LOCUS       RC215274                8184 bp    DNA     circular  SYN 
DEFINITION  DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 156
ACCESSION   NM_006729
VERSION     NM_006729.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4341..4432 
                     /label="Myc-DDK"  
     misc_feature    4462..5047 
                     /label="PolyA signal"  
     misc_feature    4519..4538 
                     /label="Restriction Enzyme"  
     misc_feature    5323..5995 
                     /label="ColE1"  
     misc_feature    6425..7219 
                     /label="Kan/Neo"  
     misc_feature    7254..7556 
                     /label="SV40 ori"  
     misc_feature    7642..8119 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4323..4329 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4323 
                     /label="DIAPH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCAGCCC GGGGCGGCGG CGTCGGGAGC GGGAGGCGGC AGCGAGGAAC 
     1081 CCGGTGGGGG CCGGAGCAAC AAGCGGAGCG CGGGGAACCG GGCCGCCAAT GAAGAGGAAA 
     1141 CGAAAAACAA ACCCAAATTG AACATTCAAA TAAAAACTTT GGCAGATGAT GTGCGTGACC 
     1201 GAATTACAAG TTTTAGAAAA TCTACTGTCA AAAAAGAAAA ACCTCTTATT CAACATCCTA 
     1261 TTGATTCTCA AGTCGCGATG AGTGAGTTTC CTGCAGCTCA GCCATTATAT GATGAACGAT 
     1321 CTTTGAATTT GTCAGAAAAG GAAGTATTGG ATCTCTTTGA AAAAATGATG GAGGACATGA 
     1381 ACCTTAACGA AGAGAAAAAA GCTCCTTTAC GAAACAAAGA CTTTACCACC AAACGTGAGA 
     1441 TGGTTGTCCA GTATATTTCT GCCACTGCCA AATCTGGTGG GCTGAAAAAC AGCAAACATG 
     1501 AATGCACCCT GTCTTCACAA GAATATGTTC ATGAATTACG ATCGGGTATA TCAGATGAGA 
     1561 AACTTCTTAA TTGCCTAGAA TCCCTCAGGG TTTCTTTAAC CAGCAATCCG GTCAGCTGGG 
     1621 TTAACAACTT TGGCCATGAA GGTCTTGGAC TCTTATTGGA TGAGCTGGAA AAGCTTCTGG 
     1681 ACAAAAAACA GCAAGAAAAT ATTGACAAGA AGAATCAGTA TAAACTTATT CAATGCCTCA 
     1741 AAGCATTTAT GAATAATAAG TTTGGATTAC AAAGGATTCT AGGAGATGAA AGAAGTCTTT 
     1801 TACTATTGGC AAGAGCAATT GACCCCAAAC AACCCAACAT GATGACTGAA ATAGTAAAAA 
     1861 TACTTTCTGC TATTTGCATT GTTGGAGAAG AGAACATTCT AGATAAACTT TTAGGGGCTA 
     1921 TAACAACAGC AGCAGAAAGA AATAACAGGG AACGATTTTC ACCAATTGTG GAAGGTTTAG 
     1981 AAAATCAGGA AGCCTTGCAA TTACAGGTGG CCTGCATGCA GTTTATAAAT GCCCTTGTCA 
     2041 CTTCTCCTTA TGAGCTTGAT TTTCGAATAC ATTTAAGGAA TGAATTCCTC CGTTCAGGAC 
     2101 TAAAAACAAT GTTACCAGAT CTAAAAGAAA AAGAGAATGA TGAGCTTGAT ATTCAGTTGA 
     2161 AAGTATTTGA TGAAAACAAA GAAGATGACC TAACTGAATT ATCACACCGT CTCAATGACA 
     2221 TTCGAGCAGA AATGGATGAT ATGAATGAAG TCTACCATCT TCTATATAAT ATGCTGAAGG 
     2281 ACACTGCTGC TGAAAATTAC TTCTTATCTA TTCTACAACA TTTTTTGCTT ATCAGAAATG 
     2341 ATTATTATAT CAGGCCACAA TATTATAAAA TAATTGAGGA ATGTGTTTCA CAGATAGTGC 
     2401 TACACTGCAG TGGTATGGAT CCAGACTTCA AATACAGGCA AAGATTAGAC ATCGATTTAA 
     2461 CTCATCTGAT AGATTCTTGT GTGAACAAGG CGAAAGTTGA AGAAAGTGAA CAAAAAGCTG 
     2521 CAGAGTTTTC AAAGAAGTTC GATGAAGAAT TCACAGCTCG ACAGGAAGCT CAAGCAGAGC 
     2581 TTCAAAAAAG AGATGAGAAA ATCAAAGAAC TTGAAGCAGA AATCCAGCAA CTTCGAACCC 
     2641 AGGCACAAGT ACTCTCAAGT TCATCAGGAA TTCCAGGTCC TCCTGCAGCA CCTCCATTGC 
     2701 CAGGTGTAGG GCCGCCTCCA CCACCACCCG CGCCACCTCT ACCCGGAGGA GCTCCTCTTC 
     2761 CTCCTCCACC ACCTCCTTTA CCTGGAATGA TGGGGATACC ACCACCACCC CCACCACCAC 
     2821 TTTTATTTGG GGGACCTCCT CCACCACCAC CCCTTGGAGG AGTTCCTCCT CCCCCAGGAA 
     2881 TATCACTTAA TCTACCTTAT GGAATGAAGC AGAAAAAAAT GTATAAACCT GAAGTGTCCA 
     2941 TGAAGAGAAT CAATTGGTCA AAGATTGAAC CCACAGAATT ATCTGAGAAC TGTTTCTGGT 
     3001 TAAGAGTCAA AGAAGACAAG TTTGAGAATC CAGATCTCTT TGCCAAATTG GCATTGAATT 
     3061 TTGCTACTCA GATAAAAGTT CAAAAGAACG CAGAAGCATT AGAAGAAAAG AAGACTGGGC 
     3121 CTACAAAGAA GAAAGTGAAA GAACTGAGAA TTTTGGATCC CAAAACAGCT CAGAATCTGT 
     3181 CCATCTTTCT GGGATCATAT CGCATGCCAT ATGAAGACAT AAGAAACGTT ATTCTGGAGG 
     3241 TTAATGAAGA CATGCTGAGT GAGGCTTTAA TTCAGAACCT TGTGAAACAT CTTCCTGAGC 
     3301 AGAAGATACT CAACGAATTA GCAGAGCTTA AGAATGAATA TGATGACCTC TGTGAGCCTG 
     3361 AACAATTTGG AGTTGTGATG AGCTCTGTGA AAATGTTACA GCCTCGTCTC AGTAGTATCC 
     3421 TGTTCAAGCT CACATTTGAA GAACACATAA ACAACATCAA ACCAAGCATC ATAGCAGTAA 
     3481 CTCTTGCCTG TGAAGAACTG AAGAAAAGTG AAAGCTTTAA CAGACTTTTA GAGTTAGTTC 
     3541 TTCTTGTTGG AAACTACATG AACTCAGGCT CAAGAAATGC CCAGTCTTTG GGATTTAAGA 
     3601 TCAACTTCCT TTGTAAGATC AGAGATACTA AATCAGCGGA TCAAAAAACA ACCCTTTTGC 
     3661 ATTTTATTGC CGACATTTGT GAGGAAAAAT ATCGAGATAT CCTAAAATTT CCTGAAGAAC 
     3721 TGGAACACGT AGAAAGTGCA AGCAAAGTTT CAGCTCAAAT TCTCAAGAGC AACCTTGCAT 
