LOCUS       RC214686                8388 bp    DNA     circular  SYN 
DEFINITION  NFASC (Myc-DDK-tagged)-Human neurofascin (NFASC), transcript variant 4
ACCESSION   NM_015090
VERSION     NM_015090.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4545..4636 
                     /label="Myc-DDK"  
     misc_feature    4666..5251 
                     /label="PolyA signal"  
     misc_feature    4723..4742 
                     /label="Restriction Enzyme"  
     misc_feature    5527..6199 
                     /label="ColE1"  
     misc_feature    6629..7423 
                     /label="Kan/Neo"  
     misc_feature    7458..7760 
                     /label="SV40 ori"  
     misc_feature    7846..8323 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4527..4533 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4527 
                     /label="NFASC"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGCAG CCACCGCCGC CCTGGGTCCA TGCAGCCTTC CTCCTCTGCC 
     1081 TCCTCAGTCT TGGCGGAGCC ATCGAAATTC CTATGGATCT GACGCAGCCG CCAACCATCA 
     1141 CCAAGCAGTC AGCGAAGGAT CACATCGTGG ACCCCCGTGA TAACATCCTG ATTGAGTGTG 
     1201 AAGCAAAAGG GAACCCTGCC CCCAGCTTCC ACTGGACACG AAACAGCAGA TTCTTCAACA 
     1261 TCGCCAAGGA CCCCCGGGTG TCCATGAGGA GGAGGTCTGG GACCCTGGTG ATTGACTTCC 
     1321 GCAGTGGCGG GCGGCCGGAG GAATATGAGG GGGAATATCA GTGCTTCGCC CGCAACAAAT 
     1381 TTGGCACGGC CCTGTCCAAT AGGATCCGCC TGCAGGTGTC TAAATCTCCT CTGTGGCCCA 
     1441 AGGAAAACCT AGACCCTGTC GTGGTCCAAG AGGGCGCTCC TTTGACGCTC CAGTGCAACC 
     1501 CCCCGCCTGG ACTTCCATCC CCGGTCATCT TCTGGATGAG CAGCTCCATG GAGCCCATCA 
     1561 CCCAAGACAA ACGTGTCTCT CAGGGCCATA ACGGAGACCT ATACTTCTCC AACGTGATGC 
     1621 TGCAGGACAT GCAGACCGAC TACAGTTGTA ACGCCCGCTT CCACTTCACC CACACCATCC 
     1681 AGCAGAAGAA CCCTTTCACC CTCAAGGTCC TCACCAACCA CCCTTATAAT GACTCGTCCT 
     1741 TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC AGAAAGAACA CCAAGCTTCA 
     1801 TGTATCCCCA GGGCACCGCG AGCAGCCAGA TGGTGCTTCG TGGCATGGAC CTCCTGCTGG 
     1861 AATGCATCGC CTCCGGGGTC CCAACACCAG ACATCGCATG GTACAAGAAA GGTGGGGACC 
     1921 TCCCATCTGA TAAGGCCAAG TTTGAGAACT TTAATAAGGC CCTGCGTATC ACAAATGTCT 
     1981 CTGAGGAAGA CTCCGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC AGCATCCGGC 
     2041 ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGACGAACCC AAGAACCTTA 
     2101 TTCTGGCTCC TGGCGAGGAT GGGAGACTGG TGTGTCGAGC CAATGGAAAC CCCAAACCCA 
     2161 CTGTCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCTAAC CCAAACCGTG 
     2221 AGGTGGCCGG AGACACCATC ATCTTCCGGG ACACCCAGAT CAGCAGCAGG GCTGTGTACC 
     2281 AGTGCAACAC CTCCAACGAG CATGGCTACC TGCTGGCCAA CGCCTTTGTC AGTGTGCTGG 
     2341 ATGTGCCGCC TCGGATGCTG TCGCCCCGGA ACCAGCTCAT TCGAGTGATT CTTTACAACC 
     2401 GGACGCGGCT GGACTGCCCT TTCTTTGGGT CTCCCATCCC CACACTGCGA TGGTTTAAGA 
     2461 ATGGGCAAGG AAGCAACCTG GATGGTGGCA ACTACCATGT TTATGAGAAC GGCAGTCTGG 
     2521 AAATTAAGAT GATCCGCAAA GAGGACCAGG GCATCTACAC CTGTGTCGCC ACCAACATCC 
     2581 TGGGCAAAGC TGAAAACCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG ATCTACCGGA 
     2641 TGCCCGAGGA CCAGGTGGCC AGAAGGGGCA CCACGGTGCA GCTGGAGTGT CGGGTGAAGC 
     2701 ACGACCCCTC CCTGAAACTC ACCGTCTCCT GGCTGAAGGA TGACGAGCCG CTCTATATTG 
     2761 GAAACAGGAT GAAGAAGGAA GACGACTCCC TGACCATCTT TGGGGTGGCA GAGCGGGACC 
     2821 AGGGCAGTTA CACGTGTGTC GCCAGCACCG AGCTAGACCA AGACCTGGCC AAGGCCTACC 
     2881 TCACCGTGCT AGGACGGCCA GACCGGCCCC GGGACCTGGA GCTGACCGAC CTGGCCGAGA 
     2941 GGAGCGTGCG GCTGACCTGG ATCCCCGGGG ATGCTAACAA CAGCCCCATC ACAGACTACG 
     3001 TCGTCCAGTT TGAAGAAGAC CAGTTCCAAC CTGGGGTCTG GCATGACCAT TCCAAGTACC 
     3061 CCGGCAGCGT TAACTCAGCC GTCCTCCGGC TGTCCCCGTA TGTCAACTAC CAGTTCCGTG 
     3121 TCATTGCCAT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT CCCATCCGAG CGCTACCGAA 
     3181 CCAGTGGAGC ACCCCCCGAG TCCAATCCTG GTGACGTGAA GGGAGAGGGG ACCAGAAAGA 
     3241 ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC GGCCTTTGGC CCCAACCTGC 
     3301 GCTACATTGT CAAGTGGAGG CGGAGAGAGA CTCGAGAGGC CTGGAACAAC GTCACAGTGT 
     3361 GGGGCTCTCG CTACGTGGTG GGGCAGACCC CAGTCTACGT GCCCTATGAG ATCCGAGTCC 
     3421 AGGCTGAAAA TGACTTCGGG AAGGGCCCTG AGCCAGAGTC CGTCATCGGT TACTCCGGAG 
     3481 AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAGTCCG AGTCATGAAC AGCACAGCCA 
     3541 TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCAGCTC AGAGAGTACC 
     3601 GAGCCTACTA CTGGAGGGAG AGCAGCTTGC TGAAGAACCT GTGGGTGTCT CAGAAGAGAC 
     3661 AGCAAGCCAG CTTCCCTGGT GACCGCCTCC GTGGCGTGGT GTCCCGCCTC TTCCCCTACA 
     3721 GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT CGCAGTGAGA 
