LOCUS       RC215055                8304 bp    DNA     circular  SYN 
DEFINITION  SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 5
ACCESSION   NM_001042496
VERSION     NM_001042496.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4461..4552 
                     /label="Myc-DDK"  
     misc_feature    4582..5167 
                     /label="PolyA signal"  
     misc_feature    4639..4658 
                     /label="Restriction Enzyme"  
     misc_feature    5443..6115 
                     /label="ColE1"  
     misc_feature    6545..7339 
                     /label="Kan/Neo"  
     misc_feature    7374..7676 
                     /label="SV40 ori"  
     misc_feature    7762..8239 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4443..4449 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4443 
                     /label="SLC12A6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCAGTT CGGTTCATGG TGACACCGAC AAAGATCGAT GACATTCCAG 
     1081 GTTTGTCAGA CACCAGTCCG GACCTCAGCT CTCGATCTAG TTCCCGAGTA AGATTTAGCT 
     1141 CCCGGGAAAG CGTGCCTGAA ACAAGCCGGA GTGAGCCTAT GAGTGAGATG TCTGGGGCCA 
     1201 CCACTTCGCT GGCAACTGTT GCACTGGATC CACCCAGTGA CCGGACTTCT CACCCCCAGG 
     1261 ATGTCATCGA GGACCTGAGT CAGAACTCCA TCACAGGGGA ACACAGCCAA CTGTTAGACG 
     1321 ACGGACATAA GAAAGCTCGA AATGCTTATC TCAATAATTC CAATTATGAA GAAGGAGATG 
     1381 AATATTTTGA TAAAAATTTG GCACTCTTTG AGGAAGAAAT GGACACCAGA CCGAAGGTGT 
     1441 CTTCCCTCCT CAACCGCATG GCCAATTACA CTAATCTGAC TCAAGGAGCA AAGGAACATG 
     1501 AAGAGGCAGA AAACATCACT GAAGGGAAAA AGAAGCCCAC CAAGACCCCC CAAATGGGTA 
     1561 CCTTCATGGG TGTCTACCTC CCATGTCTAC AAAATATTTT TGGAGTGATC CTTTTTTTAC 
     1621 GCCTTACATG GGTGGTGGGC ACAGCTGGAG TTCTTCAGGC TTTTGCAATT GTCCTTATCT 
     1681 GCTGCTGCTG TACAATGTTG ACTGCTATCT CCATGAGTGC CATTGCCACT AATGGAGTGG 
     1741 TGCCAGCTGG GGGCTCATAC TTTATGATTT CCCGGGCACT GGGCCCAGAG TTTGGTGGGG 
     1801 CTGTTGGCCT CTGCTTTTAT CTTGGTACCA CATTTGCAGC AGCCATGTAC ATCCTTGGTG 
     1861 CCATTGAAAT CTTTCTGGTC TATATCGTCC CCCGAGCTGC CATCTTTCAC AGTGATGACG 
     1921 CACTCAAGGA ATCAGCAGCC ATGCTAAATA ACATGCGTGT CTACGGCACA GCTTTCTTGG 
     1981 TCCTTATGGT ATTAGTGGTA TTTATCGGCG TACGCTATGT GAACAAGTTT GCCTCACTTT 
     2041 TCCTGGCCTG TGTCATTGTG TCCATCTTGG CCATCTATGC TGGAGCCATC AAGTCTTCTT 
     2101 TTGCTCCTCC ACACTTCCCG GTCTGCATGC TGGGTAACCG CACCCTTTCA TCAAGACACA 
     2161 TTGACGTTTG CTCTAAGACC AAGGAAATTA ACAACATGAC AGTCCCATCA AAGTTATGGG 
     2221 GATTCTTCTG TAACTCGAGT CAATTTTTCA ATGCCACCTG TGATGAATAC TTTGTTCACA 
     2281 ATAACGTCAC TTCAATCCAG GGCATTCCTG GATTGGCTAG TGGTATAATT ACAGAGAATC 
     2341 TTTGGAGTAA TTACCTACCC AAGGGAGAGA TCATCGAAAA GCCTTCAGCC AAATCTTCTG 
     2401 ATGTCTTAGG CAGCTTAAAC CATGAATATG TTCTTGTTGA CATCACCACC TCCTTCACGC 
     2461 TTCTGGTGGG AATCTTCTTT CCCTCTGTTA CAGGTATCAT GGCTGGATCA AACAGATCTG 
     2521 GAGATCTGAA AGATGCTCAG AAGTCTATTC CGATTGGTAC TATCCTTGCC ATCCTGACCA 
     2581 CCTCCTTTGT TTATTTAAGC AATGTTGTCC TTTTTGGTGC ATGTATTGAA GGGGTTGTTC 
     2641 TCAGAGACAA GTTCGGTGAT GCTGTGAAAG GTAATTTGGT GGTAGGCACC TTATCTTGGC 
     2701 CATCCCCATG GGTGATTGTT ATTGGCTCCT TCTTTTCAAC ATGTGGGGCT GGACTTCAGA 
     2761 GCCTCACAGG TGCACCGAGG CTGCTACAAG CTATTGCCAA GGATAACATC ATACCGTTTC 
     2821 TGAGGGTTTT TGGCCACAGC AAAGCCAATG GGGAACCTAC CTGGGCTTTA CTTCTAACTG 
     2881 CTGCCATTGC AGAGCTTGGA ATACTCATTG CCTCCCTGGA TCTTGTGGCC CCAATTCTTT 
     2941 CCATGTTTTT TCTCATGTGT TACCTCTTTG TAAACTTGGC ATGTGCCTTG CAAACATTAC 
     3001 TTCGAACACC CAACTGGAGA CCCCGATTCC GCTACTACCA TTGGGCCCTT TCTTTCATGG 
     3061 GAATGAGTAT CTGTCTGGCT CTGATGTTCA TTTCTTCCTG GTATTATGCC ATTGTAGCCA 
     3121 TGGTAATAGC TGGTATGATC TACAAGTACA TTGAATACCA AGGAGCTGAG AAAGAATGGG 
     3181 GTGATGGTAT CCGTGGGCTG TCCCTCAGTG CAGCCCGGTT TGCTTTGCTT CGATTGGAGG 
     3241 AAGGACCTCC ACACACTAAA AACTGGAGGC CTCAGTTGCT TGTATTACTG AAACTAGATG 
     3301 AAGACTTACA TGTCAAGCAT CCTCGCCTCC TCACCTTTGC CTCACAGCTC AAAGCAGGAA 
     3361 AAGGTCTCAC TATTGTGGGC TCTGTCATCG TGGGGAACTT CCTAGAGAAC TACGGTGAAG 
     3421 CTTTAGCTGC TGAGCAGACC ATAAAGCACC TAATGGAGGC AGAGAAGGTA AAAGGATTCT 
     3481 GCCAGCTGGT GGTGGCCGCC AAGCTGAGAG AGGGCATTTC CCACCTCATC CAGTCATGTG 
     3541 GCCTTGGGGG CATGAAGCAC AACACGGTGG TGATGGGCTG GCCTAATGGC TGGCGTCAAA 
     3601 GCGAAGATGC CCGCGCTTGG AAGACTTTTA TTGGCACAGT TCGAGTGACA ACTGCTGCCC 
     3661 ATCTTGCACT GCTGGTGGCT AAAAACATCT CCTTCTTTCC CAGCAATGTG GAGCAATTTT 
     3721 CTGAGGGCAA CATTGATGTG TGGTGGATTG TGCATGATGG GGGGATGCTT ATGCTACTAC 
