LOCUS       RC215065                7830 bp    DNA     circular  SYN 
DEFINITION  NLRP7 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 2
ACCESSION   NM_206828
VERSION     NM_206828.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3985..4076 
                     /label="Myc-DDK"  
     misc_feature    4106..4691 
                     /label="PolyA signal"  
     misc_feature    4163..4182 
                     /label="Restriction Enzyme"  
     misc_feature    4967..5639 
                     /label="ColE1"  
     misc_feature    6069..6863 
                     /label="Kan/Neo"  
     misc_feature    6898..7200 
                     /label="SV40 ori"  
     misc_feature    7286..7763 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3960..3967 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3960 
                     /label="NLRP7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACATCGCCC CAGCTAGAGT GGACTCTGCA GACCCTTCTG GAGCAGCTGA 
     1081 ACGAGGATGA ATTAAAGAGT TTCAAATCCC TTTTATGGGC TTTTCCCCTC GAAGACGTGC 
     1141 TACAGAAGAC CCCATGGTCT GAGGTGGAAG AGGCTGATGG CAAGAAACTG GCAGAAATTC 
     1201 TGGTCAACAC CTCCTCAGAA AATTGGATAA GGAATGCGAC TGTGAACATC TTGGAAGAGA 
     1261 TGAATCTCAC GGAATTGTGT AAGATGGCAA AGGCTGAGAT GATGGAGGAC GGACAGGTGC 
     1321 AAGAAATAGA TAATCCTGAG CTGGGAGATG CAGAAGAAGA CTCGGAGTTA GCAAAGCCAG 
     1381 GTGAAAAGGA AGGATGGAGA AATTCAATGG AGAAACAGTC TTTGGTCTGG AAGAACACCT 
     1441 TTTGGCAAGG AGACATTGAC AATTTCCATG ACGACGTCAC TCTGAGAAAC CAACGGTTCA 
     1501 TTCCATTCTT GAATCCCAGA ACACCCAGGA AGCTAACACC TTACACGGTG GTGCTGCACG 
     1561 GCCCCGCAGG CGTGGGGAAA ACCACGCTGG CCAAAAAGTG TATGCTGGAC TGGACAGACT 
     1621 GCAACCTCAG CCCGACGCTC AGATACGCGT TCTACCTCAG CTGCAAGGAG CTCAGCCGCA 
     1681 TGGGCCCCTG CAGTTTTGCA GAGCTGATCT CCAAAGACTG GCCTGAATTG CAGGATGACA 
     1741 TTCCAAGCAT CCTAGCCCAA GCACAGAGAA TCCTGTTCGT GGTCGATGGC CTTGATGAGC 
     1801 TGAAAGTCCC ACCTGGGGCG CTGATCCAGG ACATCTGCGG GGACTGGGAG AAGAAGAAGC 
     1861 CGGTGCCCGT CCTCCTGGGG AGTTTGCTGA AGAGGAAGAT GTTACCCAGG GCAGCCTTGC 
     1921 TGGTCACCAC GCGGCCCAGG GCACTGAGGG ACCTCCAGCT CCTGGCGCAG CAGCCGATCT 
     1981 ACGTAAGGGT GGAGGGCTTC CTGGAGGAGG ACAGGAGGGC CTATTTCCTG AGACACTTTG 
     2041 GAGACGAGGA CCAAGCCATG CGTGCCTTTG AGCTAATGAG GAGCAACGCG GCCCTGTTCC 
     2101 AGCTGGGCTC GGCCCCCGCG GTGTGCTGGA TTGTGTGCAC GACTCTGAAG CTGCAGATGG 
     2161 AGAAGGGGGA GGACCCGGTC CCCACCTGCC TCACCCGCAC GGGGCTGTTC CTGCGTTTCC 
     2221 TCTGCAGCCG GTTCCCGCAG GGCGCACAGC TGCGGGGCGC GCTGCGGACG CTGAGCCTCC 
     2281 TGGCCGCGCA GGGCCTGTGG GCGCAGATGT CCGTGTTCCA CCGAGAGGAC CTGGAAAGGC 
     2341 TCGGGGTGCA GGAGTCCGAC CTCCGTCTGT TCCTGGACGG AGACATCCTC CGCCAGGACA 
     2401 GAGTCTCCAA AGGCTGCTAC TCCTTCATCC ACCTCAGCTT CCAGCAGTTT CTCACTGCCC 
     2461 TGTTCTACGC CCTGGAGAAG GAGGAGGGGG AGGACAGGGA CGGCCACGCC TGGGACATCG 
     2521 GGGACGTACA GAAGCTGCTT TCCGGAGAAG AAAGACTCAA GAACCCCGAC CTGATTCAAG 
     2581 TAGGACACTT CTTATTCGGC CTCGCTAACG AGAAGAGAGC CAAGGAGTTG GAGGCCACTT 
     2641 TTGGCTGCCG GATGTCACCG GACATCAAAC AGGAATTGCT GCAATGCAAA GCACATCTTC 
     2701 ATGCAAATAA GCCCTTATCC GTGACCGACC TGAAGGAGGT CTTGGGCTGC CTGTATGAGT 
     2761 CTCAGGAGGA GGAGCTGGCG AAGGTGGTGG TGGCCCCGTT CAAGGAAATT TCTATTCACC 
     2821 TGACAAATAC TTCTGAAGTG ATGCATTGTT CCTTCAGCCT GAAGCATTGT CAAGACTTGC 
     2881 AGAAACTCTC ACTGCAGGTA GCAAAGGGGG TGTTCCTGGA GAATTACATG GATTTTGAAC 
     2941 TGGACATTGA ATTTGAAAGG TGCACTTACC TAACCATTCC GAACTGGGCT CGGCAGGATC 
     3001 TTCGCTCTCT TCGCCTCTGG ACAGATTTCT GCTCTCTCTT CAGCTCAAAC AGCAACCTCA 
     3061 AGTTTCTGGA AGTGAAACAA AGCTTCCTGA GTGACTCTTC TGTGCGGATT CTTTGTGACC 
     3121 ACGTAACCCG TAGCACCTGT CATCTGCAGA AAGTGGAGAT TAAAAACGTC ACCCCTGACA 
     3181 CCGCGTACCG GGACTTCTGT CTTGCTTTCA TTGGGAAGAA GACCCTCACG CACCTGACCC 
     3241 TGGCAGGGCA CATCGAGTGG GAACGCACGA TGATGCTGAT GCTGTGTGAC CTGCTCAGAA 
     3301 ATCATAAATG CAACCTGCAG TACCTGAGGT TGGGAGGTCA CTGTGCCACC CCGGAGCAGT 
     3361 GGGCTGAATT CTTCTATGTC CTCAAAGCCA ACCAGTCCCT GAAGCACCTG CGTCTCTCAG 
     3421 CCAATGTGCT CCTGGATGAG GGTGCCATGT TGCTGTACAA GACCATGACA CGCCCAAAAC 
     3481 ACTTCCTGCA GATGTTGTCG TTGGAAAACT GTCGTCTTAC AGAAGCCAGT TGCAAGGACC 
     3541 TTGCTGCTGT CTTGGTTGTC AGCAAGAAGC TGACACACCT GTGCTTGGCC AAGAACCCCA 
