LOCUS RC214418 9866 bp DNA circular SYN DEFINITION SLC5A3 (Myc-DDK-tagged)-Human solute carrier family 5 (sodium/myo-inositol cotransporter), member 3 (SLC5A3) ACCESSION NM_006933 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 8157..8337 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 9065..9711 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 7032..7052 /label="LR50" misc_feature 5495..6223 /label="mGFP" misc_feature 6230..6295 /label="P2A" misc_feature 8385..9004 /label="pBR322 Ori" misc_feature 6296..6895 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 5474..5480 /label="Restriction Enzyme" misc_feature 3320..5474 /label="SLC5A3" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGAGCTGTA CTGGACACAG CAGACATTGC 3361 CATAGTGGCC CTGTATTTTA TCCTGGTCAT GTGCATTGGT TTTTTTGCCA TGTGGAAATC 3421 TAATAGAAGC ACCGTGAGTG GATACTTCCT GGCGGGGCGC TCTATGACCT GGGTAGCAAT 3481 TGGTGCCTCT CTGTTTGTGA GCAATATTGG GAGTGAGCAC TTCATTGGGC TGGCAGGATC 3541 TGGAGCTGCA AGTGGATTTG CAGTGGGCGC ATGGGAATTC AATGCCTTAC TGCTTTTACA 3601 ACTTCTGGGA TGGGTTTTCA TCCCAATTTA CATCCGGTCA GGGGTATATA CCATGCCTGA 3661 ATACTTGTCC AAGCGATTTG GTGGCCATAG GATTCAGGTC TATTTTGCAG CCTTGTCTCT 3721 GATTCTCTAT ATTTTCACCA AGCTCTCGGT GGATCTGTAT TCGGGTGCCC TTTTTATCCA 3781 GGAGTCTTTG GGTTGGAATC TTTATGTGTC TGTCATCCTG CTCATTGGCA TGACTGCTTT 3841 GCTGACTGTC ACCGGAGGCC TTGTTGCAGT GATCTACACA GACACTCTGC AGGCTCTGCT 3901 CATGATCATT GGGGCACTTA CACTTATGAT TATTAGCATA ATGGAGATTG GCGGGTTTGA 3961 GGAAGTTAAG AGAAGGTACA TGTTGGCCTC ACCCGATGTC ACTTCCATCT TATTGACATA 4021 CAACCTTTCC AACACAAATT CTTGTAATGT CTCCCCTAAG AAAGAAGCCC TGAAAATGCT 4081 GCGGAATCCA ACAGATGAAG ATGTTCCTTG GCCTGGATTC ATTCTTGGGC AGACCCCAGC 4141 TTCAGTATGG TACTGGTGTG CTGACCAAGT CATCGTGCAG AGGGTCCTTG CAGCCAAAAA 4201 CATTGCTCAT GCCAAAGGCT CTACTCTTAT GGCTGGCTTC TTAAAGCTCC TGCCAATGTT 4261 TATCATAGTT GTCCCAGGAA TGATTTCCAG GATACTGTTT ACTGATGATA TAGCTTGCAT 4321 CAACCCAGAG CACTGCATGC TGGTGTGTGG AAGCAGAGCT GGTTGCTCCA ATATTGCTTA 4381 CCCACGCCTG GTGATGAAGC TGGTTCCTGT GGGCCTTCGG GGTTTAATGA TGGCAGTGAT 4441 GATTGCAGCT CTGATGAGTG ACTTAGACTC TATCTTTAAC AGTGCCAGTA CCATATTCAC 4501 CCTCGATGTG TACAAACTTA TCCGCAAGAG CGCAAGCTCC CGGGAGTTAA TGATTGTGGG 4561 GAGGATATTT GTGGCATTTA TGGTGGTGAT CAGCATAGCA TGGGTGCCAA TCATCGTGGA 4621 GATGCAAGGA GGCCAGATGT ACCTTTACAT TCAGGAGGTA GCAGATTACC TGACACCCCC 4681 AGTGGCAGCC TTGTTCCTGC TGGCAATTTT CTGGAAGCGC TGCAATGAAC AAGGGGCTTT 4741 CTATGGTGGA ATGGCTGGCT TTGTTCTTGG AGCAGTCCGT TTGATACTGG CCTTTGCCTA 4801 CCGTGCCCCA GAATGTGACC AACCTGATAA TAGGCCGGGC TTCATCAAAG ACATCCATTA 4861 TATGTATGTG GCCACAGGAT TGTTTTGGGT CACGGGACTC ATTACTGTAA TTGTGAGCCT 4921 TCTCACACCA CCTCCCACAA AGGAACAGAT TCGAACCACC ACCTTTTGGT CTAAGAAGAA 4981 CCTGGTGGTG AAGGAGAACT GCTCCCCAAA AGAGGAACCA TACAAAATGC AAGAAAAGAG 5041 CATTCTGAGA TGCAGTGAGA ATAATGAGAC CATCAACCAC ATCATTCCCA ACGGGAAATC 5101 TGAAGACAGC ATTAAGGGCC TTCAGCCTGA AGATGTTAAT CTGTTGGTAA CCTGCAGAGA 5161 GGAGGGCAAC CCAGTGGCAT CCTTAGGTCA TTCAGAGGCA GAAACACCAG TTGACGCTTA 5221 CTCCAATGGG CAAGCAGCTC TCATGGGTGA GAAAGAGAGA AAGAAAGAAA CGGATGATGG 5281 AGGTCGGTAC TGGAAGTTCA TAGACTGGTT TTGTGGCTTT AAAAGTAAGA GCCTCAGCAA 5341 GAGGAGTCTC AGAGACCTGA TGGAAGAGGA GGCTGTTTGT TTACAGATGC TAGAAGAGAC 5401 TCGGCAAGTT AAAGTAATAC TAAATATTGG ACTTTTTGCT GTGTGTTCAC TTGGAATTTT 5461 CATGTTTGTT TATTTCTCCT TAACGCGTAC GCGGCCGCTC GAGATGAGCG GGGGCGAGGA 5521 GCTGTTCGCC GGCATCGTGC CCGTGCTGAT CGAGCTGGAC GGCGACGTGC ACGGCCACAA 5581 GTTCAGCGTG CGCGGCGAGG GCGAGGGCGA CGCCGACTAC GGCAAGCTGG AGATCAAGTT 5641 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTGGTGACCA CCCTCTGCTA 5701 CGGCATCCAG TGCTTCGCCC GCTACCCCGA GCACATGAAG ATGAACGACT TCTTCAAGAG 5761 CGCCATGCCC GAGGGCTACA TCCAGGAGCG CACCATCCAG TTCCAGGACG ACGGCAAGTA 5821 CAAGACCCGC GGCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 5881 GGGCAAGGAC TTCAAGGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAGCTTCAA 5941 CAGCCACAAC GTGTACATCC GCCCCGACAA GGCCAACAAC GGCCTGGAGG CTAACTTCAA 6001 GACCCGCCAC AACATCGAGG GCGGCGGCGT