LOCUS       RC215089                8307 bp    DNA     circular  SYN 
DEFINITION  ENAM (Myc-DDK-tagged)-Human enamelin (ENAM)
ACCESSION   NM_031889
VERSION     NM_031889.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4464..4555 
                     /label="Myc-DDK"  
     misc_feature    4585..5170 
                     /label="PolyA signal"  
     misc_feature    4642..4661 
                     /label="Restriction Enzyme"  
     misc_feature    5446..6118 
                     /label="ColE1"  
     misc_feature    6548..7342 
                     /label="Kan/Neo"  
     misc_feature    7377..7679 
                     /label="SV40 ori"  
     misc_feature    7765..8242 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4446..4452 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4446 
                     /label="ENAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGTGCTT CGGTGCAGGC TTGGAACCTC TTTTCCTAAA CTAGATAACT 
     1081 TGGTACCAAA AGGCAAAATG AAGATTCTCC TGGTCTTTCT AGGGCTTCTT GGTAATTCTG 
     1141 TTGCTATGCC AATGCACATG CCCCGAATGC CTGGATTTAG CAGTAAAAGT GAGGAGATGA 
     1201 TGCGGTATAA TCAATTCAAC TTTATGAACG GCCCACATAT GGCACACCTG GGGCCCTTCT 
     1261 TTGGAAACGG TCTCCCTCAG CAATTTCCAC AGTACCAGAT GCCCATGTGG CCTCAGCCAC 
     1321 CACCCAACAC ATGGCATCCA CGGAAATCCT CAGCACCCAA ACGTCATAAC AAGACTGATC 
     1381 AGACCCAAGA AACCCAGAAA CCCAACCAGA CTCAGTCAAA AAAGCCACCA CAAAAGCGGC 
     1441 CTTTGAAGCA GCCATCACAT AATCAACCTC AGCCCGAAGA GGAAGCTCAA CCCCCTCAGG 
     1501 CATTCCCACC ATTTGGAAAT GGGCTATTCC CCTATCAACA ACCACCATGG CAAATTCCAC 
     1561 AGAGGTTACC ACCACCAGGT TATGGACGCC CACCAATCAG CAATGAAGAA GGGGGGAATC 
     1621 CTTACTTTGG ATATTTTGGA TATCATGGCT TTGGGGGTCG CCCTCCTTAT TATTCAGAAG 
     1681 AAATGTTTGA ACAAGATTTT GAAAAACCCA AAGAAGAAGA TCCTCCTAAA GCAGAAAGTC 
     1741 CAGGCACAGA ACCCACAGCT AATTCAACAG TCACTGAGAC GAATTCTACC CAACCAAATC 
     1801 CTAAAGGGAG TCAGGGAGGA AATGACACCA GCCCCACAGG AAACAGTACC CCAGGACTAA 
     1861 ACACTGGGAA CAACCCTTCA GCTCGAAATG GGATTGGCCC ACTCCCTGCA GTCAACGCTT 
     1921 CAGGCCAGGG AGGGCCAGGA AGTCAAATCC CATGGAGACC AAGTCAGCCA AATATTCGTG 
     1981 AAAATCATCC ATATCCTAAT ATAAGAAATT TTCCTTCAGG AAGACAGTGG TATTTCACTG 
     2041 GTACTGTCAT GGGGCACAGA CAGAATAGGC CTTTTTACAG AAATCAACAA GTTCAAAGGG 
     2101 GTCCTCGGTG GAACTTCTTT GCTTGGGAAC GTAAACAAGT AGCTCGTCCA GGAAATCCAG 
     2161 TTTATCACAA AGCTTACCCT CCTACTTCAA GAGGCAATTA TCCCAATTAT GCAGGAAATC 
     2221 CAGCAAATCT CAGAAGAAAG CCTCAGGGGC CAAATAAACA CCCTGTAGGA ACTACTGTTG 
     2281 CCCCACTGGG TCCCAAACCT GGCCCTGTTG TTCGCAATGA AAAAATCCAA AATCCAAAGG 
     2341 AGAAGCCCCT GGGTCCAAAA GAACAAATAA TAGTTCCTAC AAAGAATCCA ACCAGCCCCT 
     2401 GGAGAAACTC TCAACAGTAT GAAGTTAATA AATCAAATTA TAAACTGCCT CACTCTGAGG 
     2461 GTTATATGCC AGTCCCAAAT TTTAATTCTG TTGATCAACA TGAAAACTCC TATTACCCAA 
     2521 GAGGAGATTC CAGAAAAGTC CCAAATTCTG ATGGACAAAC CCAAAGCCAG AATTTGCCCA 
     2581 AAGGGATTGT TTTAGGGTCA AGAAGGATGC CATATGAATC AGAAACTAAT CAGTCAGAAT 
     2641 TAAAGCACAG CTCATATCAG CCTGCTGTAT ACCCTGAGGA AATCCCTTCT CCTGCAAAAG 
     2701 AACATTTTCC TGCTGGAAGA AATACTTGGG ACCACCAAGA AATCTCTCCA CCTTTTAAGG 
     2761 AAGATCCAGG GAGGCAAGAA GAACATTTAC CCCATCCTTC CCATGGTTCT AGAGGAAGTG 
     2821 TTTTCTACCC TGAATATAAC CCATATGATC CCAGGGAAAA CTCACCATAC CTTAGAGGCA 
     2881 ATACATGGGA TGAGAGAGAT GATTCTCCCA ATACTATGGG GCAAAAAGAA AGTCCACTCT 
     2941 ACCCCATAAA TACCCCAGAC CAGAAGGAGA TAGTCCCTTA TAATGAAGAG GACCCAGTTG 
     3001 ATCCAACTGG AGATGAAGTC TTTCCTGGAC AAAATAGATG GGGTGAAGAG TTGAGCTTCA 
     3061 AAGGAGGCCC AACAGTTAGG CACTATGAAG GTGAACAATA TACCTCAAAT CAGCCAAAGG 
     3121 AATATCTTCC CTATTCTTTA GATAATCCAT CAAAACCAAG GGAGGATTTT TATTACAGTG 
     3181 AATTTTACCC ATGGAGCCCG GATGAGAATT TTCCATCATA TAATACAGCT TCTACTATGC 
     3241 CACCACCTAT AGAGAGCAGG GGCTACTACG TTAATAATGC CGCTGGACCA GAAGAAAGCA 
     3301 CTCTATTTCC TTCACGGAAT TCCTGGGACC ACAGGATACA AGCCCAAGGG CAGAGAGAAA 
     3361 GAAGGCCGTA TTTTAACAGA AATATCTGGG ATCAGGCAAC ACATTTACAA AAAGCCCCAG 
     3421 CTAGGCCACC AGACCAGAAA GGTAACCAGC CCTATTACAG TAACACCCCA GCTGGGCTTC 
     3481 AGAAAAATCC AATATGGCAT GAAGGTGAGA ATTTGAACTA TGGCATGCAA ATAACTAGGA 
     3541 TGAATTCTCC AGAGAGAGAA CATTCATCTT TCCCTAACTT CATCCCACCA AGTTACCCAT 
     3601 CAGGTCAAAA AGAAGCACAT TTATTTCACC TAAGCCAGAG AGGCTCTTGC TGTGCTGGTA 
     3661 GCTCCACAGG GCCCAAGGAC AATCCACTAG CTCTACAAGA CTACACTCCA TCCTATGGTC 
     3721 TTGCACCTGG GGAGAACCAA GACACCAGTC CTCTGTATAC AGACGGTAGT CATACCAAGC 
