LOCUS       RC214900                8430 bp    DNA     circular  SYN 
DEFINITION  NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 2
ACCESSION   NM_005010
VERSION     NM_005010.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4587..4678 
                     /label="Myc-DDK"  
     misc_feature    4708..5293 
                     /label="PolyA signal"  
     misc_feature    4765..4784 
                     /label="Restriction Enzyme"  
     misc_feature    5569..6241 
                     /label="ColE1"  
     misc_feature    6671..7465 
                     /label="Kan/Neo"  
     misc_feature    7500..7802 
                     /label="SV40 ori"  
     misc_feature    7888..8365 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4569..4575 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4569 
                     /label="NRCAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCTTAAA ATAATGCCGA AAAAGAAGCG CTTATCTGCG GGCAGAGTGC 
     1081 CCCTGATTCT CTTCCTGTGC CAGATGATTA GTGCACTGGA AGTACCTCTT GATCTGGTAC 
     1141 AGCCTCCAAC CATCACCCAA CAGTCTCCAA AAGATTACAT TATTGACCCT CGGGAGAATA 
     1201 TTGTAATCCA GTGTGAAGCC AAAGGGAAAC CGCCCCCAAG CTTTTCCTGG ACCCGTAATG 
     1261 GGACTCATTT TGACATCGAT AAAGACCCTC TGGTCACCAT GAAGCCTGGC ACAGGAACGC 
     1321 TCATAATTAA CATCATGAGC GAAGGGAAAG CTGAGACCTA TGAAGGAGTC TATCAGTGTA 
     1381 CAGCAAGGAA CGAACGCGGA GCTGCAGTTT CTAATAACAT TGTTGTCCGC CCATCCAGAT 
     1441 CACCATTGTG GACCAAAGAA AAACTTGAAC CAATCACACT TCAAAGTGGT CAGTCTTTAG 
     1501 TACTTCCCTG CAGACCCCCA ATTGGATTAC CACCACCTAT AATATTTTGG ATGGATAATT 
     1561 CCTTTCAAAG ACTTCCACAA AGTGAGAGAG TTTCTCAAGG TTTGAATGGG GACCTTTATT 
     1621 TTTCCAATGT CCTCCCAGAG GACACCCGCG AAGACTATAT CTGTTATGCT AGATTTAATC 
     1681 ATACTCAAAC CATACAGCAG AAGCAACCTA TTTCTGTGAA GGTGATTTCA GTGGATGAAT 
     1741 TGAATGACAC TATAGCTGCT AATTTGAGTG ACACTGAGTT TTATGGTGCT AAATCAAGTA 
     1801 GAGAGAGGCC ACCAACATTT TTAACTCCAG AAGGCAATGC AAGTAACAAA GAGGAATTAA 
     1861 GAGGAAATGT GCTTTCACTG GAGTGCATTG CAGAAGGACT GCCTACCCCA ATTATTTACT 
     1921 GGGCAAAGGA AGATGGAATG CTACCCAAAA ACAGGACAGT TTATAAGAAC TTTGAGAAAA 
     1981 CCTTGCAGAT CATTCATGTT TCAGAAGCAG ACTCTGGAAA TTACCAATGT ATAGCAAAAA 
     2041 ACGCATTAGG AGCCATCCAC CATACCATTT CTGTTAGAGT TAAAGCGGCT CCATACTGGA 
     2101 TCACAGCCCC TCAAAATCTT GTGCTGTCCC CAGGAGAGGA TGGGACCTTG ATCTGCAGAG 
     2161 CTAATGGCAA CCCCAAACCC AGAATTAGCT GGTTAACAAA TGGAGTCCCA ATAGAAATTG 
     2221 CCCCTGATGA CCCCAGCAGA AAAATAGATG GCGATACCAT TATTTTTTCA AATGTTCAAG 
     2281 AAAGATCAAG TGCAGTCTAT CAGTGCAATG CCTCTAATGA ATATGGATAT TTACTGGCAA 
     2341 ACGCATTTGT AAATGTGCTG GCTGAGCCAC CACGAATCCT CACACCTGCA AACACACTCT 
     2401 ACCAGGTCAT TGCAAACAGG CCTGCTTTAC TAGACTGTGC CTTCTTTGGG TCTCCTCTCC 
     2461 CAACCATCGA GTGGTTTAAA GGAGCTAAAG GAAGTGCTCT TCATGAAGAT ATTTATGTTT 
     2521 TACATGAAAA TGGAACTTTG GAAATTCCTG TGGCCCAAAA GGACAGTACA GGAACTTATA 
     2581 CGTGTGTTGC AAGGAATAAA TTAGGGATGG CGAAGAATGA AGTTCACTTA GAAATCAAAG 
     2641 ATCCTACATG GATCGTTAAA CAGCCCGAAT ATGCAGTTGT GCAAAGAGGG AGCATGGTGT 
     2701 CCTTTGAATG CAAAGTGAAA CATGATCACA CCTTATCCCT CACTGTCCTG TGGCTGAAGG 
     2761 ACAACAGGGA ACTGCCCAGT GATGAAAGGT TCACTGTTGA CAAGGATCAT CTAGTGGTAG 
     2821 CTGATGTCAG TGACGATGAC AGCGGGACCT ACACGTGTGT GGCCAACACC ACTCTGGACA 
     2881 GCGTCTCCGC CAGCGCTGTG CTTAGCGTTG TTGATGTCCC AAATCCTCCC TTTGACTTAG 
     2941 AACTGACAGA TCAACTTGAC AAAAGTGTTC AGCTGTCATG GACCCCAGGC GATGACAACA 
     3001 ATAGCCCCAT TACAAAATTC ATCATCGAAT ATGAAGATGC AATGCACAAG CCAGGGCTGT 
     3061 GGCACCACCA AACTGAAGTT TCTGGAACAC AGACCACAGC CCAGCTGAAG CTGTCTCCTT 
     3121 ACGTGAACTA CTCCTTCCGC GTGATGGCAG TGAACAGCAT TGGGAAGAGC TTGCCCAGCG 
     3181 AGGCGTCTGA GCAGTATTTG ACGAAAGCCT CAGAACCAGA TAAAAACCCC ACAGCTGTGG 
     3241 AAGGACTGGG ATCAGAGCCT GATAATTTGG TGATTACGTG GAAGCCCTTG AATGGTTTCG 
     3301 AATCTAATGG GCCAGGCCTT CAGTACAAAG TTAGCTGGCG CCAGAAAGAT GGTGATGATG 
     3361 AATGGACATC TGTGGTTGTG GCAAATGTAT CCAAATATAT TGTCTCAGGC ACGCCAACCT 
     3421 TTGTTCCATA CCTGATCAAA GTTCAGGCCC TGAATGACAT GGGGTTTGCC CCCGAGCCAG 
     3481 CTGTAGTCAT GGGACATTCT GGAGAAGACC TCCCAATGGT GGCTCCTGGG AACGTGCGTG 
     3541 TGAATGTGGT GAACAGTACC TTAGCCGAGG TGCACTGGGA CCCAGTACCT CTGAAAAGCA 
     3601 TCCGAGGACA CCTACAAGGC TATCGGATTT ACTATTGGAA GACCCAGAGT TCATCTAAAA 
     3661 GAAACAGACG TCACATTGAG AAAAAGATCC TCACCTTCCA AGGCAGCAAG ACTCATGGCA 
     3721 TGTTGCCGGG GCTAGAGCCC TTTAGCCACT ACACACTGAA TGTCCGAGTG GTCAATGGGA 
     3781 AAGGGGAGGG CCCAGCCAGC CCTGACAGAG TCTTTAATAC TCCAGAAGGA GTCCCCAGTG 
     3841 CTCCCTCGTC TTTGAAGATT GTGAATCCAA CACTGGACTC TCTCACTTTG GAATGGGATC 
