LOCUS       RC215038                8853 bp    DNA     circular  SYN 
DEFINITION  NFRKB (Myc-DDK-tagged)-Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 2
ACCESSION   NM_006165
VERSION     NM_006165.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5010..5101 
                     /label="Myc-DDK"  
     misc_feature    5131..5716 
                     /label="PolyA signal"  
     misc_feature    5188..5207 
                     /label="Restriction Enzyme"  
     misc_feature    5992..6664 
                     /label="ColE1"  
     misc_feature    7094..7888 
                     /label="Kan/Neo"  
     misc_feature    7923..8225 
                     /label="SV40 ori"  
     misc_feature    8311..8788 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4992..4998 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4992 
                     /label="NFRKB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATGTCTTT GTCCAGAGTA CCTGTGGAGA AGAAACAATG GATTCCTTAG 
     1081 ACCATATGCT GACAGATCCT CTGGAACTTG GTCCGTGTGG AGATGGCCAT GGCACGCGCA 
     1141 TCATGGAGGA TTGCCTCCTG GGAGGCACCA GAGTTAGTCT GCCCGAGGAC CTTCTGGAGG 
     1201 ATCCTGAGAT CTTCTTTGAT GTTGTCAGCC TCTCAACATG GCAGGAAGTG TTAAGTGATT 
     1261 CTCAACGTGA ACACCTCCAG CAGTTTCTGC CCCAGTTTCC TGAAGACAGT GCTGAGCAGC 
     1321 AGAATGAACT CATCTTAGCC TTGTTCAGTG GGGAGAACTT CCGCTTTGGA AACCCTCTGC 
     1381 ACATTGCCCA GAAGCTTTTC CGAGACGGAC ACTTTAACCC CGAGGTGGTC AAGTACCGGC 
     1441 AGTTATGCTT CAAGTCACAG TACAAGCGCT ACCTCAACTC CCAGCAGCAG TATTTCCATC 
     1501 GGCTGCTGAA GCAAATTCTT GCTTCCCGGA GTGATCTGCT GGAGATGGCC CGGCGGAGTG 
     1561 GCCCCGCCCT TCCCTTCCGG CAGAAACGCC CTTCACCATC CCGCACACCT GAGGAGCGGG 
     1621 AGTGGCGGAC CCAGCAGCGC TACTTGAAGG TCTTAAGGGA AGTGAAAGAG GAGTGTGGTG 
     1681 ACACAGCCCT GTCATCTGAT GAAGAGGCCA CGTTGGATTT ACAAGAACAA TTTTCTTTTG 
     1741 AAGATCTCAG CTCATGGCTT CCGAGCTCTC CAGCACGTTC TCCTAGTCCT GCGGTGCCCC 
     1801 TGCGGGTGGT GCCCACACTT TCAACCACGG ATATGAAAAC CGCAGATAAA GTAGAACTGG 
     1861 GGGACAGTGA CCTGAAGATA ATGTTAAAGA AGCACCACGA GAAGCGGAAA CATCAGCCAG 
     1921 ATCACCCGGA CCTTTTGACA GGGGACCTGA CTCTCAATGA CATCATGACT CGAGTAAATG 
     1981 CTGGCAGGAA GGGCTCTCTG GCAGCCTTAT ATGACTTGGC TGTCCTTAAA AAAAAGGTTA 
     2041 AGGAAAAAGA GGAAAAGAAG AAGAAGAAAA TAAAAACGAT CAAATCAGAG GCAGAGGACC 
     2101 TGGCCGAGCC GCTAAGCAGT ACTGAAGGGG TCGCACCTCT CTCACAGGCC CCCTCTCCGC 
     2161 TGGCAATTCC TGCTATCAAG GAAGAGCCCC TTGAAGACCT CAAGCCTTGC CTTGGAATCA 
     2221 ATGAAATATC TTCCAGCTTC TTCTCTCTTC TATTAGAGAT CTTGCTGCTG GAGAGTCAGG 
     2281 CTAGCCTTCC TATGCTAGAG GAGCGAGTTT TGGATTGGCA GTCATCGCCA GCCAGCTCCC 
     2341 TCAACAGCTG GTTCTCTGCG GCCCCCAACT GGGCTGAGTT GGTACTACCA GCCCTGCAGT 
     2401 ATCTTGCTGG AGAAAGTCGA GCTGTTCCTT CCAGTTTCTC TCCATTTGTT GAATTCAAAG 
     2461 AGAAAACCCA GCAGTGGAAG TTGCTTGGCC AATCCCAAGA TAATGAAAAG GAATTAGCTG 
     2521 CTCTCTTCCA GCTATGGCTA GAGACCAAAG ATCAGGCCTT CTGTAAGCAA GAAAATGAAG 
     2581 ACAGCTCAGA TGCCACAACA CCTGTCCCTC GGGTAAGAAC TGACTATGTG GTGCGTCCCA 
     2641 GCACGGGGGA GGAGAAACGG GTTTTTCAGG AGCAGGAGCG TTACAGGTAT AGCCAACCCC 
     2701 ATAAGGCGTT CACCTTTCGC ATGCACGGCT TTGAGTCTGT GGTGGGGCCA GTGAAGGGCG 
     2761 TGTTTGACAA GGAGACCTCG CTCAACAAGG CTCGGGAGCA CTCCCTGCTG CGCTCCGACC 
