LOCUS       RC215077                8868 bp    DNA     circular  SYN 
DEFINITION  IGSF22 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 22 (IGSF22)
ACCESSION   NM_173588
VERSION     NM_173588.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5023..5114 
                     /label="Myc-DDK"  
     misc_feature    5144..5729 
                     /label="PolyA signal"  
     misc_feature    5201..5220 
                     /label="Restriction Enzyme"  
     misc_feature    6005..6677 
                     /label="ColE1"  
     misc_feature    7107..7901 
                     /label="Kan/Neo"  
     misc_feature    7936..8238 
                     /label="SV40 ori"  
     misc_feature    8324..8801 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4998..5005 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4998 
                     /label="IGSF22"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAACCATT CACAGCCGGC AGATGCTGCA GGAGCACGTG TCCATGGAGT 
     1081 TCTCCAGCTC CACCACCCAC GTGCAGACCT TCTCCCAGAC AACCAAGATC GTGGGAGAGG 
     1141 AGGTCGTGAG GAGGAAGTCC TCGAGCATAG TGGAGTTCTT CAGCTTAGTG ACCCGGAGCT 
     1201 CAAACATCCC TGCGGGCGAC AGCGTCCCTG AGTTCGTGGA GAAGCCTCAA CCGGTCACCG 
     1261 CGCCCGAGGG GGACAAAGCC GTGTTCCGAG CCCGGGTGCA GGGGAACGCC AAACCCCACA 
     1321 TCTCCTGGAA GAGGGAGAGC GGCATCCCCA TCAAGGAGTC CGCCAAGATA TTCTACGACA 
     1381 GCATTAACAA GGAACACGTG CTGAAGCTGG AGCCACTGAC TTCGGATGAC TCTGACAACT 
     1441 ATAAGTGCAT TGCAAGCAAT GACCATGCAG ATGCCATCTA TACCGTATCT CTGCTGGTAA 
     1501 CAGAAGGTCA AGAGAAAATG GACTTCAAAA AGATGCTGAA GAAGAGGGCA CCCCCTGCTC 
     1561 CCAAGAAGAA GCAGAAGAAG GTGGCAAATG AGAAAGAGAT GCTGGAGATT TTGTCCAAAG 
     1621 TGCCCAAGAA AGACTTTGAG AAGGTGTGCA TGGAGTATGG TTTCACCGAC TTTCGGGGGC 
     1681 TGCTCAGGAA GCTCAAAGAG ATGAAGAAGA AAGTAGAGGT GGAGGCCATC CGGATTCTGA 
     1741 AGCCCCTGGA AGACAAAGAG ACCAAGGTTG ACACCACAGT GGTCTTTGAC TGCATAATGG 
     1801 AACTGAAAGA CCCCAATGTC AAGATGATAT GGATCAAGGG TACTGAGCCA CTGAGGATCC 
     1861 AGTACTCCCT GGGCAAGTAC GATGTGAAGC AGATGGGCAC CAAGTACATG CTGGTTATTA 
     1921 GCAACGTGAA CATGAACGAT GCTGGCATCT ACAGCCTGTC CGTGGGCGAT AAGCGGATGA 
     1981 GTGCAGAGCT CACAGTGCTG GATGAGCCAC TGAAGTTCCT GGGAGAGATG AAGCCTGTGA 
     2041 AGGTGACAGA GCGCCAGACA GCTGTGTTTG AGATCCGCCT CTCCAAGAAA GAGCCCAACT 
     2101 TTGTGTGGAA GTTCAATGGG AAGGAGCTGA AGAGGGATGA CAAGTATGAA ATCACGGTGT 
     2161 CCGAAGATGG TCTGACGCAC ACGCTTAAGA TTAAGGATGC CAGACTCAGT GACAGCGGCG 
     2221 AGTTCTCTGC TGAGGCGGGG AACCTGGTAC AAAAGGCCCA GCTCACTATT GACCGCATCC 
     2281 CCATCAAGTT TGTGAGCAAC CTCAAAAATG TACGTGTGAA AGAGAGGAGT CGCGCATGCC 
     2341 TGGAGTGTGA GCTGACATCC AAGGATGTGA CACTGCGCTG GAAGAAGGAT GGGCAGCTGC 
     2401 TGATGCATGG CACTAAGTAC AGCATGAACC ATGAGGGCAA GCGAGCAGAG CTGATCATTG 
     2461 AGGATGCACA GCTCAGTGAT GGTGGCGAGT ACACTGTGGT GGCCATGCAG GATGGAGACC 
     2521 CTACTGAATA CTACAGTACT GCCATCGTCA CTGTGGAGGA GCGTCTGGCC ACAGTGAAGA 
     2581 GCGGGATGTC CGACGTGCAC GCGGCCACTG GGAGCCCAGC TGAGTTGTGT GTAGTGCTGA 
     2641 ATGACGAGAA GGTGGAGGGT GTGTGGCTGA AGGATGGCAA GGAGATCACG GACTTGCCAG 
     2701 GCATGCAGAT TGTGAAGCAG GGTGCAGTGC ACAAGCTCAT CTTTCCCAGT ATGGGCCCTG 
     2761 AGCACGAGGG CAAGTACACA TTCCGGGCCA AGGGCACGGA AAGTGAAGCC TCTGTATTCA 
     2821 TCGCAGATCC TCCTACCATC GACCCGTCAG TACTGGAGGC ACTGGCTGCG CACGCCATCA 
