LOCUS       RC214729                8325 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK-tagged)-Human myosin binding protein C, slow type (MYBPC1), transcript variant 2
ACCESSION   NM_206819
VERSION     NM_206819.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4482..4573 
                     /label="Myc-DDK"  
     misc_feature    4603..5188 
                     /label="PolyA signal"  
     misc_feature    4660..4679 
                     /label="Restriction Enzyme"  
     misc_feature    5464..6136 
                     /label="ColE1"  
     misc_feature    6566..7360 
                     /label="Kan/Neo"  
     misc_feature    7395..7697 
                     /label="SV40 ori"  
     misc_feature    7783..8260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4464..4470 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4464 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGAAAATGA AGTGCCAGCC CCAGCCCCAC 
     1081 CCCCGGAAGA ACCAAGTAAA GAGAAGGAGG CCGGAACTAC ACCAGCAAAA GATGAAGAGG 
     1141 AAGTCTCCCC GCCTAGCGCC TTGCCTCCAG GTTTGGGTAG TCGGGCCCTG GAGAGAAAAG 
     1201 ATTCAGACTG GACCCTTGTC GAAACTCCTC CTGGGGAGGA ACAAGCCAAG CAGAATGCCA 
     1261 ACTCCCAGCT GTCCATCTTG TTCATTGAAA AACCTCAAGG AGGAACAGTG AAAGTTGGTG 
     1321 AAGATATCAC CTTCATAGCC AAAGTCAAGG CTGAAGATCT TCTGAGAAAA CCCACTATCA 
     1381 AATGGTTCAA AGGAAAATGG ATGGACCTGG CCAGCAAAGC CGGGAAGCAC CTTCAGCTGA 
     1441 AGGAAACCTT TGAGAGGCAC AGTCGGGTGT ACACATTTGA GATGCAGATC ATCAAGGCCA 
     1501 AAGATAACTT TGCAGGAAAT TACAGATGCG AGGTCACCTA TAAGGATAAG TTTGACAGCT 
     1561 GTTCATTTGA TCTTGAAGTG CACGAATCTA CTGGGACTAC TCCAAACATT GACATCAGAT 
     1621 CTGCTTTCAA GAGAAGTGGA GAAGGTCAAG AGGATGCAGG AGAACTTGAC TTTAGTGGTC 
     1681 TCCTGAAACG TAGGGAGGTG AAGCAGCAGG AGGAAGAACC CCAGGTGGAC GTATGGGAGT 
     1741 TGCTGAAGAA CGCGAAACCC AGTGAGTACG AGAAGATCGC CTTCCAGTAT GGAATCACCG 
     1801 ACCTGCGCGG CATGCTCAAG CGACTCAAGC GCATGCGCAG AGAGGAGAAG AAGAGCGCAG 
     1861 CTTTTGCAAA AATTCTTGAT CCTGCATATC AGGTTGACAA AGGAGGCAGA GTGAGGTTTG 
     1921 TTGTGGAGCT GGCAGATCCA AAGTTGGAGG TGAAATGGTA TAAAAATGGT CAAGAAATTC 
     1981 GACCCAGTAC CAAATACATC TTTGAACACA AAGGATGCCA GAGAATCCTG TTTATCAATA 
     2041 ACTGTCAGAT GACAGATGAT TCAGAGTATT ATGTGACAGC CGGTGATGAG AAATGTTCCA 
     2101 CTGAGCTCTT CGTAAGAGAG CCTCCAATTA TGGTGACCAA ACAGCTGGAA GATACAACTG 
     2161 CTTATTGTGG GGAGAGAGTG GAATTAGAAT GTGAGGTGTC TGAAGATGAT GCCAATGTAA 
     2221 AATGGTTTAA GAATGGTGAA GAGATTATCC CTGGTCCAAA ATCAAGATAC CGAATTAGAG 
     2281 TTGAGGGTAA AAAACACATC TTGATCATAG AGGGAGCAAC AAAGGCTGAT GCTGCAGAAT 
     2341 ATTCAGTAAT GACAACAGGA GGACAATCAT CTGCTAAACT TAGTGTTGAC TTGAAACCTC 
     2401 TGAAGATTTT GACACCTCTG ACTGATCAGA CTGTAAATCT TGGAAAAGAA ATCTGCCTGA 
     2461 AGTGTGAAAT CTCTGAAAAC ATACCAGGAA AATGGACTAA AAATGGCCTA CCTGTTCAGG 
     2521 AGAGTGACCG TCTAAAGGTG GTTCACAAGG GAAGGATCCA CAAGTTAGTG ATAGCCAATG 
     2581 CCCTCACTGA AGATGAAGGT GATTATGTAT TTGCACCTGA TGCCTACAAT GTTACTCTGC 
     2641 CTGCCAAAGT TCATGTTATT GATCCTCCTA AGATCATCCT GGATGGTCTT GATGCTGACA 
     2701 ACACAGTGAC AGTGATTGCA GGAAACAAGC TTCGTCTTGA GATCCCCATC AGCGGAGAAC 
     2761 CACCTCCTAA AGCCATGTGG AGCCGGGGAG ATAAGGCTAT TATGGAAGGC AGTGGCCGGA 
     2821 TAAGAACAGA ATCTTACCCT GATAGCAGCA CTCTGGTCAT TGATATAGCT GAAAGAGATG 
     2881 ACTCTGGTGT TTACCACATC AATCTGAAAA ACGAAGCTGG AGAGGCACAT GCAAGCATCA 
     2941 AGGTTAAAGT TGTGGACTTC CCTGATCCTC CAGTGGCACC GACTGTGACA GAGGTGGGAG 
     3001 ATGACTGGTG TATCATGAAC TGGGAGCCTC CTGCCTACGA CGGAGGCTCT CCAATCCTAG 
     3061 GATATTTTAT TGAGAGGAAG AAGAAACAAA GCTCCAGGTG GATGAGGCTG AATTTTGATC 
     3121 TCTGCAAAGA AACAACTTTT GAGCCCAAGA AGATGATTGA AGGTGTGGCC TATGAGGTCC 
     3181 GCATCTTTGC AGTCAATGCC ATTGGCATCT CCAAGCCCAG TATGCCCTCC AGGCCTTTTG 
     3241 TTCCTTTGGC TGTAACAAGC CCTCCTACTC TTCTGACTGT GGACTCTGTC ACTGACACGA 
     3301 CTGTCACGAT GAGGTGGCGC CCCCCAGACC ACATTGGTGC AGCAGGTTTA GATGGCTATG 
     3361 TGCTAGAGTA TTGCTTTGAA GGAACTGAGG ACTGGATAGT TGCAAACAAA GATCTGATTG 
     3421 ACAAGACGAA GTTCACCATC ACAGGTCTGC CAACAGATGC AAAGATCTTT GTGCGTGTGA 
     3481 AGGCTGTTAA TGCAGCTGGT GCCAGCGAGC CCAAGTACTA TTCTCAGCCC ATTCTCGTGA 
     3541 AGGAAATCAT AGAACCTCCA AAGATTCGCA TTCCAAGACA CCTGAAGCAA ACCTATATCC 
     3601 GCAGAGTTGG AGAAGCTGTC AATCTGGTTA TACCTTTCCA GGGAAAACCA AGACCAGAAT 
     3661 TAACTTGGAA GAAGGATGGT GCAGAAATTG ATAAGAATCA AATAAACATT CGCAACTCTG 
     3721 AGACTGATAC AATCATATTT ATTAGAAAAG CAGAGAGGAG CCACTCTGGG AAATATGATC 
     3781 TGCAAGTCAA AGTGGACAAA TTCGTGGAGA CCGCATCAAT TGACATCCAG ATCATTGACC 
