LOCUS       RC215278                8766 bp    DNA     circular  SYN 
DEFINITION  SYNJ1 (Myc-DDK-tagged)-Human synaptojanin 1 (SYNJ1), transcript variant 2
ACCESSION   NM_203446
VERSION     NM_203446.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4923..5014 
                     /label="Myc-DDK"  
     misc_feature    5044..5629 
                     /label="PolyA signal"  
     misc_feature    5101..5120 
                     /label="Restriction Enzyme"  
     misc_feature    5905..6577 
                     /label="ColE1"  
     misc_feature    7007..7801 
                     /label="Kan/Neo"  
     misc_feature    7836..8138 
                     /label="SV40 ori"  
     misc_feature    8224..8701 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4905..4911 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4905 
                     /label="SYNJ1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTTCAGT AAAGGATTCC GGATCTATCA CAAATTGGAT CCCCCACCTT 
     1081 TCAGCCTCAT AGTGGAAACT AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG 
     1141 TCGCTGTGCT CTCATCTGCA GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTACTGG 
     1201 ATGCATATGG ACTCTTAGGT GTTCTGCGGT TAAATCTTGG TGATACTATG TTACATTATC 
     1261 TGGTCCTAGT CACTGGATGT ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG 
     1321 TTACTTCCAC TGAGTTTATA TCACTGCGAA TCGATTCTTC AGATGAGGAT CGCATTTCAG 
     1381 AAGTGCGGAA AGTTTTGAAT TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCTGGCATCA 
     1441 GTTTAGATTT GAGTCTTAAT GCGCATCGTA GCATGCAAGA ACAGACAACT GATAATAGAT 
     1501 TTTTCTGGAA TCAGTCTTTG CATTTGCATC TCAAACACTA TGGCGTGAAT TGTGATGACT 
     1561 GGTTATTACG TCTTATGTGT GGAGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC 
     1621 AGGCGAAGGC TTGCCTCATT TCAAGATTAA GCTGTGAACG AGCTGGGACC AGGTTTAATG 
     1681 TCCGGGGAAC AAATGATGAT GGTCATGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT 
     1741 ACTTAGATGA CTCAGTTTCT TCCTTCATAC AAATCCGAGG ATCTGTTCCA TTGTTCTGGG 
     1801 AGCAACCAGG GTTGCAAGTG GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA 
     1861 ATGCACCTGC TTTTGACAGG CATTTTAGAA CACTTAAGAA CTTATATGGT AAACAAATAA 
     1921 TAGTAAATTT GCTTGGATCT AAGGAAGGTG AACATATGCT AAGTAAAGCT TTCCAGAGTC 
     1981 ATTTGAAAGC TTCTGAACAT GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA 
     2041 TGGTTAAGGG AGGAAAGGCA GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT 
     2101 TTCTAGATTA TGGATTTTTT TATTTCAATG GAAGTGAAGT TCAAAGATGC CAGAGTGGTA 
     2161 CAGTTCGAAC AAACTGCTTG GATTGTCTTG ATAGAACAAA TAGTGTGCAG GCATTTCTTG 
     2221 GCTTAGAGAT GCTAGCTAAA CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG 
     2281 TGACTCGCTT TCAAGAAGTT TTTCGGTCAA TGTGGTCCGT GAATGGTGAT TCAATCAGTA 
     2341 AGATATATGC AGGAACTGGA GCTCTTGAAG GGAAAGCGAA GTTAAAAGAT GGTGCTCGCT 
     2401 CTGTTACCCG AACAATTCAG AATAACTTCT TTGACAGCTC CAAGCAAGAG GCCATTGATG 
     2461 TTTTGCTACT GGGAAATACT CTGAATAGTG ATTTAGCTGA CAAAGCTCGA GCACTTTTAA 
     2521 CTACTGGAAG TTTGCGTGTT TCTGAGCAGA CATTACAGTC AGCATCTTCT AAAGTACTAA 
     2581 AGAGCATGTG TGAGAATTTC TACAAATATT CAAAGCCTAA GAAAATTCGA GTATGTGTCG 
     2641 GAACCTGGAA TGTGAATGGT GGGAAGCAAT TTCGCAGCAT AGCTTTTAAG AATCAGACAC 
     2701 TCACTGACTG GCTTCTTGAT GCACCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA 
     2761 GAAGTAAGCC AACTGATATA TTTGCAATTG GTTTTGAAGA AATGGTAGAA TTGAATGCTG 
     2821 GAAACATTGT GAGTGCAAGC ACAACAAATC AGAAGCTCTG GGCTGTAGAA CTTCAGAAGA 
     2881 CAATCTCCAG AGACAACAAG TATGTGCTGC TGGCTTCTGA ACAGTTGGTG GGCGTCTGTT 
     2941 TGTTTGTTTT TATCAGACCA CAGCATGCTC CTTTTATCAG GGATGTTGCA GTTGATACTG 
     3001 TGAAGACTGG AATGGGAGGT GCAACTGGAA ATAAGGGAGC AGTTGCAATC CGAATGCTCT 
     3061 TCCATACAAC CAGCCTTTGC TTCGTCTGTA GCCACTTTGC TGCAGGGCAG TCACAAGTCA 
     3121 AAGAAAGAAA TGAAGATTTT ATAGAAATAG CACGAAAATT GAGTTTTCCT ATGGGAAGGA 
     3181 TGCTATTTTC CCATGACTAT GTATTTTGGT GTGGTGATTT CAACTATCGA ATCGATCTCC 
     3241 CTAACGAAGA AGTTAAAGAG CTCATAAGAC AGCAAAATTG GGATTCTCTT ATAGCAGGAG 
     3301 ATCAACTTAT CAATCAGAAA AATGCTGGAC AGGTTTTTAG AGGATTTTTA GAAGGAAAGG 
     3361 TAACCTTTGC TCCGACATAT AAGTATGACT TGTTTTCTGA CGACTATGAC ACCAGTGAAA 
     3421 AGTGCCGCAC CCCTGCCTGG ACAGACCGTG TCCTTTGGAG AAGGAGGAAA TGGCCTTTTG 
     3481 ATAGATCAGC TGAAGATCTA GATCTTCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATTC 
     3541 TGTACACGTG GACTCCAGGC ACTTTGCTGC ACTATGGAAG AGCTGAGCTG AAGACTTCTG 
     3601 ACCACAGGCC TGTCGTTGCC CTGATTGATA TAGATATATT TGAAGTTGAA GCTGAAGAGA 
     3661 GGCAAAACAT TTATAAAGAA GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACAGTATTGG 
     3721 TCTCAATCAA AAGTTCTTTA CCAGAAAATA ATTTTTTTGA TGATGCCTTG ATTGATGAGC 
     3781 TTCTGCAGCA GTTTGCAAGT TTTGGTGAAG TTATACTTAT AAGATTTGTA GAAGATAAAA 
     3841 TGTGGGTTAC ATTTTTGGAG GGAAGCTCTG CCTTGAATGT TCTGAGCCTA AATGGTAAAG 
     3901 AGTTATTGAA TCGGACTATA ACTATTGCTT TAAAAAGTCC AGACTGGATC AAAAATTTGG 
     3961 AAGAAGAAAT GAGTTTAGAG AAAATTAGCA TTGCATTGCC ATCATCAACA AGCTCTACCC 
