LOCUS       RC214675                8394 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK-tagged)-Human myosin binding protein C, slow type (MYBPC1), transcript variant 1
ACCESSION   NM_002465
VERSION     NM_002465.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGAAAATGA AGTGCCAGCC CCAGCCCCAC 
     1081 CCCCGGAAGA ACCAAGTAAA GAGAAGGAGG CCGGAACTAC ACCAGCAAAA GATGAAGAGG 
     1141 AAGTCTCCCC GCCTAGCGCC TTGCCTCCAG GTTTGGGTAG TCGGGCCCTG GAGAGAAAAG 
     1201 ATTCAGACTG GACCCTTGTC GAAACTCCTC CTGGGGAGGA ACAAGCCAAG CAGAATGCCA 
     1261 ACTCCCAGCT GTCCATCTTG TTCATTGAAA AACCTCAAGG AGGAACAGTG AAAGTTGGTG 
     1321 AAGATATCAC CTTCATAGCC AAAGTCAAGG CTGAAGATCT TCTGAGAAAA CCCACTATCA 
     1381 AATGGTTCAA AGGAAAATGG ATGGACCTGG CCAGCAAAGC CGGGAAGCAC CTTCAGCTGA 
     1441 AGGAAACCTT TGAGAGGCAC AGTCGGGTGT ACACATTTGA GATGCAGATC ATCAAGGCCA 
     1501 AAGATAACTT TGCAGGAAAT TACAGATGCG AGGTCACCTA TAAGGATAAG TTTGACAGCT 
     1561 GTTCATTTGA TCTTGAAGTG CACGAATCTA CTGGGACTAC TCCAAACATT GACATCAGAT 
     1621 CTGCTTTCAA GAGAAGTGGA GAAGGTCAAG AGGATGCAGG AGAACTTGAC TTTAGTGGTC 
     1681 TCCTGAAACG TAGGGAGGTG AAGCAGCAGG AGGAAGAACC CCAGGTGGAC GTATGGGAGT 
     1741 TGCTGAAGAA CGCGAAACCC AGTGAGTACG AGAAGATCGC CTTCCAGTAT GGAATCACCG 
     1801 ACCTGCGCGG CATGCTCAAG CGACTCAAGC GCATGCGCAG AGAGGAGAAG AAGAGCGCAG 
     1861 CTTTTGCAAA AATTCTTGAT CCTGCATATC AGGTTGACAA AGGAGGCAGA GTGAGGTTTG 
     1921 TTGTGGAGCT GGCAGATCCA AAGTTGGAGG TGAAATGGTA TAAAAATGGT CAAGAAATTC 
     1981 GACCCAGTAC CAAATACATC TTTGAACACA AAGGATGCCA GAGAATCCTG TTTATCAATA 
     2041 ACTGTCAGAT GACAGATGAT TCAGAGTATT ATGTGACAGC CGGTGATGAG AAATGTTCCA 
     2101 CTGAGCTCTT CGTAAGAGAG CCTCCAATTA TGGTGACCAA ACAGCTGGAA GATACAACTG 
     2161 CTTATTGTGG GGAGAGAGTG GAATTAGAAT GTGAGGTGTC TGAAGATGAT GCCAATGTAA 
     2221 AATGGTTTAA GAATGGTGAA GAGATTATCC CTGGTCCAAA ATCAAGATAC CGAATTAGAG 
     2281 TTGAGGGTAA AAAACACATC TTGATCATAG AGGGAGCAAC AAAGGCTGAT GCTGCAGAAT 
     2341 ATTCAGTAAT GACAACAGGA GGACAATCAT CTGCTAAACT TAGTGTTGAC TTGAAACCTC 
     2401 TGAAGATTTT GACACCTCTG ACTGATCAGA CTGTAAATCT TGGAAAAGAA ATCTGCCTGA 
     2461 AGTGTGAAAT CTCTGAAAAC ATACCAGGAA AATGGACTAA AAATGGCCTA CCTGTTCAGG 
     2521 AGAGTGACCG TCTAAAGGTG GTTCACAAGG GAAGGATCCA CAAGTTAGTG ATAGCCAATG 
     2581 CCCTCACTGA AGATGAAGGT GATTATGTAT TTGCACCTGA TGCCTACAAT GTTACTCTGC 
     2641 CTGCCAAAGT TCATGTTATT GATCCTCCTA AGATCATCCT GGATGGTCTT GATGCTGACA 
