LOCUS       RC215004                8247 bp    DNA     circular  SYN 
DEFINITION  PPP1R3A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A)
ACCESSION   NM_002711
VERSION     NM_002711.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="PPP1R3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCTTCT GAAGTACCTA GTCAGATTAG CAAAGATAAT TTTTTAGAAG 
     1081 TTCCTAATTT ATCTGACTCT CTTTGTGAAG ATGAAGAAGT TACTTTCCAA CCTGGTTTCT 
     1141 CCCCTCAACC AAGTAGACGA GGTTCTGATT CTTCTGAAGA CATATACCTG GATACCCCAT 
     1201 CTTCAGGTAC TAGAAGAGTT TCATTTGCTG ATTCCTTTGG ATTCAATCTT GTGTCTGTTA 
     1261 AAGAATTTGA TTGCTGGGAA TTACCGAGTG CTTCAACCAC TTTTGACTTA GGGACGGACA 
     1321 TTTTCCACAC AGAAGAATAT GTTTTAGCCC CACTGTTTGA CTTGCCTTCT TCAAAAGAAG 
     1381 ATCTTATGCA ACAACTCCAA ATACAGAAAG CAATACTGGA GTCAACTGAG TCTCTTCTTG 
     1441 GGTCTACAAG TATCAAGGGT ATTATTCGAG TTTTGAATGT TTCTTTTGAG AAGTTAGTAT 
     1501 ATGTAAGAAT GTCTTTAGAT GACTGGCAGA CACATTATGA CATTTTAGCA GAATATGTTC 
     1561 CTAATTCATG TGATGGTGAA ACTGACCAGT TCTCCTTTAA GATTGTATTG GTTCCTCCTT 
     1621 ATCAAAAAGA TGGCAGTAAA GTTGAGTTTT GTATACGTTA TGAAACTTCT GTTGGTACAT 
     1681 TTTGGTCAAA TAATAATGGC ACAAATTATA CATTCATTTG TCAAAAGAAA GAACAAGAGC 
     1741 CGGAGCCTGT AAAACCATGG AAAGAAGTTC CTAACAGACA AATAAAAGGC TGCTTAAAGG 
     1801 TAAAATCAAG TAAAGAAGAA TCATCAGTAA CATCAGAAGA AAATAACTTT GAGAATCCAA 
     1861 AGAATACAGA TACCTATATC CCAACAATCA TTTGTTCTCA TGAGGACAAG GAAGATTTGG 
     1921 AAGCCAGTAA TCGAAATGTA AAAGATGTAA ACAGGGAACA TGATGAACAT AATGAAAAAG 
     1981 AATTAGAGTT GATGATAAAT CAACACTTAA TAAGAACCAG AAGTACTGCT TCCAGAGATG 
     2041 AAAGGAATAC ATTTTCAACA GATCCAGTCA ATTTTCCAAA TAAAGCAGAG GGGTTAGAGA 
     2101 AGAAGCAAAT CCATGGTGAA ATATGTACTG ACTTGTTCCA AAGGTCTCTG TCTCCAAGTT 
     2161 CATCAGCAGA AAGCTCCGTA AAGGGAGATT TTTACTGCAA TGAAAAATAT TCCTCAGGAG 
     2221 ATGACTGTAC ACATCAACCT TCAGAGGAAA CTACTTCAAA TATGGGAGAA ATCAAGCCAT 
     2281 CATTGGGAGA TACTAGTAGT GATGAACTAG TGCAATTACA TACTGGCAGC AAAGAAGTCC 
     2341 TGGATGATAA TGCTAATCCA GCCCATGGCA ATGGCACAAT GCAAATACCT TGCCCCTCTT 
     2401 CAGATCAACT AATGGCAGGA AACCTTAATA AAAAACATGA AGGAGGAGCT AAAAAAATTG 
     2461 AAGTAAAAGA TTTGGGATGT TTACGAAGAG ATTTCCATTC AGATACGTCG GCATGTCTCA 
     2521 AAGAATCAAC AGAAGAAGGA TCTTCTAAGG AAGATTATTA TGGCAATGGT AAGGATGATG 
     2581 AAGAACAAAG AATATATTTA GGTGTTAATG AAAAACAAAG AAAAAATTTC CAAACAATCT 
