LOCUS       RC215023L1                9306 bp    DNA     circular  SYN 
DEFINITION  ERN1 (Myc-DDK-tagged)-Human endoplasmic reticulum to nucleus signaling 1 (ERN1)
ACCESSION   NM_001433
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7597..7777 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8505..9164 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7936..8504 
                     /label="ColE1"  
     misc_feature    6472..6492 
                     /label="LR50 primer"  
     misc_feature    6269..6340 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6481..7072 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6251..6257 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6251 
                     /label="ERN1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCGGCCCGG CGGCTGCTGC TGCTGCTGAC 
     3361 GCTGCTGCTG CCCGGCCTCG GGATTTTTGG AAGTACCAGC ACAGTGACGC TTCCTGAAAC 
     3421 CTTGTTGTTT GTGTCAACGC TGGATGGAAG TTTGCATGCT GTCAGCAAGA GGACAGGCTC 
     3481 AATCAAATGG ACTTTAAAAG AAGATCCAGT CCTGCAGGTC CCAACACATG TGGAAGAGCC 
     3541 TGCCTTTCTC CCAGATCCTA ATGATGGCAG CCTGTATACG CTTGGAAGCA AGAATAATGA 
     3601 AGGCCTGACG AAACTTCCTT TTACCATCCC AGAATTGGTG CAGGCATCCC CATGCCGAAG 
     3661 TTCAGATGGA ATCCTCTACA TGGGTAAAAA GCAGGACATC TGGTATGTTA TTGACCTCCT 
     3721 GACCGGAGAG AAGCAGCAGA CTTTGTCATC GGCCTTTGCA GATAGTCTCT GCCCATCAAC 
     3781 CTCTCTTCTG TATCTTGGGC GAACAGAATA CACCATCACC ATGTACGACA CCAAAACCCG 
     3841 AGAGCTCCGG TGGAATGCCA CCTACTTTGA CTATGCGGCC TCACTGCCTG AGGACGACGT 
     3901 GGACTACAAG ATGTCCCACT TTGTGTCCAA TGGTGATGGG CTGGTGGTGA CTGTGGACAG 
     3961 TGAATCTGGG GACGTCCTAT GGATCCAAAA CTACGCCTCC CCTGTGGTGG CCTTTTATGT 
     4021 CTGGCAGCGG GAGGGTCTGA GGAAGGTGAT GCACATCAAT GTCGCTGTGG AGACCCTGCG 
     4081 CTATCTGACC TTCATGTCTG GGGAGGTGGG GCGCATCACA AAGTGGAAGT ACCCGTTCCC 
     4141 CAAGGAGACA GAGGCCAAGA GCAAGCTGAC GCCCACTCTG TATGTTGGGA AATACTCTAC 
     4201 CAGCCTCTAT GCCTCTCCCT CAATGGTACA CGAGGGGGTT GCTGTCGTGC CCCGCGGCAG 
     4261 CACACTTCCT TTGCTGGAAG GGCCCCAGAC TGATGGCGTC ACCATCGGGG ACAAGGGGGA 
     4321 GTGTGTGATC ACGCCCAGCA CGGACGTCAA GTTTGATCCC GGACTCAAAA GCAAGAACAA 
     4381 GCTCAACTAC TTGAGGAATT ACTGGCTTCT GATAGGACAC CATGAAACCC CACTGTCTGC 
     4441 GTCTACCAAG ATGCTGGAGA GATTTCCCAA CAATCTACCC AAACATCGGG AAAATGTGAT 
     4501 TCCTGCTGAT TCAGAGAAAA AGAGCTTTGA GGAAGTTATC AACCTGGTTG ACCAGACTTC 
     4561 AGAAAACGCA CCTACCACCG TGTCTCGGGA TGTGGAGGAG AAGCCCGCCC ATGCCCCTGC 
     4621 CCGGCCCGAG GCCCCCGTGG ACTCCATGCT TAAGGACATG GCTACCATCA TCCTGAGCAC 
     4681 CTTCCTGCTG ATTGGCTGGG TGGCCTTCAT CATCACCTAT CCCCTGAGCA TGCATCAGCA 
     4741 GCAGCAGCTC CAGCACCAGC AGTTCCAGAA GGAACTGGAG AAGATCCAGC TCCTGCAGCA 
     4801 GCAGCAGCAG CAGCTGCCCT TCCACCCACC TGGAGACACG GCTCAGGACG GCGAGCTCCT 
     4861 GGACACGTCT GGCCCGTACT CAGAGAGCTC GGGCACCAGC AGCCCCAGCA CGTCCCCCAG 
     4921 GGCCTCCAAC CACTCGCTCT GCTCCGGCAG CTCTGCCTCC AAGGCTGGCA GCAGCCCCTC 
     4981 CCTGGAACAA GACGATGGAG ATGAGGAAAC CAGCGTGGTG ATAGTTGGGA AAATTTCCTT 
     5041 CTGTCCCAAG GATGTCCTGG GCCATGGAGC TGAGGGCACA ATTGTGTACC GGGGCATGTT 
     5101 TGACAACCGC GACGTGGCCG TGAAGAGGAT CCTCCCCGAG TGTTTTAGCT TCGCAGACCG 
     5161 TGAGGTCCAG CTGTTGCGAG AATCGGATGA GCACCCGAAC GTGATCCGCT ACTTCTGCAC 
     5221 GGAGAAGGAC CGGCAATTCC AGTACATTGC CATCGAGCTG TGTGCAGCCA CCCTGCAAGA 
     5281 GTATGTGGAG CAGAAGGACT TTGCGCATCT CGGCCTGGAG CCCATCACCT TGCTGCAGCA 
     5341 GACCACCTCG GGCCTGGCCC ACCTCCACTC CCTCAACATC GTTCACAGAG ACCTAAAGCC 
     5401 ACACAACATC CTCATATCCA TGCCCAATGC ACACGGCAAG ATCAAGGCCA TGATCTCCGA 
     5461 CTTTGGCCTC TGCAAGAAGC TGGCAGTGGG CAGACACAGT TTCAGCCGCC GATCTGGGGT 
     5521 GCCTGGCACA GAAGGCTGGA TCGCTCCAGA GATGCTGAGC GAAGACTGTA AGGAGAACCC 
     5581 TACCTACACG GTGGACATCT TTTCTGCAGG CTGCGTCTTT TACTACGTAA TCTCTGAGGG 
     5641 CAGCCACCCT TTTGGCAAGT CCCTGCAGCG GCAGGCCAAC ATCCTCCTGG GTGCCTGCAG 
     5701 CCTTGACTGC TTGCACCCAG AGAAGCACGA AGACGTCATT GCACGTGAAT TGATAGAGAA 
