LOCUS       RC214763                7962 bp    DNA     circular  SYN 
DEFINITION  PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2
ACCESSION   NM_015107
VERSION     NM_015107.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4117..4208 
                     /label="Myc-DDK"  
     misc_feature    4238..4823 
                     /label="PolyA signal"  
     misc_feature    4295..4314 
                     /label="Restriction Enzyme"  
     misc_feature    5099..5771 
                     /label="ColE1"  
     misc_feature    6201..6995 
                     /label="Kan/Neo"  
     misc_feature    7030..7332 
                     /label="SV40 ori"  
     misc_feature    7418..7895 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4092..4099 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4092 
                     /label="PHF8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGGTG CCGGTGTATT GCCTCTGCCG GCTGCCTTAC GATGTGACCC 
     1081 GCTTCATGAT CGAGTGTGAC ATGTGCCAGG ACTGGTTTCA TGGCAGTTGT GTTGGTGTTG 
     1141 AAGAGGAGAA GGCTGCTGAC ATTGACCTCT ACCACTGCCC CAACTGTGAA GTCTTGCATG 
     1201 GGCCCTCCAT TATGAAAAAA CGCCGTGGAT CTTCAAAGGG GCATGATACA CACAAGGGGA 
     1261 AACCAGTGAA GACCGGGAGC CCTACGTTCG TCAGAGAGCT CCGGAGTAGG ACTTTTGACA 
     1321 GCTCAGATGA AGTGATTCTG AAGCCCACTG GAAATCAACT GACCGTGGAA TTCCTGGAAG 
     1381 AAAATAGCTT CAGTGTGCCC ATCCTGGTCC TGAAGAAGGA TGGGTTGGGC ATGACGCTGC 
     1441 CCTCGCCATC ATTCACTGTG AGGGATGTTG AACACTATGT TGGTTCTGAC AAAGAGATTG 
     1501 ATGTGATTGA TGTGACCCGC CAGGCTGACT GCAAGATGAA GCTTGGTGAT TTTGTGAAAT 
     1561 ACTATTACAG CGGGAAGAGG GAGAAAGTCC TCAATGTCAT TAGTTTGGAA TTCTCTGATA 
     1621 CCAGACTTTC TAACCTTGTG GAGACACCGA AGATTGTTCG AAAGCTGTCA TGGGTCGAAA 
     1681 ACTTGTGGCC AGAGGAATGT GTCTTTGAGA GACCCAATGT ACAGAAGTAC TGCCTCATGA 
     1741 GTGTGCGAGA TAGCTATACA GACTTTCACA TTGACTTTGG TGGCACCTCT GTCTGGTACC 
     1801 ATGTACTCAA GGGTGAAAAG ATCTTCTACC TGATCCGCCC AACAAATGCC AATCTGACTC 
     1861 TCTTTGAGTG CTGGAGCAGT TCCTCTAATC AGAATGAGAT GTTCTTTGGG GACCAGGTGG 
     1921 ACAAGTGCTA CAAGTGTTCC GTGAAGCAAG GACAGACACT TTTCATTCCC ACAGGGTGGA 
     1981 TCCATGCTGT GCTGACGCCT GTGGACTGCC TTGCCTTTGG AGGGAACTTC TTACACAGCC 
     2041 TTAACATCGA GATGCAGCTC AAAGCCTATG AGATTGAGAA GCGGCTGAGC ACAGCAGACC 
     2101 TCTTCAGATT CCCCAACTTT GAGACCATCT GTTGGTATGT GGGAAAGCAC ATCCTGGACA 
     2161 TCTTTCGCGG TTTGCGAGAG AACAGGAGAC ACCCTGCCTC CTACCTGGTC CATGGTGGCA 
     2221 AAGCCTTGAA CTTGGCCTTT AGAGCCTGGA CAAGGAAAGA AGCTCTGCCA GACCATGAGG 
     2281 ATGAGATCCC GGAGACAGTG CGAACCGTAC AGCTCATTAA AGATCTGGCC AGGGAGATCC 
     2341 GCCTGGTGGA AGACATCTTC CAACAGAACG TTGGGAAGAC GAGCAATATC TTTGGGCTGC 
     2401 AGAGGATCTT CCCAGCCGGC TCCATTCCCC TAACCAGGCC AGCCCATTCC ACTTCAGTGT 
     2461 CCATGTCCAG GCTGTCACTG CCCTCCAAAA ATGGTTCAAA GAAGAAAGGC CTGAAGCCCA 
     2521 AGGAACTCTT CAAGAAGGCA GAGCGAAAGG GCAAGGAGAG TTCAGCCTTG GGGCCTGCTG 
     2581 GCCAGTTGAG CTATAATCTC ATGGACACAT ACAGTCATCA GGCACTGAAG ACAGGCTCTT 
     2641 TCCAGAAAGC AAAGTTCAAC ATCACTGGTG CCTGCTTGAA TGACTCAGAT GACGACTCAC 
     2701 CAGACTTGGA CCTTGATGGA AATGAGAGCC CATTGGCCCT ATTGATGTCT AACGGCAGTA 
     2761 CGAAAAGGGT GAAGAGTTTA TCCAAATCTC GGCGAACCAA GATAGCAAAG AAGGTAGACA 
     2821 AGGCTAGGCT GATGGCAGAA CAGGTGATGG AAGACGAATT TGACTTGGAT TCAGATGATG 
     2881 AGCTGCAGAT TGACGAGAGA TTGGGAAAGG AGAAGGCGAC CCTGATAATA AGACCAAAAT 
     2941 TTCCCCGGAA ATTGCCCCGT GCGAAGCCTT GCTCTGACCC CAACCGAGTT CGTGAACCAG 
     3001 GAGAAGTTGA GTTTGACATT GAGGAGGACT ATACAACAGA TGAGGACATG GTGGAAGGGG 
     3061 TTGAAGGCAA GCTTGGGAAT GGTAGTGGCG CTGGTGGCAT TCTTGATCTG CTCAAGGCCA 
     3121 GCAGGCAGGT GGGGGGACCT GACTATGCTG CCCTCACCGA GGCCCCAGCT TCTCCCAGCA 
     3181 CTCAGGAGGC CATCCAGGGC ATGCTGTGCA TGGCCAACCT GCAGTCCTCA TCGTCCTCAC 
     3241 CGGCTACCTC TAGCCTGCAG GCCTGGTGGA CTGGGGGACA GGATCGAAGC AGTGGGAGCT 
     3301 CCAGCAGTGG GCTGGGCACA GTGTCTAACA GTCCTGCTTC CCAGCGCACC CCAGGGAAGC 
     3361 GGCCCATCAA GCGGCCAGCA TACTGGAGAA CCGAGAGCGA GGAGGAGGAG GAGAACGCCA 
     3421 GTCTGGATGA ACAGGACAGC TTGGGAGCGT GCTTCAAGGA TGCAGAGTAT ATCTATCCTT 
     3481 CACTGGAGTC TGATGATGAT GACCCTGCTT TGAAATCTCG ACCCAAGAAA AAGAAGAATT 
     3541 CAGATGATGC TCCATGGAGT CCTAAAGCCC GCGTGACCCC AACTCTGCCG AAGCAGGACC 
     3601 GTCCTGTGCG TGAGGGGACC CGGGTAGCCT CTATTGAGAC AGGTTTGGCT GCAGCAGCTG 
