LOCUS       RC214874                8844 bp    DNA     circular  SYN 
DEFINITION  TTBK1 (Myc-DDK-tagged)-Human tau tubulin kinase 1 (TTBK1)
ACCESSION   NM_032538
VERSION     NM_032538.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5001..5092 
                     /label="Myc-DDK"  
     misc_feature    5122..5707 
                     /label="PolyA signal"  
     misc_feature    5179..5198 
                     /label="Restriction Enzyme"  
     misc_feature    5983..6655 
                     /label="ColE1"  
     misc_feature    7085..7879 
                     /label="Kan/Neo"  
     misc_feature    7914..8216 
                     /label="SV40 ori"  
     misc_feature    8302..8779 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4983..4989 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4983 
                     /label="TTBK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTGCCTA GCGGCCGCCC TTAAGGACGA AACCAACATG AGTGGGGGAG 
     1081 GGGAGCAGGC CGACATCCTG CCGGCCAACT ACGTGGTCAA GGATCGCTGG AAGGTGCTGA 
     1141 AAAAGATCGG GGGCGGGGGC TTTGGTGAGA TCTACGAGGC CATGGACCTG CTGACCAGGG 
     1201 AGAATGTGGC CCTCAAGGTG GAGTCAGCCC AGCAGCCCAA GCAGGTCCTC AAGATGGAGG 
     1261 TGGCCGTGCT CAAGAAGTTG CAAGGGAAGG ACCATGTGTG CAGGTTCATT GGCTGTGGCA 
     1321 GGAACGAGAA GTTTAACTAT GTAGTGATGC AGCTCCAGGG CCGGAACCTG GCCGACCTGC 
     1381 GCCGTAGCCA GCCGCGAGGC ACCTTCACGC TGAGCACCAC ATTGCGGCTG GGCAAGCAGA 
     1441 TCTTGGAGTC CATCGAGGCC ATCCACTCTG TGGGCTTCCT GCACCGTGAC ATCAAGCCTT 
     1501 CAAACTTTGC CATGGGCAGG CTGCCCTCCA CCTACAGGAA GTGCTATATG CTGGACTTCG 
     1561 GGCTGGCCCG GCAGTACACC AACACCACGG GGGATGTGCG GCCCCCTCGG AATGTGGCCG 
     1621 GGTTTCGAGG AACGGTTCGC TATGCCTCAG TCAATGCCCA CAAGAACCGG GAGATGGGCC 
     1681 GCCACGACGA CCTGTGGTCC CTCTTCTACA TGCTGGTGGA GTTTGCAGTG GGCCAGCTGC 
     1741 CCTGGAGGAA GATCAAGGAC AAGGAACAGG TAGGGATGAT CAAGGAGAAG TATGAGCACC 
     1801 GGATGCTGCT GAAGCACATG CCGTCAGAGT TCCACCTCTT CCTGGACCAC ATTGCCAGCC 
     1861 TCGACTACTT CACCAAGCCC GACTACCAGT TGATCATGTC AGTGTTTGAG AACAGCATGA 
     1921 AGGAGAGGGG CATTGCCGAG AATGAGGCCT TTGACTGGGA GAAGGCAGGC ACCGATGCCC 
     1981 TCCTGTCCAC GAGCACCTCT ACCCCGCCCC AGCAGAACAC CCGGCAGACG GCAGCCATGT 
     2041 TTGGGGTGGT CAATGTGACG CCAGTGCCTG GGGACCTGCT CCGGGAGAAC ACCGAGGATG 
     2101 TGCTACAGGG AGAGCACCTG AGTGACCAGG AGAATGCACC CCCAATTCTG CCCGGGAGGC 
     2161 CCTCTGAGGG GCTGGGCCCC AGTCCCCACC TTGTCCCCCA CCCCGGGGGT CCTGAGGCTG 
     2221 AAGTCTGGGA GGAGACAGAT GTCAACCGGA ACAAACTCCG GATCAACATC GGCAAAAGCC 
     2281 CCTGTGTGGA GGAGGAACAG AGCCGAGGCA TGGGGGTCCC CAGCTCCCCA GTGCGTGCCC 
     2341 CCCCAGACTC CCCCACAACC CCAGTCCGTT CTCTGCGCTA CCGGAGGGTG AACAGCCCTG 
     2401 AGTCAGAAAG GCTGTCCACG GCGGACGGGC GAGTGGAGCT ACCTGAGAGG AGGTCACGGA 
     2461 TGGATCTGCC TGGCTCGCCC TCGCGCCAGG CCTGCTCCTC TCAGCCAGCC CAGATGCTGT 
     2521 CAGTGGACAC AGGCCACGCT GACCGACAGG CCAGTGGCCG CATGGACGTG TCAGCCTCTG 
     2581 TGGAGCAGGA GGCCCTGAGC AACGCCTTCC GCTCGGTGCC GCTGGCTGAG GAGGAGGATT 
     2641 TCGACAGCAA AGAGTGGGTC ATCATCGACA AGGAGACGGA GCTCAAGGAC TTCCCTCCAG 
     2701 GGGCTGAGCC CAGCACATCG GGCACCACGG ATGAGGAGCC CGAGGAGCTG CGGCCACTGC 
     2761 CCGAGGAGGG CGAAGAGCGG CGGCGGCTGG GGGCAGAGCC CACCGTCCGG CCCCGGGGAC 