     5761 GATGATTGCG ATGGATCCTC AGAAACGCCC CTCAGCGAAG CACGTGCTCA AACACCCGTT 
     5821 CTTCTGGAGC CTAGAGAAGC AGCTCCAGTT CTTCCAGGAC GTGAGCGACA GAATAGAAAA 
     5881 GGAATCCCTG GATGGCCCGA TCGTGAAGCA GTTAGAGAGA GGCGGGAGAG CCGTGGTGAA 
     5941 GATGGACTGG CGGGAGAACA TCACTGTCCC CCTCCAGACA GACCTGCGTA AATTCAGGAC 
     6001 CTATAAAGGT GGTTCTGTCA GAGATCTCCT CCGAGCCATG AGAAATAAGA AGCACCACTA 
     6061 CCGGGAGCTG CCTGCAGAGG TGCGGGAGAC GCTGGGGTCC CTCCCCGACG ACTTCGTGTG 
     6121 CTACTTCACA TCTCGCTTCC CCCACCTCCT CGCACACACC TACCGGGCCA TGGAGCTGTG 
     6181 CAGCCACGAG AGACTCTTCC AGCCCTACTA CTTCCACGAG CCCCCAGAGC CCCAGCCCCC 
     6241 AGTGACTCCA GACGCCCTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     6301 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     6361 CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA 
     6421 TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG 
     6481 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 
     6541 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 
     6601 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 
     6661 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 
     6721 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 
     6781 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 
     6841 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 
     6901 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 
     6961 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 
     7021 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 
     7081 CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 
     7141 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 
     7201 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 
     7261 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 
     7321 TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC 
     7381 ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT 
     7441 TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT 
     7501 GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG 
     7561 CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT 
     7621 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 
     7681 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 
     7741 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA 
     7801 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     7861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     7921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     7981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     8041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     8101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     8161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     8221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     8281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     8341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     8401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     8461 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC 
     8521 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 
     8581 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 
     8641 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 
     8701 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 
     8761 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 
     8821 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 
     8881 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 
     8941 TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 
     9001 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 
     9061 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 
     9121 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC 
     9181 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     9241 GAATGTATTT AGAAAAATAA ACAAATAGGG GTCCCGCGCA CATTTCCCCG AAAAGTGCCA 
     9301 CCTGAC
//