LOCUS       RC214483L1                9531 bp    DNA     circular  SYN 
DEFINITION  UBA6 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6)
ACCESSION   NM_018227
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7822..8002 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8730..9389 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8161..8729 
                     /label="ColE1"  
     misc_feature    6697..6717 
                     /label="LR50 primer"  
     misc_feature    6494..6565 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6706..7297 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6476..6482 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6476 
                     /label="UBA6"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAAGGATCC GAGCCTGTGG CCGCCCATCA 
     3361 GGGGGAAGAG GCGTCCTGTT CTTCCTGGGG GACTGGCAGC ACAAATAAAA ATTTGCCCAT 
     3421 TATGTCAACA GCATCTGTGG AAATCGATGA TGCATTGTAT AGTCGACAGA GGTACGTTCT 
     3481 TGGAGACACA GCAATGCAGA AGATGGCCAA GTCCCATGTT TTCTTAAGTG GGATGGGTGG 
     3541 TCTTGGTTTG GAAATTGCAA AGAATCTTGT TCTTGCAGGG ATTAAGGCAG TTACAATTCA 
     3601 TGATACAGAA AAATGCCAAG CATGGGATCT AGGAACCAAC TTCTTTCTCA GTGAAGATGA 
     3661 TGTTGTTAAT AAGAGAAACA GGGCTGAAGC TGTACTTAAA CATATTGCAG AACTAAATCC 
     3721 ATACGTTCAT GTCACATCAT CTTCTGTTCC TTTCAATGAG ACCACAGATC TCTCCTTTTT 
     3781 AGATAAATAC CAGTGTGTAG TATTGACTGA GATGAAACTT CCATTGCAGA AGAAGATCAA 
     3841 TGACTTTTGC CGTTCTCAGT GCCCTCCAAT TAAGTTTATC AGTGCAGATG TACATGGAAT 
     3901 TTGGTCAAGG TTATTTTGTG ATTTCGGTGA TGAATTTGAA GTTTTAGATA CAACAGGAGA 
     3961 AGAACCAAAA GAAATTTTCA TTTCAAACAT AACGCAAGCA AATCCTGGCA TTGTTACTTG 
     4021 CCTTGAAAAT CATCCTCACA AACTGGAGAC AGGACAATTC CTAACATTTC GAGAAATTAA 
     4081 TGGAATGACA GGTTTAAATG GATCTATACA ACAAATAACG GTGATATCGC CATTTTCTTT 
     4141 TAGTATTGGT GACACCACAG AACTGGAACC ATATTTACAT GGAGGCATAG CTGTCCAAGT 
     4201 TAAGACTCCT AAAACAGTTT TTTTTGAATC ACTGGAGAGG CAGTTAAAAC ATCCAAAGTG 
     4261 CCTTATTGTG GATTTTAGCA ACCCTGAGGC ACCTTTAGAG ATTCACACAG CTATGCTTGC 
     4321 CTTGGACCAG TTTCAGGAGA AATACAGTCG CAAGCCAAAT GTTGGATGCC AACAAGATTC 
     4381 AGAAGAACTG TTGAAACTAG CAACATCTAT AAGTGAAACC TTGGAAGAGA AGCCTGATGT 
     4441 AAATGCTGAC ATTGTGCATT GGCTCTCTTG GACTGCCCAA GGCTTTTTAT CTCCACTTGC 
     4501 TGCAGCAGTA GGAGGTGTTG CCAGCCAAGA AGTATTGAAA GCTGTAACAG GAAAATTTTC 
     4561 TCCTTTGTGC CAGTGGTTAT ATCTTGAAGC AGCAGATATT GTTGAATCAC TAGGCAAACC 
     4621 TGAATGTGAA GAATTTCTCC CACGAGGAGA TAGATATGAT GCCTTAAGAG CTTGCATTGG 
     4681 AGACACTTTG TGTCAGAAAC TGCAAAATTT AAACATCTTC TTAGTAGGGT GTGGAGCCAT 
     4741 AGGCTGTGAA ATGTTGAAAA ATTTTGCTTT ACTTGGTGTT GGCACAAGCA AAGAGAAAGG 
     4801 AATGATTACA GTTACAGATC CTGACTTGAT AGAGAAATCC AACTTAAATA GACAGTTCCT 
     4861 ATTTCGTCCT CATCACATAC AGAAACCTAA AAGCTACACT GCTGCTGATG CTACTCTGAA 
     4921 AATAAATTCT CAAATAAAGA TAGATGCACA CCTGAACAAA GTATGTCCAA CCACTGAGAC 
     4981 CATTTACAAT GATGAGTTCT ATACTAAACA AGATGTAATT ATTACAGCAT TAGATAATGT 
     5041 GGAAGCCAGG AGATACGTAG ACAGTCGTTG CTTAGCAAAT CTAAGGCCTC TTTTAGATTC 
     5101 TGGAACAATG GGCACTAAGG GACACACTGA AGTTATTGTA CCGCATTTGA CTGAGTCTTA 
     5161 CAATAGTCAT CGGGATCCCC CAGAAGAGGA AATACCATTT TGTACTCTAA AATCCTTTCC 
     5221 AGCTGCTATT GAACATACCA TACAGTGGGC AAGAGATAAG TTTGAAAGTT CCTTTTCCCA 
     5281 CAAACCTTCA TTGTTTAACA AATTTTGGCA AACCTATTCA TCTGCAGAAG AAGTCTTACA 
     5341 GAAGATACAG AGTGGACACA GTTTAGAAGG CTGTTTTCAA GTTATAAAGT TACTTAGCAG 
     5401 AAGACCTAGA AATTGGTCCC AGTGTGTAGA ATTAGCAAGA TTAAAGTTTG AAAAATATTT 
     5461 TAACCATAAG GCTCTTCAGC TTCTTCACTG TTTCCCTCTG GACATACGAT TAAAAGATGG 
     5521 CAGTTTATTT TGGCAGTCAC CAAAGAGGCC ACCCTCTCCA ATAAAATTTG ATTTAAATGA 
     5581 GCCTTTGCAC CTCAGTTTCC TTCAGAATGC TGCAAAACTA TATGCTACAG TATATTGTAT 
     5641 TCCATTTGCA GAAGAGGACT TATCAGCAGA TGCCCTCTTG AATATTCTTT CAGAAGTAAA 
     5701 GATTCAGGAA TTCAAGCCTT CCAATAAGGT TGTTCAAACA GATGAAACTG CAAGGAAACC 
     5761 AGACCATGTT CCTATTAGCA GTGAAGATGA GAGGAATGCA ATTTTCCAAC TAGAAAAGGC 
     5821 TATTTTATCT AATGAAGCCA CCAAAAGTGA CCTTCAGATG GCAGTGCTTT CATTTGAAAA 