     2821 GGCCTGCCTA CGTCACCATT CTGTCTCTTG TTCGGGACGC TGCGGCTCGA CTGCCTAATG 
     2881 GAGAAGGCAC ACGGGCAGAG ATCTGTGAAC TGCTTAAGGA CTCCCAGTTT CTTGCACCAG 
     2941 ATGTCACCAG CACTCAGGTA AATACAGTAG TGAGTGGTGC ACTGGATCGG CTACATTACG 
     3001 AAAAAGATCC CTGTGTGAAA TACGACATTG GACGAAAGCT GTGGATCTAC CTGCATCGTG 
     3061 ACCGGAGTGA AGAAGAGTTT GAGCGGATTC ACCAAGCACA AGCAGCTGCA GCTAAAGCCA 
     3121 GAAAAGCTCT TCAGCAAAAA CCCAAGCCCC CATCCAAGGT GAAGTCCAGT AGCAAGGAGA 
     3181 GCTCCATAAA GGTCCTTAGC AGTGGCCCTT CTGAGCAGAG CCAGATGAGC CTCAGTGACT 
     3241 CCAGTATGCC ACCCACCCCA GTCACACCTG TAACCCCCAC CACACCAGCA TTGCCCGCCA 
     3301 TTCCCATCTC CCCTCCACCT GTATCGGCAG TGAACAAAAG CGGCCCTTCC ACAGTCTCAG 
     3361 AACCAGCTAA GTCTAGCTCG GGTGTTCTTC TGGTGTCTTC ACCAACAATG CCACATCTGG 
     3421 GAACAATGCT TTCCCCAGCT TCCAGCCAGA CTGCACCCAG TTCTCAGGCT GCCGCCCGGG 
     3481 TCGTGAGCCA CTCTGGCTCT GCTGGACTGT CTCAGGTGCG AGTGGTGGCC CAGCCTAGCC 
     3541 TTCCTGCTGT TCCCCAGCAG TCGGGAGGGC CGGCACAGAC ATTGCCACAG ATGCCAGCAG 
     3601 GACCGCAGAT CCGGGTTCCA GCCACTGCCA CACAGACCAA AGTAGTGCCC CAGACAGTAA 
     3661 TGGCCACTGT GCCCGTCAAA GCGCAGACTA CGGCAGCCAC TGTGCAGCGG CCTGGACCCG 
     3721 GGCAGACAGG GCTCACGGTG ACAAGTCTCC CTGCCACAGC CAGCCCTGTG AGTAAGCCAG 
     3781 CCACGAGTTC TCCTGGGACC TCTGCTCCCA GTGCCTCCAC GGCTGCCGTC ATTCAAAATG 
     3841 TCACAGGACA GAACATCATC AAGCAGGTGG CAATCACTGG GCAGCTTGGT GTGAAGCCCC 
     3901 AAACAGGCAA CAGCATTCCA CTCACAGCCA CTAACTTCCG CATCCAGGGT AAGGATGTAT 
     3961 TGCGTCTGCC GCCCTCTTCC ATCACCACAG ATGCCAAGGG CCAGACGGTT CTGCGAATCA 
     4021 CTCCGGACAT GATGGCCACA TTGGCCAAGT CCCAGGTTAC CACAGTCAAA TTGACCCAGG 
     4081 ACCTCTTCGG GACAGGAGGC AACACTACAG GCAAAGGCAT CTCTGCCACC TTACACGTCA 
     4141 CTTCCAATCC AGTACATGCA GCTGATAGCC CTGCCAAGGC CAGTTCAGCC AGTGCCCCTT 
     4201 CATCCACTCC AACAGGTACC ACTGTGGTCA AAGTGACTCC TGACCTCAAG CCAACAGAAG 
     4261 CCTCAAGTTC GGCTTTTCGC TTGATGCCAG CTCTTGGCGT GAGTGTGGCT GACCAGAAGG 
     4321 GAAAAAGCAC AGTGGCCTCT TCAGAAGCAA AACCAGCTGC CACGATCCGC ATCGTGCAGG 
     4381 GACTGGGAGT GATGCCTCCC AAAGCAGGCC AGACCATCAC CGTTGCAACC CACGCCAAGC 
     4441 AAGGGGCCTC GGTGGCCAGT GGGTCTGGAA CTGTCCATAC TTCAGCGGTG TCCTTACCCA 
     4501 GTATGAATGC TGCTGTGTCC AAGACTGTAG CTGTGGCTTC TGGGGCTGCA AGCACCCCCA 
     4561 TCAGCATCAG CACAGGAGCC CCCACCGTGC GGCAGGTCCC TGTCAGCACC ACGGTTGTGT 
     4621 CCACGTCCCA GGCTGGGAAG TTGCCTACAC GGATCACAGT TCCCCTCTCT GTGATCAGCC 
     4681 AGCCAATGAA GGGCAAGAGC GTGGTCACAG CCCCCATCAT CAAAGGCAAC CTTGGAGCCA 
     4741 ACCTCAGTGG GTTGGGCCGC AACATCATCC TCACAACTAT GCCAGCAGGC ACTAAGCTCA 
     4801 TTGCTGGCAA TAAGCCTGTT AGTTTCCTCA CTGCTCAGCA GTTGCAGCAG CTTCAGCAGC 
     4861 AAGGTCAGGC CACACAGGTG CGCATCCAGA CTGTCCCTGC ATCCCATCTC CAACAGGGAA 
     4921 CAGCTTCTGG CTCCTCCAAA GCAGTCTCCA CTGTTGTTGT GACTACAGCT CCGTCTCCTA 
     4981 AACAGGCACC TGAGCAACAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5041 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5101 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5161 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5221 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5281 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5341 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5401 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5461 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5521 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5581 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5641 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5701 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5761 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5821 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5881 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5941 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6001 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6061 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6121 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6181 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6241 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6301 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6361 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6421 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6481 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6541 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6601 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6661 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6721 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6781 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6841 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6901 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6961 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7021 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7081 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7141 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7201 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7261 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7321 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7381 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7441 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7501 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7561 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7621 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7681 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7741 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7801 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7861 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7921 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7981 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8041 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8101 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8221 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8281 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8341 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8401 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8461 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8521 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8581 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8641 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8701 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8761 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8821 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//