LOCUS       RC215205                9078 bp    DNA     circular  SYN 
DEFINITION  NLRP1 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 1 (NLRP1), transcript variant 4
ACCESSION   NM_033007
VERSION     NM_033007.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5235..5326 
                     /label="Myc-DDK"  
     misc_feature    5356..5941 
                     /label="PolyA signal"  
     misc_feature    5413..5432 
                     /label="Restriction Enzyme"  
     misc_feature    6217..6889 
                     /label="ColE1"  
     misc_feature    7319..8113 
                     /label="Kan/Neo"  
     misc_feature    8148..8450 
                     /label="SV40 ori"  
     misc_feature    8536..9013 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5217..5223 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5217 
                     /label="NLRP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGCGGA GCCTGGGGCC GCCTGGCCTG TTACTTGGAG TTCCTGAAGA 
     1081 AGGAGGAGCT GAAGGAGTTC CAGCTTCTGC TCGCCAATAA AGCGCACTCC AGGAGCTCTT 
     1141 CGGGTGAGAC ACCCGCTCAG CCAGAGAAGA CGAGTGGCAT GGAGGTGGCC TCGTACCTGG 
     1201 TGGCTCAGTA TGGGGAGCAG CGGGCCTGGG ACCTAGCCCT CCATACCTGG GAGCAGATGG 
     1261 GGCTGAGGTC ACTGTGCGCC CAAGCCCAGG AAGGGGCAGG CCACTCTCCC TCATTCCCCT 
     1321 ACAGCCCAAG TGAACCCCAC CTGGGGTCTC CCAGCCAACC CACCTCCACC GCAGTGCTAA 
     1381 TGCCCTGGAT CCATGAATTG CCGGCGGGGT GCACCCAGGG CTCAGAGAGA AGGGTTTTGA 
     1441 GACAGCTGCC TGACACATCT GGACGCCGCT GGAGAGAAAT CTCTGCCTCA CTCCTCTACC 
     1501 AAGCTCTTCC AAGCTCCCCA GACCATGAGT CTCCAAGCCA GGAGTCACCC AACGCCCCCA 
     1561 CATCCACAGC AGTGCTGGGG AGCTGGGGAT CCCCACCTCA GCCCAGCCTA GCACCCAGAG 
     1621 AGCAGGAGGC TCCTGGGACC CAATGGCCTC TGGATGAAAC GTCAGGAATT TACTACACAG 
     1681 AAATCAGAGA AAGAGAGAGA GAGAAATCAG AGAAAGGCAG GCCCCCATGG GCAGCGGTGG 
     1741 TAGGAACGCC CCCACAGGCG CACACCAGCC TACAGCCCCA CCACCACCCA TGGGAGCCTT 
     1801 CTGTGAGAGA GAGCCTCTGT TCCACATGGC CCTGGAAAAA TGAGGATTTT AACCAAAAAT 
     1861 TCACACAGCT GCTACTTCTA CAAAGACCTC ACCCCAGAAG CCAAGATCCC CTGGTCAAGA 
     1921 GAAGCTGGCC TGATTATGTG GAGGAGAATC GAGGACATTT AATTGAGATC AGAGACTTAT 
     1981 TTGGCCCAGG CCTGGATACC CAAGAACCTC GCATAGTCAT ACTGCAGGGG GCTGCTGGAA 
     2041 TTGGGAAGTC AACACTGGCC AGGCAGGTGA AGGAAGCCTG GGGGAGAGGC CAGCTGTATG 
     2101 GGGACCGCTT CCAGCATGTC TTCTACTTCA GCTGCAGAGA GCTGGCCCAG TCCAAGGTGG 
     2161 TGAGTCTCGC TGAGCTCATC GGAAAAGATG GGACAGCCAC TCCGGCTCCC ATTAGACAGA 
     2221 TCCTGTCTAG GCCAGAGCGG CTGCTCTTCA TCCTCGATGG TGTAGATGAG CCAGGATGGG 
     2281 TCTTGCAGGA GCCGAGTTCT GAGCTCTGTC TGCACTGGAG CCAGCCACAG CCGGCGGATG 
     2341 CACTGCTGGG CAGTTTGCTG GGGAAAACTA TACTTCCCGA GGCATCCTTC CTGATCACGG 
     2401 CTCGGACCAC AGCTCTGCAG AACCTCATTC CTTCTTTGGA GCAGGCACGT TGGGTAGAGG 
     2461 TCCTGGGGTT CTCTGAGTCC AGCAGGAAGG AATATTTCTA CAGATATTTC ACAGATGAAA 
     2521 GGCAAGCAAT TAGAGCCTTT AGGTTGGTCA AATCAAACAA AGAGCTCTGG GCCCTGTGTC 
     2581 TTGTGCCCTG GGTGTCCTGG CTGGCCTGCA CTTGCCTGAT GCAGCAGATG AAGCGGAAGG 
     2641 AAAAACTCAC ACTGACTTCC AAGACCACCA CAACCCTCTG TCTACATTAC CTTGCCCAGG 
     2701 CTCTCCAAGC TCAGCCATTG GGACCCCAGC TCAGAGACCT CTGCTCTCTG GCTGCTGAGG 
     2761 GCATCTGGCA AAAAAAGACC CTTTTCAGTC CAGATGACCT CAGGAAGCAT GGGTTAGATG 
     2821 GGGCCATCAT CTCCACCTTC TTGAAGATGG GTATTCTTCA AGAGCACCCC ATCCCTCTGA 
     2881 GCTACAGCTT CATTCACCTC TGTTTCCAAG AGTTCTTTGC AGCAATGTCC TATGTCTTGG 
