LOCUS       RC214930                8553 bp    DNA     circular  SYN 
DEFINITION  COPA (Myc-DDK-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 2
ACCESSION   NM_004371
VERSION     NM_004371.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4710..4801 
                     /label="Myc-DDK"  
     misc_feature    4831..5416 
                     /label="PolyA signal"  
     misc_feature    4888..4907 
                     /label="Restriction Enzyme"  
     misc_feature    5692..6364 
                     /label="ColE1"  
     misc_feature    6794..7588 
                     /label="Kan/Neo"  
     misc_feature    7623..7925 
                     /label="SV40 ori"  
     misc_feature    8011..8488 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4692..4698 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4692 
                     /label="COPA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTAACCAAA TTCGAGACCA AGAGCGCGCG GGTCAAAGGG CTCAGCTTTC 
     1081 ACCCCAAAAG ACCTTGGATC CTGACTAGTT TACATAATGG GGTCATCCAG TTATGGGACT 
     1141 ATCGGATGTG CACTCTCATT GACAAGTTTG ATGAACATGA TGGTCCAGTG CGAGGCATTG 
     1201 ACTTCCATAA GCAGCAGCCA CTGTTCGTCT CTGGAGGAGA TGACTATAAG ATTAAGGTTT 
     1261 GGAATTACAA GCTTCGGCGC TGTCTTTTCA CATTGCTTGG GCACTTAGAT TATATTCGCA 
     1321 CCACGTTTTT TCATCATGAA TATCCCTGGA TTCTGAGTGC CTCCGATGAT CAGACCATCC 
     1381 GAGTGTGGAA CTGGCAATCT AGAACCTGTG TTTGTGTGTT AACAGGGCAC AACCATTATG 
     1441 TGATGTGTGC TCAGTTCCAC CCCACAGAAG ACTTGGTAGT ATCAGCCAGC CTGGACCAGA 
     1501 CTGTGCGCGT TTGGGATATT TCTGGTCTGA GGAAAAAAAA CCTGTCCCCT GGTGCGGTGG 
     1561 AATCGGATGT GAGAGGAATA ACTGGGGTTG ATCTATTTGG AACTACAGAT GCAGTGGTGA 
     1621 AGCATGTACT AGAGGGTCAC GATCGTGGAG TAAACTGGGC TGCCTTCCAC CCCACTATGC 
     1681 CCCTTATTGT ATCTGGGGCA GATGATCGTC AAGTGAAGAT CTGGCGCATG AATGAATCAA 
     1741 AGGCATGGGA GGTTGATACC TGCCGGGGCC ATTACAACAA TGTATCTTGT GCCGTCTTCC 
     1801 ACCCTCGCCA AGAGTTGATC CTCAGCAATT CTGAGGACAA GAGTATTCGA GTCTGGGATA 
     1861 TGTCTAAGCG GACTGGGGTT CAGACTTTCC GCAGAGACCA TGATCGTTTC TGGGTCCTAG 
     1921 CTGCTCACCC TAACCTTAAC CTCTTTGCAG CAGGCCATGA TGGTGGTATG ATTGTGTTTA 
     1981 AGCTGGAACG GGAACGGCCA GCCTATGCTG TTCATGGCAA TATGCTACAC TATGTCAAGG 
     2041 ACCGATTCTT ACGACAGCTG GATTTCAACA GCTCCAAAGA TGTAGCTGTG ATGCAGTTGC 
     2101 GGAGTGGTTC CAAGTTTCCA GTATTCAATA TGTCATACAA TCCAGCAGAA AATGCAGTCC 
     2161 TGCTTTGTAC AAGAGCTAGC AATCTAGAGA ATAGTACCTA TGACCTGTAC ACCATCCCTA 
     2221 AAGATGCTGA CTCCCAGAAT CCTGATGCGC CTGAAGGGAA ACGATCCTCA GGCCTGACAG 
     2281 CCGTTTGGGT CGCTCGAAAT CGGTTTGCTG TCCTAGATCG GATGCATTCG CTTCTGATCA 
     2341 AGAATCTGAA GAATGAGATC ACCAAAAAGG TACAGGTGCC CAACTGTGAT GAGATCTTCT 
     2401 ATGCTGGCAC AGGCAATCTC CTGCTTCGAG ATGCGGACTC TATCACACTC TTTGACGTAC 
     2461 AGCAGAAGCG GACTCTGGCA TCTGTGAAGA TTTCTAAAGT GAAATACGTT ATCTGGTCAG 
     2521 CAGACATGTC ACATGTAGCA CTACTAGCCA AACACGCCAT TGTGATCTGT AACCGCAAAC 
     2581 TGGATGCTTT ATGTAACATT CATGAGAACA TTCGTGTCAA GAGTGGGGCC TGGGATGAGA 
     2641 GTGGGGTATT TATCTATACC ACAAGCAACC ACATCAAATA TGCTGTCACC ACTGGGGACC 
     2701 ACGGGATCAT TCGAACTCTG GATTTACCCA TCTATGTCAC ACGGGTGAAG GGCAACAATG 
     2761 TATACTGCCT AGACAGGGAG TGTCGTCCCC GGGTACTCAC CATTGATCCC ACTGAGTTCA 
     2821 AATTCAAGCT GGCCCTGATC AACAGAAAAT ATGATGAGGT ACTGCACATG GTGAGGAATG 
     2881 CCAAACTAGT TGGCCAGTCT ATTATTGCTT ATCTCCAGAA GAAGGGCTAT CCTGAAGTGG 
     2941 CACTGCATTT TGTCAAGGAT GAGAAAACTC GCTTTAGTCT GGCACTGGAG TGTGGAAACA 
     3001 TTGAGATTGC TCTGGAAGCA GCCAAAGCAC TGGATGACAA GAACTGCTGG GAAAAGCTGG 
     3061 GAGAAGTGGC CCTGCTGCAG GGGAACCACC AGATTGTGGA AATGTGCTAT CAGCGTACCA 
     3121 AAAACTTTGA CAAACTTTCC TTCCTGTATC TTATCACTGG CAACTTAGAA AAACTTCGCA 
     3181 AGATGATGAA GATTGCTGAG ATCAGAAAGG ACATGAGTGG CCACTATCAG AATGCCCTAT 
     3241 ACCTGGGTGA TGTGTCAGAG CGTGTGCGGA TCCTGAAGAA CTGTGGACAG AAGTCCCTGG 
     3301 CCTATCTCAC AGCTGCTACC CATGGCTTAG ATGAAGAAGC TGAGAGCCTA AAGGAGACAT 
     3361 TTGACCCAGA GAAGGAGACA ATCCCAGACA TTGACCCTAA TGCCAAGCTG CTCCAGCCAC 
     3421 CTGCACCTAT CATGCCATTG GATACCAATT GGCCTTTATT GACTGTATCC AAAGGATTTT 
     3481 TTGAAGGCAC CATTGCCAGC AAAGGGAAGG GAGGAGCACT GGCTGCTGAC ATTGACATTG 
     3541 ACACTGTTGG TACAGAGGGC TGGGGAGAGG ATGCAGAGCT GCAGTTGGAT GAAGATGGGT 
     3601 TTGTGGAGGC TACAGAAGGT TTGGGGGATG ATGCTCTTGG CAAGGGACAG GAAGAAGGAG 
     3661 GTGGCTGGGA TGTAGAAGAA GATCTGGAGC TCCCTCCTGA GCTGGATATA TCCCCTGGGG 
     3721 CAGCTGGTGG GGCTGAAGAT GGTTTCTTTG TGCCCCCAAC CAAGGGAACA AGTCCAACTC 
     3781 AGATCTGGTG TAATAACTCT CAGCTTCCAG TTGATCACAT CCTGGCAGGC TCTTTCGAAA 
     3841 CAGCCATGCG GCTCCTTCAT GACCAAGTAG GGGTAATCCA GTTTGGCCCC TACAAGCAAC 
     3901 TGTTCCTACA GACATACGCC CGAGGCCGCA CAACCTATCA GGCTCTGCCC TGCCTACCCT 
