LOCUS       RC215123L1                9129 bp    DNA     circular  SYN 
DEFINITION  IL6ST (Myc-DDK-tagged)-Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1
ACCESSION   NM_002184
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7420..7600 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8328..8987 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7759..8327 
                     /label="ColE1"  
     misc_feature    6295..6315 
                     /label="LR50 primer"  
     misc_feature    6092..6163 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6304..6895 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6074..6080 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6074 
                     /label="IL6ST"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTGACGTTG CAGACTTGGC TAGTGCAAGC 
     3361 CTTGTTTATT TTCCTCACCA CTGAATCTAC AGGTGAACTT CTAGATCCAT GTGGTTATAT 
     3421 CAGTCCTGAA TCTCCAGTTG TACAACTTCA TTCTAATTTC ACTGCAGTTT GTGTGCTAAA 
     3481 GGAAAAATGT ATGGATTATT TTCATGTAAA TGCTAATTAC ATTGTCTGGA AAACAAACCA 
     3541 TTTTACTATT CCTAAGGAGC AATATACTAT CATAAACAGA ACAGCATCCA GTGTCACCTT 
     3601 TACAGATATA GCTTCATTAA ATATTCAGCT CACTTGCAAC ATTCTTACAT TCGGACAGCT 
     3661 TGAACAGAAT GTTTATGGAA TCACAATAAT TTCAGGCTTG CCTCCAGAAA AACCTAAAAA 
     3721 TTTGAGTTGC ATTGTGAACG AGGGGAAGAA AATGAGGTGT GAGTGGGATG GTGGAAGGGA 
     3781 AACACACTTG GAGACAAACT TCACTTTAAA ATCTGAATGG GCAACACACA AGTTTGCTGA 
     3841 TTGCAAAGCA AAACGTGACA CCCCCACCTC ATGCACTGTT GATTATTCTA CTGTGTATTT 
     3901 TGTCAACATT GAAGTCTGGG TAGAAGCAGA GAATGCCCTT GGGAAGGTTA CATCAGATCA 
     3961 TATCAATTTT GATCCTGTAT ATAAAGTGAA GCCCAATCCG CCACATAATT TATCAGTGAT 
     4021 CAACTCAGAG GAACTGTCTA GTATCTTAAA ATTGACATGG ACCAACCCAA GTATTAAGAG 
     4081 TGTTATAATA CTAAAATATA ACATTCAATA TAGGACCAAA GATGCCTCAA CTTGGAGCCA 
     4141 GATTCCTCCT GAAGACACAG CATCCACCCG ATCTTCATTC ACTGTCCAAG ACCTTAAACC 
     4201 TTTTACAGAA TATGTGTTTA GGATTCGCTG TATGAAGGAA GATGGTAAGG GATACTGGAG 
     4261 TGACTGGAGT GAAGAAGCAA GTGGGATCAC CTATGAAGAT AGACCATCTA AAGCACCAAG 
     4321 TTTCTGGTAT AAAATAGATC CATCCCATAC TCAAGGCTAC AGAACTGTAC AACTCGTGTG 
     4381 GAAGACATTG CCTCCTTTTG AAGCCAATGG AAAAATCTTG GATTATGAAG TGACTCTCAC 
     4441 AAGATGGAAA TCACATTTAC AAAATTACAC AGTTAATGCC ACAAAACTGA CAGTAAATCT 
     4501 CACAAATGAT CGCTATCTAG CAACCCTAAC AGTAAGAAAT CTTGTTGGCA AATCAGATGC 
     4561 AGCTGTTTTA ACTATCCCTG CCTGTGACTT TCAAGCTACT CACCCTGTAA TGGATCTTAA 
     4621 AGCATTCCCC AAAGATAACA TGCTTTGGGT GGAATGGACT ACTCCAAGGG AATCTGTAAA 
     4681 GAAATATATA CTTGAGTGGT GTGTGTTATC AGATAAAGCA CCCTGTATCA CAGACTGGCA 
     4741 ACAAGAAGAT GGTACCGTGC ATCGCACCTA TTTAAGAGGG AACTTAGCAG AGAGCAAATG 
     4801 CTATTTGATA ACAGTTACTC CAGTATATGC TGATGGACCA GGAAGCCCTG AATCCATAAA 
     4861 GGCATACCTT AAACAAGCTC CACCTTCCAA AGGACCTACT GTTCGGACAA AAAAAGTAGG 
     4921 GAAAAACGAA GCTGTCTTAG AGTGGGACCA ACTTCCTGTT GATGTTCAGA ATGGATTTAT 
     4981 CAGAAATTAT ACTATATTTT ATAGAACCAT CATTGGAAAT GAAACTGCTG TGAATGTGGA 
     5041 TTCTTCCCAC ACAGAATATA CATTGTCCTC TTTGACTAGT GACACATTGT ACATGGTACG 
     5101 AATGGCAGCA TACACAGATG AAGGTGGGAA GGATGGTCCA GAATTCACTT TTACTACCCC 
     5161 AAAGTTTGCT CAAGGAGAAA TTGAAGCCAT AGTCGTGCCT GTTTGCTTAG CATTCCTATT 
     5221 GACAACTCTT CTGGGAGTGC TGTTCTGCTT TAATAAGCGA GACCTAATTA AAAAACACAT 
     5281 CTGGCCTAAT GTTCCAGATC CTTCAAAGAG TCATATTGCC CAGTGGTCAC CTCACACTCC 
     5341 TCCAAGGCAC AATTTTAATT CAAAAGATCA AATGTATTCA GATGGCAATT TCACTGATGT 
     5401 AAGTGTTGTG GAAATAGAAG CAAATGACAA AAAGCCTTTT CCAGAAGATC TGAAATCATT 
     5461 GGACCTGTTC AAAAAGGAAA AAATTAATAC TGAAGGACAC AGCAGTGGTA TTGGGGGGTC 
     5521 TTCATGCATG TCATCTTCTA GGCCAAGCAT TTCTAGCAGT GATGAAAATG AATCTTCACA 
     5581 AAACACTTCG AGCACTGTCC AGTATTCTAC CGTGGTACAC AGTGGCTACA GACACCAAGT 
     5641 TCCGTCAGTC CAAGTCTTCT CAAGATCCGA GTCTACCCAG CCCTTGTTAG ATTCAGAGGA 
