LOCUS       RC214806                8310 bp    DNA     circular  SYN 
DEFINITION  FHDC1 (Myc-DDK-tagged)-Human FH2 domain containing 1 (FHDC1)
ACCESSION   NM_033393
VERSION     NM_033393.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4467..4558 
                     /label="Myc-DDK"  
     misc_feature    4588..5173 
                     /label="PolyA signal"  
     misc_feature    4645..4664 
                     /label="Restriction Enzyme"  
     misc_feature    5449..6121 
                     /label="ColE1"  
     misc_feature    6551..7345 
                     /label="Kan/Neo"  
     misc_feature    7380..7682 
                     /label="SV40 ori"  
     misc_feature    7768..8245 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4449..4455 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4449 
                     /label="FHDC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATGTTATG AATTGTGTCT CCTTGGTCAG TGATAAAGAA AATGGGAATA 
     1081 TTGCCACAGC ACCTGGATTC ATGATTGGGC AGACACCTCC TCCAGCACCT CCTCCACCTC 
     1141 CTCCTCCACC CCCTCCATCT CCACCATGTT CATGTTCAAG GGAAGAGTGT CCTTCCTCCC 
     1201 CTCCTCCACC CCCACCACCT CCACTTCCTG GGGAGCCTCC CATCCCACCT CCCCCACCAG 
     1261 GCCTACCCCC AACTACTCAC ATGAACGGCT ACAGCCACCT TGGTAAGAAA AAGCGGATGA 
     1321 GAAGCTTTTT TTGGAAAACT ATTCCGGAGG AGCAAGTTCG AGGCAAAACC AACATCTGGA 
     1381 CCTTGGCAGC CAGGCAGGAA CATCACTACC AAATTGATAC AAAGACCATT GAGGAGCTCT 
     1441 TTGGGCAGCA GGAAGACACC ACCAAGTCTT CCCTTCCTAG GAGAGGAAGA ACTTTAAATT 
     1501 CATCCTTCAG AGAAGCTCGA GAAGAGATTA CTATTTTGGA TGCAAAACGG AGCATGAACA 
     1561 TTGGGATATT TCTTAAGCAA TTTAAGAAGT CTCCTCGGTC CATTGTAGAA GATATTCATC 
     1621 AAGGAAAAAG TGAGCATTAT GGATCAGAGA CCTTGCGAGA ATTTCTTAAG TTTTTGCCAG 
     1681 AGTCAGAAGA GGTAAAGAAG TTAAAAGCGT TTAGTGGCGA CGTGTCGAAG CTGTCTCTGG 
     1741 CAGATTCCTT TCTGTATGGC TTAATTCAGG TGCCAAACTA TTCACTTCGG ATTGAAGCCA 
     1801 TGGTGCTAAA GAAGGAATTT CTACCTTCTT GCTCTTCTCT ATATACAGAT ATAACAGTTT 
     1861 TAAGAACTGC TATAAAAGAA CTGATGTCAT GTGAAGAGCT ACATTCAATA TTACACTTGG 
     1921 TGCTCCAGGC TGGGAATATC ATGAATGCAG GAGGGTATGC CGGCAATGCA GTAGGATTTA 
     1981 AACTGTCTTC TTTGCTCAAA TTGGCAGACA CAAAAGCAAA CAAACCTGGG ATGAATCTCC 
     2041 TGCACTTTGT TGCACAGGAA GCCCAAAAGA AAGATACCAT TCTTCTAAAC TTTTCAGAAA 
     2101 AATTGCATCA TGTTCAGAAG ACTGCTAGAT TATCTCTGGA GAACACGGAG GCAGAACTGC 
     2161 ACTTGCTGTT TGTCAGGACA AAATCACTAA AAGAAAACAT CCAGCGGGAT GGTGAACTTT 
     2221 GTCAGCAGAT GGAAGATTTT CTTCAGTTTG CCATAGAAAA GCTGAGGGAA CTGGAATGCT 
     2281 GGAAACAAGA GCTCCAGGAT GAGGCCTACA CCCTTATAGA TTTTTTCTGT GAAGACAAAA 
     2341 AAACCATGAA ACTGGATGAA TGCTTTCAGA TATTTAGAGA TTTCTGTACC AAATTCAACA 
     2401 AAGCAGTTAA GGACAACCAC GACCGGGAGG CGCAGGAGCT GAGGCAGCTG CAGAGGCTGA 
     2461 AGGAGCAGGA GCAGAAGCAG CGCTCCTGGG CAACTGGGGA GCTGGGGGCA TTTGGCCGGA 
     2521 GCAGCAGTGA GAATGATGTG GAGCTGCTGA CCAAGAAGGG TGCAGAGGGC CTGCTCCCTT 
     2581 TCCTGCACCC CAGGCCCATC AGCCCCTCCA GCCCCTCCTA CCGGCCCCCG AACACCCGCC 
     2641 GCTCCCGCCT CTCCCTGGGT CCCTCTGCTG ACCGGGAGCT GCTGACCTTC TTGGAGAGCT 
     2701 CCACCGGCAG CCCTGAGGAG CCCAATAAGT TCCACAGCCT GCCCCGGAGC AGCCCCCGGC 
     2761 AGGCCCGGCC CACGATAGCC TGCCTGGAGC CTGCAGAAGT GAGGCACCAG GACTCCAGCT 
     2821 TTGCACACAA ACCTCAGGCC TCGGGGGGCC AGGAGGAGGC CCCCAACCCA CCCTCAGCAC 
     2881 AGGCGCACCA GCTTGCAGCC GCCCAGCCTG AGAACCATGC CTCTGCCTTC CCCAGAGCTC 
     2941 GGTGCCAGGG CGTCAGTGTC CTCCGAAAGA GGTACAGTGA GCCGGTGAGC CTGGGCTCAG 
     3001 CACAGTCCCC TCCTCTCTCG CCATTGGCTC TGGGAATTAA GGAGCATGAG CTGGTGACAG 
     3061 GGCTGGCCCA GTTCAACCTC CAGGGTTCCC AGGGCATGGA GGAGACCTCC CAGCTGACTC 
     3121 TGAGTGACTT CAGCCCGATG GAGCTAGAGT CTGTGGGGCA TAGGGGCCCG CAGTCCCTCA 
     3181 GTGCCAGCAG CAGCAGCCTG ACACCCATGG GCAGAGATGC CCTGGGGAGT CTCAGCCCAG 
     3241 CGCTGGAGGA TGGCAAGGCT GCCCCCGATG AGCCTGGAAG TGCAGCTTTG GGATCTGTGG 
     3301 GTAGCAGCGA CCCTGAGAAC AAAGATCCTA GACCTCTGTT CTGCATCTCG GACACCACCG 
     3361 ACTGCTCACT GACCCTGGAC TGCTCAGAGG GAACCGACTC CAGACCCAGA GGCGGGGACC 
     3421 CGGAGGAAGG CGGGGAAGGG GATGGCTCCA TGTCCTCTGG GGTTGGAGAA ATGGGGGACA 
     3481 GCCAAGTCTC CTCCAACCCT ACATCCAGCC CCCCTGGGGA GGCTCCTGCC CCCGTCTCTG 
     3541 TGGATAGTGA GCCCAGCTGC AAGGGCGGCC TGCCCAGGGA CAAACCCACC AAAAGGAAAG 
     3601 ATGTTGTAGC ACCAAAGAGA GGCTCCCTGA AAGAGGCGTC TCCCGGGGCC TCCAAGCCCG 
     3661 GGAGCGCCCG GCGGAGCCAG GGGGCAGTGG CCAAGTCTGT GCGGACCCTG ACCGCCTCAG 
     3721 AGAACGAGAG CATGCGCAAG GTCATGCCCA TCACCAAGTC CAGCAGAGGC GCCGGCTGGA 
     3781 GGCGACCAGA GCTGTCATCC CGGGGGCCCT CCCAGAATCC CCCCAGCAGC ACAGATACTG 
