LOCUS       RC214488L1                9243 bp    DNA     circular  SYN 
DEFINITION  GRIK4 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 4 (GRIK4)
ACCESSION   NM_014619
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7534..7714 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8442..9101 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7873..8441 
                     /label="ColE1"  
     misc_feature    6409..6429 
                     /label="LR50 primer"  
     misc_feature    6206..6277 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6418..7009 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6188..6194 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6188 
                     /label="GRIK4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCCCGCGTC TCGGCGCCTT TGGTGCTGCT 
     3361 TCCTGCGTGG CTCGTGATGG TCGCCTGCAG CCCGCACTCC TTGAGGATCG CTGCTATCTT 
     3421 GGACGACCCC ATGGAGTGCA GCAGAGGGGA GCGGCTCTCC ATCACCCTGG CCAAGAACCG 
     3481 CATCAACCGC GCTCCTGAGA GGCTGGGCAA GGCCAAGGTC GAAGTGGACA TCTTTGAGCT 
     3541 TCTCAGAGAC AGCGAGTACG AGACTGCAGA AACCATGTGT CAGATCCTCC CCAAGGGGGT 
     3601 GGTCGCTGTC CTCGGACCAT CGTCCAGCCC AGCCTCCAGC TCCATCATCA GCAACATCTG 
     3661 TGGAGAGAAG GAGGTCCCTC ACTTCAAAGT GGCCCCAGAG GAGTTCGTCA AGTTCCAGTT 
     3721 CCAGAGATTC ACAACCCTGA ACCTCCACCC CAGCAACACT GACATCAGCG TGGCTGTAGC 
     3781 TGGGATCCTG AACTTCTTCA ACTGCACCAC CGCCTGCCTC ATCTGTGCCA AAGCAGAATG 
     3841 CCTTTTAAAC CTAGAGAAGC TGCTCCGGCA ATTCCTTATC TCCAAGGACA CGCTGTCCGT 
     3901 CCGCATGCTG GATGACACCC GGGACCCCAC CCCGCTCCTC AAGGAGATCC GGGACGACAA 
     3961 GACCGCCACC ATCATCATCC ACGCCAACGC CTCCATGTCC CACACCATCC TCCTGAAGGC 
     4021 AGCCGAACTT GGGATGGTGT CAGCCTATTA CACATACATC TTCACTAATC TGGAGTTCTC 
     4081 ACTCCAGAGA ATGGACAGCC TTGTGGATGA TCGTGTCAAC ATCCTGGGAT TTTCCATTTT 
     4141 CAACCAATCC CATGCTTTCT TCCAAGAGTT TGCCCAGAGC CTCAACCAGT CCTGGCAGGA 
     4201 GAACTGTGAC CATGTGCCCT TCACTGGGCC TGCGCTCTCC TCGGCCCTGC TGTTTGATGC 
     4261 TGTCTATGCT GTGGTGACTG CGGTGCAGGA ACTGAACCGG AGCCAAGAGA TCGGCGTGAA 
     4321 GCCCTTGTCC TGCGGCTCGG CCCAGATCTG GCAGCACGGC ACCAGCCTCA TGAACTACCT 
     4381 GCGCATGGTA GAATTGGAAG GTCTTACCGG CCACATTGAA TTCAACAGCA AAGGCCAGAG 
     4441 GTCCAACTAC GCTTTGAAAA TCTTACAGTT CACAAGGAAT GGTTTTCGGC AGATCGGCCA 
     4501 GTGGCACGTG GCAGAGGGCC TCAGCATGGA CAGCCACCTC TATGCCTCCA ACATCTCGGA 
     4561 CACTCTCTTC AACACCACCC TGGTCGTCAC CACCATCCTG GAAAACCCAT ATTTAATGCT 
     4621 GAAGGGGAAC CACCAGGAGA TGGAAGGCAA TGACCGCTAC GAGGGCTTCT GTGTGGACAT 
     4681 GCTCAAGGAG CTGGCAGAGA TCCTCCGATT CAACTACAAG ATCCGCCTGG TTGGGGATGG 
     4741 CGTGTACGGC GTTCCCGAGG CCAACGGCAC CTGGACGGGA ATGGTCGGGG AGCTGATCGC 
     4801 TAGGAAAGCA GATCTGGCTG TGGCAGGCCT CACCATTACA GCTGAACGGG AGAAGGTGAT 
     4861 TGATTTCTCT AAGCCATTCA TGACTCTGGG AATTAGCATT CTTTACCGCG TTCATATGGG 
     4921 ACGCAAACCC GGCTATTTCT CCTTCCTGGA CCCATTTTCT CCGGGCGTCT GGCTCTTCAT 
     4981 GCTTCTAGCC TATCTGGCCG TCAGCTGTGT CCTCTTCCTG GTGGCTCGGT TGACGCCCTA 
     5041 CGAGTGGTAC AGCCCACACC CATGTGCCCA GGGCCGGTGC AACCTCCTGG TGAACCAGTA 
     5101 CTCCCTGGGC AACAGCCTCT GGTTTCCGGT CGGGGGGTTC ATGCAGCAAG GCTCCACCAT 
     5161 CGCCCCTCGC GCCTTATCCA CCCGCTGTGT CAGTGGCGTC TGGTGGGCAT TCACGCTGAT 
     5221 CATCATCTCA TCCTACACGG CCAACCTGGC AGCCTTCCTG ACCGTGCAGC GCATGGATGT 
     5281 GCCCATTGAG TCAGTGGATG ACCTGGCTGA CCAGACCGCC ATTGAATATG GCACAATTCA 
     5341 CGGAGGCTCC AGCATGACCT TCTTCCAAAA TTCCCGCTAC CAGACCTACC AACGCATGTG 
     5401 GAATTACATG TATTCCAAGC AGCCCAGCGT GTTCGTGAAG AGCACAGAGG AGGGAATCGC 
     5461 CAGGGTGTTG AATTCCAACT ACGCCTTCCT CCTGGAATCC ACCATGAACG AGTACTATCG 
     5521 GCAGCGAAAC TGCAACCTCA CTCAGATTGG GGGCCTGCTG GACACCAAGG GCTATGGGAT 
     5581 TGGCATGCCA GTCGGCTCGG TTTTCCGGGA CGAGTTTGAT CTGGCCATTC TCCAGCTGCA 
     5641 GGAGAACAAC CGCCTGGAGA TCCTGAAGCG CAAATGGTGG GAAGGAGGGA AGTGCCCCAA 