     2941 AGGATGAGAA GGGGAGAGGT AAACATTCTA ATTGCATCAT AGATTTGGAA AAGACGCTAG 
     3001 AAGCATATGG AATACATGGC CTGTTTGGGG CATCAACCAC ACGTTTCCTA TTGGGCCTGT 
     3061 TAAGTGATGA GGGGGAGAGA GAGATGGAGA ACATCTTTCA CTGCCGGCTG TCTCAGGGGA 
     3121 GGAACCTGAT GCAGTGGGTC CCGTCCCTGC AGCTGCTGCT GCAGCCACAC TCTCTGGAGT 
     3181 CCCTCCACTG CTTGTACGAG ACTCGGAACA AAACGTTCCT GACACAAGTG ATGGCCCATT 
     3241 TCGAAGAAAT GGGCATGTGT GTAGAAACAG ACATGGAGCT CTTAGTGTGC ACTTTCTGCA 
     3301 TTAAATTCAG CCGCCACGTG AAGAAGCTTC AGCTGATTGA GGGCAGGCAG CACAGATCAA 
     3361 CATGGAGCCC CACCATGGTA GTCCTGTTCA GGTGGGTCCC AGTCACAGAT GCCTATTGGC 
     3421 AGATTCTCTT CTCCGTCCTC AAGGTCACCA GAAACCTGAA GGAGCTGGAC CTAAGTGGAA 
     3481 ACTCGCTGAG CCACTCTGCA GTGAAGAGTC TTTGTAAGAC CCTGAGACGC CCTCGCTGCC 
     3541 TCCTGGAGAC CCTGCGGTTG GCTGGCTGTG GCCTCACAGC TGAGGACTGC AAGGACCTTG 
     3601 CCTTTGGGCT GAGAGCCAAC CAGACCCTGA CCGAGCTGGA CCTGAGCTTC AATGTGCTCA 
     3661 CGGATGCTGG AGCCAAACAC CTTTGCCAGA GACTGAGACA GCCGAGCTGC AAGCTACAGC 
     3721 GACTGCAGCT GGTCAGCTGT GGCCTCACGT CTGACTGCTG CCAGGACCTG GCCTCTGTGC 
     3781 TTAGTGCCAG CCCCAGCCTG AAGGAGCTAG ACCTGCAGCA GAACAACCTG GATGACGTTG 
     3841 GCGTGCGACT GCTCTGTGAG GGGCTCAGGC ATCCTGCCTG CAAACTCATA CGCCTGGGGA 
     3901 AACCAAGTGT GATGACCCCT ACTGAGGGCC TGGATACGGG AGAGATGAGT AATAGCACAT 
     3961 CCTCACTCAA GCGGCAGAGA CTCGGATCAG AGAGGGCGGC TTCCCATGTT GCTCAGGCTA 
     4021 ATCTCAAACT CCTGGACGTG AGCAAGATCT TCCCAATTGC TGAGATTGCA GAGGAAAGCT 
     4081 CCCCAGAGGT AGTACCGGTG GAACTCTTGT GCGTGCCTTC TCCTGCCTCT CAAGGGGACC 
     4141 TGCATACGAA GCCTTTGGGG ACTGACGATG ACTTCTGGGG CCCCACGGGG CCTGTGGCTA 
     4201 CTGAGGTAGT TGACAAAGAA AAGAACTTGT ACCGAGTTCA CTTCCCTGTA GCTGGCTCCT 
     4261 ACCGCTGGCC CAACACGGGT CTCTGCTTTG TGATGAGAGA AGCGGTGACC GTTGAGATTG 
     4321 AATTCTGTGT GTGGGACCAG TTCCTGGGTG AGATCAACCC ACAGCACAGC TGGATGGTGG 
     4381 CAGGGCCTCT GCTGGACATC AAGGCTGAGC CTGGAGCTGT GGAAGCTGTG CACCTCCCTC 
     4441 ACTTTGTGGC TCTCCAAGGG GGCCATGTGG ACACATCCCT GTTCCAAATG GCCCACTTTA 
     4501 AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGGA GCTGCATCAC ATAGTTCTGG 
     4561 AAAACCCCAG CTTCTCCCCC TTGGGAGTCC TCCTGAAAAT GATCCATAAT GCCCTGCGCT 
     4621 TCATTCCCGT CACCTCTGTG GTGTTGCTTT ACCACCGCGT CCATCCTGAG GAAGTCACCT 
     4681 TCCACCTCTA CCTGATCCCA AGTGACTGCT CCATTCGGAA GGAACTGGAG CTCTGCTATC 
     4741 GAAGCCCTGG AGAAGACCAG CTGTTCTCGG AGTTCTACGT TGGCCACTTG GGATCAGGGA 
     4801 TCAGGCTGCA AGTGAAAGAC AAGAAAGATG AGACTCTGGT GTGGGAGGCC TTGGTGAAAC 
     4861 CAGGAGATCT CATGCCTGCA ACTACTCTGA TCCCTCCAGC CCGCATAGCC GTACCTTCAC 
     4921 CTCTGGATGC CCCGCAGTTG CTGCACTTTG TGGACCAGTA TCGAGAGCAG CTGATAGCCC 
     4981 GAGTGACATC GGTGGAGGTT GTCTTGGACA AACTGCATGG ACAGGTGCTG AGCCAGGAGC 
     5041 AGTACGAGAG GGTGCTGGCT GAGAACACGA GGCCCAGCCA GATGCGGAAG CTGTTCAGCT 
     5101 TGAGCCAGTC CTGGGACCGG AAGTGCAAAG ATGGACTCTA CCAAGCCCTG AAGGAGACCC 
     5161 ATCCTCACCT CATTATGGAA CTCTGGGAGA AGGGCAGCAA AAAGGGACTC CTGCCACTCA 
     5221 GCAGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5281 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5341 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5401 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5461 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5521 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5581 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5641 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5701 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5761 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5821 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5881 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5941 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6001 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6061 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6121 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6181 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6241 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6301 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6361 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6421 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6481 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6541 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6601 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6661 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6721 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6781 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6841 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6901 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6961 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7021 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7081 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7141 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7201 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7261 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7321 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7381 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7441 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7501 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7561 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7621 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7681 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7741 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7801 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7861 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7921 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7981 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8041 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8101 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8161 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8221 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8281 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8341 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8401 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8461 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8521 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8581 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8641 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8701 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8761 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8821 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8881 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8941 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9001 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9061 AAATTTAACG CGAATTTT
//