     3661 CAAAGCTGGC CCAGCAGGAG CTACAGAAGG CCCAAAAGAA GAAATATATC AAGAAGAAGC 
     3721 CTTTGCTGAA GGAGGTAGAA CAGCCTCGCC CTCAAGACTC CAATCTCAGT CTGACAGTAC 
     3781 CAGCCCCCAC TGTGGCTGCC ACACCACAAC TTGTCACCTC CTCCTCACCC CTGCCTCCTC 
     3841 CTGAGCCTAA ACAAGAGGCC CTGTCAGGAA GTCTCGCTGA CCATGAGTAC ACCGCTCGTC 
     3901 CCAATGCCTT TGGCATGGCC CAGGCAAACC GCAGCACCAC ACCTATGGCC CCCGGTGTCT 
     3961 TCTTGACCCA GCGGCGCCCT TCAGTTGGCT CCCAGAGCAA TCAGGCAGGA CAAGGAAAGC 
     4021 GTCCCAAAAA GGGCCTGGCC ACAGCAAAGC AGAGACTCGG CCGTATCCTG AAAATCCACA 
     4081 GAAATGGCAA ACTACTTCTG AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4141 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4201 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4261 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4321 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4381 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4441 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4501 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4561 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4621 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4681 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4741 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4801 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4861 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4921 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4981 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5041 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5101 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5161 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5221 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5281 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5341 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5401 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5461 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5521 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5581 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5641 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5701 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5761 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5821 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5881 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5941 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6001 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6061 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6121 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6181 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6241 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6301 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6361 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6421 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6481 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6541 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6601 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6661 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6721 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6781 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6841 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6901 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6961 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7021 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7081 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7141 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7201 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7261 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7321 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7381 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7441 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7501 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7561 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7621 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7681 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7741 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7801 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7861 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7921 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//