     3841 GTCCAGGTCC ACCCCAAATT GTGAAGATTG AGGATGTCTG GGGAGAAAAT GTCGCTCTCA 
     3901 CATGGACTCC ACCAAAGGAT GATGGAAATG CTGCTATCAC AGGCTATACC ATTCAGAAGG 
     3961 CTGACAAGAA GAGCATGGAA TGGTTTACTG TCATTGAGCA TTATCATCGA ACCAGTGCCA 
     4021 CCATTACTGA ATTGGTCATA GGGAATGAAT ATTACTTCCG GGTCTTTTCT GAAAACATGT 
     4081 GTGGCCTCAG TGAGGATGCC ACCATGACTA AAGAGAGTGC AGTGATCGCC AGGGATGGTA 
     4141 AAATCTACAA AAATCCAGTG TATGAAGACT TTGATTTCTC AGAGGCACCC ATGTTTACTC 
     4201 AGCCTTTGGT TAACACCTAT GCCATAGCTG GTTACAATGC CACCCTAAAC TGCAGTGTGA 
     4261 GAGGAAATCC TAAGCCTAAA ATAACCTGGA TGAAAAACAA AGTTGCTATT GTGGATGATC 
     4321 CAAGATACAG GATGTTCAGC AACCAGGGAG TCTGTACCCT GGAAATTCGC AAGCCCAGCC 
     4381 CCTATGATGG AGGCACTTAC TGCTGCAAAG CAGTCAATGA CCTTGGGACA GTGGAGATTG 
     4441 AATGCAAACT GGAGGTGAAA GTCATTGCAC AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4501 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4561 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4621 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4681 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4741 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4801 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4861 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4921 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4981 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5041 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5101 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5161 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5221 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5281 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5341 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5401 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5461 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5521 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5581 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5641 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5701 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5761 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5821 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5881 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5941 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6001 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6061 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6121 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6181 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6241 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6301 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6361 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6421 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6481 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6541 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6601 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6661 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6721 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6781 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6841 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6901 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6961 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7021 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7081 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7141 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7201 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7261 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7321 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7381 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7441 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7501 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7561 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7741 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7801 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7861 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7921 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7981 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8041 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8101 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8161 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8221 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8281 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//