LOCUS       RC214961                9426 bp    DNA     circular  SYN 
DEFINITION  AGL (Myc-DDK-tagged)-Human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 5
ACCESSION   NM_000645
VERSION     NM_000645.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5583..5674 
                     /label="Myc-DDK"  
     misc_feature    5704..6289 
                     /label="PolyA signal"  
     misc_feature    5761..5780 
                     /label="Restriction Enzyme"  
     misc_feature    6565..7237 
                     /label="ColE1"  
     misc_feature    7667..8461 
                     /label="Kan/Neo"  
     misc_feature    8496..8798 
                     /label="SV40 ori"  
     misc_feature    8884..9361 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5565..5571 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5565 
                     /label="AGL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTATTA ACATGTGCTT TTTATTTAGG GTATGAGCTA CAGTTCCGAT 
     1081 TAGGCCCAAC TTTACAGGGA AAAGCAGTTA CCGTGTATAC AAATTACCCA TTTCCTGGAG 
     1141 AAACATTTAA TAGAGAAAAA TTCCGTTCTC TGGATTGGGA AAATCCAACA GAAAGAGAAG 
     1201 ATGATTCTGA TAAATACTGT AAACTTAATC TGCAACAATC TGGTTCATTT CAGTATTATT 
     1261 TCCTTCAAGG AAATGAGAAA AGTGGTGGAG GTTACATAGT TGTGGACCCC ATTTTACGTG 
     1321 TTGGTGCTGA TAATCATGTG CTACCCTTGG ACTGTGTTAC TCTTCAGACA TTTTTAGCTA 
     1381 AGTGTTTGGG ACCTTTTGAT GAATGGGAAA GCAGACTTAG GGTTGCAAAA GAATCAGGCT 
     1441 ACAACATGAT TCATTTTACC CCATTGCAGA CTCTTGGACT ATCTAGGTCA TGCTACTCCC 
     1501 TTGCCAATCA GTTAGAATTA AATCCTGACT TTTCAAGACC TAATAGAAAG TATACCTGGA 
     1561 ATGATGTTGG ACAGCTAGTG GAAAAATTAA AAAAGGAATG GAATGTTATT TGTATTACTG 
     1621 ATGTTGTCTA CAATCATACT GCTGCTAATA GTAAATGGAT CCAGGAACAT CCAGAATGTG 
     1681 CCTATAATCT TGTGAATTCT CCACACTTAA AACCTGCCTG GGTCTTAGAC AGAGCACTTT 
     1741 GGCGTTTCTC CTGTGATGTT GCAGAAGGGA AATACAAAGA AAAGGGAATA CCTGCTTTGA 
     1801 TTGAAAATGA TCACCATATG AATTCCATCC GAAAAATAAT TTGGGAGGAT ATTTTTCCAA 
     1861 AGCTTAAACT CTGGGAATTT TTCCAAGTAG ATGTCAACAA AGCGGTTGAG CAATTTAGAA 
     1921 GACTTCTTAC ACAAGAAAAT AGGCGAGTAA CCAAGTCTGA TCCAAACCAA CACCTTACGA 
     1981 TTATTCAAGA TCCTGAATAC AGACGGTTTG GCTGTACTGT AGATATGAAC ATTGCACTAA 
     2041 CGACTTTCAT ACCACATGAC AAGGGGCCAG CAGCAATTGA AGAATGCTGT AATTGGTTTC 
     2101 ATAAAAGAAT GGAGGAATTA AATTCAGAGA AGCATCGACT CATTAACTAT CATCAGGAAC 
     2161 AGGCAGTTAA TTGCCTTTTG GGAAATGTGT TTTATGAACG ACTGGCTGGC CATGGTCCAA 
     2221 AACTAGGACC TGTCACTAGA AAGCATCCTT TAGTTACCAG GTATTTTACT TTCCCATTTG 
     2281 AAGAGATAGA CTTCTCCATG GAAGAATCTA TGATTCATCT GCCAAATAAA GCTTGTTTTC 
     2341 TGATGGCACA CAATGGATGG GTAATGGGAG ATGATCCTCT TCGAAACTTT GCTGAACCGG 
     2401 GTTCAGAAGT TTACCTAAGG AGAGAACTTA TTTGCTGGGG AGACAGTGTT AAATTACGCT 
     2461 ATGGGAATAA ACCAGAGGAC TGTCCTTATC TCTGGGCACA CATGAAAAAA TACACTGAAA 
     2521 TAACTGCAAC TTATTTCCAG GGAGTACGTC TTGATAACTG CCACTCAACA CCTCTTCACG 
     2581 TAGCTGAGTA CATGTTGGAT GCTGCTAGGA ATTTGCAACC CAATTTATAT GTAGTAGCTG 
     2641 AACTGTTCAC AGGAAGTGAA GATCTGGACA ATGTCTTTGT TACTAGACTG GGCATTAGTT 
     2701 CCTTAATAAG AGAGGCAATG AGTGCATATA ATAGTCATGA AGAGGGCAGA TTAGTTTACC 
     2761 GATATGGAGG AGAACCTGTT GGATCCTTTG TTCAGCCCTG TTTGAGGCCT TTAATGCCAG 
     2821 CTATTGCACA TGCCCTGTTT ATGGATATTA CGCATGATAA TGAGTGTCCT ATTGTGCATA 
     2881 GATCAGCGTA TGATGCTCTT CCAAGTACTA CAATTGTTTC TATGGCATGT TGTGCTAGTG 
     2941 GAAGTACAAG AGGCTATGAT GAATTAGTGC CTCATCAGAT TTCAGTGGTT TCTGAAGAAC 
     3001 GGTTTTACAC TAAGTGGAAT CCTGAAGCAT TGCCTTCAAA CACAGGTGAA GTTAATTTCC 
