LOCUS       RC215127                8685 bp    DNA     circular  SYN 
DEFINITION  STAG2 (Myc-DDK-tagged)-Human stromal antigen 2 (STAG2), transcript variant 2
ACCESSION   NM_001042750
VERSION     NM_001042750.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4842..4933 
                     /label="Myc-DDK"  
     misc_feature    4963..5548 
                     /label="PolyA signal"  
     misc_feature    5020..5039 
                     /label="Restriction Enzyme"  
     misc_feature    5824..6496 
                     /label="ColE1"  
     misc_feature    6926..7720 
                     /label="Kan/Neo"  
     misc_feature    7755..8057 
                     /label="SV40 ori"  
     misc_feature    8143..8620 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4824..4830 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4824 
                     /label="STAG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGCAGCT CCAGAAATAC CAACTGATTT TAATCTACTA CAGGAGTCAG 
     1081 AAACACATTT TTCTTCTGAC ACAGATTTTG AAGATATCGA AGGAAAAAAC CAAAAGCAAG 
     1141 GCAAAGGCAA AACTTGTAAA AAAGGCAAAA AGGGCCCAGC AGAAAAGGGC AAAGGTGGAA 
     1201 ATGGAGGAGG AAAACCTCCT TCTGGTCCAA ACCGAATGAA TGGTCATCAC CAACAGAATG 
     1261 GAGTGGAAAA CATGATGTTG TTTGAAGTTG TTAAAATGGG CAAGAGTGCT ATGCAGTCGG 
     1321 TGGTAGATGA TTGGATAGAA TCATACAAGC ATGACCGAGA TATAGCACTT CTTGACCTTA 
     1381 TCAACTTTTT TATTCAGTGT TCAGGCTGTA AAGGAGTTGT CACAGCAGAA ATGTTTAGAC 
     1441 ATATGCAGAA CTCTGAGATA ATTCGAAAAA TGACTGAAGA ATTCGATGAG GATAGTGGAG 
     1501 ATTATCCACT TACCATGGCT GGTCCTCAGT GGAAGAAGTT CAAATCCAGT TTTTGTGAAT 
     1561 TCATTGGCGT GTTAGTACGG CAATGTCAAT ATAGTATCAT ATATGATGAG TATATGATGG 
     1621 ATACAGTCAT TTCACTTCTT ACAGGATTGT CTGACTCACA AGTCAGAGCA TTTCGACATA 
     1681 CAAGCACCCT GGCAGCTATG AAGTTGATGA CAGCTTTGGT GAATGTGGCA CTAAATCTTA 
     1741 GCATTAATAT GGATAATACA CAAAGACAAT ATGAAGCAGA ACGGAATAAA ATGATTGGAA 
     1801 AACGAGCCAA TGAGAGGCTA GAACTCCTGC TACAAAAGCG GAAAGAGCTT CAGGAAAATC 
     1861 AAGATGAAAT AGAAAATATG ATGAATGCAA TATTTAAAGG AGTGTTTGTA CATAGATACC 
     1921 GTGATGCGAT AGCTGAAATT CGAGCTATTT GCATTGAAGA GATTGGCATT TGGATGAAGA 
     1981 TGTATAGTGA TGCCTTTCTT AATGACAGTT ATTTAAAATA TGTTGGTTGG ACTATGCATG 
     2041 ATAAGCAAGG TGAAGTAAGA CTCAAATGTC TTACTGCTCT ACAAGGGCTT TATTATAACA 
     2101 AAGAGCTTAA TTCCAAACTG GAACTTTTTA CCAGTCGGTT CAAGGATAGA ATTGTGTCTA 
     2161 TGACCCTTGA CAAAGAATAT GATGTTGCAG TACAAGCAAT AAAATTACTC ACTCTTGTTT 
     2221 TACAGAGTAG TGAAGAAGTT CTCACTGCAG AAGATTGTGA AAATGTCTAT CATCTGGTTT 
     2281 ATTCAGCTCA CCGGCCAGTA GCAGTAGCAG CTGGAGAATT TCTCTACAAA AAGCTCTTCA 
     2341 GTCGTAGAGA TCCAGAGGAG GATGGAATGA TGAAAAGAAG AGGAAGACAA GGTCCAAATG 
     2401 CCAACCTTGT TAAGACATTG GTTTTTTTCT TTCTAGAAAG TGAGTTACAT GAGCATGCAG 
     2461 CATACCTTGT GGATAGCATG TGGGACTGTG CTACTGAGCT GCTGAAAGAC TGGGAATGTA 
     2521 TGAATAGCTT GTTACTGGAA GAGCCACTTA GTGGAGAGGA AGCACTAACA GATAGGCAAG 
     2581 AGAGTGCTCT GATTGAAATA ATGCTTTGTA CCATTAGACA AGCGGCTGAA TGTCATCCTC 
     2641 CCGTGGGAAG AGGGACAGGA AAAAGGGTGC TTACAGCAAA GGAGAAGAAG ACACAGTTGG 
     2701 ATGATAGGAC AAAAATCACT GAGCTTTTTG CCGTGGCCCT TCCTCAGTTA TTAGCAAAAT 
     2761 ACTCTGTAGA TGCAGAAAAG GTGACTAACT TGTTGCAGTT GCCTCAGTAC TTTGATTTGG 
     2821 AAATATATAC CACTGGACGA TTAGAAAAGC ATTTGGATGC CTTATTGCGA CAGATCCGGA 
     2881 ATATTGTAGA GAAGCACACA GATACAGATG TTTTGGAAGC ATGTTCTAAA ACTTACCATG 
     2941 CACTCTGTAA TGAAGAGTTC ACAATCTTCA ACAGAGTAGA TATTTCAAGA AGTCAACTGA 
     3001 TAGATGAATT GGCAGATAAA TTTAACCGGC TTCTTGAAGA TTTTCTGCAA GAGGGTGAAG 
     3061 AACCTGATGA AGATGATGCA TATCAGGTAT TGTCAACATT GAAGAGGATC ACTGCTTTTC 
     3121 ATAATGCCCA TGACCTTTCA AAGTGGGATT TATTTGCTTG TAATTACAAA CTCTTGAAAA 
     3181 CTGGAATCGA AAATGGAGAC ATGCCTGAGC AGATTGTTAT TCACGCACTG CAGTGTACTC 
     3241 ACTATGTAAT CCTTTGGCAA CTTGCTAAGA TAACTGAAAG CAGCTCTACA AAGGAGGACT 
     3301 TGCTGCGTTT AAAGAAACAA ATGAGAGTAT TTTGTCAGAT ATGTCAACAT TACCTGACCA 
     3361 ACGTGAATAC TACTGTTAAG GAACAGGCCT TCACTATTCT GTGTGATATT TTGATGATCT 
     3421 TCAGCCATCA GATTATGTCA GGAGGGCGTG ACATGTTAGA GCCATTAGTG TATACCCCTG 
     3481 ATTCTTCATT GCAGTCTGAG TTGCTCAGCT TTATTTTGGA TCATGTCTTC ATTGAACAGG 
     3541 ATGATGATAA TAATAGTGCA GATGGTCAGC AAGAGGATGA AGCCAGTAAA ATTGAAGCTC 
     3601 TGCACAAGAG AAGAAATTTA CTTGCAGCAT TTTGTAAGCT AATTGTATAT ACTGTGGTGG 
     3661 AGATGAATAC AGCTGCAGAT ATCTTCAAAC AGTATATGAA GTATTATAAT GACTATGGAG 
     3721 ATATCATCAA AGAAACAATG AGTAAAACAA GGCAGATAGA CAAAATTCAG TGTGCTAAGA 
     3781 CCCTTATTCT CAGTCTGCAA CAGCTTTTTA ATGAAATGAT ACAAGAAAAT GGCTATAATT 
     3841 TTGATAGATC ATCCTCTACA TTTAGTGGCA TAAAAGAACT TGCTCGACGT TTTGCTTTAA 
     3901 CTTTTGGACT TGATCAGTTG AAAACAAGAG AAGCCATTGC CATGCTACAC AAAGATGGCA 
     3961 TAGAATTTGC TTTTAAAGAG CCTAATCCGC AAGGGGAGAG CCATCCACCT TTAAATTTGG 