     2821 GCAGCATGCA GGCGCTGGCG GAGGAGGACC TGCAGCATTT GCCGCCCCAG CCCCTGCCAC 
     2881 CCCAGCTGAG CCAGGGCGAT GGCCGTTCCG AGACGTCACA GCCCCCCACG CCTGGCAGCC 
     2941 CTTCCCACTC ACCCCTGCAC TCGGGACCCC GCCCTCGACG GAGAGAGTCG GACCCCACAG 
     3001 GCCCACAGAG ACAGGTGTTC TCCGTGGCGC CCCCATTTGA GGTGAATGGC CTCCCACGAG 
     3061 CTGTGCCTCT GAGTCTGCCC TACCAGGACT TCAAAAGAGA CCTCTCCGAT TACCGAGAAC 
     3121 GGGCGCGGTT GCTCAACAGG GTCCGGAGGG TGGGCTTCTC GCACATGCTG CTCACCACCC 
     3181 CCCAGGTCCC ACTGGCTCCT GTTCAGCCTC AGGCTAATGG GAAGGAGGAA GAGGAGGAGG 
     3241 AGGAGGAAGA TGAGGAAGAG GAAGAAGAGG ATGAGGAAGA AGAAGAGGAG GAAGAGGAAG 
     3301 AGGAGGAGGA AGAAGAGGAG GAGGAGGAAG AGGAGGAGGA GGCTGCAGCG GCAGTTGCCT 
     3361 TGGGGGAGGT GCTGGGGCCT CGTAGTGGCT CCAGCAGTGA GGGGAGTGAG AGGAGCACTG 
     3421 ACCGGAGCCA GGAGGGTGCC CCGTCCACGC TGCTGGCAGA CGATCAGAAG GAGTCCAGGG 
     3481 GCCGGGCCTC CATGGCCGAT GGGGACCTGG AGCCTGAGGA GGGCTCCAAA ACGCTGGTGC 
     3541 TTGTCTCTCC TGGCGACATG AAGAAGTCGC CCGTCACTGC CGAACTGGCC CCCGACCCCG 
     3601 ACCTGGGCAC CCTGGCTGCC CTCACTCCTC AGCATGAGCG GCCCCAGCCC ACGGGCAGCC 
     3661 AGCTGGACGT ATCTGAGCCA GGCACCCTGT CCTCTGTCCT CAAGTCTGAG CCCAAGCCCC 
     3721 CGGGGCCTGG GGCAGGGCTG GGGGCCGGGA CAGTGACCAC AGGGGTCGGG GGCGTGGCAG 
     3781 TCACCTCCTC ACCCTTCACC AAAGTTGAGA GGACCTTTGT GCACATTGCG GAGAAAACCC 
     3841 ACCTCAACGT CATGTCTTCC GGTGGACAAG CCTTGCGGTC TGAGGAGTTC AGCGCTGGGG 
     3901 GCGAGCTGGG TCTGGAGCTG GCCTCTGATG GGGGCGCTGT GGAGGAGGGG GCCCGAGCGC 
     3961 CCCTGGAGAA CGGCCTCGCC CTGTCAGGGC TGAATGGGGC TGAGATAGAG GGCTCTGCCC 
     4021 TGTCTGGGGC CCCCCGGGAA ACCCCCTCAG AGATGGCCAC AAACTCACTG CCCAATGGCC 
     4081 CGGCCCTTGC AGACGGGCCA GCCCCGGTGT CCCCGCTGGA GCCAAGCCCT GAGAAAGTGG 
     4141 CCACCATCTC CCCCAGACGC CATGCTATGC CAGGCTCTCG CCCCAGGAGC CGTATCCCTG 
     4201 TCCTGCTCTC TGAGGAGGAC ACGGGCTCGG AGCCCTCAGG CTCACTGTCG GCCAAAGAGC 
     4261 GGTGGAGCAA GCGGGCTCGG CCGCAGCAGG ACCTGGCGCG GCTGGTGATG GAGAAGAGGC 
     4321 AGGGCCGCCT GCTGTTGCGG CTGGCCTCAG GGGCCTCGTC CTCCTCCAGT GAGGAGCAGC 
     4381 GCCGTGCCTC TGAGACCCTC TCAGGCACGG GCTCTGAGGA GGACACGCCC GCCTCTGAGC 
     4441 CGGCAGCGGC CTTGCCCAGG AAGAGCGGGA GGGCAGCCGC CACCAGGAGC CGGATTCCCC 
     4501 GCCCCATTGG CCTCCGCATG CCCATGCCTG TTGCAGCCCA GCAGCCCGCC AGCAGATCCC 
     4561 ATGGCGCGGC CCCAGCATTG GACACAGCCA TCACCAGCAG GCTCCAGCTG CAGACGCCCC 
     4621 CAGGGTCGGC CACTGCTGCT GACCTCCGCC CCAAACAACC TCCTGGCCGC GGCCTGGGCC 
     4681 CAGGGCGAGC CCAAGCCGGA GCCAGGCCCC CAGCGCCGCG CAGCCCGCGC CTCCCCGCGT 
     4741 CCACATCCGC CGCGCGCAAT GCCAGCGCGT CCCCCCGGAG CCAGTCCCTG TCCCGCAGAG 
     4801 AGAGCCCCTC CCCCTCGCAC CAGGCCCGGC CCGGGGTCCC CCCGCCCCGG GGCGTCCCGC 
     4861 CGGCCCGGGC CCAGCCTGAT GGCACCCCCT CCCCCGGGGG CTCCAAGAAA GGACCCAGAG 
     4921 GGAAACTCCA GGCTCAGCGC GCAACAACCA AAGGCCGGGC AGGAGGCGCG GAGGGCCGGG 
     4981 CTGGGGCCAG AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5041 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5101 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5161 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5221 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5281 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5341 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5401 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5461 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5521 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5581 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5641 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5701 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5761 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5821 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5881 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5941 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6001 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6061 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6121 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6181 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6241 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6301 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6361 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6421 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6481 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6541 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6601 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6661 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6721 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6781 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6841 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6901 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6961 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7021 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7081 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7141 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7201 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7261 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7321 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7381 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7441 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7501 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7561 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7621 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7681 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7741 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7801 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7861 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7921 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7981 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8041 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8281 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8341 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8401 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8461 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8521 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8581 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8641 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8701 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8761 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8821 TAACAAAAAT TTAACGCGAA TTTT
//