     3061 AAAGCGGCAT TATTGCAGCC AGGTGTGCTA TCAGTAAACT TCATCAGGAG CTTGGAGCCA 
     3121 AGGGTTTTAT TCAGGTGTAT GTGGATCAAG TTGATGAAGA CATAGTGGCA GTAACAAGAC 
     3181 ACTCACCTAG CATCCATCAG TCTGTTGTGG CTGTATCTAG AACTGCTTTC AGGAATCCCA 
     3241 AGACTTCATT TTACAGCAAG GAAGTGCCTC AAATGTGCAT CCCTGGCAAA ATTGAAGAAG 
     3301 TAGTTCTTGA AGCTAGAACT ATTGAGAGAA ACACGAAACC TTATAGGAAG GATGAGAATT 
     3361 CAATCAATGG AACACCAGAT ATCACAGTAG AAATTAGAGA ACATATTCAG CTTAATGAAA 
     3421 GTAAAATTGT TAAACAAGCT GGAGTTGCCA CAAAAGGGCC CAATGAATAT ATTCAAGAAA 
     3481 TAGAATTTGA AAACTTGTCT CCAGGAAGTG TTATTATATT CAGAGTTAGT CTTGATCCAC 
     3541 ATGCACAAGT CGCTGTTGGA ATTCTTCGAA ATCATCTGAC ACAATTCAGT CCTCACTTTA 
     3601 AATCTGGCAG CCTAGCTGTT GACAATGCAG ATCCTATATT AAAAATTCCT TTTGCTTCTC 
     3661 TTGCCTCCAG ATTAACTTTG GCTGAGCTAA ATCAGATCCT TTACCGATGT GAATCAGAAG 
     3721 AAAAGGAAGA TGGTGGAGGG TGCTATGACA TACCAAACTG GTCAGCCCTT AAATATGCAG 
     3781 GTCTTCAAGG TTTAATGTCT GTATTGGCAG AAATAAGACC AAAGAATGAC TTGGGGCATC 
     3841 CTTTTTGTAA TAATTTGAGA TCTGGAGATT GGATGATTGA CTATGTCAGT AACCGGCTTA 
     3901 TTTCACGATC AGGAACTATT GCTGAAGTTG GTAAATGGTT GCAGGCTATG TTCTTCTACC 
     3961 TGAAGCAGAT CCCACGTTAC CTTATCCCAT GTTACTTTGA TGCTATATTA ATTGGTGCAT 
     4021 ATACCACTCT TCTGGATACA GCATGGAAGC AGATGTCAAG CTTTGTTCAG AATGGTTCAA 
     4081 CCTTTGTGAA ACACCTTTCA TTGGGTTCAG TTCAACTGTG TGGAGTAGGA AAATTCCCTT 
     4141 CCCTGCCAAT TCTTTCACCT GCCCTAATGG ATGTACCTTA TAGGTTAAAT GAGATCACAA 
     4201 AAGAAAAGGA GCAATGTTGT GTTTCTCTAG CTGCAGGCTT ACCTCATTTT TCTTCTGGTA 
     4261 TTTTCCGCTG CTGGGGAAGG GATACTTTTA TTGCACTTAG AGGTATACTG CTGATTACTG 
     4321 GACGCTATGT AGAAGCCAGG AATATTATTT TAGCATTTGC GGGTACCCTG AGGCATGGTC 
     4381 TCATTCCTAA TCTACTGGGT GAAGGAATTT ATGCCAGATA CAATTGTCGG GATGCTGTGT 
     4441 GGTGGTGGCT GCAGTGTATC CAGGATTACT GTAAAATGGT TCCAAATGGT CTAGACATTC 
     4501 TCAAGTGCCC AGTTTCCAGA ATGTATCCTA CAGATGATTC TGCTCCTTTG CCTGCTGGCA 
     4561 CACTGGATCA GCCATTGTTT GAAGTCATAC AGGAAGCAAT GCAAAAACAC ATGCAGGGCA 
     4621 TACAGTTCCG AGAAAGGAAT GCTGGTCCCC AGATAGATCG AAACATGAAG GACGAAGGTT 
     4681 TTAATATAAC TGCAGGAGTT GATGAAGAAA CAGGATTTGT TTATGGAGGA AATCGTTTCA 
     4741 ATTGTGGCAC ATGGATGGAT AAAATGGGAG AAAGTGACAG AGCTAGAAAC AGAGGAATCC 
     4801 CAGCCACACC AAGAGATGGG TCTGCTGTGG AAATTGTGGG CCTGAGTAAA TCTGCTGTTC 
     4861 GCTGGTTGCT GGAATTATCC AAAAAAAATA TTTTCCCTTA TCATGAAGTC ACAGTAAAAA 
     4921 GACATGGAAA GGCTATAAAG GTCTCATATG ATGAGTGGAA CAGAAAAATA CAAGACAACT 
     4981 TTGAAAAGCT ATTTCATGTT TCCGAAGACC CTTCAGATTT AAATGAAAAG CATCCAAATC 
     5041 TGGTTCACAA ACGTGGCATA TACAAAGATA GTTATGGAGC TTCAAGTCCT TGGTGTGACT 
     5101 ATCAGCTCAG GCCTAATTTT ACCATAGCAA TGGTTGTGGC CCCTGAGCTC TTTACTACAG 
     5161 AAAAAGCATG GAAAGCTTTG GAGATTGCAG AAAAAAAATT GCTTGGTCCC CTTGGCATGA 
     5221 AAACTTTAGA TCCAGATGAT ATGGTTTACT GTGGAATTTA TGACAATGCA TTAGACAATG 
     5281 ACAACTACAA TCTTGCTAAA GGTTTCAATT ATCACCAAGG ACCTGAGTGG CTGTGGCCTA 
     5341 TTGGGTATTT TCTTCGTGCA AAATTATATT TTTCCAGATT GATGGGCCCG GAGACTACTG 
     5401 CAAAGACTAT AGTTTTGGTT AAAAATGTTC TTTCCCGACA TTATGTTCAT CTTGAGAGAT 
     5461 CCCCTTGGAA AGGACTTCCA GAACTGACCA ATGAGAATGC CCAGTACTGT CCTTTCAGCT 
     5521 GTGAAACACA AGCCTGGTCA ATTGCTACTA TTCTTGAGAC ACTTTATGAT TTAACGCGTA 
     5581 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5641 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5701 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5761 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5821 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5881 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5941 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     6001 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     6061 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     6121 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     6181 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6241 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6301 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6361 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6421 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6481 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6541 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6601 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6661 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6721 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6781 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6841 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6901 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6961 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     7021 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     7081 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     7141 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     7201 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7261 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7321 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7381 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7441 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7501 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7561 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7621 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7681 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7741 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7801 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7861 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7921 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7981 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     8041 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     8101 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     8161 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     8221 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8281 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8341 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8401 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8461 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8521 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8581 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8641 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8701 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8761 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8821 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8881 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8941 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     9001 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     9061 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     9121 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     9181 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9241 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9301 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9361 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9421 AATTTT
//