     3601 TTGGGGATAC AGGGGTGAAG TTTCTGTGTG AGGGCTTGAG TTACCCTGAT TGTAAACTGC 
     3661 AGACCTTGGT GTTACAGCAA TGCAGCATAA CCAAGCTTGG CTGTAGATAT CTCTCAGAGG 
     3721 CGCTCCAAGA AGCCTGCAGC CTCACAAACC TGGACTTGAG TATCAACCAG ATAGCTCGTG 
     3781 GATTGTGGAT TCTCTGTCAG GCATTAGAGA ATCCAAACTG TAACCTAAAA CACCTACGGT 
     3841 TGAAGACCTA TGAAACTAAT TTGGAAATCA AGAAGCTGTT GGAGGAAGTG AAAGAAAAGA 
     3901 ATCCCAAGCT GACTATTGAT TGCAATGCTT CCGGGGCAAC GGCACCTCCG TGCTGTGACT 
     3961 TTTTTTGCAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4021 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4081 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4141 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4201 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4261 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4321 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4381 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4441 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4501 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4561 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4621 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4681 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4741 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4801 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4861 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4921 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4981 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5041 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5101 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5161 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5221 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5281 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5341 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5401 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5461 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5521 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5581 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5641 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5701 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5761 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5821 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5881 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5941 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6001 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6061 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6121 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6181 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6241 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6301 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6361 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6421 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6481 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6541 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6601 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6661 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6721 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6781 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6841 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6901 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6961 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7021 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7081 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7141 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7201 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7261 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7321 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7381 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7441 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7501 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7561 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7621 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7681 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7741 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7801 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//