LOCUS       RC214483                8037 bp    DNA     circular  SYN 
DEFINITION  UBA6 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6)
ACCESSION   NM_018227
VERSION     NM_018227.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4194..4285 
                     /label="Myc-DDK"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    4372..4391 
                     /label="Restriction Enzyme"  
     misc_feature    5176..5848 
                     /label="ColE1"  
     misc_feature    6278..7072 
                     /label="Kan/Neo"  
     misc_feature    7107..7409 
                     /label="SV40 ori"  
     misc_feature    7495..7972 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4176..4182 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4176 
                     /label="UBA6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGGATCC GAGCCTGTGG CCGCCCATCA GGGGGAAGAG GCGTCCTGTT 
     1081 CTTCCTGGGG GACTGGCAGC ACAAATAAAA ATTTGCCCAT TATGTCAACA GCATCTGTGG 
     1141 AAATCGATGA TGCATTGTAT AGTCGACAGA GGTACGTTCT TGGAGACACA GCAATGCAGA 
     1201 AGATGGCCAA GTCCCATGTT TTCTTAAGTG GGATGGGTGG TCTTGGTTTG GAAATTGCAA 
     1261 AGAATCTTGT TCTTGCAGGG ATTAAGGCAG TTACAATTCA TGATACAGAA AAATGCCAAG 
     1321 CATGGGATCT AGGAACCAAC TTCTTTCTCA GTGAAGATGA TGTTGTTAAT AAGAGAAACA 
     1381 GGGCTGAAGC TGTACTTAAA CATATTGCAG AACTAAATCC ATACGTTCAT GTCACATCAT 
     1441 CTTCTGTTCC TTTCAATGAG ACCACAGATC TCTCCTTTTT AGATAAATAC CAGTGTGTAG 
     1501 TATTGACTGA GATGAAACTT CCATTGCAGA AGAAGATCAA TGACTTTTGC CGTTCTCAGT 
     1561 GCCCTCCAAT TAAGTTTATC AGTGCAGATG TACATGGAAT TTGGTCAAGG TTATTTTGTG 
     1621 ATTTCGGTGA TGAATTTGAA GTTTTAGATA CAACAGGAGA AGAACCAAAA GAAATTTTCA 
     1681 TTTCAAACAT AACGCAAGCA AATCCTGGCA TTGTTACTTG CCTTGAAAAT CATCCTCACA 
     1741 AACTGGAGAC AGGACAATTC CTAACATTTC GAGAAATTAA TGGAATGACA GGTTTAAATG 
     1801 GATCTATACA ACAAATAACG GTGATATCGC CATTTTCTTT TAGTATTGGT GACACCACAG 
     1861 AACTGGAACC ATATTTACAT GGAGGCATAG CTGTCCAAGT TAAGACTCCT AAAACAGTTT 
     1921 TTTTTGAATC ACTGGAGAGG CAGTTAAAAC ATCCAAAGTG CCTTATTGTG GATTTTAGCA 
     1981 ACCCTGAGGC ACCTTTAGAG ATTCACACAG CTATGCTTGC CTTGGACCAG TTTCAGGAGA 
     2041 AATACAGTCG CAAGCCAAAT GTTGGATGCC AACAAGATTC AGAAGAACTG TTGAAACTAG 
     2101 CAACATCTAT AAGTGAAACC TTGGAAGAGA AGCCTGATGT AAATGCTGAC ATTGTGCATT 
     2161 GGCTCTCTTG GACTGCCCAA GGCTTTTTAT CTCCACTTGC TGCAGCAGTA GGAGGTGTTG 
     2221 CCAGCCAAGA AGTATTGAAA GCTGTAACAG GAAAATTTTC TCCTTTGTGC CAGTGGTTAT 
     2281 ATCTTGAAGC AGCAGATATT GTTGAATCAC TAGGCAAACC TGAATGTGAA GAATTTCTCC 
     2341 CACGAGGAGA TAGATATGAT GCCTTAAGAG CTTGCATTGG AGACACTTTG TGTCAGAAAC 
     2401 TGCAAAATTT AAACATCTTC TTAGTAGGGT GTGGAGCCAT AGGCTGTGAA ATGTTGAAAA 
     2461 ATTTTGCTTT ACTTGGTGTT GGCACAAGCA AAGAGAAAGG AATGATTACA GTTACAGATC 
     2521 CTGACTTGAT AGAGAAATCC AACTTAAATA GACAGTTCCT ATTTCGTCCT CATCACATAC 
     2581 AGAAACCTAA AAGCTACACT GCTGCTGATG CTACTCTGAA AATAAATTCT CAAATAAAGA 
     2641 TAGATGCACA CCTGAACAAA GTATGTCCAA CCACTGAGAC CATTTACAAT GATGAGTTCT 
     2701 ATACTAAACA AGATGTAATT ATTACAGCAT TAGATAATGT GGAAGCCAGG AGATACGTAG 
     2761 ACAGTCGTTG CTTAGCAAAT CTAAGGCCTC TTTTAGATTC TGGAACAATG GGCACTAAGG 
     2821 GACACACTGA AGTTATTGTA CCGCATTTGA CTGAGTCTTA CAATAGTCAT CGGGATCCCC 
     2881 CAGAAGAGGA AATACCATTT TGTACTCTAA AATCCTTTCC AGCTGCTATT GAACATACCA 
     2941 TACAGTGGGC AAGAGATAAG TTTGAAAGTT CCTTTTCCCA CAAACCTTCA TTGTTTAACA 
     3001 AATTTTGGCA AACCTATTCA TCTGCAGAAG AAGTCTTACA GAAGATACAG AGTGGACACA 
     3061 GTTTAGAAGG CTGTTTTCAA GTTATAAAGT TACTTAGCAG AAGACCTAGA AATTGGTCCC 
     3121 AGTGTGTAGA ATTAGCAAGA TTAAAGTTTG AAAAATATTT TAACCATAAG GCTCTTCAGC 
     3181 TTCTTCACTG TTTCCCTCTG GACATACGAT TAAAAGATGG CAGTTTATTT TGGCAGTCAC 
     3241 CAAAGAGGCC ACCCTCTCCA ATAAAATTTG ATTTAAATGA GCCTTTGCAC CTCAGTTTCC 
     3301 TTCAGAATGC TGCAAAACTA TATGCTACAG TATATTGTAT TCCATTTGCA GAAGAGGACT 
     3361 TATCAGCAGA TGCCCTCTTG AATATTCTTT CAGAAGTAAA GATTCAGGAA TTCAAGCCTT 
