LOCUS       RC214854                7731 bp    DNA     circular  SYN 
DEFINITION  PCDHA4 (Myc-DDK-tagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 1
ACCESSION   NM_018907
VERSION     NM_018907.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3886..3977 
                     /label="Myc-DDK"  
     misc_feature    4007..4592 
                     /label="PolyA signal"  
     misc_feature    4064..4083 
                     /label="Restriction Enzyme"  
     misc_feature    4868..5540 
                     /label="ColE1"  
     misc_feature    5970..6764 
                     /label="Kan/Neo"  
     misc_feature    6799..7101 
                     /label="SV40 ori"  
     misc_feature    7187..7664 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3861..3868 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3861 
                     /label="PCDHA4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTTTTCC TGGGGAAGCG GCCAGGAATC CCGGCGTCTG CTGCTCTTAC 
     1081 TTCTTCTCCT CGCAGCCTGG GAGGCAGGGA ACGGTCAGCT CCACTACTCG GTCTCCGAGG 
     1141 AGGCCAAACA CGGCACCTTC GTGGGCCGCA TCGCGCAGGA CCTGGGACTG GAGCTGGCGG 
     1201 AGCTGGTGCC GCGCCTGTTC CGGGTGGCGT CCAAGGGCCG CGGAGGCCTT CTGGAGGTAA 
     1261 ATCTGCAGAA TGGCATTTTG TTTGTGAATT CTCGGATCGA CCGGGAGGAG CTGTGCCGGC 
     1321 GGAGCGCGGA GTGCAGCATC CACCTGGAGG TGATCGTAGA CAGGCCGCTG CAGGTTTTCC 
     1381 ATGTGGACGT GGAGGTGAGG GACATTAACG ATAACCCGCC GGTGTTCCCA GCAACACAAA 
     1441 AGAACCTGTC CATCGCGGAA TCCAGGCCGC TTGACTCTCG GTTTCCACTA GAGGGCGCCT 
     1501 CGGATGCAGA TATCGGGGAG AACGCCCTGC TCACTTACAG ACTGAGCCCA AATGAATACT 
     1561 TTTCTCTGGA AAAACCACCT GATGACGAGC TGGTAAAAGG TCTTGGGCTT ATATTACGGA 
     1621 AATCTTTAGA CAGAGAAGAA GCTCCGGAGA TTTTTTTAGT GCTCACAGCC ACTGATGGAG 
     1681 GCAAACCCGA GTTGACTGGC ACCGTTCAGT TACTCATCAC AGTACTGGAT GCCAATGACA 
     1741 ATGCCCCAGC TTTTGACAGA ACCATTTATA AGGTGAGATT ACTAGAAAAT GTTCCTAATG 
     1801 GAACATTGGT AATTAAACTT AACGCCTCAG ATTTAGACGA AGGATTGAAT GGGGACATTG 
     1861 TTTATTCATT CTCAAATGAT ATTTCGCCAA ATGTGAAATC CAAGTTTCAC ATAGATCCAA 
     1921 TTACTGGACA AATTATTGTA AAGGGATATA TTGACTTTGA AGAAAGCAAA TCCTATGAAA 
     1981 TTATTGTAGA GGGCATTGAT AAGGGACAGC TCCCACTTTC TGGCCATTGT AGAGTTATTG 
     2041 TGGAAGTAGA AGACAACAAC GATAATGTCC CAGATTTGGA ATTCAAGTCT TTATCACTTC 
     2101 CAATTAGAGA GGACGCTCCA CTGGGTACAG TCATCGCCCT GATCAGCGTG TCCGACAAAG 
     2161 ACATGGGTGT CAATGGGCTG GTCACCTGCT CCTTGACGTC CCACGTCCCC TTCAAGCTGG 
     2221 TGTCCACCTT CAAGAATTAC TACTCGTTGG TGCTGGACAG TGCCCTGGAC CGCGAGAGCG 
     2281 TGTCAGCCTA TGAGCTGGTG GTGACCGCGC GAGACGGGGG CTCGCCTTCG CTGTGGGCCA 
     2341 CGGCCAGTGT TTCTGTGGAG GTGGCTGATG TGAACGACAA CGCTCCGGCG TTCGCGCAGC 
     2401 CCGAGTACAC AGTGTTCGTG AAGGAGAACA ACCCGCCGGG CTGCCACATC TTCACTGTGT 
     2461 CTGCGTGGGA CGCGGACGCG CAGGAGAACG CGCTGGTGTC CTACTCGCTG GTAGAGCGGC 
     2521 GGGTAGGGGA GCGCGCGCTG TCGAGCTACG TTTCGGTGCA TGCGGAGAGC GGCAAGGTGT 
     2581 ACGCGCTGCA GCCGCTGGAC CACGAGGAGC TAGAGCTGCT GCAGTTTCAG GTGACCGCTC 
     2641 GCGATGCCGG CGTGCCACCT CTGGGCAGCA ACGTGACGCT GCAGGTGTTC GTGCTGGACG 
     2701 AAAACGACAA CGCGCCAGCA CTGCTAGCGC CTCGGGCGGG TGGCACTGGT GGCGCAGTGA 
     2761 GCGAGCTGGT GCCATGGTCG GTGGGTGTGG GCCACGTGGT GGCAAAGGTG CGCGCGGTGG 
     2821 ATGCTGACTC GGGCTACAAC GCGTGGCTTT CGTACGAGCT GCAGCCGGGG ACTGGTGGCG 
     2881 CGCGCATCCC GTTCCGCGTG GGGCTGTACA CTGGCGAGAT CAGCACAACG CGTGCCCTGG 
     2941 ACGAAACGGA CGCTCCGCGC CACCGCCTAC TGGTACTGGT GAAGGACCAC GGCGAGCCCG 
     3001 CGCTGACGGC CACGGCCACT GTGCTGGTGT CACTTGTGGA GAGTGGACAG GCGCCAAAGG 
     3061 CCTCCTCACG GGCGTTGGTG GGCGCTGTGG GTCCCGATGC TGCGCTGGTG GATGTCAACG 
     3121 TATACCTGAT CATTGCCATC TGCGCGGTGT CCAGCCTTTT GGTGCTCACG CTGCTGCTGT 
     3181 ACACCGCGCT GCGGTGCTCT GCGCTGCCCA CCGAGGGCGC GTGCGCTCCG GGCAAGCCCA 
     3241 CGCTGGTGTG CTCCAGTGCG GTGGGGAGCT GGTCATACTC GCAGCAGAGG AGGCCGAGGG 
     3301 TGTGCTCTGG TGAGGGCCCA CCCAAGACCG ACCTCATGGC CTTCAGCCCC AGTTTACCTG 
     3361 ACTCTAGGGA CAGAGAAGAT CAGCTGCAGA CAACTGAGGA ATCCTTTGCA AAGCCACGAC 
     3421 AGCCCAACCC TGACTGGCGT TACTCTGCCT CCCTGAGAGC AGGCATGCAC AGCTCTGTGC 
     3481 ACCTAGAGGA GGCTGGCATT CTACGGGCTG GTCCAGGAGG GCCTGATCAG CAGTGGCCAA 
     3541 CAGTATCCAG TGCAACACCA GAACCAGAGG CAGGAGAAGT GTCCCCTCCA GTCGGTGCGG 
