LOCUS       RC215099                8424 bp    DNA     circular  SYN 
DEFINITION  KCNMA1 (Myc-DDK-tagged)-Human potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 2
ACCESSION   NM_002247
VERSION     NM_002247.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4579..4670 
                     /label="Myc-DDK"  
     misc_feature    4700..5285 
                     /label="PolyA signal"  
     misc_feature    4757..4776 
                     /label="Restriction Enzyme"  
     misc_feature    5561..6233 
                     /label="ColE1"  
     misc_feature    6663..7457 
                     /label="Kan/Neo"  
     misc_feature    7492..7794 
                     /label="SV40 ori"  
     misc_feature    7880..8357 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4561 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="KCNMA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAATGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGAGGCAG CAGTCTTAGA ATGAGTAGCA ATATCCACGC GAACCATCTC AGCCTAGACG 
     1141 CGTCCTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT CCCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCCT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTGT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT TAACAATGGC TCAAGCCAGG 
     1441 CGGATGGCAC TCTCAAACCA GTGGATGAAA AAGAGGAGGC AGTGGCCGCC GAGGTCGGCT 
     1501 GGATGACCTC CGTGAAGGAC TGGGCGGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTCTTAGTC TTTGCTCTCA GCATCGGTGC ACTTGTAATA TACTTCATAG 
     1621 ATTCATCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTTCTCT ACTTCGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAATTGTG GTTCTGGCTG GAAGTGAACT CTGTAGTGGA TTTCTTCACG GTGCCCCCCG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGTTT GAGATTTTTA AGAGCTCTGA 
     1861 GACTGATACA GTTTTCAGAA ATTTTGCAGT TTCTGAATAT TCTTAAAACA AGTAATTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCCGGGTTCA 
     1981 TCCATTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCTCTCA 
     2041 CCTACTGGGA ATGTGTCTAT TTACTCATGG TCACAATGTC CACCGTTGGT TATGGGGATG 
     2101 TTTATGCAAA AACCACACTT GGGCGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGTGCGGTT AGTGGAAGAA AGCACATTGT GGTCTGCGGA CACATCACTC 
     2281 TGGAGAGTGT TTCCAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GACGTCAATG 
     2341 TGGAGATCGT TTTTCTTCAC AACATCTCCC CCAACCTGGA GCTTGAAGCT CTGTTCAAAC 
     2401 GACATTTTAC TCAGGTGGAA TTTTATCAGG GTTCCGTCCT CAATCCACAT GATCTTGCAA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAACAAGTAC TGCGCTGACC 
     2521 CGGATGCGGA GGATGCCTCG AATATCATGA GAGTAATCTC CATAAAGAAC TACCATCCGA 
     2581 AGATAAGAAT CATCACTCAA ATGCTGCAGT ATCACAACAA GGCCCATCTG CTAAACATCC 
     2641 CGAGCTGGAA TTGGAAAGAA GGTGATGACG CAATCTGCCT CGCAGAGTTG AAGTTGGGCT 
     2701 TCATAGCCCA GAGCTGCCTG GCTCAAGGCC TCTCCACCAT GCTTGCCAAC CTCTTCTCCA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CAAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTCAAGC TCCTAATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACCGAGA GAGCCGTATA TTAATTAATC CTGGAAACCA TCTTAAGATC CAAGAAGGTA 
     3001 CTTTAGGATT TTTCATCGCA AGTGATGCCA AAGAAGTTAA AAGGGCATTT TTTTACTGCA 
     3061 AGGCCTGTCA TGATGACATC ACAGATCCCA AAAGAATAAA AAAATGTGGC TGCAAACGGC 
     3121 TTGAAGATGA GCAGCCGTCA ACACTATCAC CAAAAAAAAA GCAACGGAAT GGAGGCATGC 
     3181 GGAACTCACC CAACACCTCG CCTAAGCTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA 
     3241 ATGATCAGAT TGACAACATG GACTCCAATG TGAAGAAGTA CGACTCTACT GGGATGTTTC 
     3301 ACTGGTGTGC ACCCAAGGAG ATAGAGAAAG TCATCCTGAC TCGAAGTGAA GCTGCCATGA 
     3361 CCGTCCTGAG TGGCCATGTC GTGGTCTGCA TCTTTGGCGA CGTCAGCTCA GCCCTGATCG 
     3421 GCCTCCGGAA CCTGGTGATG CCGCTCCGTG CCAGCAACTT TCATTACCAT GAGCTCAAGC 
     3481 ACATTGTGTT TGTGGGCTCT ATTGAGTACC TCAAGCGGGA ATGGGAGACG CTTCATAACT 
     3541 TCCCCAAAGT GTCCATATTG CCTGGTACGC CATTAAGTCG GGCTGATTTA AGGGCTGTCA 
     3601 ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT ATTGATGATA 
     3661 CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT ATGCAGTTTG 
     3721 ATGACAGCAT CGGAGTCTTG CAGGCTAATT CCCAAGGGTT CACACCTCCA GGAATGGATA 
     3781 GATCCTCTCC AGATAACAGC CCAGTGCACG GGATGTTACG TCAACCATCC ATCACAACTG 
     3841 GGGTCAACAT CCCCATCATC ACTGAACTAG TGAACGATAC TAATGTTCAG TTTTTGGACC 
     3901 AAGACGATGA TGATGACCCT GATACAGAAC TGTACCTCAC GCAGCCCTTT GCCTGTGGGA 
     3961 CAGCATTTGC CGTCAGTGTC CTGGACTCAC TCATGAGCGC GACGTACTTC AATGACAATA 
     4021 TCCTCACCCT GATACGGACC CTGGTGACCG GAGGAGCCAC GCCGGAGCTG GAGGCTCTGA 
     4081 TTGCTGAGGA AAACGCCCTT AGAGGTGGCT ACAGCACCCC GCAGACACTG GCCAATAGGG 
     4141 ACCGCTGCCG CGTGGCCCAG TTAGCTCTGC TCGATGGGCC ATTTGCGGAC TTAGGGGATG 
     4201 GTGGTTGTTA TGGTGATCTG TTCTGCAAAG CTCTGAAAAC ATATAATATG CTTTGTTTTG 
     4261 GAATTTACCG GCTGAGAGAT GCTCACCTCA GCACCCCCAG TCAGTGCACA AAGAGGTATG 
     4321 TCATCACCAA CCCGCCCTAT GAGTTTGAGC TCGTGCCGAC GGACCTGATC TTCTGCTTAA 
     4381 TGCAGTTTGA CCACAATGCC GGCCAGTCCC GGGCCAGCCT GTCCCATTCC TCCCACTCGT 
     4441 CGCAGTCCTC CAGCAAGAAG AGCTCCTCTG TTCACTCCAT CCCATCCACA GCAAACCGAC 
     4501 AGAACCGGCC CAAGTCCAGG GAGTCCCGGG ACAAACAGAA GTACGTGCAG GAAGAGCGGC 
     4561 TTAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4621 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4681 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4741 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4801 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4861 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4921 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4981 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5041 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5101 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5161 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5221 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5281 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5341 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5401 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5461 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5521 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5581 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5641 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5701 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5761 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5821 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5881 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5941 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6001 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6061 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6121 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6181 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6241 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6301 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6361 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6421 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6481 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6541 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6601 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6661 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6721 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6781 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6841 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6901 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6961 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7021 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7081 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7141 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7201 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7261 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7321 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7381 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7441 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7501 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7561 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7621 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7861 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7921 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7981 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8041 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8101 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8161 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8221 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8281 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8341 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8401 TAACAAAAAT TTAACGCGAA TTTT
//