     3781 CATTCCTACT GAAACAGCAC AAGGTGTGGC GAAAGTGCAG CATACGGATC TTCACAGTAG 
     3841 CCCAATTAGA AGACAACAGT ATCCAAATGA AGAAGGACCT AGCCACCTTC CTATATCACT 
     3901 TACGCATTGA GGCGGAGGTA GAAGTGGTGG AGATGCATGA CAGTGATATA TCAGCATATA 
     3961 CTTACGAGCG CACTTTGATG ATGGAACAAA GGTCCCAGAT GCTCCGGCAC ATGCGGCTAT 
     4021 CCAAAACAGA GCGAGACAGA GAGGCACAAT TGGTGAAAGA CCGAAACTCA ATGCTACGAT 
     4081 TGACCAGCAT TGGCTCTGAT GAGGACGAAG AGACAGAAAC CTATCAGGAG AAGGTGCACA 
     4141 TGACTTGGAC AAAAGACAAG TACATGGCAT CCCGGGGACA AAAAGCGAAG TCAATGGAAG 
     4201 GATTCCAGGA CCTGCTTAAC ATGCGTCCGG ACCAGTCCAA TGTGAGGCGG ATGCATACAG 
     4261 CAGTGAAACT CAACGAGGTT ATAGTTAACA AGTCCCATGA AGCAAAGCTG GTTTTATTGA 
     4321 ATATGCCAGG GCCACCCCGA AACCCTGAGG GTGATGAAAA CTACATGGAG TTCCTAGAGG 
     4381 TGCTTACCGA GGGACTAGAG CGAGTCCTAC TTGTCCGGGG TGGTGGCAGT GAAGTGATCA 
     4441 CCATTTATTC AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4501 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4561 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4621 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4681 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4741 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4801 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4861 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4921 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4981 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5041 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5101 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5161 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5221 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5281 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5341 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5401 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5461 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5521 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5581 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5641 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5701 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5761 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5821 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5881 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5941 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6001 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6061 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6121 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6181 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6241 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6301 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6361 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6421 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6481 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6541 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6601 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6661 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6721 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6781 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6841 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6901 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6961 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7021 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7081 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7141 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7201 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7261 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7321 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7381 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7441 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7501 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7741 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7801 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7861 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7921 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7981 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8041 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8101 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8161 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8221 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8281 TAACAAAAAT TTAACGCGAA TTTT
//