     3601 GTGTCAACAG CAACAGCTGG ACCTTTAAAT ACGGACCAGG CAACCCCAAA CAATCCGGTC 
     3661 CCGGTGAGTT GCCCGACAAA TTCATTATCC CAGGATCTCC TGCAATCATC TCCATCCGGC 
     3721 AGGAGCCTAC TAACAGCCAA ATTGACAAAA GTGACTTCAT AACCTTCGGC AAAAAGGAGG 
     3781 AGACCAAGAA AAAGAAGAAA AAGAAGAAGG GTAACAAGAC CCAGGAGAAA AAAGAGAAAG 
     3841 GGAACAGCAC GACTGACAAC AGTGACCAGA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     3901 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3961 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4021 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4081 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4141 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4201 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4261 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4321 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4381 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4441 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4501 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4561 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4621 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4681 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4741 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4801 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4861 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4921 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4981 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5041 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5101 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5161 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5221 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5281 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5341 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5401 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5461 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5521 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5581 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5641 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5701 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5761 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5821 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5881 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5941 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6001 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6061 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6121 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6181 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6241 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6301 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6361 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6421 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6481 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6541 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6601 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6661 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6721 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6781 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6841 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6901 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6961 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7021 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7081 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7141 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7201 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7261 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7321 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7381 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7441 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7501 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7561 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7621 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7681 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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