     2641 TACATGACCA AGAAAGGAAG ATGGGTAACC CTAAAATAAG TGTGGCAGGG ATTGGAGCTA 
     2701 GTAACAGAGA CCTGGCTACT CTGCTGAGCG AACATACCGC AATCCCCACC CGGGCAATCA 
     2761 CAGCAGATGT GTCTCATTCA CCAAGGACAA ATTTAAGTTG GGAAGAAGCT GTGTTAACCC 
     2821 CAGAGCATCA TCATTTGACT AGTGAAGGCA GCGCTTTAGG AGGGATAACT GGTCAAGTTT 
     2881 GTTCATCAAG AACTGGAAAT GTTTTGAGGA ATGATTATCT TTTCCAAGTT GAAGAAAAAT 
     2941 CAGGTGGGAT TAATTCTGAA GATCAGGATA ATAGCCCACA GCATAAACAA AGTTGGAATG 
     3001 TTCTGGAAAG TCAGGGAAAA TCAAGAGAGA ATAAGACAAA CATAACAGAG CATATCAAAG 
     3061 GACAAACAGA TTGTGAAGAC GTGTGGGGAA AAAGAGATAA TACGAGGAGT TTGAAAGCTA 
     3121 CTACAGAAGA ATTGTTTACC TGCCAAGAAA CAGTGTGCTG TGAACTGTCT TCTCTAGCTG 
     3181 ATCATGGCAT TACTGAGAAA GCAGAAGCTG GTACAGCCTA TATAATTAAG ACAACATCAG 
     3241 AAAGTACTCC AGAAAGCATG TCTGCTAGAG AAAAAGCAAT AATTGCTAAG CTACCTCAAG 
     3301 AGACAGCACG AAGTGACAGG CCCATCGAGG TAAAGGAAAC AGCGTTTGAT CCACATGAAG 
     3361 GGAGAAATGA TGATTCACAT TATACCCTTT GTCAACGAGA TACAGTAGGT GTAATCTATG 
     3421 ACAATGATTT TGAAAAGGAA TCACGTTTAG GTATTTGTAA TGTACGTGTA GATGAAATGG 
     3481 AGAAGGAAGA AACCATGTCT ATGTACAATC CTAGGAAGAC ACATGACAGG GAGAAATGTG 
     3541 GCACTGGAAA TATAACATCT GTGGAAGAAT CCTCATGGGT CATTACAGAA TATCAAAAAG 
     3601 CAACTTCAAA ACTGGATTTA CAGTTGGGAA TGTTACCAAC AGACAAAACT GTATTTTCAG 
     3661 AAAACAGAGA TCATAGGCAG GTTCAAGAAT TATCAAAGAA AACAGACTCG GATGCCATTG 
     3721 TGCATTCTGC TTTTAACTCA GACACTAATA GAGCTCCTCA GAATAGCTCT CCTTTTTCCA 
     3781 AACATCATAC TGAAATTTCA GTGTCAACTA ATGAGCAGGC AATTGCTGTA GAGAATGCAG 
     3841 TTACTACCAT GGCTAGCCAA CCTATTTCTA CGAAATCAGA AAATATTTGT AATTCAACAA 
     3901 GAGAAATCCA GGGTATTGAG AAGCACCCTT ATCCTGAGTC TAAACCTGAA GAAGTTTCCA 
     3961 GAAGTTCAGG AATAGTGACA TCAGGTAGTA GAAAAGAAAG ATGCATAGGC CAGATTTTCC 
     4021 AAACAGAAGA GTATAGTGTG GAAAAATCTC TAGGGCCAAT GATTTTAATC AACAAACCTC 
     4081 TTGAGAATAT GGAAGAAGCA AGGCATGAAA ATGAAGGATT AGTAAGCTCT GGGCAATCAC 
     4141 TATACACTTC AGGTGAAAAG GAATCTGACA GCTCTGCTTC TACTAGTCTT CCTGTTGAGG 
     4201 AAAGTCAAGC TCAAGGCAAC GAATCTTTGT TTTCAAAATA TACCAACTCT AAAATACCTT 
     4261 ATTTCCTTTT GTTTCTGATA TTTCTTATAA CTGTCTACCA TTATGACTTA ATGATTGGCT 
     4321 TGACATTCTA CGTTTTGTCA TTGTCCTGGC TATCCTGGGA AGAGGGTAGA CAAAAAGAGT 
     4381 CTGTCAAAAA GAAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//