LOCUS       RC215188                8601 bp    DNA     circular  SYN 
DEFINITION  INF2 (Myc-DDK-tagged)-Human inverted formin, FH2 and WH2 domain containing (INF2), transcript variant 2
ACCESSION   NM_001031714
VERSION     NM_001031714.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4758..4849 
                     /label="Myc-DDK"  
     misc_feature    4879..5464 
                     /label="PolyA signal"  
     misc_feature    4936..4955 
                     /label="Restriction Enzyme"  
     misc_feature    5740..6412 
                     /label="ColE1"  
     misc_feature    6842..7636 
                     /label="Kan/Neo"  
     misc_feature    7671..7973 
                     /label="SV40 ori"  
     misc_feature    8059..8536 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4740..4746 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4740 
                     /label="INF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGTGAAG GAGGGCGCAC AGCGCAAGTG GGCAGCGCTG AAGGAGAAGC 
     1081 TGGGGCCACA GGATTCGGAC CCCACGGAGG CCAACCTGGA GAGCGCGGAC CCCGAGCTGT 
     1141 GCATCCGGCT GCTCCAGATG CCCTCTGTGG TCAACTACTC CGGCCTGCGC AAGCGCCTGG 
     1201 AGGGCAGCGA CGGCGGCTGG ATGGTGCAGT TCCTGGAGCA GAGCGGCCTG GACCTGCTGC 
     1261 TGGAGGCGCT GGCGCGGCTG TCGGGCCGCG GCGTTGCACG TATCTCCGAC GCCCTGCTGC 
     1321 AGCTCACCTG CGTCAGCTGC GTGCGCGCCG TCATGAACTC GCGGCAGGGC ATCGAGTACA 
     1381 TCCTCAGCAA CCAGGGCTAC GTGCGCCAGC TCTCCCAGGC CCTGGACACA TCCAACGTGA 
     1441 TGGTGAAGAA GCAGGTGTTT GAGCTACTGG CTGCCCTGTG CATCTACTCT CCCGAGGGCC 
     1501 ACGTGCTGAC CCTGGACGCC CTGGACCACT ACAAGACGGT GTGCAGCCAG CAGTACCGCT 
     1561 TCAGCATTGT CATGAACGAG CTCTCCGGCA GCGACAACGT GCCCTACGTG GTCACCCTGC 
     1621 TTAGCGTGAT CAACGCCGTC ATCTTGGGCC CCGAGGACCT GCGCGCGCGC ACCCAGCTGC 
     1681 GGAACGAGTT TATCGGGCTG CAGCTGCTGG ACGTCCTGGC TCGCCTGCGA GACCTGGAGG 
     1741 ATGCCGACCT GCTGATCCAG CTGGAGGCTT TCGAGGAGGC TAAGGCCGAG GACGAGGAGG 
     1801 AGCTGCTGCG AGTCTCTGGC GGGGTCGACA TGAGCAGCCA CCAGGAGGTC TTTGCCTCCC 
     1861 TGTTCCACAA GGTGAGCTGC TCCCCGGTGT CTGCCCAGCT CCTGTCGGTG CTGCAGGGCC 
     1921 TCCTGCACCT GGAGCCCACC CTCCGCTCCA GCCAGCTGCT CTGGGAGGCC CTGGAGAGCC 
     1981 TCGTGAACCG GGCCGTGCTC CTGGCCAGCG ATGCCCAGGA ATGCACCCTG GAGGAAGTGG 
     2041 TTGAGCGGCT CCTGTCTGTC AAGGGGCGAC CCAGACCGAG CCCCCTGGTC AAGGCCCATA 
     2101 AAAGCGTCCA GGCCAACCTA GACCAGAGCC AGAGGGGCAG CTCCCCGCAA AACACTACAA 
     2161 CCCCCAAGCC CAGCGTGGAG GGCCAGCAGC CAGCAGCAGC TGCTGCCTGC GAGCCCGTGG 
     2221 ACCACGCCCA GAGTGAGAGC ATCCTGAAAG TTTCGCAGCC CAGAGCCCTG GAGCAGCAGG 
     2281 CGTCCACCCC ACCCCCACCC CCACCCCCAC CCCTGCTCCC TGGTTCCAGT GCCGAGCCCC 
     2341 CTCCCCCTCC CCCACCACCC CCCCTGCCCA GTGTGGGGGC TAAGGCCCTC CCAACAGCAC 
     2401 CCCCGCCCCC ACCCCTGCCA GGCCTGGGGG CCATGGCCCC CCCAGCACCT CCTCTACCAC 
     2461 CACCCCTGCC AGGCTCCTGT GAGTTCCTGC CCCCACCACC TCCACCACTC CCGGGCTTGG 
     2521 GATGCCCGCC CCCACCCCCA CCCCTGCTGC CTGGTATGGG CTGGGGCCCT CCTCCACCCC 
     2581 CACCTCCACT ACTGCCCTGC ACCTGCAGCC CCCCCGTGGC GGGAGGCATG GAGGAGGTCA 
     2641 TCGTGGCCCA GGTGGACCAT GGCTTGGGCT CAGCATGGGT CCCCAGCCAT CGGCGGGTGA 
     2701 ACCCACCCAC ACTGCGCATG AAGAAGCTGA ACTGGCAGAA GCTGCCATCC AACGTGGCAC 
     2761 GTGAGCACAA CTCTATGTGG GCGTCCCTGA GCAGCCCCGA CGCCGAGGCT GTGGAGCCCG 
     2821 ACTTCTCCAG CATCGAGCGA CTATTCTCCT TCCCTGCAGC CAAGCCCAAG GAGCCCACCA 
     2881 TGGTGGCCCC CCGGGCCAGG AAGGAGCCCA AGGAGATCAC TTTCCTCGAT GCCAAGAAGA 
     2941 GCCTGAACCT CAACATCTTC CTGAAGCAAT TTAAGTGCTC CAACGAGGAG GTCGCTGCTA 
     3001 TGATCCGGGC TGGAGATACC ACCAAGTTTG ATGTGGAGGT TCTCAAACAA CTCCTTAAGC 
     3061 TCCTTCCCGA GAAGCACGAG ATTGAAAACC TGCGGGCATT CACAGAGGAG CGAGCCAAGC 
     3121 TGGCCAGCGC CGACCACTTC TACCTCCTCC TGCTGGCCAT TCCCTGCTAC CAGCTGCGAA 
     3181 TCGAGTGCAT GCTGCTGTGT GAGGGCGCGG CCGCCGTGCT GGACATGGTG CGGCCCAAGG 
     3241 CCCAGCTGGT GCTGGCTGCC TGCGAAAGCC TGCTCACCAG CCGCCAGCTG CCCATCTTCT 
     3301 GCCAGCTGAT CCTGAGAATT GGGAACTTCC TCAACTACGG CAGCCACACC GGTGACGCCG 
     3361 ACGGCTTCAA GATCAGCACA TTGCTGAAGC TCACGGAGAC CAAGTCCCAG CAGAACCGCG 
     3421 TGACGCTGCT GCACCACGTG CTGGAGGAAG CGGAAAAGAG CCACCCCGAC CTCCTGCAGC 
     3481 TGCCCCGGGA CCTGGAACAG CCCTCGCAAG CAGCAGGGAT CAACCTGGAG ATCATCCGCT 
     3541 CAGAGGCCAG CTCCAACCTG AAGAAGCTTC TGGAGACCGA GCGGAAGGTG TCTGCCTCCG 
     3601 TGGCCGAGGT CCAGGAGCAG TACACCGAGC GCCTCCAGGC CAGCATCTCG GCCTTCCGGG 
     3661 CACTGGATGA GCTGTTTGAG GCCATCGAGC AGAAGCAACG GGAGCTGGCC GACTACCTGT 
     3721 GTGAGGACGC CCAGCAGCTG TCCCTGGAGG ACACGTTCAG CACCATGAAG GCTTTCCGGG 
     3781 ACCTTTTCCT CCGCGCCCTG AAGGAGAACA AGGACCGGAA GGAGCAGGCG GCGAAGGCAG 
     3841 AGAGGAGGAA GCAGCAGCTG GCGGAGGAGG AGGCGCGGCG GCCTCGGGGA GAGGACGGGA 