GCAGCTGGCC GACCACTACC AGACCAACGT 6061 GCCCCTGGGC GACGGCCCCG TGCTGATCCC CATCAACCAC TACCTGAGCA CTCAGACCAA 6121 GATCAGCAAG GACCGCAACG AGGCCCGCGA CCACATGGTG CTCCTGGAGT CCTTCAGCGC 6181 CTGCTGCCAC ACCCACGGCA TGGACGAGCT GTACAGGTCC GGACTCAGAG TTTGGGTAGG 6241 AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA GACGTGGAGG AGAACCCTGG 6301 ACCTATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC 6361 CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC 6421 GGATCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT 6481 CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC 6541 GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG 6601 CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA 6661 GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT 6721 GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT 6781 CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC 6841 CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC 6901 CTGATGTACA CCCAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA 6961 ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT 7021 AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA 7081 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 7141 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 7201 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 7261 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 7321 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 7381 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 7441 GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 7501 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 7561 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC 7621 TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA 7681 GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA 7741 GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC 7801 AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT 7861 CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC 7921 CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG 7981 TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC 8041 ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA 8101 GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC 8161 TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG 8221 AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT 8281 CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC 8341 TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 8401 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 8461 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 8521 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 8581 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 8641 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 8701 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 8761 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 8821 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG 8881 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 8941 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 9001 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 9061 ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA 9121 GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA 9181 TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT 9241 TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA 9301 CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG 9361 CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA 9421 GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC 9481 ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC 9541 GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA 9601 AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA 9661 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA 9721 TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 9781 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 9841 CATTTCCCCG AAAAGTGCCA CCTGAC //