     3841 TGTGGTCACG CCAGAACTCC GTGCGGAGGG CCTCCACAGG CGCCGAAGAG CAGAGGCTGC 
     3901 CGCGGGGGAG CAGCGGCTCC AGCAGCACCC GTCCGGGGAG GGACGTTCCC CTGCAGCCCA 
     3961 GGGGTTCTTT CAAGAAGCCC AGTGCCAAAC CACTCAGGAA CCTCCCCAGA CAGAAGCCTG 
     4021 AGGAAAATAA GACCTGCCGC GCCCACTCCG AGGGCCCTGA GAGTCCCAAA GAAGAGCCCA 
     4081 AGACCCCGTC AGTGCCCAGC GTCCCCCACG AACTACCCCG TGTCCCGAGC TTTGCCCGGA 
     4141 ACACAGTGGC CTCCTCCTCT CGAAGCATGA GAACAGATCT TCCTCCCGTG GCCAAAGCCC 
     4201 CCGGCATCAC TCGGACAGTG TCGCAGCGGC AGCTGAGGGT GAAAGGGGAC CCCGAGGATG 
     4261 CCGCTCCCAA GGACAGCAGC ACTTTGAGGC GAGCCAGCAG TGCCCGGGCC CCCAAGAAGC 
     4321 GCCCAGAGTC TGCGGAGGGT CCCAGTGCCA ACACGGAGGC CCCTCTGAAG GCCAGAGGGG 
     4381 CTGGGGAAAG GGCCTCCCTC CGTCGGAAGG ACTCCAGTCG GACCACGCTG GGGAGAATCC 
     4441 TCAATCCCTT ACGGAAGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4501 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4561 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4621 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4681 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4741 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4801 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4861 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4921 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4981 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5041 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5101 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5161 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5221 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5281 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5341 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5401 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5461 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5521 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5581 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5641 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5701 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5761 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5821 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5881 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5941 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6001 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6061 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6121 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6181 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6241 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6301 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6361 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6421 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6481 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6541 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6601 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6661 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6721 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6781 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6841 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6901 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6961 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7021 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7081 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7141 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7201 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7261 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7321 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7381 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7441 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7501 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7561 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7621 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7741 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7801 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7861 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7921 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7981 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8041 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8101 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8161 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8221 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8281 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//