LOCUS       RC214827                9498 bp    DNA     circular  SYN 
DEFINITION  ZFYVE16 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 16 (ZFYVE16), transcript variant 1
ACCESSION   NM_014733
VERSION     NM_014733.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5655..5746 
                     /label="Myc-DDK"  
     misc_feature    5776..6361 
                     /label="PolyA signal"  
     misc_feature    5833..5852 
                     /label="Restriction Enzyme"  
     misc_feature    6637..7309 
                     /label="ColE1"  
     misc_feature    7739..8533 
                     /label="Kan/Neo"  
     misc_feature    8568..8870 
                     /label="SV40 ori"  
     misc_feature    8956..9433 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5637..5643 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5637 
                     /label="ZFYVE16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAGTTAT TTTAAAGCAG CTGTCAGTGA CTTGGACAAA CTCCTTGATG 
     1081 ATTTTGAACA GAACCCAGAT GAACAAGATT ATCTCCAAGA TGTACAAAAT GCATATGATT 
     1141 CTAACCACTG CTCAGTTTCT TCAGAGTTGG CTTCCTCACA GCGAACTTCA TTGCTCCCAA 
     1201 AAGACCAAGA GTGCGTTAAT AGTTGTGCCT CATCAGAAAC AAGCTATGGA ACAAATGAGA 
     1261 GTTCCCTGAA TGAAAAAACA CTCAAGGGAC TTACTTCTAT ACAAAATGAA AAAAATGTAA 
     1321 CAGGACTTGA TCTTCTTTCT TCTGTGGATG GTGGTACTTC AGATGAAATC CAGCCGTTAT 
     1381 ATATGGGACG ATGTAGTAAA CCTATCTGTG ATCTGATAAG TGACATGGGT AACTTAGTTC 
     1441 ATGCAACCAA TAGTGAAGAA GATATTAAAA AATTATTGCC AGATGATTTT AAGTCTAATG 
     1501 CAGATTCCTT GATTGGATTG GATTTATCTT CAGTGTCAGA TACTCCCTGT GTTTCTTCAA 
     1561 CAGACCATGA TAGTGATACT GTCAGAGAAC AACAGAATGA TATCAGTTCT GAATTACAAA 
     1621 ATAGAGAAAT CGGAGGAATC AAAGAATTGG GTATAAAAGT AGATACAACA CTTTCAGATT 
     1681 CCTATAATTA CAGTGGAACA GAAAATTTAA AAGATAAAAA GATCTTTAAT CAGTTAGAAT 
     1741 CAATTGTTGA TTTTAACATG TCATCTGCTT TGACTCGACA AAGTTCCAAA ATGTTTCATG 
     1801 CCAAAGACAA GCTACAACAC AAGAGCCAGC CATGTGGATT ACTAAAAGAT GTTGGCTTAG 
     1861 TAAAAGAGGA AGTAGATGTG GCAGTCATAA CTGCCGCAGA ATGTTTAAAA GAAGAGGGCA 
     1921 AGACAAGTGC TTTGACCTGC AGCCTTCCGA AAAATGAAGA TTTATGCTTA AATGATTCAA 
     1981 ATTCAAGAGA TGAAAATTTC AAATTACCTG ACTTTTCCTT TCAGGAAGAT AAGACTGTTA 
     2041 TAAAACAATC TGCACAAGAA GACTCAAAAA GTTTAGACCT TAAGGATAAT GATGTAATCC 
     2101 AAGATTCCTC TTCAGCTTTA CATGTTTCCA GTAAAGATGT GCCGTCCTCA TTGTCCTGTC 
     2161 TTCCTGCGTC TGGGTCTATG TGTGGATCAT TAATTGAAAG TAAAGCACGG GGTGATTTTT 
     2221 TACCTCAGCA TGAACATAAA GATAATATAC AAGATGCAGT GACTATACAT GAAGAAATAC 
     2281 AGAACAGTGT TGTTCTAGGT GGGGAACCAT TCAAAGAGAA TGATCTTTTG AAACAGGAAA 
     2341 AATGTAAAAG CATACTCCTT CAGTCATTAA TTGAAGGGAT GGAAGACAGA AAGATAGATC 
     2401 CTGACCAGAC AGTAATCAGA GCTGAGTCTT TGGATGGTGG TGACACCAGT TCTACAGTTG 
     2461 TAGAATCTCA AGAGGGGCTT TCTGGCACTC ATGTCCCAGA GTCTTCTGAT TGTTGTGAAG 
     2521 GTTTTATTAA TACTTTTTCA AGCAATGATA TGGATGGGCA AGACTTAGAT TACTTTAATA 
     2581 TTGATGAAGG CGCAAAAAGT GGCCCACTAA TTAGTGATGC TGAACTTGAT GCCTTTCTGA 
     2641 CAGAACAGTA TCTTCAGACC ACTAACATAA AGTCTTTTGA AGAAAATGTA AATGACTCTA 
     2701 AATCGCAAAT GAATCAGATA GATATGAAAG GCTTAGATGA TGGAAACATC AATAATATAT 
     2761 ATTTCAATGC AGAAGCAGGA GCTATTGGGG AAAGTCATGG TATTAATATA ATTTGTGAAA 
     2821 CAGTTGATAA ACAAAATACA ATAGAAAATG GCCTTTCTTT AGGAGAAAAA AGCACTATTC 
     2881 CAGTTCAACA AGGGTTACCT ACCAGTAAGT CTGAGATTAC AAATCAATTA TCAGTCTCTG 
     2941 ATATTAACAG TCAATCTGTT GGAGGGGCCA GACCTAAGCA ATTGTTTAGC CTTCCATCAA 
     3001 GAACAAGGAG TTCAAAGGAC CTGAATAAGC CAGATGTTCC AGATACAATA GAAAGTGAAC 
