LOCUS       RC214622L2                9300 bp    DNA     circular  SYN 
DEFINITION  PPM1E (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E)
ACCESSION   NM_014906
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7591..7771 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8499..9158 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7819..8438 
                     /label="ColE1"  
     misc_feature    6466..6486 
                     /label="LR50"  
     misc_feature    5606..6331 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6475..7066 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5585..5591 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5585 
                     /label="PPM1E"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCGGCTGC ATCCCTGAGG AGAAAACTTA 
     3361 CCGGCGCTTC CTGGAGCTAT TCCTGGGCGA GTTTCGCGGA CCGTGCGGCG GCGGCGAGCC 
     3421 GGAGCCGGAA CCCGAACCCG AACCCGAACC CGAACCCGAG TCCGAGCCCG AGCCCGAACC 
     3481 TGAACTGGTA GAAGCTGAGG CGGCCGAGGC TTCGGTAGAG GAACCCGGGG AGGAGGCGGC 
     3541 CACGGTAGCC GCGACGGAGG AGGGGGACCA GGAGCAAGAC CCGGAGCCCG AGGAGGAGGC 
     3601 GGCGGTTGAG GGTGAGGAGG AGGAGGAGGG CGCGGCGACG GCGGCGGCAG CCCCGGGGCA 
     3661 CTCGGCCGTG CCGCCGCCGC CGCCCCAGCT GCCGCCTTTG CCCCCGCTCC CGCGACCGCT 
     3721 GTCAGAGCGC ATCACCCGCG AGGAGGTGGA GGGCGAAAGC CTGGACCTGT GCCTGCAGCA 
     3781 GCTCTACAAA TATAATTGCC CTTCCTTTTT GGCTGCTGCT TTAGCCAGAG CCACATCAGA 
     3841 TGAAGTCCTT CAGAGTGATC TTTCTGCACA TTATATCCCA AAGGAAACGG ATGGCACAGA 
     3901 AGGGACTGTG GAGATTGAGA CAGTGAAATT GGCCCGTTCT GTCTTCAGCA AACTACACGA 
     3961 GATTTGCTGC AGCTGGGTGA AAGACTTCCC CCTCCGCAGG AGACCCCAGC TTTATTATGA 
     4021 GACATCAATC CATGCCATCA AAAACATGCG CAGGAAAATG GAGGACAAAC ATGTCTGCAT 
     4081 TCCTGACTTT AATATGCTCT TCAACCTAGA GGACCAGGAA GAACAAGCTT ACTTTGCAGT 
     4141 GTTTGATGGC CATGGGGGAG TAGATGCTGC TATTTATGCC TCCATTCACC TCCACGTTAA 
     4201 CTTAGTCCGC CAGGAGATGT TCCCCCATGA TCCTGCTGAG GCCCTGTGCA GGGCCTTCCG 
     4261 GGTCACTGAT GAGCGGTTTG TGCAGAAAGC AGCCAGGGAG AGCTTAAGAT GTGGGACCAC 
     4321 AGGAGTGGTG ACTTTCATCA GAGGCAACAT GCTACATGTG GCCTGGGTGG GTGATTCCCA 
     4381 GGTTATGCTT GTGAGAAAGG GCCAAGCTGT TGAACTAATG AAGCCACACA AACCAGACAG 
     4441 AGAGGATGAA AAGCAGAGAA TTGAGGCCCT TGGAGGTTGC GTAGTCTGGT TTGGTGCCTG 
     4501 GAGGGTGAAT GGAAGTCTGT CGGTTTCCAG AGCTATTGGA GATGCTGAAC ATAAGCCATA 
     4561 TATCTGTGGG GATGCAGATT CTGCCTCCAC TGTTCTGGAT GGGACCGAAG ACTACCTCAT 
     4621 TCTGGCCTGT GATGGCTTCT ATGACACCGT GAACCCTGAT GAGGCAGTGA AAGTTGTGTC 
     4681 CGACCACCTG AAAGAGAATA ATGGAGACAG CAGCATGGTT GCCCACAAAT TAGTGGCATC 
     4741 AGCTCGTGAT GCTGGGTCAA GTGATAACAT CACGGTTATT GTGGTATTCC TGAGGGACAT 
     4801 GAACAAAGCT GTAAATGTTA GTGAGGAATC AGATTGGACA GAGAACTCTT TTCAAGGAGG 
     4861 GCAAGAAGAT GGTGGGGATG ATAAGGAGAA TCATGGAGAG TGCAAACGCC CTTGGCCTCA 
     4921 GCACCAGTGC TCAGCACCAG CCGACCTAGG CTATGATGGG CGTGTGGATT CATTCACTGA 
     4981 TAGAACTAGC CTGAGCCCAG GGTCCCAAAT CAACGTGCTG GAAGACCCAG GCTACCTAGA 
     5041 TCTCACACAA ATAGAAGCAA GCAAACCTCA CAGTGCCCAG TTTTTGCTAC CAGTTGAGAT 
     5101 GTTTGGTCCT GGTGCACCAA AGAAAGCAAA TCTTATTAAT GAGTTAATGA TGGAGAAAAA 
     5161 ATCAGTTCAG TCATCATTGC CTGAATGGAG TGGTGCTGGA GAGTTTCCCA CTGCTTTCAA 
     5221 TTTGGGTTCA ACAGGGGAGC AGATATACAG AATGCAGAGC TTGTCTCCTG TCTGTTCAGG 
     5281 GTTGGAAAAT GAACAGTTCA AATCCCCGGG AAACAGAGTT TCTAGATTGT CTCATTTACG 
     5341 CCACCACTAC TCAAAGAAGT GGCACAGATT CAGGTTTAAT CCAAAGTTTT ATTCATTTCT 
     5401 CTCTGCTCAA GAGCCTTCCC ACAAAATAGG CACTAGCCTG TCCTCACTTA CTGGAAGTGG 
     5461 GAAGAGAAAT AGGATAAGAA GTTCTCTGCC ATGGAGGCAA AATAGTTGGA AAGGGTACAG 
     5521 TGAAAACATG AGGAAGCTCA GAAAGACTCA TGATATTCCA TGCCCAGATC TTCCTTGGAG 
     5581 CTATAAAATA GAAACGCGTA CGCGGCCGCT CGAGATGAGC GGGGGCGAGG AGCTGTTCGC 
     5641 CGGCATCGTG CCCGTGCTGA TCGAGCTGGA CGGCGACGTG CACGGCCACA AGTTCAGCGT 
     5701 GCGCGGCGAG GGCGAGGGCG ACGCCGACTA CGGCAAGCTG GAGATCAAGT TCATCTGCAC 
     5761 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTGGTGACC ACCCTCTGCT ACGGCATCCA 