     3901 AGCCTGTCAG GAAGGGGCCC GGGAAGCAGG AGGAGGTGTG TGTCATCGAT GCCCTGCTGG 
     3961 CTGACATCAG GAAGGGCTTC CAGCTGCGGA AGACAGCCCG GGGCCGCGGG GACACCGACG 
     4021 GGGGCAGCAA GGCAGCCTCC ATGGATCCCC CAAGAGCCAC AGAGCCTGTG GCCACCAGTA 
     4081 ACCCTGCAGG AGATCCCGTG GGCAGCACGC GCTGTCCCGC CTCTGAGCCC GGCCTTGATG 
     4141 CTACAACAGC CAGCGAGTCC CGGGGCTGGG ACCTTGTAGA CGCCGTGACC CCCGGCCCTC 
     4201 AGCCCACCCT GGAGCAGTTG GAGGAGGGTG GTCCACGGCC CCTGGAGAGG CGTTCTTCCT 
     4261 GGTATGTGGA TGCCAGCGAT GTCCTAACCA CTGAGGATCC CCAGTGCCCC CAGCCCTTGG 
     4321 AGGGGGCCTG GCCGGTGACT CTGGGAGATG CTCAGGCCCT GAAGCCCCTC AAGTTCTCCA 
     4381 GCAACCAGCC CCCTGCAGCC GGAAGTTCAA GGCAAGATGC CAAGGATCCC ACGTCCTTGC 
     4441 TGGGCGTCCT CCAGGCCGAG GCCGACAGCA CAAGTGAGGG GCTGGAGGAC GCTGTCCACA 
     4501 GCCGTGGTGC CAGACCCCCT GCAGCAGGCC CAGGTGGGGA TGAGGACGAG GACGAGGAGG 
     4561 ACACGGCCCC AGAGTCCGCA CTGGACACAT CCCTGGACAA GTCCTTCTCC GAGGATGCGG 
     4621 TGACCGACTC CTCGGGGTCG GGCACACTCC CCAGGGCCCG GGGCCGGGCC TCAAAGGGGA 
     4681 CCGGGAAGCG AAGGAAGAAG CGTCCCTCCA GGAGCCAGGA AGGCCTCAGG CCCAGGCCCA 
     4741 AGGCCAAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4801 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4861 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4921 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4981 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5041 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5101 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5161 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5221 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5281 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5341 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5401 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5461 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5521 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5581 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5641 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5701 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5761 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5821 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5881 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5941 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6001 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6061 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6121 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6181 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6241 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6301 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6361 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6421 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6481 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6541 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6601 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6661 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6721 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6781 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6841 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6901 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6961 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7021 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7081 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7141 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7201 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7261 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7321 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7381 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7441 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7501 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7561 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7621 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7681 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7741 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7801 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8041 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8101 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8161 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8221 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8281 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8341 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8401 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8461 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8521 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8581 CAAAAATTTA ACGCGAATTT T
//