     3961 CCATGTATGG CTATCCTAAT CGCAACTGGA AGGATGCAGG GCTGAAGAAT GGTGTACCAG 
     4021 CTGTGGGCCT GAAGCTTAAT GACCTCATCC AACGGTTGCA GCTGTGCTAC CAGCTCACCA 
     4081 CAGTTGGCAA ATTTGAGGAG GCTGTGGAAA AATTCCGTTC CATCCTTCTC AGTGTGCCAC 
     4141 TTCTTGTTGT GGACAATAAA CAAGAGATTG CAGAGGCCCA GCAGCTCATC ACCATTTGCC 
     4201 GTGAGTACAT TGTGGGTTTG TCCGTGGAGA CAGAAAGGAA GAAGCTGCCC AAAGAGACTC 
     4261 TAGAACAGCA GAAGCGCATC TGTGAGATGG CAGCCTATTT CACCCACTCA AACCTGCAGC 
     4321 CTGTGCACAT GATCCTGGTG CTGCGTACAG CCCTCAATCT GTTCTTCAAG CTCAAGAACT 
     4381 TCAAGACAGC TGCCACCTTT GCTCGGCGCC TACTAGAACT CGGGCCCAAG CCTGAGGTGG 
     4441 CCCAACAGAC CCGAAAAATC CTGTCTGCCT GTGAGAAGAA TCCCACAGAT GCCTACCAGC 
     4501 TCAATTATGA CATGCACAAC CCCTTTGACA TTTGTGCTGC ATCATATCGG CCCATCTACC 
     4561 GTGGAAAGCC AGTAGAAAAG TGTCCACTCA GTGGGGCCTG CTATTCCCCT GAGTTCAAAG 
     4621 GTCAAATCTG CAGGGTCACC ACAGTGACAG AGATTGGCAA AGATGTGATT GGTTTAAGGA 
     4681 TCAGTCCTCT GCAGTTTCGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4741 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4801 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4861 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4921 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4981 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5041 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5101 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5161 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5221 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5281 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5341 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5401 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5461 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5521 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5581 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5641 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5701 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5761 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5821 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5881 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5941 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6001 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6061 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6121 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6181 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6241 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6301 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6361 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6421 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6481 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6541 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6601 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6661 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6721 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6781 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6841 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6901 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6961 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7021 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7081 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7141 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7201 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7261 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7321 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7381 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7441 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7501 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7561 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7621 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7681 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7741 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7801 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7981 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8041 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8101 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8161 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8221 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8281 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8341 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8401 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8461 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8521 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//