     4021 TGCTTGGTGA AGATGCAGAG GTTGCAGCAG ATTTTGATAT GGAAGGTGAT GTTGATGACT 
     4081 ATAGTGCTGA AGTGGAGGAA CTTCTTCCTC AGCATCTCCA GCCATCTTCA AGTTCCGGCC 
     4141 TTGGTACTTC CCCCAGCTCT TCACCCCGAA CTAGTCCCTG CCAGTCACCT ACAATATCAG 
     4201 AGGGTCCTGT ACCTTCCCTT CCCATCAGAC CAAGCCGAGC ACCGTCAAGA ACTCCTGGGC 
     4261 CTCCCAGTGC ACAGAGTTCT CCTATTGACG CGCAGCCAGC AACGCCGCTG CCGCAGAAAG 
     4321 ACCCCGCCCA GCCCTTGGAG CCCAAGCGGC CGCCGCCGCC CCGCCCGGTC GCCCCTCCCA 
     4381 CACGCCCGGC TCCCCCACAG AGACCTCCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCCA 
     4441 CTAGAAAGGA ATTTGGAGCA CCCAAAAGCC CTGGAACAAC AAGGAAAGAT AATATAGGAC 
     4501 GCAGTCAGCC TTCACCTCAA GCAGGACTTG CAGGCCCAGG ACCTGCTGGA TACAGTACAG 
     4561 CCAGACCGAC GATTCCTCCT CGTGCTGGAG TTATCAGTGC CCCACAGAGC CACGCGCGGG 
     4621 CATCTGCTGG AAGACTGACT CCTGAAAGCC AAAGCAAAAC ATCAGAAACG TCGAAAGGTT 
     4681 CAACTTTCCT TCCTGAACCA CTGAAGCCTC AGGCTGCTTT TCCTCCGCAG TCTTCTTTGC 
     4741 CCCCGCCTGC TCAAAGGTTG CAAGAGCCTC TTGTCCCTGT GGCAGCACCT ATGCCTCAGT 
     4801 CTGGCCCCCA GCCAAATTTG GAAACCCCAC CACAACCACC ACCTCGAAGC AGGTCATCCC 
     4861 ATAGCTTGCC TTCAGAAGCT TCCTCACAAC CGCAACAGGA GCAACCATCA GGGACGCGTA 
     4921 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4981 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5041 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5101 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5161 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5221 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5281 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5341 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5401 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5461 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5521 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5581 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5641 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5701 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5761 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5821 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5881 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5941 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6001 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6061 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6121 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6181 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6241 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6301 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6361 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6421 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6481 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6541 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6601 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6661 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6721 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6781 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6841 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6901 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6961 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7021 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7081 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7141 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7201 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7261 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7321 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7381 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7441 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7501 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7561 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7621 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7681 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7741 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7801 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7861 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7921 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7981 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8041 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8101 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8161 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8221 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8281 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8341 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8401 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8461 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8521 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8581 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8641 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8701 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8761 AATTTT
//