LOCUS       RC214507                8538 bp    DNA     circular  SYN 
DEFINITION  RTEL1 (Myc-DDK-tagged)-Human regulator of telomere elongation helicase 1 (RTEL1), transcript variant 1
ACCESSION   NM_016434
VERSION     NM_016434.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="RTEL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCAAGATA GTCCTGAATG GTGTGACCGT AGACTTCCCT TTCCAGCCCT 
     1081 ACAAATGCCA ACAGGAGTAC ATGACCAAGG TCCTGGAATG TCTGCAGCAG AAGGTGAATG 
     1141 GCATCCTGGA GAGCCCTACG GGTACAGGGA AGACGCTGTG CCTGCTGTGC ACCACGCTGG 
     1201 CCTGGCGAGA ACACCTCCGA GACGGCATCT CTGCCCGCAA GATTGCCGAG AGGGCGCAAG 
     1261 GAGAGCTTTT CCCGGATCGG GCCTTGTCAT CCTGGGGCAA CGCTGCTGCT GCTGCTGGAG 
     1321 ACCCCATAGC TTGCTACACG GACATCCCAA AGATTATTTA CGCCTCCAGG ACCCACTCGC 
     1381 AACTCACACA GGTCATCAAC GAGCTTCGGA ACACCTCCTA CCGGCCTAAG GTGTGTGTGC 
     1441 TGGGCTCCCG GGAGCAGCTG TGCATCCATC CTGAGGTGAA GAAACAAGAG AGTAACCATC 
     1501 TACAGATCCA CTTGTGCCGT AAGAAGGTGG CAAGTCGCTC CTGTCATTTC TACAACAACG 
     1561 TAGAAGAAAA AAGCCTGGAG CAGGAGCTGG CCAGCCCCAT CCTGGACATT GAGGACTTGG 
     1621 TCAAGAGCGG AAGCAAGCAC AGGGTGTGCC CTTACTACCT GTCCCGGAAC CTGAAGCAGC 
     1681 AAGCCGACAT CATATTCATG CCGTACAATT ACTTGTTGGA TGCCAAGAGC CGCAGAGCAC 
     1741 ACAACATTGA CCTGAAGGGG ACAGTCGTGA TCTTTGACGA AGCTCACAAC GTGGAGAAGA 
     1801 TGTGTGAAGA ATCGGCATCC TTTGACCTGA CTCCCCATGA CCTGGCTTCA GGACTGGACG 
     1861 TCATAGACCA GGTGCTGGAG GAGCAGACCA AGGCAGCGCA GCAGGGTGAG CCCCACCCGG 
     1921 AGTTCAGCGC GGACTCCCCC AGCCCAGGGC TGAACATGGA GCTGGAAGAC ATTGCAAAGC 
     1981 TGAAGATGAT CCTGCTGCGC CTGGAGGGGG CCATCGATGC TGTTGAGCTG CCTGGAGACG 
     2041 ACAGCGGTGT CACCAAGCCA GGGAGCTACA TCTTTGAGCT GTTTGCTGAA GCCCAGATCA 
     2101 CGTTTCAGAC CAAGGGCTGC ATCCTGGACT CGCTGGACCA GATCATCCAG CACCTGGCAG 
     2161 GACGTGCTGG AGTGTTCACC AACACGGCCG GACTGCAGAA GCTGGCGGAC ATTATCCAGA 
     2221 TTGTGTTCAG TGTGGACCCC TCCGAGGGCA GCCCTGGTTC CCCAGCAGGG CTGGGGGCCT 
     2281 TACAGTCCTA TAAGGTGCAC ATCCATCCTG ATGCTGGTCA CCGGAGGACG GCTCAGCGGT 
     2341 CTGATGCCTG GAGCACCACT GCAGCCAGAA AGCGAGGGAA GGTGCTGAGC TACTGGTGCT 
     2401 TCAGTCCCGG CCACAGCATG CACGAGCTGG TCCGCCAGGG CGTCCGCTCC CTCATCCTTA 
     2461 CCAGCGGCAC GCTGGCCCCG GTGTCCTCCT TTGCTCTGGA GATGCAGATC CCTTTCCCAG 
     2521 TCTGCCTGGA GAACCCACAC ATCATCGACA AGCACCAGAT CTGGGTGGGG GTCGTCCCCA 
     2581 GAGGCCCCGA TGGAGCCCAG TTGAGCTCCG CGTTTGACAG ACGGTTTTCC GAGGAGTGCT 
     2641 TATCCTCCCT GGGGAAGGCT CTGGGCAACA TCGCCCGCGT GGTGCCCTAT GGGCTCCTGA 
     2701 TCTTCTTCCC TTCCTATCCT GTCATGGAGA AGAGCCTGGA GTTCTGGCGG GCCCGCGACT 
