LOCUS       RC215160                9378 bp    DNA     circular  SYN 
DEFINITION  SYNJ2 (Myc-DDK-tagged)-Human synaptojanin 2 (SYNJ2), transcript variant 1
ACCESSION   NM_003898
VERSION     NM_003898.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5533..5624 
                     /label="Myc-DDK"  
     misc_feature    5654..6239 
                     /label="PolyA signal"  
     misc_feature    5711..5730 
                     /label="Restriction Enzyme"  
     misc_feature    6515..7187 
                     /label="ColE1"  
     misc_feature    7617..8411 
                     /label="Kan/Neo"  
     misc_feature    8446..8748 
                     /label="SV40 ori"  
     misc_feature    8834..9311 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5508..5515 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5508 
                     /label="SYNJ2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTGAGC AAAGGGCTGC GGCTGCTGGG GCGCCTGGGG GCCGAGGGGG 
     1081 ACTGTAGCGT GCTGCTGGAG GCGCGCGGCC GCGACGACTG CCTGCTGTTC GAGGCCGGCA 
     1141 CGGTGGCCAC GCTGGCTCCA GAAGAAAAGG AAGTCATTAA AGGACAGTAT GGCAAGCTCA 
     1201 CGGACGCGTA CGGCTGCCTG GGGGAGCTGA GGCTGAAATC TGGTGGCACG TCTCTGAGCT 
     1261 TCCTGGTGTT GGTGACAGGC TGCACATCTG TGGGCAGAAT TCCAGATGCT GAAATCTACA 
     1321 AAATCACTGC CACTGACTTT TACCCTCTTC AGGAAGAGGC CAAGGAGGAG GAACGCCTCA 
     1381 TAGCTTTGAA GAAAATCCTC AGCTCGGGGG TGTTCTATTT CTCATGGCCA AACGATGGGT 
     1441 CTCGCTTTGA CCTGACTGTC CGCACGCAGA AGCAGGGGGA TGACAGCTCT GAATGGGGGA 
     1501 ACTCCTTCTT CTGGAACCAG CTGTTGCACG TGCCCTTGAG GCAGCACCAG GTGAGCTGCT 
     1561 GTGACTGGCT GCTGAAGATC ATCTGCGGGG TGGTCACCAT CCGCACCGTG TATGCCTCCC 
     1621 ACAAGCAGGC CAAGGCCTGC CTCGTCTCTC GCGTTAGCTG TGAGCGCACA GGCACTCGCT 
     1681 TCCACACCCG TGGCGTGAAC GACGACGGCC ATGTGTCCAA CTTCGTGGAG ACAGAGCAGA 
     1741 TGATTTACAT GGACGATGGA GTGTCATCTT TTGTCCAGAT CAGAGGCTCC GTTCCGCTGT 
     1801 TCTGGGAACA GCCAGGGCTT CAGGTTGGCT CCCATCATCT GAGACTCCAC AGAGGCCTGG 
     1861 AAGCCAATGC CCCTGCTTTC GACAGGCACA TGGTGCTTCT GAAGGAGCAG TACGGGCAGC 
     1921 AGGTGGTCGT GAACCTTCTG GGAAGCAGAG GCGGAGAGGA GGTGCTCAAC AGAGCCTTCA 
     1981 AGAAGCTGCT CTGGGCTTCT TGCCACGCGG GCGACACGCC TATGATCAAT TTTGACTTCC 
     2041 ATCAGTTTGC CAAAGGTGGG AAGCTAGAGA AATTGGAGAC CCTCTTGAGG CCACAGTTAA 
     2101 AGCTGCACTG GGAAGACTTC GATGTGTTCA CAAAGGGGGA GAACGTCAGT CCACGTTTTC 
     2161 AGAAAGGCAC TTTGCGGATG AACTGTCTTG ACTGCCTGGA CCGAACCAAC ACTGTGCAGA 
     2221 GCTTCATCGC GCTCGAGGTC CTGCATCTGC AGCTCAAGAC CCTGGGGCTG AGTTCAAAAC 
     2281 CCATCGTTGA CCGCTTTGTG GAGTCCTTCA AAGCCATGTG GTCTCTGAAT GGCCACAGCC 
     2341 TGAGCAAGGT GTTCACAGGC AGCAGAGCCC TGGAAGGGAA GGCCAAGGTG GGGAAGCTGA 
     2401 AGGATGGAGC CCGGTCCATG TCTCGAACCA TCCAGTCCAA CTTCTTCGAC GGGGTGAAGC 
     2461 AGGAGGCCAT CAAGCTGCTG CTGGTTGGGG ACGTCTACGG CGAGGAGGTG GCAGACAAAG 
     2521 GGGGCATGCT GCTGGACAGC ACGGCGCTCC TGGTGACTCC CAGGATCCTG AAAGCTATGA 
     2581 CTGAGCGTCA GTCCGAATTC ACAAATTTCA AGCGGATCCG GATTGCTATG GGGACCTGGA 
     2641 ACGTGAACGG AGGAAAGCAG TTCCGGAGCA ACGTGCTCAG GACGGCGGAG CTGACAGACT 
     2701 GGCTGCTCGA CTCGCCCCAG CTCTCGGGAG CTACCGACTC CCAGGATGAC AGCAGCCCAG 
     2761 CTGACATATT TGCTGTGGGG TTTGAAGAGA TGGTGGAATT GAGCGCAGGG AATATTGTCA 
     2821 ATGCCAGTAC TACCAACAAG AAGATGTGGG GTGAACAGCT TCAGAAAGCC ATCTCACGCT 
     2881 CTCATAGATA CATTCTGTTG ACTTCGGCAC AGCTGGTGGG CGTCTGTCTT TATATCTTTG 
     2941 TACGTCCATA CCATGTCCCG TTCATCAGGG ACGTAGCCAT CGACACAGTG AAGACGGGCA 
     3001 TGGGGGGCAA GGCGGGGAAC AAGGGCGCCG TCGGCATCCG CTTCCAGTTC CACAGCACCA 