     5881 AGATGATGAT CATAATGGAC ACATAGATTT CATCACAGCT GCATCAAATC TTCGTGCCAA 
     5941 AATGTACAGC ATTGAACCAG CTGACCGTTT CAAAACAAAG CGCATAGCTG GTAAAATTAT 
     6001 ACCTGCTATA GCAACAACCA CTGCTACAGT TTCTGGCTTG GTTGCCTTGG AGATGATCAA 
     6061 AGTAACTGGT GGCTATCCAT TTGAAGCTTA CAAAAATTGT TTTCTTAACT TAGCCATTCC 
     6121 AATTGTAGTA TTTACAGAGA CAACTGAAGT AAGGAAAACT AAAATCAGAA ATGGAATATC 
     6181 ATTTACAATT TGGGATCGAT GGACCGTACA TGGAAAAGAA GATTTCACCC TCTTGGATTT 
     6241 CATAAATGCA GTCAAAGAGA AGTATGGAAT TGAGCCAACA ATGGTGGTAC AGGGAGTCAA 
     6301 AATGCTTTAT GTTCCTGTAA TGCCTGGTCA TGCAAAAAGA TTGAAGTTAA CAATGCATAA 
     6361 ACTTGTAAAA CCTACTACTG AAAAGAAATA TGTGGATCTT ACTGTGTCAT TTGCTCCAGA 
     6421 CATTGATGGA GATGAAGATT TGCCGGGACC TCCAGTAAGA TACTACTTCA GTCATGACAC 
     6481 TGATACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     6541 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     6601 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     6661 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     6721 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     6781 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     6841 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     6901 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6961 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     7021 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     7081 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     7141 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     7201 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     7261 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     7321 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     7381 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     7441 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     7501 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     7561 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     7621 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     7681 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     7741 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     7801 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     7861 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     7921 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7981 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     8041 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     8101 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     8161 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     8221 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     8281 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     8341 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     8401 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     8461 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     8521 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     8581 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     8641 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     8701 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     8761 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     8821 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     8881 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     8941 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     9001 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     9061 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     9121 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     9181 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     9241 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     9301 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     9361 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     9421 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     9481 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//