     2761 TGGCCAGGAA GATGGAGGCG CTGAAGCCGC TGTTTGTGGA GCCCAGGAGC AAAGGCAGCT 
     2821 TCTCCGAGAC CATCAGTGCT TACTATGCAA GGGTTGCCGC CCCTGGGTCC ACCGGCGCCA 
     2881 CCTTCCTGGC GGTCTGCCGG GGCAAGGCCA GCGAGGGGCT GGACTTCTCA GACACGAATG 
     2941 GCCGTGGTGT GATTGTCACG GGCCTCCCGT ACCCCCCACG CATGGACCCC CGGGTTGTCC 
     3001 TCAAGATGCA GTTCCTGGAT GAGATGAAGG GCCAGGGTGG GGCTGGGGGC CAGTTCCTCT 
     3061 CTGGGCAGGA GTGGTACCGG CAGCAGGCGT CCAGGGCTGT GAACCAGGCC ATCGGGCGAG 
     3121 TGATCCGGCA CCGCCAGGAC TACGGAGCTG TCTTCCTCTG TGACCACAGG TTCGCCTTTG 
     3181 CCGACGCAAG AGCCCAACTG CCCTCCTGGG TGCGTCCCCA CGTCAGGGTG TATGACAACT 
     3241 TTGGCCATGT CATCCGAGAC GTGGCCCAGT TCTTCCGTGT TGCCGAGCGA ACTATGCCAG 
     3301 CGCCGGCCCC CCGGGCTACA GCACCCAGTG TGCGTGGAGA AGATGCTGTC AGCGAGGCCA 
     3361 AGTCGCCTGG CCCCTTCTTC TCCACCAGGA AAGCTAAGAG TCTGGACCTG CATGTCCCCA 
     3421 GCCTGAAGCA GAGGTCCTCA GGGTCACCAG CTGCCGGGGA CCCCGAGAGT AGCCTGTGTG 
     3481 TGGAGTATGA GCAGGAGCCA GTTCCTGCCC GGCAGAGGCC CAGGGGGCTG CTGGCCGCCC 
     3541 TGGAGCACAG CGAACAGCGG GCGGGGAGCC CTGGCGAGGA GCAGGCCCAC AGCTGCTCCA 
     3601 CCCTGTCCCT CCTGTCTGAG AAGAGGCCGG CAGAAGAACC GCGAGGAGGG AGGAAGAAGA 
     3661 TCCGGCTGGT CAGCCACCCG GAGGAGCCCG TGGCTGGTGC ACAGACGGAC AGGGCCAAGC 
     3721 TCTTCATGGT GGCCGTGAAG CAGGAGTTGA GCCAAGCCAA CTTTGCCACC TTCACCCAGG 
     3781 CCCTGCAGGA CTACAAGGGT TCCGATGACT TCGCCGCCCT GGCCGCCTGT CTCGGCCCCC 
     3841 TCTTTGCTGA GGACCCCAAG AAGCACAACC TGCTCCAAGG CTTCTACCAG TTTGTGCGGC 
     3901 CCCACCATAA GCAGCAGTTT GAGGAGGTCT GTATCCAGCT GACAGGACGA GGCTGTGGCT 
     3961 ATCGGCCTGA GCACAGCATT CCCCGAAGGC AGCGGGCACA GCCGGTCCTG GACCCCACTG 
     4021 GAAGAACGGC GCCGGATCCC AAGCTGACCG TGTCCACGGC TGCAGCCCAG CAGCTGGACC 
     4081 CCCAAGAGCA CCTGAACCAG GGCAGGCCCC ACCTGTCGCC CAGGCCACCC CCAACAGGAG 
     4141 ACCCTGGCAG CCAACCACAG TGGGGGTCTG GAGTGCCCAG AGCAGGGAAG CAGGGCCAGC 
     4201 ACGCCGTGAG CGCCTACCTG GCTGATGCCC GCAGGGCCCT GGGGTCCGCG GGCTGTAGCC 
     4261 AACTCTTGGC AGCGCTGACA GCCTATAAGC AAGACGACGA CCTCGACAAG GTGCTGGCTG 
     4321 TGTTGGCCGC CCTGACCACT GCAAAGCCAG AGGACTTCCC CCTGCTGCAC AGGTTCAGCA 
     4381 TGTTTGTGCG TCCACACCAC AAGCAGCGCT TCTCACAGAC GTGCACAGAC CTGACCGGCC 
     4441 GGCCCTACCC GGGCATGGAG CCACCGGGAC CCCAGGAGGA GAGGCTTGCC GTGCCTCCTG 
     4501 TGCTTACCCA CAGGGCTCCC CAACCAGGCC CCTCACGGTC CGAGAAGACC GGGAAGACCC 
     4561 AGAGCAAGAT CTCGTCCTTC CTTAGACAGA GGCCAGCAGG GACTGTGGGG GCGGGCGGTG 
     4621 AGGATGCAGG TCCCAGCCAG TCCTCAGGAC CTCCCCACGG GCCTGCAGCA TCTGAGTGGG 
     4681 GCCTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//