     3061 CCAGCACAGC AGATACCGTT GTTCCAATCA CTTGTGCTAT AGATTCTACA GCTGATCCAC 
     3121 AGGTTAGCTT CAACTCTAAT TACATTGATA TAGAAAGTAA TTCTGAAGGT GGATCTAGTT 
     3181 TCGTAACTGC AAATGAAGAT TCTGTACCTG AAAACACTTG CAAAGAAGGC TTGGTTTTGG 
     3241 GCCAGAAACA GCCTACTTGG GTTCCTGATT CAGAAGCTCC AAACTGTATG AACTGCCAAG 
     3301 TCAAATTTAC TTTTACCAAA CGGCGACACC ATTGCCGAGC ATGTGGGAAA GTATTTTGTG 
     3361 GTGTCTGTTG TAATAGGAAG TGTAAACTGC AATATCTAGA AAAGGAAGCA AGAGTATGTG 
     3421 TAGTCTGCTA TGAAACTATT AGTAAAGCTC AGGCATTTGA AAGGATGATG AGTCCAACTG 
     3481 GTTCTAATCT TAAGTCTAAT CATTCTGATG AATGTACTAC TGTCCAGCCT CCTCAGGAGA 
     3541 ACCAAACATC CAGTATACCT TCACCAGCAA CTTTGCCAGT CTCAGCACTT AAACAACCAG 
     3601 GTGTTGAAGG ACTATGTTCC AAAGAACAGA AGAGAGTATG GTTTGCAGAT GGTATATTGC 
     3661 CCAATGGTGA AGTTGCAGAT ACAACAAAAT TATCATCTGG AAGTAAAAGA TGTTCTGAAG 
     3721 ACTTTAGTCC TCTCTCACCT GATGTGCCTA TGACAGTAAA CACAGTGGAT CATTCCCATT 
     3781 CTACTACAGT GGAAAAGCCA AACAATGAGA CAGGAGATAT TACAAGAAAT GAGATAATTC 
     3841 AGAGTCCTAT TTCTCAGGTT CCATCAGTGG AAAAATTGTC TATGAACACA GGAAATGAGG 
     3901 GGTTACCTAC TTCTGGTTCA TTTACACTAG ATGATGATGT TTTTGCAGAA ACTGAAGAAC 
     3961 CATCTAGTCC TACTGGTGTC TTAGTTAACA GCAATTTACC TATTGCTAGT ATTTCAGATT 
     4021 ATAGGTTACT GTGTGATATT AACAAGTATG TCTGCAATAA GATTAGTCTT CTACCTAATG 
     4081 ATGAGGACAG TTTGCCCCCA CTTCTGGTTG CATCTGGAGA AAAGGGATCA GTGCCTGTAG 
     4141 TAGAAGAACA TCCATCTCAT GAGCAGATCA TTTTGCTTCT TGAAGGTGAA GGCTTTCATC 
     4201 CTGTTACATT TGTCCTAAAT GCTAATCTAC TCGTGAATGT CAAATTCATA TTTTATTCCT 
     4261 CAGACAAATA TTGGTACTTT TCAACCAATG GATTGCATGG CTTGGGACAG GCAGAAATTA 
     4321 TTATTCTATT GTTATGTTTG CCAAATGAAG ATACTATTCC TAAGGACATC TTCAGACTAT 
     4381 TTATCACCAT ATATAAGGAT GCTCTAAAAG GAAAATACAT AGAAAACTTG GACAATATTA 
     4441 CCTTTACTGA GAGTTTTCTC AGTAGCAAGG ATCACGGAGG ATTCCTGTTT ATTACACCTA 
     4501 CTTTTCAGAA ACTTGATGAT CTCTCATTAC CAAGTAATCC TTTTCTTTGT GGAATTCTTA 
     4561 TCCAGAAGCT TGAGATTCCC TGGGCAAAGG TTTTTCCTAT GCGTTTAATG TTGAGATTGG 
     4621 GTGCAGAATA TAAAGCATAT CCTGCTCCTC TAACAAGCAT CAGAGGCCGA AAACCTCTTT 
     4681 TTGGAGAAAT AGGACACACT ATTATGAACT TACTTGTTGA CCTTCGAAAT TACCAGTATA 
     4741 CCTTGCATAA TATAGATCAA CTGTTGATTC ATATGGAAAT GGGAAAAAGC TGCATAAAAA 
     4801 TACCACGGAA AAAGTACAGT GATGTAATGA AAGTACTAAA TTCTTCCAAT GAGCATGTCA 
     4861 TTAGCATTGG AGCAAGTTTC AGTACAGAAG CAGATTCTCA TCTAGTCTGT ATACAGAATG 
     4921 ATGGAATTTA TGAAACACAG GCCAACAGTG CCACTGGCCA TCCTAGAAAA GTGACAGGTG 
     4981 CAAGTTTTGT GGTATTCAAT GGAGCTCTAA AAACATCTTC AGGATTTCTT GCTAAGTCCA 
     5041 GCATAGTTGA AGATGGCTTA ATGGTACAAA TAACTCCAGA GACCATGAAT GGCTTGCGGC 
     5101 TAGCTTTACG AGAACAGAAA GACTTTAAAA TTACATGTGG GAAAGTTGAT GCAGTAGACC 
     5161 TGAGAGAATA CGTGGATATC TGCTGGGTAG ATGCTGAAGA AAAAGGAAAC AAAGGAGTTA 
     5221 TCAGTTCAGT GGATGGAATA TCATTACAAG GATTTCCAAG TGAAAAAATA AAACTGGAAG 
     5281 CAGATTTTGA AACCGATGAG AAGATTGTAA AATGTACCGA GGTGTTCTAC TTTCTAAAGG 
     5341 ACCAGGATTT ATCTATTTTA TCAACTTCTT ATCAGTTTGC AAAAGAAATA GCCATGGCTT 
     5401 GTAGTGCTGC GCTGTGCCCT CACCTGAAAA CTCTAAAAAG TAATGGGATG AATAAAATTG 
     5461 GACTCAGAGT TTCCATTGAC ACTGATATGG TTGAATTTCA GGCAGGATCT GAAGGCCAAC 
     5521 TTCTGCCTCA GCATTATCTA AATGATCTTG ATAGTGCTCT GATACCTGTG ATCCATGGTG 
     5581 GGACCTCCAA CTCTAGTTTA CCATTAGAAA TAGAATTAGT GTTTTTCATT ATAGAACATC 
     5641 TTTTTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5701 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5761 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5821 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5881 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5941 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     6001 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     6061 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     6121 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     6181 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6241 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6301 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6361 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6421 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6481 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6541 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6601 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6661 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6721 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6781 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6841 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6901 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6961 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     7021 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     7081 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7141 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7201 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7261 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7321 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7381 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7441 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7501 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7561 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7621 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7681 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7741 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7801 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7861 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7921 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7981 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     8041 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     8101 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     8161 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     8221 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8281 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8341 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8401 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8461 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8521 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8581 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8641 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8701 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8761 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8821 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8881 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8941 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9001 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     9061 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     9121 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     9181 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9241 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9301 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9361 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9421 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9481 AAATTTAACG CGAATTTT
//