     3901 CACCGAGCCA CCCGAATGGC ATTTTGACAG AGTACACCTT AAAGTATCAG CCAATTAACA 
     3961 GCACACATGA ATTAGGCCCT CTGGTAGATT TGAAAATTCC TGCCAACAAG ACACGGTGGA 
     4021 CTTTAAAAAA TTTAAATTTC AGCACTCGAT ATAAGTTTTA TTTCTATGCA CAAACATCAG 
     4081 CAGGATCAGG AAGTCAAATT ACAGAGGAAG CAGTAACAAC TGTGGATGAA GCGATGGCAA 
     4141 GCCGGCAGGT GGATATTGCA ACTCAGGGCT GGTTCATTGG TCTGATGTGT GCTGTTGCTC 
     4201 TCCTTATCTT AATTTTGCTG ATTGTTTGCT TCATCAGAAG AAACAAGGGT GGTAAATATC 
     4261 CAGTTAAAGA AAAGGAAGAT GCCCATGCTG ACCCTGAAAT CCAGCCTATG AAGGAAGATG 
     4321 ATGGGACATT TGGAGAATAC AGTGATGCAG AAGACCACAA GCCTTTGAAA AAAGGAAGTC 
     4381 GAACTCCTTC AGACAGGACT GTGAAAAAAG AAGATAGTGA CGACAGCCTA GTTGACTATG 
     4441 GAGAAGGGGT TAATGGCCAG TTCAATGAGG ATGGCTCCTT TATTGGACAA TACAGTGGTA 
     4501 AGAAAGAGAA AGAGCCGGCT GAAGGAAACG AAAGCTCAGA GGCACCTTCT CCTGTCAACG 
     4561 CCATGAATTC CTTTGTTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4621 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4681 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4741 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4801 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4861 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4921 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4981 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5041 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5101 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5161 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5221 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5281 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5341 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5401 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5461 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5521 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5581 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5641 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5701 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5761 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5821 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5881 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5941 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6001 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6061 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6121 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6181 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6241 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6301 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6361 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6421 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6481 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6541 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6601 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6661 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6721 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6781 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6841 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6901 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6961 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7021 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7081 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7141 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7201 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7261 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7321 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7381 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7441 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7501 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7561 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7621 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7681 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7741 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7861 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7921 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7981 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8041 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8101 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8161 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8221 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8281 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8341 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8401 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//