     3061 GCTTCTGCTT CATATGTAGT CACCTGACGG CCGGGCAGTC CCAGGTGAAG GAGCGGAATG 
     3121 AAGACTACAA GGAGATCACC CAGAAACTCT GCTTCCCAAT GGGGAGAAAT GTTTTTTCTC 
     3181 ATGATTATGT ATTTTGGTGT GGCGATTTCA ACTACCGCAT TGATCTTACT TATGAAGAAG 
     3241 TCTTCTATTT TGTTAAACGC CAAGACTGGA AGAAACTTCT GGAATTTGAT CAACTACAGC 
     3301 TACAGAAATC AAGTGGAAAA ATTTTTAAGG ACTTTCACGA AGGAGCCATT AACTTTGGAC 
     3361 CCACCTACAA GTATGACGTC GGCTCAGCCG CCTACGATAC AAGCGACAAA TGCCGCACCC 
     3421 CCGCCTGGAC AGACAGGGTG CTGTGGTGGA GGAAGAAACA TCCCTTTGAT AAAACAGCTG 
     3481 GAGAACTCAA CCTTCTAGAC AGTGATCTAG ATGTTGACAC CAAAGTCAGA CACACCTGGT 
     3541 CTCCTGGTGC CCTGCAGTAT TATGGTCGTG CGGAGCTACA AGCTTCTGAT CACAGACCTG 
     3601 TGCTGGCGAT CGTGGAGGTG GAAGTTCAGG AAGTCGATGT GGGTGCTCGG GAGAGGGTTT 
     3661 TCCAGGAAGT GTCCTCCTTC CAGGGCCCCC TGGATGCCAC TGTTGTAGTA AACCTTCAAT 
     3721 CACCGACCTT AGAAGAGAAA AACGAGTTTC CAGAGGACCT GCGTACTGAG CTCATGCAGA 
     3781 CCTTGGGGAG TTATGGGACA ATTGTTCTTG TCAGGATCAA CCAAGGGCAG ATGCTGGTAA 
     3841 CTTTTGCAGA CAGTCACTCG GCTCTCAGTG TCCTGGACGT GGACGGTATG AAGGTGAAAG 
     3901 GCAGAGCAGT GAAGATTAGA CCGAAGACCA AGGACTGGCT GAAAGGTTTG CGAGAGGAGA 
     3961 TCATTCGGAA ACGAGACAGC ATGGCCCCCG TGTCTCCCAC TGCCAACTCC TGTTTGCTGG 
     4021 AGGAAAACTT TGACTTCACA AGTTTGGACT ATGAGTCAGA AGGGGATATT CTTGAAGACG 
     4081 ATGAAGACTA CTTGGTGGAT GAATTCAATC AGCCTGGGGT CTCGGACAGT GAACTCGGGG 
     4141 GAGACGACCT CTCTGATGTC CCCGGCCCCA CAGCACTGGC TCCTCCCAGC AAGTCACCTG 
     4201 CTCTCACCAA AAAGAAGCAG CATCCAACGT ACAAAGATGA CGCGGACCTG GTGGAGCTCA 
     4261 AGCGGGAGCT GGAAGCCGTC GGGGAGTTCC GCCACCGTTC TCCGAGCAGG TCTCTGTCGG 
     4321 TCCCCAACCG ACCTCGGCCA CCTCAACCCC CGCAGAGACC CCCCCCTCCA ACCGGTTTAA 
     4381 TGGTGAAAAA GTCGGCTTCA GATGCGTCCA TCTCCTCCGG CACCCATGGG CAGTATTCAA 
     4441 TTTTGCAGAC GGCAAGACTT CTACCAGGAG CACCTCAGCA ACCTCCCAAG GCTCGGACTG 
     4501 GAATAAGTAA ACCTTATAAT GTCAAGCAGA TCAAAACCAC CAATGCCCAG GAGGCAGAAG 
     4561 CAGCAATCCG GTGTCTCCTG GAAGCCAGAG GAGGTGCCTC CGAAGAAGCC CTAAGTGCCG 
     4621 TGGCCCCAAG GGACCTTGAA GCATCCTCTG AACCAGAGCC CACACCGGGG GCAGCCAAAC 
     4681 CAGAGACCCC ACAGGCGCCC CCACTCCTTC CCCGTCGGCC CCCACCCAGA GTTCCTGCCA 
     4741 TCAAGAAGCC AACCTTGAGA AGGACAGGAA AGCCCCTGTC ACCGGAAGAA CAGTTTGAGC 
     4801 AACAGACTGT CCATTTTACA ATCGGGCCCC CGGAGACAAG CGTTGAGGCC CCTCCTGTCG 
     4861 TGACAGCCCC TCGAGTCCCT CCTGTTCCCA AACCAAGAAC ATTTCAGCCT GGGAAAGCTG 
     4921 CAGAGAGGCC AAGCCACAGG AAGCCAGCAT CAGACGAAGC CCCTCCTGGG GCAGGAGCCT 
     4981 CTGTGCCACC ACCTCTGGAG GCGCCGCCTC TTGTGCCCAA GGTACCCCCG AGGAGGAAGA 
     5041 AGTCAGCCCC CGCAGCCTTC CACCTGCAGG TCCTGCAGAG CAACAGCCAG CTTCTCCAGG 
     5101 GCCTCACTTA CAATAGCAGT GACAGCCCCT CTGGGCACCC ACCTGCCGCG GGCACCGTCT 
     5161 TCCCACAAGG GGACTTTCTC AGCACTTCAT CTGCTACAAG CCCCGACAGC GATGGCACCA 
     5221 AAGCGATGAA GCCAGAGGCA GCCCCACTTC TTGGTGATTA TCAGGACCCC TTCTGGAACC 
     5281 TTCTTCACCA CCCTAAACTG TTGAATAACA CTTGGCTTTC TAAGAGCTCA GACCCTTTGG 
     5341 ACTCAGGAAC CAGGAGCCCC AAAAGAGATC CCATAGACCC AGTGTCAGCT GGCGCTTCAG 
     5401 CTGCCAAGGC AGAGCTGCCA CCAGATCATG GACACAAAAC CTTAGGTCAC TGGGTGACAA 
     5461 TCAGTGACCA AGAAAAGAGG ACAGCACTGC AGGTGTTTGA CCCACTGGCA AAAACAAGCG 
     5521 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5581 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5641 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5701 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5761 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5821 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5881 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5941 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     6001 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     6061 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6121 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6181 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6241 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6301 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6361 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6421 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6481 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6541 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6601 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6661 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6721 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6781 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6841 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6901 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6961 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7021 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7081 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7141 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7201 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7261 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7321 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7381 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7441 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7501 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7561 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7621 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7681 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7741 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7801 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7861 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7921 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7981 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     8041 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     8101 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8161 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8221 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8281 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8341 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8401 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8461 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8521 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8581 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8641 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8701 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8761 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8821 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8881 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8941 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     9001 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     9061 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9121 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9181 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9241 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9301 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9361 AAATTTAACG CGAATTTT
//