     5701 GGAGGAAGAT CACAGAGCTA AAGGCCTGGG AATGGAGAAT ATTGGTGGAA TCTTTGTGGT 
     5761 TCTTATTTGT GGCTTAATCG TGGCCATTTT TATGGCTATG TTGGAGTTTT TATGGACTCT 
     5821 CAGACACTCA GAAGCAACTG AGGTGTCCGT CTGCCAGGAG ATGGTGACCG AGCTGCGCAG 
     5881 CATTATCCTG TGTCAGGACA GTATCCACCC CCGCCGGCGG CGCGCCGCAG TCCCGCCGCC 
     5941 CCGGCGCCCC ATCCCCGAGG AGCGCCGACC GCGGGGCACG GCGACGCTCA GCAACGGGAA 
     6001 GCTGTGCGGG GCAGGGGAGC CCGACCAGCT CGCGCAGAGA CTGGCGCAGG AGGCCGCCCT 
     6061 GGTGGCCCGC GGCTGCACGC ACATCCGCGT CTGCCCCGAG TGCCGCCGCT TCCAGGGCCT 
     6121 GCGGGCACGG CCGTCGCCCG CCCGCAGCGA GGAGAGCCTG GAGTGGGAGA AAACCACCAA 
     6181 CAGCAGCGAG CCCGAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     6241 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     6301 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     6361 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     6421 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6481 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6541 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6601 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6661 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6721 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     6781 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     6841 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     6901 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     6961 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     7021 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     7081 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     7141 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     7201 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     7261 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     7321 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     7381 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     7441 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7501 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7561 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7621 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7681 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7741 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     7801 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     7861 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     7921 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     7981 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     8041 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     8101 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     8161 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     8221 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     8281 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     8341 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     8401 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     8461 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8521 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8581 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8641 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8701 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     8761 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     8821 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     8881 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     8941 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     9001 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     9061 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     9121 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     9181 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     9241 GAC
//