LOCUS       RC215378                8898 bp    DNA     circular  SYN 
DEFINITION  CLSPN (Myc-DDK-tagged)-Human claspin (CLSPN), transcript variant 1
ACCESSION   NM_022111
VERSION     NM_022111.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5055..5146 
                     /label="Myc-DDK"  
     misc_feature    5176..5761 
                     /label="PolyA signal"  
     misc_feature    5233..5252 
                     /label="Restriction Enzyme"  
     misc_feature    6037..6709 
                     /label="ColE1"  
     misc_feature    7139..7933 
                     /label="Kan/Neo"  
     misc_feature    7968..8270 
                     /label="SV40 ori"  
     misc_feature    8356..8833 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5037..5043 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5037 
                     /label="CLSPN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGGCGAG GTGGGTTCTG AGGTTCACCT AGAAATCAAT GACCCAAACG 
     1081 TCATTTCACA AGAGGAAGCA GATAGTCCTT CAGATAGTGG ACAGGGCAGC TATGAAACAA 
     1141 TTGGACCCTT GAGTGAAGGA GATTCAGATG AAGAGATATT TGTAAGTAAG AAGTTGAAAA 
     1201 ACAGGAAGGT TCTACAAGAC AGTGATTCCG AAACAGAGGA CACAAATGCC TCTCCAGAGA 
     1261 AAACTACCTA TGACAGTGCC GAGGAGGAAA ATAAAGAGAA TTTATATGCT GGGAAAAATA 
     1321 CAAAAATCAA AAGGATTTAC AAAACTGTGG CAGACAGTGA TGAAAGTTAC ATGGAAAAGT 
     1381 CTTTGTATCA GGAAAATCTT GAAGCGCAAG TGAAACCTTG CTTAGAGCTG AGTCTTCAGT 
     1441 CTGGAAACTC TACAGACTTT ACCACTGACA GAAAGAGTTC CAAAAAGCAC ATACATGATA 
     1501 AAGAAGGAAC TGCAGGAAAA GCAAAAGTAA AATCAAAAAG AAGACTTGAG AAAGAGGAGA 
     1561 GAAAAATGGA AAAAATTAGA CAGCTAAAAA AGAAGGAAAC AAAAAACCAG GAAGATGATG 
     1621 TAGAACAGCC ATTTAATGAC AGTGGCTGTC TTCTTGTGGA TAAAGACCTT TTTGAAACTG 
     1681 GGTTGGAGGA TGAAAATAAC TCTCCATTGG AAGATGAAGA GTCATTAGAA TCAATAAGAG 
     1741 CAGCTGTAAA AAACAAAGTA AAAAAGCACA AGAAAAAAGA ACCATCTTTG GAGAGTGGGG 
     1801 TCCATTCATT TGAGGAAGGA AGTGAGTTAT CAAAAGGAAC CACGAGGAAG GAAAGAAAGG 
     1861 CAGCCAGATT AAGTAAAGAA GCATTAAAAC AACTGCATAG TGAGACTCAG CGCCTTATTC 
     1921 GAGAGTCTGC ACTGAACCTT CCATATCATA TGCCTGAGAA TAAAACCATT CATGATTTCT 
     1981 TCAAACGTAA ACCCCGGCCC ACTTGCCACG GAAATGCCAT GGCACTATTG AAGTCATCTA 
     2041 AATATCAGTC AAGCCATCAC AAAGAAATCA TAGACACTGC AAATACTACT GAAATGAACA 
     2101 GTGATCACCA TAGTAAAGGT TCTGAGCAGA CAACAGGTGC AGAAAATGAA GTGGAAACTA 
     2161 ATGCACTCCC TGTAGTTTCA AAGGAAACCC AGATCATTAC TGGATCAGAT GAGTCTTGCA 
     2221 GGAAGGATTT GGTAAAAAAT GAAGAGCTAG AAATTCAGGA GAAACAGAAG CAGAGTGACA 
     2281 TTAGACCTTC ACCTGGGGAC AGCTCAGTGT TGCAACAGGA ATCCAACTTC CTCGGGAACA 
     2341 ATCACAGTGA GGAATGTCAG GTTGGAGGGC TTGTAGCATT TGAACCTCAT GCCCTGGAGG 
     2401 GTGAAGGCCC CCAAAATCCA GAAGAAACAG ATGAGAAAGT GGAAGAGCCT GAGCAGCAAA 
     2461 ATAAATCATC AGCAGTTGGG CCACCTGAAA AAGTGAGACG GTTTACTCTG GATAGACTTA 
     2521 AGCAACTGGG AGTAGATGTT TCCATTAAAC CACGGCTAGG TGCTGATGAA GATTCCTTTG 
     2581 TGATACTTGA ACCTGAAACC AACAGAGAAC TGGAAGCCTT GAAGCAGCGT TTCTGGAAGC 
     2641 ATGCTAATCC AGCAGCCAAA CCCAGGGCTG GTCAGACAGT GAATGTGAAC GTCATAGTGA 
     2701 AAGACATGGG CACTGATGGA AAGGAAGAGC TAAAAGCAGA TGTGGTACCT GTGACTTTAG 
     2761 CACCTAAGAA GTTGGATGGA GCAAGCCACA CAAAACCAGG TGAAAAGCTT CAGGTGTTAA 
     2821 AAGCTAAACT GCAAGAAGCA ATGAAACTCC GAAGGTTTGA GGAGCGCCAG AAGCGCCAAG 