     5701 GCGGCCAGAA GATCTACAAT TAGTAGATCA TGTAGATGGC GGTGATGGTA TTTTGCCCAG 
     5761 GCAACAGTAC TTCAAACAGA ACTGCAGTCA GCATGAATCC AGTCCAGATA TTTCACATTT 
     5821 TGAAAGGTCA AAGCAAGTTT CATCAGTCAA TGAGGAAGAT TTTGTTAGAC TTAAACAGCA 
     5881 GATTTCAGAT CATATTTCAC AATCCTGTGG ATCTGGGCAA ATGAAAATGT TTCAGGAAGT 
     5941 TTCTGCAGCA GATGCTTTTG GTCCAGGTAC TGAGGGACAA GTAGAAAGAT TTGAAACAGT 
     6001 TGGCATGGAG GCTGCGACTG ATGAAGGCAT GCCTAAAAGT TACTTACCAC AGACTGTACG 
     6061 GCAAGGCGGC TACATGCCTC AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     6121 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC 
     6181 GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT 
     6241 ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC 
     6301 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 
     6361 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 
     6421 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 
     6481 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 
     6541 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 
     6601 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 
     6661 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 
     6721 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 
     6781 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 
     6841 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 
     6901 CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA 
     6961 TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG 
     7021 TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 
     7081 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 
     7141 AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA 
     7201 CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC 
     7261 AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC 
     7321 TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG 
     7381 CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG 
     7441 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC 
     7501 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG 
     7561 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC 
     7621 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     7681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     7741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     7801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     7861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     7921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     7981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     8041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     8101 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     8161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     8221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     8281 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC 
     8341 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG 
     8401 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT 
     8461 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG 
     8521 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA 
     8581 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT 
     8641 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA 
     8701 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG 
     8761 TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA 
     8821 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC 
     8881 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT 
     8941 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC 
     9001 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9061 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTCCCGC GCACATTTCC CCGAAAAGTG 
     9121 CCACCTGAC
//