     4021 CATTTCTTGA TATTCTGAGT GAATTTTCTT CTAAACTACT TCGACAAGAC AAAAGAACAG 
     4081 TGTATGTTTA CTTGGAAAAG TTCATGACCT TTCAGATGTC ACTCCGAAGA GAGGATGTGT 
     4141 GGCTTCCACT GATGTCTTAC CGAAATTCTT TGCTAGCTGG TGGTGATGAT GACACCATGT 
     4201 CAGTCATTAG TGGAATCAGC AGCCGGGGGT CAACAGTACG GAGTAAAAAA TCAAAACCAT 
     4261 CTACAGGAAA ACGGAAAGTG GTTGAGGGCA TGCAGCTTTC ACTCACTGAA GAAAGTAGTA 
     4321 GTAGTGACAG TATGTGGTTA AGCAGAGAAC AAACACTGCA CACCCCTGTT ATGATGCAGA 
     4381 CACCACAACT CACCTCCACT ATTATGAGAG AGCCCAAAAG ATTACGGCCT GAGGATAGCT 
     4441 TCATGAGTGT TTATCCAATG CAGACTGAAC ATCATCAAAC ACCTCTTGAT TATAACACGC 
     4501 AGGTAACATG GATGTTAGCT CAAAGACAAC AAGAGGAAGC AAGGCAACAG CAGGAGAGAG 
     4561 CAGCAATGAG CTATGTTAAA CTGCGAACTA ATCTTCAGCA TGCCATTCGG CGTGGCACAA 
     4621 GCCTAATGGA AGATGATGAA GAGCCAATTG TGGAAGATGT TATGATGTCC TCAGAAGGGA 
     4681 GGATTGAGGA TCTTAATGAG GGAATGGATT TTGACACCAT GGATATAGAT TTGCCACCAT 
     4741 CAAAGAACAG ACGAGAGAGA ACAGAACTGA AGCCTGATTT CTTTGATCCA GCTTCAATTA 
     4801 TGGATGAATC AGTTCTTGGA GTGTCAATGT TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4861 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4921 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4981 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5041 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5101 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5161 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5221 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5281 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5341 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5401 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5461 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5521 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5581 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5641 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5701 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5761 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5821 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5881 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5941 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6001 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6061 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6121 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6181 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6241 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6301 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6361 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6421 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6481 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6541 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6601 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6661 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6721 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6781 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6841 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6901 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6961 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7021 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7081 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7141 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7201 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7261 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7321 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7381 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7441 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7501 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7561 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7621 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7681 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7741 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7801 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7861 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7921 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8101 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8161 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8221 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8281 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8341 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8401 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8461 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8521 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8581 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8641 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//