCC AGAGGAGAAG TTCTCCAGCA 
     4501 GGGCTGTGGC CCGCATCTTC CACGGCATCG GAAGCCCCTG CTACCCGGCC CAGGTGTACG 
     4561 GGCAGGACCG ACGCTTCTGG AGAAAATACC TGCACCTGAG CTTCCATGCC CTGGTGGGCC 
     4621 TGGCCACGGA AGAGCTCCTG CAGGTGGCCC GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4681 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4741 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4801 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4861 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4921 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4981 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5041 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5101 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5161 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5221 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5281 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5341 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5401 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5461 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5521 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5581 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5641 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5701 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5761 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5821 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5881 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5941 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6001 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6061 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6121 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6181 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6241 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6301 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6361 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6421 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6481 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6541 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6601 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6661 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6721 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6781 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6841 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6901 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6961 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7021 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7081 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7141 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7201 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7261 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7321 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7381 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7441 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7501 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7561 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7621 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7681 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7741 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7861 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7921 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7981 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8041 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8101 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8161 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8221 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8281 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8341 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8401 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8461 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//