     3421 CCAATAAGGT TGTTCAAACA GATGAAACTG CAAGGAAACC AGACCATGTT CCTATTAGCA 
     3481 GTGAAGATGA GAGGAATGCA ATTTTCCAAC TAGAAAAGGC TATTTTATCT AATGAAGCCA 
     3541 CCAAAAGTGA CCTTCAGATG GCAGTGCTTT CATTTGAAAA AGATGATGAT CATAATGGAC 
     3601 ACATAGATTT CATCACAGCT GCATCAAATC TTCGTGCCAA AATGTACAGC ATTGAACCAG 
     3661 CTGACCGTTT CAAAACAAAG CGCATAGCTG GTAAAATTAT ACCTGCTATA GCAACAACCA 
     3721 CTGCTACAGT TTCTGGCTTG GTTGCCTTGG AGATGATCAA AGTAACTGGT GGCTATCCAT 
     3781 TTGAAGCTTA CAAAAATTGT TTTCTTAACT TAGCCATTCC AATTGTAGTA TTTACAGAGA 
     3841 CAACTGAAGT AAGGAAAACT AAAATCAGAA ATGGAATATC ATTTACAATT TGGGATCGAT 
     3901 GGACCGTACA TGGAAAAGAA GATTTCACCC TCTTGGATTT CATAAATGCA GTCAAAGAGA 
     3961 AGTATGGAAT TGAGCCAACA ATGGTGGTAC AGGGAGTCAA AATGCTTTAT GTTCCTGTAA 
     4021 TGCCTGGTCA TGCAAAAAGA TTGAAGTTAA CAATGCATAA ACTTGTAAAA CCTACTACTG 
     4081 AAAAGAAATA TGTGGATCTT ACTGTGTCAT TTGCTCCAGA CATTGATGGA GATGAAGATT 
     4141 TGCCGGGACC TCCAGTAAGA TACTACTTCA GTCATGACAC TGATACGCGT ACGCGGCCGC 
     4201 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4261 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4321 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4381 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4441 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4501 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4561 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4621 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4681 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4741 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4801 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4861 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4921 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4981 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5521 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5761 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5821 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5881 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5941 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6001 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6061 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6121 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6181 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6241 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6301 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6361 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6421 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6481 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6541 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6601 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6661 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6721 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6781 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6841 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6901 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6961 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7021 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7081 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7141 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7201 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7261 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7441 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7621 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7681 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7741 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7801 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7861 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7921 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7981 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//