     2881 CTGTGAAGGT AGGCCACACG GCCCACATCA AGGTCCCCTT CCGGGGAAAA CCACTGCCCA 
     2941 AAGTGACATG GTACAAGGAT GGCATGGAAG TGACGGAGGA GGAACGCGTG TCCATGGAGC 
     3001 GCGGGGAAGA CCAGGCACTG CTCACCATCT CCAACTGTGT GCGTGAAGAC AGCGGCCTCA 
     3061 TCCTGCTCAA GCTCAAGAAT GACCACGGCT CAGCCACGGC CACTCTGCAC CTTAGTGTGC 
     3121 TGGACCGTCC AAAGCCTCCA CAGGGCCGGG TGGAGTTCCT GGAGCTCTCA GGTAGTTGTG 
     3181 TGCACATGAA GTGGAAGGCC CCAAAGGACA ATGGTGGACG ACCTGTGACA CAGTTCATAG 
     3241 TGGAACGGAG GGCAGTTGGC AAGAAGTCCT GGATTAAGAT AGGCGAGGTG GACGGCAAAG 
     3301 TCACCAACTT CTCCACCAAC AAGGTGGAAG AGGGAAAAGC CTACCAGTTC CGTATCCTGG 
     3361 CAGTCAATTC AGAAGGTGTG AGTGACCCAC TGGAGACAGA AGAAGTGTTT GCAGGAAATC 
     3421 CCATAGAGCC TCCTGGTTTT GCCTCCCAGC CTCAAGTGAC TGATGTGACT AAAGAAGCCG 
     3481 TGACCATCAC GTGGAATGCC CCTACCCAGG ATGGGGGAGC CCCAGTGCTC GGCTACATTG 
     3541 TAGAACGAAG GAAGAAAGGC AGCAACCTGT GGGTGCCAGT CAACAAGGAC CCCATCCAGG 
     3601 GCACCAAGTG CACTGTGGAT GGTCTCCTGG AGGACACAGA ATATGAATTC CGAGTTATAG 
     3661 CTGTAAATAA GGCAGGCCCT GGACAGCCCA GTGTGCCATC CAGCTCAGTA GTGGCCAAGG 
     3721 ATCCTGTTAA ACCCCCAGGC CTGGTCCAGG ACCTGCATGT ATCTGATTCC TCCAACTCCA 
     3781 GCATTTCCCT GGCCTGGCGG GAGCCTGCAG AGGGAGACCC ACCCTCTGGC TACATCCTTG 
     3841 AGATGAGGGC TGAAGACACA AAGGAGTGGT CCAAGTGCAC AAAGATCCCC ATCTCAGGCA 
     3901 CCTGCTACAC AGTGGGAGGA CTCATCGAGA GGCAGAAATA CTTCTTCCGA ATCCGGGCTG 
     3961 TGAATGAGGC TGGGGTTGGG GAGCCTGTGG AGCTAGACAA GGGGGTCCGT GCCATGCCAC 
     4021 CACCAGCTGC ACCCAAGTTT GACCTCAGTG CCCGGCTGAA GAGTCACATG GTGGTTCGCG 
     4081 CTGGGACAGC CCTCTGCATC CATGCAGCCT TCTCTGGCTC ACCACCACCT GACGTGATCT 
     4141 GGCAGAAAGA TGGCGTTCCC ACCAAGGGCC GAGAGACAAT TACCAAGAGC AAAAACCACT 
     4201 CCCAGTTCCT CATTAATAGC ACCAAGCGCT CTGACTCAGG GGTGTACCGA ATCTTGCTTC 
     4261 AGAATGAGTT TGGAGAAGCA CGCTATGACA TCCATGTGCG CGTGGCAGAT TTCCCTCGGC 
     4321 CCCCCACAAA CCTACGGTTG TTTGAGGAAG TCCCCAACAC AGTGACTCTG ACCTGGAACC 
     4381 ACAGCCCAGA TGTGCAGGAG GACGGTGAGG CTCACTACAT CATCATGAAG CGGGATGCAA 
     4441 GCACAGCCAC CTGGTACACG GCAGCCGAGC GTGTCTTCAG CAACAAGTAC ACAGTGACGG 
     4501 GGCTGCTCCC AGGCAGGAAG TACTACTTCA GAGTGGTGGC TCGGAATGAA ATCGGTGACA 
     4561 GTGAGCCACT TGACTCCAGG GACACCTGGC TCATCAATAA GGACCAGATC CAGGACCTGA 
     4621 GCGCCAAGCT CAAGCCCTAC GAGAAGAAGG ACTGGCGCCA CGCGCCGCGC TTCGTGACGC 
     4681 CCCTCAAGCC ACACACGGTG CTCCGCGGCC AGGACTGCAC CATGACCTGC GCCTTCCTTG 
     4741 GGAACCCGCG GCCCACAGTG ACCCTCTACA AGGGCGACGT CAACATCACG GCCAACTCCA 
     4801 AGTTCTGGTA CAACTCCACC AGCGGCGTGT GCACCCTCGT CATCCCCACC TGCACGCTCA 
     4861 AGGACAGCGG CGATTACAGC GTGCTGGTGG AGAACGAGCT GGGCAAGGAC CGCAGCAGCT 
     4921 GCACGCTCAC CGTCTATGAC AAAGATGATA AGTCAGTTGT AGCATCCATC ACCGAGAGTC 
     4981 TGCAGAAGAA ATCAAAGCAC CTCATGAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     5041 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5101 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5161 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5221 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5281 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5341 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5401 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5461 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5521 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5581 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5641 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5701 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5761 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5821 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5881 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5941 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6001 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6061 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6121 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6181 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6241 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6301 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6361 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6421 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6481 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6541 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6601 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6661 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6721 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6781 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6841 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6901 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6961 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7021 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7081 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7141 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7201 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7261 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7321 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7381 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7441 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7501 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7561 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7621 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7681 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7741 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7801 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7861 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7921 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7981 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8041 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8101 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8161 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8221 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8281 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8341 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8401 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8461 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8521 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8581 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8641 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8701 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8761 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8821 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//