     3781 CAATGGAACA ACAAATTGTT CATCTGGAAC GTGACATCAA GAAATTCCCC CAAGCAGAAA 
     3841 ATCAACACGA TAAGTTTGTG GAAAAGATGA CCAGCTTTAC AAAGACTGCC CGAGAACAGT 
     3901 ATGAAAAACT CTCCACCATG CACAACAACA TGATGAAGCT CTATGAGAAT CTTGGAGAAT 
     3961 ACTTCATTTT TGACTCAAAG ACAGTGAGCA TAGAAGAGTT CTTTGGTGAT CTCAACAACT 
     4021 TCCGAACTTT GTTTTTGGAA GCAGTGAGAG AAAACAATAA GAGAAGAGAA ATGGAAGAGA 
     4081 AGACCAGGAG GGCAAAACTT GCAAAAGAGA AAGCTGAACA AGAAAAGTTA GAACGCCAGA 
     4141 AGAAAAAGAA ACAACTCATT GATATAAACA AAGAGGGTGA TGAGACTGGT GTGATGGATA 
     4201 ATCTTCTAGA AGCCCTACAA TCAGGTGCAG CATTCAGAGA CCGTCGAAAG CGGATTCCAA 
     4261 GGAATCCAGA TAACAGACGA GTACCTTTGG AAAGGTCACG CTCTCGCCAC AATGGAGCTA 
     4321 TCTCATCTAA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4381 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4441 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4501 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4561 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4621 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4681 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4741 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4801 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4861 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4921 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4981 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5041 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5101 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5161 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5221 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5281 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5341 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5401 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5461 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5521 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5581 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5641 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5701 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5761 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5821 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5881 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5941 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6001 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6061 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6121 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6181 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6241 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6301 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6361 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6421 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6481 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6541 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6601 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6661 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6721 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6781 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6841 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6901 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6961 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7021 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7081 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7141 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7201 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7261 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7321 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7381 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7441 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7501 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7621 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7681 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7741 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7801 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7861 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7921 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7981 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8041 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8101 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8161 TAACAAAAAT TTAACGCGAA TTTT
//