     2701 ACACAGTGAC AGTGATTGCA GGAAACAAGC TTCGTCTTGA GATCCCCATC AGCGGAGAAC 
     2761 CACCTCCTAA AGCCATGTGG AGCCGGGGAG ATAAGGCTAT TATGGAAGGC AGTGGCCGGA 
     2821 TAAGAACAGA ATCTTACCCT GATAGCAGCA CTCTGGTCAT TGATATAGCT GAAAGAGATG 
     2881 ACTCTGGTGT TTACCACATC AATCTGAAAA ACGAAGCTGG AGAGGCACAT GCAAGCATCA 
     2941 AGGTTAAAGT TGTGGACTTC CCTGATCCTC CAGTGGCACC GACTGTGACA GAGGTGGGAG 
     3001 ATGACTGGTG TATCATGAAC TGGGAGCCTC CTGCCTACGA CGGAGGCTCT CCAATCCTAG 
     3061 GATATTTTAT TGAGAGGAAG AAGAAACAAA GCTCCAGGTG GATGAGGCTG AATTTTGATC 
     3121 TCTGCAAAGA AACAACTTTT GAGCCCAAGA AGATGATTGA AGGTGTGGCC TATGAGGTCC 
     3181 GCATCTTTGC AGTCAATGCC ATTGGCATCT CCAAGCCCAG TATGCCCTCC AGGCCTTTTG 
     3241 TTCCTTTGGC TGTAACAAGC CCTCCTACTC TTCTGACTGT GGACTCTGTC ACTGACACGA 
     3301 CTGTCACGAT GAGGTGGCGC CCCCCAGACC ACATTGGTGC AGCAGGTTTA GATGGCTATG 
     3361 TGCTAGAGTA TTGCTTTGAA GGAACTGAGG ACTGGATAGT TGCAAACAAA GATCTGATTG 
     3421 ACAAGACGAA GTTCACCATC ACAGGTCTGC CAACAGATGC AAAGATCTTT GTGCGTGTGA 
     3481 AGGCTGTTAA TGCAGCTGGT GCCAGCGAGC CCAAGTACTA TTCTCAGCCC ATTCTCGTGA 
     3541 AGGAAATCAT AGAACCTCCA AAGATTCGCA TTCCAAGACA CCTGAAGCAA ACCTATATCC 
     3601 GCAGAGTTGG AGAAGCTGTC AATCTGGTTA TACCTTTCCA GGGAAAACCA AGACCAGAAT 
     3661 TAACTTGGAA GAAGGATGGT GCAGAAATTG ATAAGAATCA AATAAACATT CGCAACTCTG 
     3721 AGACTGATAC AATCATATTT ATTAGAAAAG CAGAGAGGAG CCACTCTGGG AAATATGATC 
     3781 TGCAAGTCAA AGTGGACAAA TTCGTGGAGA CCGCATCAAT TGACATCCAG ATCATTGACC 
     3841 GTCCAGGTCC ACCCCAAATT GTGAAGATTG AGGATGTCTG GGGAGAAAAT GTCGCTCTCA 
     3901 CATGGACTCC ACCAAAGGAT GATGGAAATG CTGCTATCAC AGGCTATACC ATTCAGAAGG 
     3961 CTGACAAGAA GAGCATGGAA TGGTTTACTG TCATTGAGCA TTATCATCGA ACCAGTGCCA 
     4021 CCATTACTGA ATTGGTCATA GGGAATGAAT ATTACTTCCG GGTCTTTTCT GAAAACATGT 
     4081 GTGGCCTCAG TGAGGATGCC ACCATGACTA AAGAGAGTGC AGTGATCGCC AGGGATGGTA 
     4141 AAATCTACAA AAATCCAGTG TATGAAGACT TTGATTTCTC AGAGGCACCC ATGTTTACTC 
     4201 AGCCTTTGGT TAACACCTAT GCCATAGCTG GTTACAATGC CACCCTAAAC TGCAGTGTGA 
     4261 GAGGAAATCC TAAGCCTAAA ATAACCTGGA TGAAAAACAA AGTTGCTATT GTGGATGATC 
     4321 CAAGATACAG GATGTTCAGC AACCAGGGAG TCTGTACCCT GGAAATTCGC AAGCCCAGCC 
     4381 CCTATGATGG AGGCACTTAC TGCTGCAAAG CAGTCAATGA CCTTGGGACA GTGGAGATTG 
     4441 AATGCAAACT GGAGGTGAAA GTGATATATC AAGGAGTAAA TACCCCTGGA CAACCAGTCT 
     4501 TCCTGGAGGG GCAGCAACAG TCATTGCACA ATAAGGATTT TACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//