     3781 CCAAGGAGTT CACCACCCCG GAAGGAGTAC CCAGTGCCCC TAGGCGTTTC CGAGTCCGGC 
     3841 AGCCCAACCT GGAGACAATC AACCTGGAAT GGGATCATCC TGAGCATCCA AATGGGATCA 
     3901 TGATTGGATA CACTCTCAAA TATGTGGCCT TTAACGGGAC CAAAGTAGGA AAGCAGATAG 
     3961 TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAACGGAC CCCGTGTCAC 
     4021 GCTACCGCTT TACCCTCAGC GCCAGGACGC AGGTGGGCTC TGGGGAAGCC GTCACAGAGG 
     4081 AGTCACCAGC ACCCCCGAAT GAAGCTTACA CCAACAACCA AGCGGACATC GCCACCCAGG 
     4141 GCTGGTTCAT TGGGCTTATG TGCGCCATCG CCCTCCTGGT GCTGATCCTG CTCATCGTCT 
     4201 GTTTCATCAA GAGGAGTCGC GGCGGCAAGT ACCCAGTACG AGAAAAGAAG GATGTTCCCC 
     4261 TTGGCCCTGA AGACCCCAAG GAAGAGGATG GCTCATTTGA CTATAGTGAT GAGGACAACA 
     4321 AGCCCCTGCA GGGCAGTCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG GAGAGTGACG 
     4381 ACAGCCTGGT GGACTATGGC GAGGGTGGCG AGGGTCAGTT CAATGAAGAC GGCTCCTTCA 
     4441 TCGGCCAGTA CACGGTCAAA AAGGACAAGG AGGAAACAGA GGGCAACGAA AGCTCAGAGG 
     4501 CCACGTCACC TGTCAATGCT ATCTACTCTC TGGCCACGCG TACGCGGCCG CTCGAGCAGA 
     4561 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4621 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4681 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4741 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4801 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4861 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4921 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4981 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5041 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5101 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5161 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5221 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5281 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5341 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5401 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5461 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5521 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5581 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5641 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5701 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5761 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5821 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5881 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5941 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6001 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6061 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6121 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6181 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6241 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6301 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6361 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6421 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6481 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6541 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6601 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6661 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6721 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6781 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6841 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6901 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6961 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7021 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7081 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7141 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7201 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7261 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7321 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7381 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7441 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7501 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7561 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7621 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7801 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7861 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7921 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7981 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8041 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8101 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8161 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8221 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8281 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8341 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//