LOCUS       RC214622                7146 bp    DNA     circular  SYN 
DEFINITION  PPM1E (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E)
ACCESSION   NM_014906
VERSION     NM_014906.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3303..3394 
                     /label="Myc-DDK"  
     misc_feature    3424..4009 
                     /label="PolyA signal"  
     misc_feature    3481..3500 
                     /label="Restriction Enzyme"  
     misc_feature    4285..4957 
                     /label="ColE1"  
     misc_feature    5387..6181 
                     /label="Kan/Neo"  
     misc_feature    6216..6518 
                     /label="SV40 ori"  
     misc_feature    6604..7081 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3285..3291 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3285 
                     /label="PPM1E"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGCTGC ATCCCTGAGG AGAAAACTTA CCGGCGCTTC CTGGAGCTAT 
     1081 TCCTGGGCGA GTTTCGCGGA CCGTGCGGCG GCGGCGAGCC GGAGCCGGAA CCCGAACCCG 
     1141 AACCCGAACC CGAACCCGAG TCCGAGCCCG AGCCCGAACC TGAACTGGTA GAAGCTGAGG 
     1201 CGGCCGAGGC TTCGGTAGAG GAACCCGGGG AGGAGGCGGC CACGGTAGCC GCGACGGAGG 
     1261 AGGGGGACCA GGAGCAAGAC CCGGAGCCCG AGGAGGAGGC GGCGGTTGAG GGTGAGGAGG 
     1321 AGGAGGAGGG CGCGGCGACG GCGGCGGCAG CCCCGGGGCA CTCGGCCGTG CCGCCGCCGC 
     1381 CGCCCCAGCT GCCGCCTTTG CCCCCGCTCC CGCGACCGCT GTCAGAGCGC ATCACCCGCG 
     1441 AGGAGGTGGA GGGCGAAAGC CTGGACCTGT GCCTGCAGCA GCTCTACAAA TATAATTGCC 
     1501 CTTCCTTTTT GGCTGCTGCT TTAGCCAGAG CCACATCAGA TGAAGTCCTT CAGAGTGATC 
     1561 TTTCTGCACA TTATATCCCA AAGGAAACGG ATGGCACAGA AGGGACTGTG GAGATTGAGA 
     1621 CAGTGAAATT GGCCCGTTCT GTCTTCAGCA AACTACACGA GATTTGCTGC AGCTGGGTGA 
     1681 AAGACTTCCC CCTCCGCAGG AGACCCCAGC TTTATTATGA GACATCAATC CATGCCATCA 
     1741 AAAACATGCG CAGGAAAATG GAGGACAAAC ATGTCTGCAT TCCTGACTTT AATATGCTCT 
     1801 TCAACCTAGA GGACCAGGAA GAACAAGCTT ACTTTGCAGT GTTTGATGGC CATGGGGGAG 
     1861 TAGATGCTGC TATTTATGCC TCCATTCACC TCCACGTTAA CTTAGTCCGC CAGGAGATGT 
     1921 TCCCCCATGA TCCTGCTGAG GCCCTGTGCA GGGCCTTCCG GGTCACTGAT GAGCGGTTTG 
     1981 TGCAGAAAGC AGCCAGGGAG AGCTTAAGAT GTGGGACCAC AGGAGTGGTG ACTTTCATCA 
     2041 GAGGCAACAT GCTACATGTG GCCTGGGTGG GTGATTCCCA GGTTATGCTT GTGAGAAAGG 
     2101 GCCAAGCTGT TGAACTAATG AAGCCACACA AACCAGACAG AGAGGATGAA AAGCAGAGAA 
     2161 TTGAGGCCCT TGGAGGTTGC GTAGTCTGGT TTGGTGCCTG GAGGGTGAAT GGAAGTCTGT 
     2221 CGGTTTCCAG AGCTATTGGA GATGCTGAAC ATAAGCCATA TATCTGTGGG GATGCAGATT 
     2281 CTGCCTCCAC TGTTCTGGAT GGGACCGAAG ACTACCTCAT TCTGGCCTGT GATGGCTTCT 
     2341 ATGACACCGT GAACCCTGAT GAGGCAGTGA AAGTTGTGTC CGACCACCTG AAAGAGAATA 
     2401 ATGGAGACAG CAGCATGGTT GCCCACAAAT TAGTGGCATC AGCTCGTGAT GCTGGGTCAA 
     2461 GTGATAACAT CACGGTTATT GTGGTATTCC TGAGGGACAT GAACAAAGCT GTAAATGTTA 
     2521 GTGAGGAATC AGATTGGACA GAGAACTCTT TTCAAGGAGG GCAAGAAGAT GGTGGGGATG 
     2581 ATAAGGAGAA TCATGGAGAG TGCAAACGCC CTTGGCCTCA GCACCAGTGC TCAGCACCAG 
     2641 CCGACCTAGG CTATGATGGG CGTGTGGATT CATTCACTGA TAGAACTAGC CTGAGCCCAG 
     2701 GGTCCCAAAT CAACGTGCTG GAAGACCCAG GCTACCTAGA TCTCACACAA ATAGAAGCAA 
     2761 GCAAACCTCA CAGTGCCCAG TTTTTGCTAC CAGTTGAGAT GTTTGGTCCT GGTGCACCAA 
     2821 AGAAAGCAAA TCTTATTAAT GAGTTAATGA TGGAGAAAAA ATCAGTTCAG TCATCATTGC 
     2881 CTGAATGGAG TGGTGCTGGA GAGTTTCCCA CTGCTTTCAA TTTGGGTTCA ACAGGGGAGC 
     2941 AGATATACAG AATGCAGAGC TTGTCTCCTG TCTGTTCAGG GTTGGAAAAT GAACAGTTCA 
     3001 AATCCCCGGG AAACAGAGTT TCTAGATTGT CTCATTTACG CCACCACTAC TCAAAGAAGT 
     3061 GGCACAGATT CAGGTTTAAT CCAAAGTTTT ATTCATTTCT CTCTGCTCAA GAGCCTTCCC 
     3121 ACAAAATAGG CACTAGCCTG TCCTCACTTA CTGGAAGTGG GAAGAGAAAT AGGATAAGAA 
     3181 GTTCTCTGCC ATGGAGGCAA AATAGTTGGA AAGGGTACAG TGAAAACATG AGGAAGCTCA 
     3241 GAAAGACTCA TGATATTCCA TGCCCAGATC TTCCTTGGAG CTATAAAATA GAAACGCGTA 
     3301 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3361 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3421 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3481 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3541 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3601 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3661 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3721 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3781 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3841 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3901 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3961 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4021 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4081 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4141 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4201 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4261 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4321 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4381 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4441 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4501 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4561 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4621 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4681 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4741 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4801 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4861 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4921 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4981 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5041 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5101 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5161 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5221 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5281 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5341 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5401 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5461 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5521 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5581 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5641 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5701 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5761 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5821 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5881 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5941 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6001 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6061 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6121 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6181 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6241 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6301 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6361 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6421 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6481 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6541 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6601 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6661 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6721 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6781 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6841 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6901 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6961 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7021 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7081 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7141 AATTTT
//