     5821 GTGCTTCGCC CGCTACCCCG AGCACATGAA GATGAACGAC TTCTTCAAGA GCGCCATGCC 
     5881 CGAGGGCTAC ATCCAGGAGC GCACCATCCA GTTCCAGGAC GACGGCAAGT ACAAGACCCG 
     5941 CGGCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCAAGGA 
     6001 CTTCAAGGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAGCTTCA ACAGCCACAA 
     6061 CGTGTACATC CGCCCCGACA AGGCCAACAA CGGCCTGGAG GCTAACTTCA AGACCCGCCA 
     6121 CAACATCGAG GGCGGCGGCG TGCAGCTGGC CGACCACTAC CAGACCAACG TGCCCCTGGG 
     6181 CGACGGCCCC GTGCTGATCC CCATCAACCA CTACCTGAGC ACTCAGACCA AGATCAGCAA 
     6241 GGACCGCAAC GAGGCCCGCG ACCACATGGT GCTCCTGGAG TCCTTCAGCG CCTGCTGCCA 
     6301 CACCCACGGC ATGGACGAGC TGTACAGGTC CGGACTCAGA TAAGTTTAAA CGGCCGGCCG 
     6361 CGGTCTGTAC AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC 
     6421 GAAGTTATAC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC 
     6481 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 
     6541 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 
     6601 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 
     6661 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 
     6721 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 
     6781 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 
     6841 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 
     6901 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 
     6961 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 
     7021 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC 
     7081 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC 
     7141 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT 
     7201 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 
     7261 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT 
     7321 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG 
     7381 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA 
     7441 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT 
     7501 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC 
     7561 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA 
     7621 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 
     7681 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 
     7741 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA 
     7801 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     7861 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     7921 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     7981 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     8041 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     8101 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     8161 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     8221 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     8281 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     8341 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     8401 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     8461 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC 
     8521 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 
     8581 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 
     8641 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 
     8701 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
     8761 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 
     8821 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 
     8881 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 
     8941 GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 
     9001 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
     9061 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 
     9121 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT 
     9181 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     9241 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//