     3781 AGACAAGAGA TATCATCTCC CCAACAAGCA TCCTACCAGG CCAAAGAAAC AGCTCAGAGA 
     3841 AGAGGGAAAG CCAAAACCCT TTTAGAGATG GTGTGTCCAC GCTGAGGAGG AACACACCAT 
     3901 GTTCTATAAA GAATCAACTG GGCCAAAAGG AAATTATGCC CTTTCCTGAA GCCAGTTCCC 
     3961 TTCAATCAAA GAATACACCT TGTCTCAAAA ATGATCTTGG AGGAGATGGG AACAACATTC 
     4021 TGGAACAAGT TTTTGAAGAC AACCAGCTCA ATGAAAGAAC TGTTGACCTT ACTCCTGAGC 
     4081 AGCTTGTTAT TGGTACACCT GATGAAGGCT CCAATCCAGA AGGCATCCAA AGTCAAGTCC 
     4141 AAGAAAATGA GAGTGAGAGG CAACAGCAAA GACCATCTAA CATTCTGCAT TTGCCATGCT 
     4201 TTGGCTCCAA ATTAGCAAAG CATCACTCTT CCACCACCGG AACTCCATCT AGCGATGGAA 
     4261 GGCAAAGCCC ATTTGATGGG GATTCAATTA CGCCTACTGA AAATCCTAAC ACATTGGTTG 
     4321 AGTTAGCTAC TGAGGAACAA TTTAAGAGTA TAAATGTAGA CCCACTTGAT GCAGATGAAC 
     4381 ACAGTCCATT TGAATTCCTT CAAAGAGGGA CCAATGTACA GGACCAGGTA CAAGACTGCT 
     4441 TACTACTTCA GGCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4501 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4561 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4621 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4681 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4741 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4801 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4861 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4921 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4981 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5041 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5101 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5161 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5221 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5281 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5341 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5401 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5461 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5521 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5581 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5641 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5701 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5761 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5821 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5881 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5941 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6001 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6061 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6121 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6181 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6241 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6301 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6361 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6421 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6481 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6541 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6601 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6661 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6721 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6781 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6841 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6901 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6961 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7021 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7081 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7141 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7201 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7261 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7321 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7381 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7441 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7501 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7741 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7801 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7861 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7921 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7981 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8041 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8101 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8161 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8221 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8281 ATTTAACAAA AATTTAACGC GAATTTT
//