     2881 CACTGTTTAA ATTAGATAAT GAAGATGGGT TTGAGGAAGA GGAGGAGGAA GAGGAAGAAA 
     2941 TGACAGATGA GTCTGAGGAA GATGGAGAAG AGAAGGTAGA GAAAGAAGAG AAAGAGGAAG 
     3001 AACTAGAGGA AGAGGAGGAG AAAGAAGAGG AGGAGGAAGA AGAAGGAAAT CAGGAGACTG 
     3061 CAGAATTCCT TCTTAGTAGT GAAGAAATAG AAACAAAAGA TGAAAAAGAA ATGGATAAAG 
     3121 AAAATAATGA TGGCAGTAGT GAAATTGGCA AGGCAGTTGG CTTCCTCTCT GTTCCCAAGT 
     3181 CTCTCTCATC AGATTCTACT TTACTTCTGT TTAAGGACAG CTCTTCCAAG ATGGGTTACT 
     3241 TTCCTACTGA AGAAAAATCA GAAACAGATG AAAACTCAGG CAAGCAGCCT AGCAAACTGG 
     3301 ATGAGGATGA TTCATGTTCA TTGCTAACAA AGGAGAGCAG CCACAATAGC AGCTTTGAGC 
     3361 TGATTGGCTC CACGATTCCA TCCTATCAGC CTTGCAACAG ACAAACAGGC CGTGGGACCA 
     3421 GTTTTTTCCC TACAGCAGGA GGATTCAGAT CTCCTTCCCC TGGGCTATTT CGAGCCAGTT 
     3481 TGGTCAGCTC AGCTTCTAAG AGTTCAGGGA AACTGTCTGA GCCTTCACTT CCCATAGAGG 
     3541 ATTCCCAGGA TCTGTATAAC GCCTCCCCAG AGCCTAAGAC ACTTTTCCTA GGAGCAGGAG 
     3601 ACTTCCAGTT CTGTTTAGAA GATGACACTC AGAGCCAACT GTTGGATGCA GATGGGTTCT 
     3661 TAAATGTTAG AAACCACAGG AATCAGTACC AAGCTTTGAA GCCTCGATTG CCATTGGCCA 
     3721 GTATGGATGA GAATGCCATG GATGCCAACA TGGATGAGCT GTTGGATTTG TGTACTGGAA 
     3781 AGTTCACATC TCAGGCTGAA AAACATCTAC CCAGGAAGAG TGACAAGAAA GAGAACATGG 
     3841 AGGAACTTCT GAACCTTTGT TCAGGAAAAT TCACTTCTCA GGATGCCTCC ACTCCAGCCT 
     3901 CATCAGAGTT AAATAAACAG GAGAAGGAGA GCAGCATGGG TGATCCAATG GAAGAAGCAC 
     3961 TTGCTCTTTG CTCAGGCTCT TTTCCCACAG ACAAGGAAGA GGAAGACGAG GAGGAGGAAT 
     4021 TTGGAGACTT TCGGCTTGTT TCAAATGATA ATGAGTTTGA TAGTGATGAG GATGAACACA 
     4081 GTGACTCTGG TAATGATCTG GCACTGGAAG ACCATGAAGA TGATGATGAA GAAGAACTCC 
     4141 TGAAGCGATC TGAGAAGTTG AAAAGGCAAA TGAGGTTGAG GAAATACCTG GAGGATGAGG 
     4201 CAGAGGTGTC AGGAAGTGAT GTGGGAAGCG AAGATGAGTA TGATGGGGAA GAAATTGATG 
     4261 AATATGAAGA GGACGTAATT GATGAAGTAC TTCCTTCTGA TGAGGAACTG CAGAGTCAAA 
     4321 TCAAGAAAAT ACACATGAAA ACTATGTTGG ATGATGATAA GCGACAGCTA CGTTTATACC 
     4381 AAGAGAGGTA CCTTGCTGAT GGGGATCTGC ACAGCGATGG TCCTGGGCGA ATGAGGAAGT 
     4441 TTCGATGGAA AAACATAGAT GATGCTTCCC AGATGGACTT GTTCCACAGA GACTCTGATG 
     4501 ATGATCAGAC TGAAGAACAG CTTGATGAGT CAGAAGCCAG GTGGAGGAAG GAGCGAATTG 
     4561 AACGAGAGCA GTGGCTTCGG GACATGGCAC AGCAGGGGAA AATTACAGCT GAAGAAGAAG 
     4621 AAGAAATTGG GGAGGACAGT CAGTTTATGA TACTGGCCAA GAAAGTTACA GCCAAAGCAC 
     4681 TGCAGAAGAA TGCCAGTCGC CCTATGGTTA TTCAGGAATC AAAGTCTTTG CTCAGAAATC 
     4741 CTTTTGAAGC CATCAGACCA GGAAGTGCTC AACAGGTGAA GACAGGCTCA CTGCTAAACC 
     4801 AGCCCAAAGC TGTGCTTCAG AAACTGGCTG CTCTCTCTGA CCATAACCCC AGTGCTCCTC 
     4861 GAAATTCAAG AAACTTTGTC TTTCATACAC TTTCTCCTGT CAAGGCTGAG GCGGCAAAGG 
     4921 AATCGTCTAA GTCTCAGGTA AAGAAAAGGG GTCCATCTTT CATGACTTCT CCTTCACCTA 
     4981 AGCACCTCAA AACAGATGAT AGCACTTCAG GATTGACGCG AAGCATCTTC AAATATTTGG 
     5041 AGAGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5101 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5161 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5221 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5281 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5341 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5401 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5461 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5521 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5581 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5641 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5701 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5761 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5821 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5881 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5941 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6001 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6061 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6121 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6181 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6241 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6301 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6361 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6421 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6481 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6541 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6601 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6661 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6721 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6781 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6841 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6901 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6961 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7021 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7081 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7141 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7201 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7261 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7321 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7381 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7441 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7501 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7561 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7621 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7681 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7741 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7801 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7861 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7921 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7981 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8041 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8101 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8161 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8221 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8281 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8341 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8401 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8461 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8521 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8581 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8641 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8701 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8761 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8821 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8881 AAATTTAACG CGAATTTT
//